Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0422 and Psest_0423 overlap by 8 nucleotides Psest_0423 and Psest_0424 overlap by 8 nucleotides
Psest_0422: Psest_0422 - Site-specific recombinase XerD, at 457,937 to 459,058
_0422
Psest_0423: Psest_0423 - hypothetical protein, at 459,051 to 461,111
_0423
Psest_0424: Psest_0424 - Phage integrase family., at 461,104 to 462,975
_0424
Position (kb) 459
460
461
462 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 458.116 kb on + strand, within Psest_0422 at 458.116 kb on + strand, within Psest_0422 at 458.164 kb on - strand, within Psest_0422 at 458.192 kb on + strand, within Psest_0422 at 458.196 kb on + strand, within Psest_0422 at 458.253 kb on + strand, within Psest_0422 at 458.254 kb on - strand, within Psest_0422 at 458.310 kb on + strand, within Psest_0422 at 458.490 kb on + strand, within Psest_0422 at 458.503 kb on + strand, within Psest_0422 at 458.798 kb on - strand, within Psest_0422 at 458.798 kb on - strand, within Psest_0422 at 458.872 kb on + strand, within Psest_0422 at 458.872 kb on + strand, within Psest_0422 at 458.876 kb on + strand, within Psest_0422 at 458.891 kb on + strand, within Psest_0422 at 459.005 kb on + strand at 459.006 kb on - strand at 459.007 kb on + strand at 459.007 kb on + strand at 459.008 kb on - strand at 459.157 kb on - strand at 459.232 kb on - strand at 459.232 kb on - strand at 459.245 kb on + strand at 459.462 kb on + strand, within Psest_0423 at 459.630 kb on - strand, within Psest_0423 at 459.643 kb on + strand, within Psest_0423 at 459.644 kb on - strand, within Psest_0423 at 459.716 kb on + strand, within Psest_0423 at 459.749 kb on + strand, within Psest_0423 at 459.749 kb on + strand, within Psest_0423 at 459.750 kb on - strand, within Psest_0423 at 459.897 kb on + strand, within Psest_0423 at 459.918 kb on - strand, within Psest_0423 at 459.979 kb on + strand, within Psest_0423 at 459.979 kb on + strand, within Psest_0423 at 459.980 kb on - strand, within Psest_0423 at 460.093 kb on + strand, within Psest_0423 at 460.093 kb on + strand, within Psest_0423 at 460.094 kb on - strand, within Psest_0423 at 460.128 kb on + strand, within Psest_0423 at 460.131 kb on + strand, within Psest_0423 at 460.132 kb on - strand, within Psest_0423 at 460.149 kb on - strand, within Psest_0423 at 460.189 kb on + strand, within Psest_0423 at 460.320 kb on - strand, within Psest_0423 at 460.349 kb on + strand, within Psest_0423 at 460.419 kb on - strand, within Psest_0423 at 460.539 kb on + strand, within Psest_0423 at 460.540 kb on - strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.563 kb on + strand, within Psest_0423 at 460.563 kb on + strand, within Psest_0423 at 460.564 kb on - strand, within Psest_0423 at 460.740 kb on - strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.832 kb on + strand, within Psest_0423 at 460.871 kb on - strand, within Psest_0423 at 460.941 kb on - strand at 460.998 kb on + strand at 461.448 kb on + strand, within Psest_0424 at 461.598 kb on + strand, within Psest_0424 at 