Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2348 and Psest_2349 are separated by 11 nucleotides Psest_2349 and Psest_2351 are separated by 158 nucleotides Psest_2351 and Psest_2352 are separated by 98 nucleotides
Psest_2348: Psest_2348 - urea carboxylase-associated protein 2, at 2,501,847 to 2,502,575
_2348
Psest_2349: Psest_2349 - urea carboxylase-associated protein 1, at 2,502,587 to 2,503,222
_2349
Psest_2351: Psest_2351 - urea carboxylase, at 2,503,381 to 2,506,980
_2351
Psest_2352: Psest_2352 - allophanate hydrolase, at 2,507,079 to 2,508,881
_2352
Position (kb)
2503
2504
2505
2506
2507 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2502.384 kb on + strand, within Psest_2348 at 2502.450 kb on + strand, within Psest_2348 at 2502.568 kb on - strand at 2502.568 kb on - strand at 2502.568 kb on - strand at 2502.803 kb on + strand, within Psest_2349 at 2502.803 kb on + strand, within Psest_2349 at 2502.828 kb on - strand, within Psest_2349 at 2502.948 kb on - strand, within Psest_2349 at 2502.948 kb on - strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.106 kb on + strand, within Psest_2349 at 2503.107 kb on - strand, within Psest_2349 at 2503.164 kb on + strand at 2503.165 kb on - strand at 2503.340 kb on - strand at 2503.558 kb on + strand at 2503.981 kb on + strand, within Psest_2351 at 2503.981 kb on + strand, within Psest_2351 at 2503.981 kb on + strand, within Psest_2351 at 2503.981 kb on + strand, within Psest_2351 at 2503.982 kb on - strand, within Psest_2351 at 2503.982 kb on - strand, within Psest_2351 at 2504.010 kb on - strand, within Psest_2351 at 2504.010 kb on - strand, within Psest_2351 at 2504.010 kb on - strand, within Psest_2351 at 2504.015 kb on + strand, within Psest_2351 at 2504.015 kb on + strand, within Psest_2351 at 2504.015 kb on + strand, within Psest_2351 at 2504.015 kb on + strand, within Psest_2351 at 2504.015 kb on + strand, within Psest_2351 at 2504.016 kb on - strand, within Psest_2351 at 2504.016 kb on - strand, within Psest_2351 at 2504.016 kb on - strand, within Psest_2351 at 2504.210 kb on - strand, within Psest_2351 at 2504.233 kb on + strand, within Psest_2351 at 2504.233 kb on + strand, within Psest_2351 at 2504.233 kb on + strand, within Psest_2351 at 2504.404 kb on + strand, within Psest_2351 at 2504.404 kb on + strand, within Psest_2351 at 2504.405 kb on - strand, within Psest_2351 at 2504.494 kb on - strand, within Psest_2351 at 2504.630 kb on - strand, within Psest_2351 at 2504.653 kb on + strand, within Psest_2351 at 2504.728 kb on + strand, within Psest_2351 at 2504.729 kb on - strand, within Psest_2351 at 2504.729 kb on - strand, within Psest_2351 at 2504.729 kb on - strand, within Psest_2351 at 2504.729 kb on - strand, within Psest_2351 at 2505.079 kb on + strand, within Psest_2351 at 2505.079 kb on + strand, within Psest_2351 at 2505.080 kb on - strand, within