Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0232 and Psest_0233 are separated by 98 nucleotides Psest_0233 and Psest_0234 are separated by 67 nucleotides
Psest_0232: Psest_0232 - Predicted dehydrogenase, at 240,529 to 241,647
_0232
Psest_0233: Psest_0233 - hypothetical protein, at 241,746 to 243,479
_0233
Psest_0234: Psest_0234 - dihydroxy-acid dehydratase, at 243,547 to 245,385
_0234
Position (kb) 241
242
243
244 Strain fitness (log2 ratio) -1
0
1
2 at 240.923 kb on + strand, within Psest_0232 at 240.923 kb on + strand, within Psest_0232 at 240.923 kb on + strand, within Psest_0232 at 240.923 kb on + strand, within Psest_0232 at 240.923 kb on + strand, within Psest_0232 at 240.923 kb on + strand, within Psest_0232 at 240.923 kb on + strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 240.924 kb on - strand, within Psest_0232 at 241.155 kb on - strand, within Psest_0232 at 241.222 kb on + strand, within Psest_0232 at 241.222 kb on + strand, within Psest_0232 at 241.222 kb on + strand, within Psest_0232 at 241.223 kb on - strand, within Psest_0232 at 241.400 kb on - strand, within Psest_0232 at 241.783 kb on - strand at 241.783 kb on - strand at 241.783 kb on - strand at 241.783 kb on - strand at 241.783 kb on - strand at 241.783 kb on - strand at 241.801 kb on + strand at 241.802 kb on - strand at 242.028 kb on + strand, within Psest_0233 at 242.029 kb on - strand, within Psest_0233 at 242.031 kb on + strand, within Psest_0233 at 242.031 kb on + strand, within Psest_0233 at 242.031 kb on + strand, within Psest_0233 at 242.031 kb on + strand, within Psest_0233 at 242.032 kb on - strand, within Psest_0233 at 242.032 kb on - strand, within Psest_0233 at 242.032 kb on - strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.085 kb on + strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.086 kb on - strand, within Psest_0233 at 242.143 kb on - strand, within Psest_0233 at 242.179 kb on + strand, within Psest_0233 at 242.179 kb on + strand, within Psest_0233 at 242.300 kb on + strand, within Psest_0233 at 242.653 kb on - strand, within Psest_0233 at 242.951 kb on - strand, within Psest_0233 at 243.084 kb on + strand, within Psest_0233 at 243.084 kb on + strand, within Psest_0233 at 243.119 kb on + strand, within Psest_0233 at 243.119 kb on + strand, within Psest_0233 at 243.232 kb on + strand, within Psest_0233 at 243.232 kb on + strand, within Psest_0233 at 243.232 kb on + strand, within Psest_0233 at 243.232 kb on + strand, within Psest_0233 at 243.232 kb on + strand, within Psest_0233 at 243.233 kb on - strand, within Psest_0233 at 243.233 kb on - strand, within Psest_0233 at 243.233 kb on - strand, within Psest_0233 at 243.233 kb on - strand, within Psest_0233 at 243.233 kb on - strand, within Psest_0233 at 243.233 kb on - strand, within Psest_0233 at 243.406 kb on + strand at 243.407 kb on - strand at 243.526 kb on + strand at 243.526 kb on + strand at 243.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 240,923 + Psest_0232 0.35 +1.4 240,923 + Psest_0232 0.35 +0.6 240,923 + Psest_0232 0.35 -0.2 240,923 + Psest_0232 0.35 +1.2 240,923 + Psest_0232 0.35 +1.5 240,923 + Psest_0232 0.35 +2.5 240,923 + Psest_0232 0.35 +1.3 240,924 - Psest_0232 0.35 +1.1 240,924 - Psest_0232 0.35 -0.5 240,924 - Psest_0232 0.35 +0.2 240,924 - Psest_0232 0.35 +1.0 240,924 - Psest_0232 0.35 +1.8 240,924 - Psest_0232 0.35 +0.7 240,924 - Psest_0232 0.35 +0.2 241,155 - Psest_0232 0.56 -0.4 241,222 + Psest_0232 0.62 +1.0 241,222 + Psest_0232 0.62 -1.0 241,222 + Psest_0232 0.62 +1.8 241,223 - Psest_0232 0.62 -0.2 241,400 - Psest_0232 0.78 +1.7 241,783 - +0.4 241,783 - -0.2 241,783 - +0.9 241,783 - +0.0 241,783 - +0.4 241,783 - +0.4 241,801 + +0.9 241,802 - -0.2 242,028 + Psest_0233 0.16 +0.7 242,029 - Psest_0233 0.16 +0.7 242,031 + Psest_0233 0.16 +0.5 242,031 + Psest_0233 0.16 +0.8 242,031 + Psest_0233 0.16 +0.4 242,031 + Psest_0233 0.16 +0.8 242,032 - Psest_0233 0.16 -0.2 242,032 - Psest_0233 0.16 -0.2 242,032 - Psest_0233 0.16 -0.9 242,085 + Psest_0233 0.20 +1.3 242,085 + Psest_0233 0.20 +0.7 242,085 + Psest_0233 0.20 -0.5 242,085 + Psest_0233 0.20 +1.2 242,085 + Psest_0233 0.20 -0.0 242,085 + Psest_0233 0.20 +0.1 242,085 + Psest_0233 0.20 +0.7 242,085 + Psest_0233 0.20 +1.2 242,085 + Psest_0233 0.20 +0.7 242,085 + Psest_0233 0.20 +0.9 242,085 + Psest_0233 0.20 +1.3 242,086 - Psest_0233 0.20 -0.2 242,086 - Psest_0233 0.20 -0.8 242,086 - Psest_0233 0.20 +0.6 242,086 - Psest_0233 0.20 -1.5 242,086 - Psest_0233 0.20 -1.5 242,086 - Psest_0233 0.20 +0.4 242,086 - Psest_0233 0.20 -1.1 242,086 - Psest_0233 0.20 -0.2 242,086 - Psest_0233 0.20 +0.4 242,143 - Psest_0233 0.23 +0.4 242,179 + Psest_0233 0.25 +0.2 242,179 + Psest_0233 0.25 +1.0 242,300 + Psest_0233 0.32 +0.2 242,653 - Psest_0233 0.52 -0.2 242,951 - Psest_0233 0.69 +0.2 243,084 + Psest_0233 0.77 -0.2 243,084 + Psest_0233 0.77 +1.0 243,119 + Psest_0233 0.79 +1.0 243,119 + Psest_0233 0.79 +0.4 243,232 + Psest_0233 0.86 +1.2 243,232 + Psest_0233 0.86 -0.7 243,232 + Psest_0233 0.86 +0.6 243,232 + Psest_0233 0.86 +1.2 243,232 + Psest_0233 0.86 +0.5 243,233 - Psest_0233 0.86 +1.8 243,233 - Psest_0233 0.86 -0.1 243,233 - Psest_0233 0.86 +0.9 243,233 - Psest_0233 0.86 +0.2 243,233 - Psest_0233 0.86 +0.7 243,233 - Psest_0233 0.86 +1.2 243,406 + -0.2 243,407 - +0.7 243,526 + -0.3 243,526 + +0.4 243,527 - +0.7
Or see this region's nucleotide sequence