461.613 kb on + strand, within Psest_0424 at 461.664 kb on + strand, within Psest_0424 at 461.679 kb on + strand, within Psest_0424 at 461.744 kb on + strand, within Psest_0424 at 461.823 kb on - strand, within Psest_0424 at 461.869 kb on + strand, within Psest_0424 at 461.870 kb on - strand, within Psest_0424 at 461.919 kb on + strand, within Psest_0424 at 461.963 kb on - strand, within Psest_0424 at 461.987 kb on + strand, within Psest_0424 at 462.075 kb on + strand, within Psest_0424 at 462.075 kb on + strand, within Psest_0424
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 458,116 + Psest_0422 0.16 -1.4 458,116 + Psest_0422 0.16 -2.1 458,164 - Psest_0422 0.20 -1.1 458,192 + Psest_0422 0.23 +0.5 458,196 + Psest_0422 0.23 -0.3 458,253 + Psest_0422 0.28 -1.1 458,254 - Psest_0422 0.28 +0.1 458,310 + Psest_0422 0.33 -0.2 458,490 + Psest_0422 0.49 -0.2 458,503 + Psest_0422 0.50 +0.6 458,798 - Psest_0422 0.77 -1.1 458,798 - Psest_0422 0.77 +0.5 458,872 + Psest_0422 0.83 +0.6 458,872 + Psest_0422 0.83 -0.6 458,876 + Psest_0422 0.84 -1.9 458,891 + Psest_0422 0.85 +0.2 459,005 + -0.2 459,006 - -0.4 459,007 + -0.1 459,007 + -2.0 459,008 - -1.6 459,157 - -0.9 459,232 - -0.4 459,232 - -1.1 459,245 + -0.7 459,462 + Psest_0423 0.20 -1.0 459,630 - Psest_0423 0.28 -1.3 459,643 + Psest_0423 0.29 -0.9 459,644 - Psest_0423 0.29 -1.5 459,716 + Psest_0423 0.32 -1.1 459,749 + Psest_0423 0.34 -1.7 459,749 + Psest_0423 0.34 -0.5 459,750 - Psest_0423 0.34 -3.9 459,897 + Psest_0423 0.41 -0.1 459,918 - Psest_0423 0.42 -0.4 459,979 + Psest_0423 0.45 -1.1 459,979 + Psest_0423 0.45 -1.0 459,980 - Psest_0423 0.45 +0.2 460,093 + Psest_0423 0.51 -1.0 460,093 + Psest_0423 0.51 -1.5 460,094 - Psest_0423 0.51 -2.0 460,128 + Psest_0423 0.52 +2.1 460,131 + Psest_0423 0.52 +0.2 460,132 - Psest_0423 0.52 -0.7 460,149 - Psest_0423 0.53 -1.5 460,189 + Psest_0423 0.55 +1.1 460,320 - Psest_0423 0.62 -0.2 460,349 + Psest_0423 0.63 -0.7 460,419 - Psest_0423 0.66 -1.8 460,539 + Psest_0423 0.72 -0.6 460,540 - Psest_0423 0.72 -0.7 460,543 + Psest_0423 0.72 -1.2 460,543 + Psest_0423 0.72 -0.4 460,543 + Psest_0423 0.72 -0.2 460,563 + Psest_0423 0.73 +0.3 460,563 + Psest_0423 0.73 +0.7 460,564 - Psest_0423 0.73 +0.1 460,740 - Psest_0423 0.82 -1.6 460,822 + Psest_0423 0.86 +0.3 460,822 + Psest_0423 0.86 -0.6 460,822 + Psest_0423 0.86 -0.0 460,822 + Psest_0423 0.86 -0.5 460,823 - Psest_0423 0.86 -0.4 460,823 - Psest_0423 0.86 -0.2 460,823 - Psest_0423 0.86 +0.9 460,823 - Psest_0423 0.86 -0.3 460,832 + Psest_0423 0.86 -0.2 460,871 - Psest_0423 0.88 -1.4 460,941 - -0.4 460,998 + -0.4 461,448 + Psest_0424 0.18 +0.2 461,598 + Psest_0424 0.26 -2.1 461,613 + Psest_0424 0.27 -0.1 461,664 + Psest_0424 0.30 -0.1 461,679 + Psest_0424 0.31 -0.7 461,744 + Psest_0424 0.34 -0.4 461,823 - Psest_0424 0.38 -1.2 461,869 + Psest_0424 0.41 -0.6 461,870 - Psest_0424 0.41 -0.6 461,919 + Psest_0424 0.44 +0.1 461,963 - Psest_0424 0.46 -1.5 461,987 + Psest_0424 0.47 +0.4 462,075 + Psest_0424 0.52 +0.2 462,075 + Psest_0424 0.52 +0.8
Or see this region's nucleotide sequence