Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3573 and Psest_3574 are separated by 10 nucleotides Psest_3574 and Psest_3575 are separated by 164 nucleotides Psest_3575 and Psest_3576 are separated by 131 nucleotides
Psest_3573: Psest_3573 - dTDP-4-dehydrorhamnose reductase, at 3,747,142 to 3,748,026
_3573
Psest_3574: Psest_3574 - single stranded DNA-binding protein (ssb), at 3,748,037 to 3,748,531
_3574
Psest_3575: Psest_3575 - Arabinose efflux permease, at 3,748,696 to 3,750,063
_3575
Psest_3576: Psest_3576 - excinuclease ABC, A subunit, at 3,750,195 to 3,753,038
_3576
Position (kb)
3748
3749
3750
3751 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3747.840 kb on - strand, within Psest_3573 at 3747.840 kb on - strand, within Psest_3573 at 3747.843 kb on - strand, within Psest_3573 at 3747.843 kb on - strand, within Psest_3573 at 3747.843 kb on - strand, within Psest_3573 at 3747.846 kb on - strand, within Psest_3573 at 3747.846 kb on - strand, within Psest_3573 at 3747.846 kb on - strand, within Psest_3573 at 3748.039 kb on - strand at 3748.039 kb on - strand at 3748.039 kb on - strand at 3748.039 kb on - strand at 3748.039 kb on - strand at 3748.039 kb on - strand at 3748.560 kb on - strand at 3748.616 kb on - strand at 3748.616 kb on - strand at 3748.755 kb on - strand at 3748.869 kb on + strand, within Psest_3575 at 3748.877 kb on + strand, within Psest_3575 at 3748.877 kb on + strand, within Psest_3575 at 3748.877 kb on + strand, within Psest_3575 at 3748.877 kb on + strand, within Psest_3575 at 3748.877 kb on + strand, within Psest_3575 at 3748.877 kb on + strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3748.878 kb on - strand, within Psest_3575 at 3749.189 kb on - strand, within Psest_3575 at 3749.250 kb on + strand, within Psest_3575 at 3749.250 kb on + strand, within Psest_3575 at 3749.250 kb on + strand, within Psest_3575 at 3749.250 kb on + strand, within Psest_3575 at 3749.251 kb on - strand, within Psest_3575 at 3749.886 kb on - strand, within Psest_3575 at 3749.886 kb on - strand, within Psest_3575 at 3749.943 kb on - strand at 3750.051 kb on - strand at 3750.051 kb on - strand at 3750.079 kb on + strand at 3750.080 kb on - strand at 3750.080 kb on - strand at 3750.098 kb on + strand at 3750.160 kb on + strand at 3750.160 kb on + strand at 3750.160 kb on + strand at 3750.161 kb on - strand at 3750.161 kb on - strand at 3750.161 kb on - strand at 3750.161 kb on - strand at 3750.161 kb on - strand at 3750.372 kb on + strand at 3750.507 kb on + strand, within Psest_3576 at 3750.507 kb on + strand, within Psest_3576 at 3750.507 kb on + strand, within Psest_3576 at 3750.507 kb on + strand, within Psest_3576 at 3750.507 kb on + strand, within Psest_3576 at 3750.507 kb on + strand, within Psest_3576 at 3750.507 kb on + strand, within Psest_3576 at 3750.508 kb on - strand, within Psest_3576 at 3750.508 kb on - strand, within Psest_3576 at 3750.508 kb on - strand, within Psest_3576 at 3750.528 kb on + strand, within Psest_3576 at 3750.528 kb on + strand, within