Psest_2351 at 2505.080 kb on - strand, within Psest_2351 at 2505.080 kb on - strand, within Psest_2351 at 2505.115 kb on + strand, within Psest_2351 at 2505.115 kb on + strand, within Psest_2351 at 2505.116 kb on - strand, within Psest_2351 at 2505.289 kb on + strand, within Psest_2351 at 2505.313 kb on + strand, within Psest_2351 at 2505.314 kb on - strand, within Psest_2351 at 2505.328 kb on + strand, within Psest_2351 at 2505.328 kb on + strand, within Psest_2351 at 2505.328 kb on + strand, within Psest_2351 at 2505.329 kb on - strand, within Psest_2351 at 2505.331 kb on - strand, within Psest_2351 at 2505.475 kb on + strand, within Psest_2351 at 2505.729 kb on - strand, within Psest_2351 at 2505.909 kb on + strand, within Psest_2351 at 2505.909 kb on + strand, within Psest_2351 at 2505.910 kb on - strand, within Psest_2351 at 2505.910 kb on - strand, within Psest_2351 at 2505.941 kb on - strand, within Psest_2351 at 2506.049 kb on + strand, within Psest_2351 at 2506.050 kb on - strand, within Psest_2351 at 2506.050 kb on - strand, within Psest_2351 at 2506.096 kb on + strand, within Psest_2351 at 2506.096 kb on + strand, within Psest_2351 at 2506.096 kb on + strand, within Psest_2351 at 2506.096 kb on + strand, within Psest_2351 at 2506.096 kb on + strand, within Psest_2351 at 2506.096 kb on + strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.097 kb on - strand, within Psest_2351 at 2506.189 kb on + strand, within Psest_2351 at 2506.214 kb on - strand, within Psest_2351 at 2506.214 kb on - strand, within Psest_2351 at 2506.214 kb on - strand, within Psest_2351 at 2506.214 kb on - strand, within Psest_2351 at 2506.214 kb on - strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.238 kb on + strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.239 kb on - strand, within Psest_2351 at 2506.240 kb on + strand, within Psest_2351 at 2506.241 kb on - strand, within Psest_2351 at 2506.300 kb on + strand, within Psest_2351 at 2506.300 kb on + strand, within Psest_2351 at 2506.301 kb on - strand, within Psest_2351 at 2506.301 kb on - strand, within Psest_2351 at 2506.301 kb on - strand, within Psest_2351 at 2506.301 kb on - strand, within Psest_2351 at 2506.301 kb on - strand, within Psest_2351 at 2506.301 kb on - strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.354 kb on + strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.355 kb on - strand, within Psest_2351 at 2506.367 kb on - strand, within Psest_2351 at 2506.495 kb on + strand, within Psest_2351 at 2507.065 kb on - strand at 2507.168 kb on - strand at 2507.168 kb on - strand at 2507.318 kb on + strand, within Psest_2352 at 2507.367 kb on + strand, within Psest_2352 at 2507.367 kb on + strand, within Psest_2352 at 2507.368 kb on - strand, within Psest_2352 at 2507.369 kb on + strand, within Psest_2352 at 2507.370 kb on - strand, within Psest_2352 at 2507.370 kb on - strand, within Psest_2352 at 2507.370 kb on - strand, within Psest_2352 at 2507.787 kb on + strand, within Psest_2352 at 2507.788 kb on - strand, within Psest_2352 at 2507.788 kb on - strand, within Psest_2352
