Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3246 and Psest_3247 are separated by 58 nucleotides Psest_3247 and Psest_3248 are separated by 14 nucleotides Psest_3248 and Psest_3249 are separated by 115 nucleotides Psest_3249 and Psest_3250 are separated by 58 nucleotides Psest_3250 and Psest_3251 are separated by 9 nucleotides
Psest_3246: Psest_3246 - Protein of unknown function (DUF2914)., at 3,416,081 to 3,417,103
_3246
Psest_3247: Psest_3247 - Uncharacterized membrane protein, at 3,417,162 to 3,417,680
_3247
Psest_3248: Psest_3248 - Glucose/sorbosone dehydrogenases, at 3,417,695 to 3,419,026
_3248
Psest_3249: Psest_3249 - SmpA / OmlA family., at 3,419,142 to 3,419,483
_3249
Psest_3250: Psest_3250 - Rho termination factor, N-terminal domain., at 3,419,542 to 3,419,940
_3250
Psest_3251: Psest_3251 - hypothetical protein, at 3,419,950 to 3,420,081
_3251
Position (kb)
3417
3418
3419
3420 Strain fitness (log2 ratio)
-2
-1
0
1 at 3416.867 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.909 kb on + strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3416.910 kb on - strand, within Psest_3246 at 3417.132 kb on + strand at 3417.133 kb on - strand at 3417.458 kb on + strand, within Psest_3247 at 3417.458 kb on + strand, within Psest_3247 at 3417.572 kb on - strand, within Psest_3247 at 3417.679 kb on - strand at 3417.739 kb on - strand at 3417.739 kb on - strand at 3417.739 kb on - strand at 3417.804 kb on - strand at 3417.922 kb on - strand, within Psest_3248 at 3418.205 kb on + strand, within Psest_3248 at 3418.205 kb on + strand, within Psest_3248 at 3418.205 kb on + strand, within Psest_3248 at 3418.206 kb on - strand, within Psest_3248 at 3418.206 kb on - strand, within Psest_3248 at 3418.209 kb on + strand, within Psest_3248 at 3418.293 kb on + strand, within Psest_3248 at 3418.294 kb on - strand, within Psest_3248 at 3418.294 kb on - strand, within Psest_3248 at 3418.361 kb on + strand, within Psest_3248 at 3418.361 kb on + strand, within Psest_3248 at 3418.362 kb on - strand, within Psest_3248 at 3418.362 kb on - strand, within Psest_3248 at 3418.362 kb on - strand, within Psest_3248 at 3418.580 kb on + strand, within Psest_3248 at 3418.580 kb on + strand, within Psest_3248 at 3418.580 kb on + strand, within Psest_3248 at 3418.580 kb on + strand, within Psest_3248 at 3418.581 kb on - strand, within Psest_3248 at 3418.581 kb on - strand, within Psest_3248 at 3418.581 kb on - strand, within Psest_3248 at 3418.610 kb on + strand, within Psest_3248 at 3418.622 kb on + strand, within Psest_3248 at 3418.629 kb on - strand, within Psest_3248 at 3418.629 kb on - strand, within Psest_3248 at 3418.629 kb on - strand, within Psest_3248 at 3418.629 kb on - strand, within Psest_3248 at 3418.629 kb on - strand, within Psest_3248 at 3418.629 kb on - strand, within Psest_3248 at 3418.704 kb on - strand, within Psest_3248 at 3418.745 kb on + strand, within Psest_3248 at 3418.745 kb on + strand, within Psest_3248 at 3418.745 kb on + strand, within Psest_3248 at 3418.745 kb on + strand, within Psest_3248 at 3418.745 kb on + strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.746 kb on - strand, within Psest_3248 at 3418.833 kb on - strand, within