Psest_3576 at 3750.529 kb on - strand, within Psest_3576 at 3750.529 kb on - strand, within Psest_3576 at 3750.529 kb on - strand, within Psest_3576 at 3750.529 kb on - strand, within Psest_3576 at 3750.745 kb on - strand, within Psest_3576 at 3750.745 kb on - strand, within Psest_3576 at 3750.760 kb on + strand, within Psest_3576 at 3750.760 kb on + strand, within Psest_3576 at 3750.760 kb on + strand, within Psest_3576 at 3750.761 kb on - strand, within Psest_3576 at 3750.802 kb on + strand, within Psest_3576 at 3750.803 kb on - strand, within Psest_3576 at 3750.803 kb on - strand, within Psest_3576
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 3,747,840 - Psest_3573 0.79 +0.2 3,747,840 - Psest_3573 0.79 -2.0 3,747,843 - Psest_3573 0.79 +0.2 3,747,843 - Psest_3573 0.79 -0.7 3,747,843 - Psest_3573 0.79 -2.2 3,747,846 - Psest_3573 0.80 -0.8 3,747,846 - Psest_3573 0.80 -2.8 3,747,846 - Psest_3573 0.80 -1.1 3,748,039 - -1.3 3,748,039 - -0.2 3,748,039 - +0.2 3,748,039 - +0.7 3,748,039 - -0.6 3,748,039 - +0.8 3,748,560 - -0.2 3,748,616 - -0.5 3,748,616 - -0.1 3,748,755 - -0.7 3,748,869 + Psest_3575 0.13 -0.5 3,748,877 + Psest_3575 0.13 -1.6 3,748,877 + Psest_3575 0.13 -1.1 3,748,877 + Psest_3575 0.13 -2.2 3,748,877 + Psest_3575 0.13 -0.9 3,748,877 + Psest_3575 0.13 -0.9 3,748,877 + Psest_3575 0.13 -1.9 3,748,878 - Psest_3575 0.13 -0.5 3,748,878 - Psest_3575 0.13 +0.8 3,748,878 - Psest_3575 0.13 +0.1 3,748,878 - Psest_3575 0.13 -0.2 3,748,878 - Psest_3575 0.13 -0.1 3,748,878 - Psest_3575 0.13 -0.1 3,748,878 - Psest_3575 0.13 -0.6 3,748,878 - Psest_3575 0.13 -0.2 3,748,878 - Psest_3575 0.13 +0.2 3,749,189 - Psest_3575 0.36 -2.0 3,749,250 + Psest_3575 0.40 -0.5 3,749,250 + Psest_3575 0.40 -0.8 3,749,250 + Psest_3575 0.40 -0.8 3,749,250 + Psest_3575 0.40 +1.1 3,749,251 - Psest_3575 0.41 -1.2 3,749,886 - Psest_3575 0.87 -2.7 3,749,886 - Psest_3575 0.87 +1.4 3,749,943 - -0.6 3,750,051 - +0.7 3,750,051 - +0.2 3,750,079 + -1.0 3,750,080 - -0.9 3,750,080 - -0.6 3,750,098 + -0.9 3,750,160 + +0.6 3,750,160 + +0.3 3,750,160 + -0.3 3,750,161 - -0.5 3,750,161 - -0.9 3,750,161 - -1.5 3,750,161 - -0.9 3,750,161 - -0.4 3,750,372 + +0.7 3,750,507 + Psest_3576 0.11 -0.5 3,750,507 + Psest_3576 0.11 -1.6 3,750,507 + Psest_3576 0.11 -1.0 3,750,507 + Psest_3576 0.11 -1.6 3,750,507 + Psest_3576 0.11 -0.6 3,750,507 + Psest_3576 0.11 +0.5 3,750,507 + Psest_3576 0.11 -1.3 3,750,508 - Psest_3576 0.11 +0.1 3,750,508 - Psest_3576 0.11 +0.5 3,750,508 - Psest_3576 0.11 +0.2 3,750,528 + Psest_3576 0.12 +1.5 3,750,528 + Psest_3576 0.12 -1.4 3,750,529 - Psest_3576 0.12 +0.1 3,750,529 - Psest_3576 0.12 -0.3 3,750,529 - Psest_3576 0.12 +0.6 3,750,529 - Psest_3576 0.12 +1.4 3,750,745 - Psest_3576 0.19 -0.3 3,750,745 - Psest_3576 0.19 +0.2 3,750,760 + Psest_3576 0.20 +0.1 3,750,760 + Psest_3576 0.20 -0.8 3,750,760 + Psest_3576 0.20 -0.0 3,750,761 - Psest_3576 0.20 -0.2 3,750,802 + Psest_3576 0.21 -0.5 3,750,803 - Psest_3576 0.21 +0.5 3,750,803 - Psest_3576 0.21 +0.2
Or see this region's nucleotide sequence