Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0535 and Psest_0536 are separated by 55 nucleotides Psest_0536 and Psest_0537 are separated by 14 nucleotides
Psest_0535: Psest_0535 - glutamate-1-semialdehyde-2,1-aminomutase, at 587,985 to 589,268
_0535
Psest_0536: Psest_0536 - thiamine-phosphate pyrophosphorylase, at 589,324 to 589,965
_0536
Psest_0537: Psest_0537 - Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase, at 589,980 to 590,774
_0537
Position (kb) 589
590 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 589.332 kb on + strand at 589.333 kb on - strand at 589.333 kb on - strand at 589.584 kb on - strand, within Psest_0536 at 589.584 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.935 kb on - strand at 590.014 kb on + strand at 590.014 kb on + strand at 590.014 kb on + strand at 590.014 kb on + strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.062 kb on + strand, within Psest_0537 at 590.063 kb on - strand, within Psest_0537 at 590.210 kb on - strand, within Psest_0537 at 590.396 kb on - strand, within Psest_0537 at 590.788 kb on - strand at 590.788 kb on - strand at 590.910 kb on + strand at 590.911 kb on - strand at 590.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 589,332 + -0.2 589,333 - +1.8 589,333 - +0.7 589,584 - Psest_0536 0.40 -0.6 589,584 - Psest_0536 0.40 -1.3 589,887 - Psest_0536 0.88 -0.7 589,887 - Psest_0536 0.88 -1.2 589,887 - Psest_0536 0.88 +0.4 589,887 - Psest_0536 0.88 -0.4 589,887 - Psest_0536 0.88 -3.2 589,887 - Psest_0536 0.88 -0.5 589,887 - Psest_0536 0.88 -2.5 589,887 - Psest_0536 0.88 -0.5 589,887 - Psest_0536 0.88 -1.2 589,887 - Psest_0536 0.88 -1.0 589,935 - -0.5 590,014 + -1.1 590,014 + -1.4 590,014 + -0.2 590,014 + -0.5 590,015 - -1.0 590,015 - -1.4 590,015 - -0.5 590,015 - -0.9 590,015 - -1.3 590,015 - -0.8 590,015 - -0.5 590,015 - +1.5 590,062 + Psest_0537 0.10 -2.4 590,063 - Psest_0537 0.10 +0.2 590,210 - Psest_0537 0.29 -0.5 590,396 - Psest_0537 0.52 -1.0 590,788 - +0.3 590,788 - -0.4 590,910 + -0.5 590,911 - +2.2 590,911 - +2.2
Or see this region's nucleotide sequence