Psest_3248 at 3419.037 kb on + strand at 3419.038 kb on - strand at 3419.038 kb on - strand at 3419.108 kb on - strand at 3419.108 kb on - strand at 3419.108 kb on - strand at 3419.114 kb on + strand at 3419.114 kb on + strand at 3419.114 kb on + strand at 3419.115 kb on - strand at 3419.115 kb on - strand at 3419.115 kb on - strand at 3419.115 kb on - strand at 3419.115 kb on - strand at 3419.160 kb on + strand at 3419.160 kb on + strand at 3419.160 kb on + strand at 3419.161 kb on - strand at 3419.161 kb on - strand at 3419.161 kb on - strand at 3419.206 kb on + strand, within Psest_3249 at 3419.206 kb on + strand, within Psest_3249 at 3419.206 kb on + strand, within Psest_3249 at 3419.206 kb on + strand, within Psest_3249 at 3419.207 kb on - strand, within Psest_3249 at 3419.270 kb on + strand, within Psest_3249 at 3419.271 kb on - strand, within Psest_3249 at 3419.271 kb on - strand, within Psest_3249 at 3419.355 kb on + strand, within Psest_3249 at 3419.355 kb on + strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.356 kb on - strand, within Psest_3249 at 3419.389 kb on - strand, within Psest_3249 at 3419.389 kb on - strand, within Psest_3249 at 3419.391 kb on + strand, within Psest_3249 at 3419.391 kb on + strand, within Psest_3249 at 3419.391 kb on + strand, within Psest_3249 at 3419.391 kb on + strand, within Psest_3249 at 3419.391 kb on + strand, within Psest_3249 at 3419.391 kb on + strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.392 kb on - strand, within Psest_3249 at 3419.566 kb on + strand at 3419.566 kb on + strand at 3419.566 kb on + strand at 3419.567 kb on - strand at 3419.612 kb on - strand, within Psest_3250 at 3419.829 kb on + strand, within Psest_3250 at 3419.830 kb on - strand, within Psest_3250 at 3419.830 kb on - strand, within Psest_3250 at 3419.830 kb on - strand, within Psest_3250 at 3419.839 kb on - strand, within Psest_3250 at 3419.861 kb on + strand, within Psest_3250 at 3419.862 kb on - strand, within Psest_3250 at 3419.862 kb on - strand, within Psest_3250 at 3419.910 kb on + strand at 3419.911 kb on - strand at 3419.921 kb on + strand at 3419.922 kb on - strand at 3419.943 kb on + strand at 3419.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 3,416,867 + Psest_3246 0.77 -0.0 3,416,909 + Psest_3246 0.81 +0.2 3,416,909 + Psest_3246 0.81 +0.5 3,416,909 + Psest_3246 0.81 +0.3 3,416,909 + Psest_3246 0.81 -0.3 3,416,909 + Psest_3246 0.81 +0.2 3,416,909 + Psest_3246 0.81 +1.4 3,416,909 + Psest_3246 0.81 +0.6 3,416,909 + Psest_3246 0.81 -0.5 3,416,909 + Psest_3246 0.81 -0.0 3,416,910 - Psest_3246 0.81 +0.3 3,416,910 - Psest_3246 0.81 +0.3 3,416,910 - Psest_3246 0.81 -0.1 3,416,910 - Psest_3246 0.81 +1.1 3,416,910 - Psest_3246 0.81 +0.4 3,416,910 - Psest_3246 0.81 +0.2 3,416,910 - Psest_3246 0.81 +0.5 3,417,132 + -1.2 3,417,133 - -0.9 3,417,458 + Psest_3247 0.57 -0.1 3,417,458 + Psest_3247 0.57 +0.1 3,417,572 - Psest_3247 0.79 -0.5 3,417,679 - +0.1 3,417,739 - +0.1 3,417,739 - -0.9 3,417,739 - +0.5 3,417,804 - +0.3 3,417,922 - Psest_3248 0.17 -0.4 3,418,205 + Psest_3248 0.38 -0.6 3,418,205 + Psest_3248 0.38 -0.8 3,418,205 + Psest_3248 0.38 -0.3 3,418,206 - Psest_3248 0.38 -0.0 3,418,206 - Psest_3248 