Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1449 and Psest_1450 are separated by 8 nucleotides Psest_1450 and Psest_1451 are separated by 157 nucleotides Psest_1451 and Psest_1452 are separated by 160 nucleotides
Psest_1449: Psest_1449 - Short-chain dehydrogenases of various substrate specificities, at 1,557,384 to 1,558,358
_1449
Psest_1450: Psest_1450 - Transcriptional regulator, at 1,558,367 to 1,559,293
_1450
Psest_1451: Psest_1451 - Uncharacterized ABC-type transport system, permease components, at 1,559,451 to 1,560,041
_1451
Psest_1452: Psest_1452 - 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase, at 1,560,202 to 1,562,511
_1452
Position (kb)
1558
1559
1560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1557.418 kb on + strand at 1557.745 kb on - strand, within Psest_1449 at 1557.774 kb on + strand, within Psest_1449 at 1557.775 kb on - strand, within Psest_1449 at 1557.932 kb on + strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.991 kb on - strand at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1558.082 kb on + strand, within Psest_1449 at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.401 kb on - strand at 1559.452 kb on + strand at 1559.474 kb on + strand at 1559.474 kb on + strand at 1559.474 kb on + strand at 1559.475 kb on - strand at 1559.475 kb on - strand at 1559.475 kb on - strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.477 kb on - strand at 1559.477 kb on - strand at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.744 kb on - strand, within Psest_1451 at 1559.744 kb on - strand, within Psest_1451 at 1560.105 kb on + strand at 1560.105 kb on + strand at 1560.106 kb on - strand at 1560.106 kb on - strand at 1560.124 kb on + strand at 1560.128 kb on + strand at 1560.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 1,557,418 + +0.6 1,557,745 - Psest_1449 0.37 -0.6 1,557,774 + Psest_1449 0.40 +0.4 1,557,775 - Psest_1449 0.40 -0.4 1,557,932 + Psest_1449 0.56 +0.1 1,557,933 - Psest_1449 0.56 +0.1 1,557,933 - Psest_1449 0.56 -1.4 1,557,933 - Psest_1449 0.56 -0.3 1,557,933 - Psest_1449 0.56 +0.8 1,557,990 + Psest_1449 0.62 -0.2 1,557,990 + Psest_1449 0.62 +0.6 1,557,990 + Psest_1449 0.62 +0.7 1,557,990 + Psest_1449 0.62 -0.1 1,557,990 + Psest_1449 0.62 -0.6 1,557,990 + Psest_1449 0.62 -0.3 1,557,990 + Psest_1449 0.62 -0.4 1,557,990 + Psest_1449 0.62 -0.4 1,557,990 + Psest_1449 0.62 +0.7 1,557,991 - +0.6 1,557,991 - Psest_1449 0.62 -0.9 1,557,991 - Psest_1449 0.62 -2.5 1,557,991 - Psest_1449 0.62 -0.7 1,557,991 - Psest_1449 0.62 -0.8 1,557,991 - Psest_1449 0.62 -0.4 1,557,991 - Psest_1449 0.62 -0.7 1,557,991 - Psest_1449 0.62 +0.8 1,557,991 - Psest_1449 0.62 +0.0 1,558,082 + Psest_1449 0.72 +0.3 1,559,302 - -1.3 1,559,302 - -3.2 1,559,302 - -0.7 1,559,302 - +0.6 1,559,302 - -2.7 1,559,302 - -4.2 1,559,302 - -1.3 1,559,401 - -1.2 1,559,452 + -1.0 1,559,474 + -0.5 1,559,474 + -0.4 1,559,474 + -1.3 1,559,475 - -0.4 1,559,475 - +0.3 1,559,475 - +0.5 1,559,476 + -1.8 1,559,476 + -1.1 1,559,476 + +0.2 1,559,476 + +0.2 1,559,476 + -0.1 1,559,476 + -0.8 1,559,476 + -0.3 1,559,476 + -0.2 1,559,476 + +1.1 1,559,477 - -0.3 1,559,477 - -0.0 1,559,743 + Psest_1451 0.49 +0.8 1,559,743 + Psest_1451 0.49 -0.4 1,559,743 + Psest_1451 0.49 +0.1 1,559,743 + Psest_1451 0.49 +0.1 1,559,744 - Psest_1451 0.50 -2.3 1,559,744 - Psest_1451 0.50 -1.6 1,560,105 + -2.2 1,560,105 + -2.1 1,560,106 - -2.1 1,560,106 - -1.6 1,560,124 + -1.8 1,560,128 + -2.2 1,560,129 - -0.9
Or see this region's nucleotide sequence