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 2,502,384 + Psest_2348 0.74 -1.0 2,502,450 + Psest_2348 0.83 -0.5 2,502,568 - +0.1 2,502,568 - +0.6 2,502,568 - -0.1 2,502,803 + Psest_2349 0.34 -0.5 2,502,803 + Psest_2349 0.34 -1.9 2,502,828 - Psest_2349 0.38 +0.5 2,502,948 - Psest_2349 0.57 -0.2 2,502,948 - Psest_2349 0.57 +0.2 2,503,106 + Psest_2349 0.82 +0.6 2,503,106 + Psest_2349 0.82 +0.4 2,503,106 + Psest_2349 0.82 +0.5 2,503,106 + Psest_2349 0.82 +0.6 2,503,106 + Psest_2349 0.82 +1.3 2,503,106 + Psest_2349 0.82 -0.1 2,503,106 + Psest_2349 0.82 +0.5 2,503,107 - Psest_2349 0.82 -0.4 2,503,164 + -0.4 2,503,165 - +0.5 2,503,340 - +0.1 2,503,558 + +0.5 2,503,981 + Psest_2351 0.17 -1.4 2,503,981 + Psest_2351 0.17 +0.8 2,503,981 + Psest_2351 0.17 +1.0 2,503,981 + Psest_2351 0.17 +0.2 2,503,982 - Psest_2351 0.17 -0.5 2,503,982 - Psest_2351 0.17 -0.5 2,504,010 - Psest_2351 0.17 +0.4 2,504,010 - Psest_2351 0.17 +0.3 2,504,010 - Psest_2351 0.17 -0.5 2,504,015 + Psest_2351 0.18 +0.7 2,504,015 + Psest_2351 0.18 -0.2 2,504,015 + Psest_2351 0.18 +0.7 2,504,015 + Psest_2351 0.18 -0.9 2,504,015 + Psest_2351 0.18 +0.3 2,504,016 - Psest_2351 0.18 +0.3 2,504,016 - Psest_2351 0.18 -0.3 2,504,016 - Psest_2351 0.18 -0.3 2,504,210 - Psest_2351 0.23 +0.0 2,504,233 + Psest_2351 0.24 -0.0 2,504,233 + Psest_2351 0.24 +1.1 2,504,233 + Psest_2351 0.24 +0.3 2,504,404 + Psest_2351 0.28 +1.6 2,504,404 + Psest_2351 0.28 +0.4 2,504,405 - Psest_2351 0.28 -0.0 2,504,494 - Psest_2351 0.31 +0.1 2,504,630 - Psest_2351 0.35 +0.7 2,504,653 + Psest_2351 0.35 -0.5 2,504,728 + Psest_2351 0.37 -0.0 2,504,729 - Psest_2351 0.37 -0.3 2,504,729 - Psest_2351 0.37 +0.2 2,504,729 - Psest_2351 0.37 +1.3 2,504,729 - Psest_2351 0.37 +1.0 2,505,079 + Psest_2351 0.47 +0.7 2,505,079 + Psest_2351 0.47 -0.0 2,505,080 - Psest_2351 0.47 +1.0 2,505,080 - Psest_2351 0.47 -1.2 2,505,080 - Psest_2351 0.47 +0.5 2,505,115 + Psest_2351 0.48 +1.3 2,505,115 + Psest_2351 0.48 +1.3 2,505,116 - Psest_2351 0.48 -0.2 2,505,289 + Psest_2351 0.53 +0.6 2,505,313 + Psest_2351 0.54 +0.6 2,505,314 - Psest_2351 0.54 +1.0 2,505,328 + Psest_2351 0.54 -1.5 2,505,328 + Psest_2351 0.54 +0.1 2,505,328 + Psest_2351 0.54 -0.6 2,505,329 - Psest_2351 0.54 +0.7 2,505,331 - Psest_2351 0.54 +1.0 2,505,475 + Psest_2351 0.58 +0.8 2,505,729 - Psest_2351 0.65 -0.4 2,505,909 + Psest_2351 0.70 +0.0 2,505,909 + Psest_2351 0.70 +0.4 2,505,910 - Psest_2351 0.70 +0.1 2,505,910 - Psest_2351 0.70 +1.3 2,505,941 - Psest_2351 0.71 +0.9 2,506,049 + Psest_2351 0.74 +0.9 2,506,050 - Psest_2351 0.74 +1.1 2,506,050 - Psest_2351 0.74 +0.4 2,506,096 + Psest_2351 0.75 +0.9 2,506,096 + Psest_2351 0.75 -0.5 2,506,096 + Psest_2351 0.75 +0.1 2,506,096 + Psest_2351 0.75 +1.4 2,506,096 + Psest_2351 0.75 +1.1 2,506,096 + Psest_2351 0.75 +0.9 2,506,097 - Psest_2351 0.75 +1.0 2,506,097 - Psest_2351 0.75 +0.7 2,506,097 - Psest_2351 0.75 +1.5 2,506,097 - Psest_2351 0.75 +0.7 2,506,097 - Psest_2351 0.75 +1.5 2,506,097 - Psest_2351 0.75 +0.5 2,506,097 - Psest_2351 0.75 +0.7 2,506,097 - Psest_2351 0.75 -1.0 2,506,097 - Psest_2351 0.75 +0.3 2,506,097 - Psest_2351 0.75 +0.7 2,506,097 - Psest_2351 0.75 +1.3 2,506,097 - Psest_2351 0.75 +0.7 2,506,189 + Psest_2351 0.78 -0.9 2,506,214 - Psest_2351 0.79 +0.4 2,506,214 - Psest_2351 0.79 +1.9 2,506,214 - Psest_2351 0.79 +0.4 2,506,214 - Psest_2351 0.79 +1.3 2,506,214 - Psest_2351 0.79 -1.4 2,506,238 + Psest_2351 0.79 -0.1 2,506,238 + Psest_2351 0.79 +0.3 2,506,238 + Psest_2351 0.79 +0.5 2,506,238 + Psest_2351 0.79 -0.4 2,506,238 + Psest_2351 0.79 +0.2 2,506,238 + Psest_2351 0.79 +1.3 2,506,238 + +1.3 2,506,238 + Psest_2351 0.79 -0.2 2,506,238 + Psest_2351 0.79 +0.3 2,506,238 + Psest_2351 0.79 +0.2 2,506,238 + Psest_2351 0.79 +0.2 2,506,238 + Psest_2351 0.79 +0.7 2,506,238 + Psest_2351 0.79 +0.6 2,506,238 + Psest_2351 0.79 -0.1 2,506,238 + Psest_2351 0.79 -0.4 2,506,238 + Psest_2351 0.79 +0.6 2,506,238 + Psest_2351 0.79 -0.0 2,506,238 + Psest_2351 0.79 -0.0 2,506,238 + Psest_2351 0.79 -0.6 2,506,239 - Psest_2351 0.79 -0.2 2,506,239 - Psest_2351 0.79 +1.0 2,506,239 - Psest_2351 0.79 +0.9 2,506,239 - Psest_2351 0.79 +0.5 2,506,239 - Psest_2351 0.79 -0.9 2,506,239 - Psest_2351 0.79 +0.8 2,506,239 - Psest_2351 0.79 +1.0 2,506,239 - Psest_2351 0.79 +0.4 2,506,240 + Psest_2351 0.79 +1.3 2,506,241 - Psest_2351 0.79 -0.2 2,506,300 + Psest_2351 0.81 -1.5 2,506,300 + Psest_2351 0.81 +0.4 2,506,301 - Psest_2351 0.81 -0.1 2,506,301 - Psest_2351 0.81 -0.1 2,506,301 - Psest_2351 0.81 -0.6 2,506,301 - Psest_2351 0.81 +0.8 2,506,301 - Psest_2351 0.81 -2.0 2,506,301 - Psest_2351 0.81 +0.1 2,506,354 + Psest_2351 0.83 +1.7 2,506,354 + Psest_2351 0.83 -0.1 2,506,354 + Psest_2351 0.83 +1.7 2,506,354 + Psest_2351 0.83 -0.7 2,506,354 + Psest_2351 0.83 -0.7 2,506,354 + Psest_2351 0.83 -0.6 2,506,354 + Psest_2351 0.83 +1.4 2,506,355 - Psest_2351 0.83 +1.6 2,506,355 - Psest_2351 0.83 +1.0 2,506,355 - Psest_2351 0.83 -0.5 2,506,355 - Psest_2351 0.83 +1.3 2,506,355 - Psest_2351 0.83 -1.0 2,506,355 - Psest_2351 0.83 -1.5 2,506,355 - Psest_2351 0.83 +0.8 2,506,367 - Psest_2351 0.83 +1.0 2,506,495 + Psest_2351 0.86 +0.6 2,507,065 - +0.5 2,507,168 - -1.6 2,507,168 - +1.5 2,507,318 + Psest_2352 0.13 +0.5 2,507,367 + Psest_2352 0.16 +0.7 2,507,367 + Psest_2352 0.16 +1.1 2,507,368 - Psest_2352 0.16 +0.5 2,507,369 + Psest_2352 0.16 +1.1 2,507,370 - Psest_2352 0.16 +0.4 2,507,370 - Psest_2352 0.16 +1.4 2,507,370 - Psest_2352 0.16 +1.5 2,507,787 + Psest_2352 0.39 -0.0 2,507,788 - Psest_2352 0.39 +0.9 2,507,788 - Psest_2352 0.39 -0.4
Or see this region's nucleotide sequence