0.38 +1.6 3,418,209 + Psest_3248 0.39 -0.2 3,418,293 + Psest_3248 0.45 +1.4 3,418,294 - Psest_3248 0.45 +0.5 3,418,294 - Psest_3248 0.45 -0.0 3,418,361 + Psest_3248 0.50 +0.5 3,418,361 + Psest_3248 0.50 +0.7 3,418,362 - Psest_3248 0.50 +0.5 3,418,362 - Psest_3248 0.50 -0.3 3,418,362 - Psest_3248 0.50 +0.6 3,418,580 + Psest_3248 0.66 +0.7 3,418,580 + Psest_3248 0.66 +1.0 3,418,580 + Psest_3248 0.66 +0.6 3,418,580 + Psest_3248 0.66 +0.3 3,418,581 - Psest_3248 0.67 -0.4 3,418,581 - Psest_3248 0.67 +1.3 3,418,581 - Psest_3248 0.67 +0.8 3,418,610 + Psest_3248 0.69 +1.4 3,418,622 + Psest_3248 0.70 +0.5 3,418,629 - Psest_3248 0.70 +0.8 3,418,629 - Psest_3248 0.70 -0.6 3,418,629 - Psest_3248 0.70 +0.0 3,418,629 - Psest_3248 0.70 +0.5 3,418,629 - Psest_3248 0.70 +0.3 3,418,629 - Psest_3248 0.70 -1.4 3,418,704 - Psest_3248 0.76 +1.3 3,418,745 + Psest_3248 0.79 +0.1 3,418,745 + Psest_3248 0.79 +0.7 3,418,745 + Psest_3248 0.79 -0.1 3,418,745 + Psest_3248 0.79 +0.2 3,418,745 + Psest_3248 0.79 +0.1 3,418,746 - Psest_3248 0.79 +0.4 3,418,746 - Psest_3248 0.79 +0.3 3,418,746 - Psest_3248 0.79 +1.1 3,418,746 - Psest_3248 0.79 +1.0 3,418,746 - Psest_3248 0.79 +1.4 3,418,746 - Psest_3248 0.79 +0.4 3,418,746 - Psest_3248 0.79 +0.5 3,418,833 - Psest_3248 0.85 +0.8 3,419,037 + -0.2 3,419,038 - +0.6 3,419,038 - -0.1 3,419,108 - +0.6 3,419,108 - +0.4 3,419,108 - +0.3 3,419,114 + +0.1 3,419,114 + -1.4 3,419,114 + -1.0 3,419,115 - +1.5 3,419,115 - +0.4 3,419,115 - +1.1 3,419,115 - -1.3 3,419,115 - -0.2 3,419,160 + -1.2 3,419,160 + +0.2 3,419,160 + +0.5 3,419,161 - +0.5 3,419,161 - +0.6 3,419,161 - -0.6 3,419,206 + Psest_3249 0.19 -2.2 3,419,206 + Psest_3249 0.19 +1.6 3,419,206 + Psest_3249 0.19 +0.1 3,419,206 + Psest_3249 0.19 -0.7 3,419,207 - Psest_3249 0.19 +0.1 3,419,270 + Psest_3249 0.37 +0.5 3,419,271 - Psest_3249 0.38 -1.3 3,419,271 - Psest_3249 0.38 -0.0 3,419,355 + Psest_3249 0.62 -0.1 3,419,355 + Psest_3249 0.62 -0.5 3,419,356 - Psest_3249 0.63 +0.3 3,419,356 - Psest_3249 0.63 +0.2 3,419,356 - Psest_3249 0.63 -0.5 3,419,356 - Psest_3249 0.63 +0.9 3,419,356 - Psest_3249 0.63 +0.2 3,419,356 - Psest_3249 0.63 -1.1 3,419,356 - Psest_3249 0.63 -0.6 3,419,389 - Psest_3249 0.72 +0.1 3,419,389 - Psest_3249 0.72 -0.4 3,419,391 + Psest_3249 0.73 +0.5 3,419,391 + Psest_3249 0.73 +0.6 3,419,391 + Psest_3249 0.73 -0.4 3,419,391 + Psest_3249 0.73 +1.5 3,419,391 + Psest_3249 0.73 +0.2 3,419,391 + Psest_3249 0.73 -0.0 3,419,392 - Psest_3249 0.73 -0.4 3,419,392 - Psest_3249 0.73 -0.4 3,419,392 - Psest_3249 0.73 -0.6 3,419,392 - Psest_3249 0.73 +0.2 3,419,392 - Psest_3249 0.73 +0.2 3,419,392 - Psest_3249 0.73 -0.1 3,419,392 - Psest_3249 0.73 +0.3 3,419,392 - Psest_3249 0.73 +0.2 3,419,566 + -1.2 3,419,566 + -0.3 3,419,566 + -0.7 3,419,567 - +0.1 3,419,612 - Psest_3250 0.18 +0.7 3,419,829 + Psest_3250 0.72 -0.7 3,419,830 - Psest_3250 0.72 +0.7 3,419,830 - Psest_3250 0.72 -0.8 3,419,830 - Psest_3250 0.72 -0.0 3,419,839 - Psest_3250 0.74 -0.9 3,419,861 + Psest_3250 0.80 -1.3 3,419,862 - Psest_3250 0.80 -0.1 3,419,862 - Psest_3250 0.80 -0.4 3,419,910 + -0.4 3,419,911 - -0.6 3,419,921 + -0.1 3,419,922 - +0.1 3,419,943 + +0.0 3,419,944 - -2.6
Or see this region's nucleotide sequence