Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0919 and Psest_0920 are separated by 137 nucleotides Psest_0920 and Psest_0921 are separated by 76 nucleotides
Psest_0919: Psest_0919 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 999,671 to 1,000,627
_0919
Psest_0920: Psest_0920 - Transcriptional regulators, at 1,000,765 to 1,001,250
_0920
Psest_0921: Psest_0921 - AraC-type DNA-binding domain-containing proteins, at 1,001,327 to 1,002,154
_0921
Position (kb)
1000
1001
1002 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1000.123 kb on + strand, within Psest_0919 at 1000.124 kb on - strand, within Psest_0919 at 1000.124 kb on - strand, within Psest_0919 at 1000.260 kb on + strand, within Psest_0919 at 1000.570 kb on + strand at 1000.571 kb on - strand at 1000.607 kb on + strand at 1000.608 kb on - strand at 1000.608 kb on - strand at 1000.608 kb on - strand at 1000.643 kb on + strand at 1000.644 kb on - strand at 1000.644 kb on - strand at 1000.647 kb on + strand at 1000.648 kb on - strand at 1000.648 kb on - strand at 1000.648 kb on - strand at 1000.648 kb on - strand at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.997 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.060 kb on - strand, within Psest_0920 at 1001.060 kb on - strand, within Psest_0920 at 1001.083 kb on + strand, within Psest_0920 at 1001.084 kb on - strand, within Psest_0920 at 1001.084 kb on - strand, within Psest_0920 at 1001.204 kb on - strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.287 kb on - strand at 1001.287 kb on - strand at 1001.289 kb on + strand at 1001.290 kb on - strand at 1001.290 kb on - strand at 1001.290 kb on - strand at 1001.313 kb on + strand at 1001.313 kb on + strand at 1001.313 kb on + strand at 1001.314 kb on - strand at 1001.314 kb on - strand at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.499 kb on + strand, within Psest_0921 at 1001.500 kb on - strand, within Psest_0921 at 1001.500 kb on - strand, within Psest_0921 at 1001.971 kb on + strand, within Psest_0921 at 1001.971 kb on + strand, within Psest_0921 at 1001.972 kb on - strand, within Psest_0921 at 1001.989 kb on - strand, within Psest_0921 at 1002.099 kb on + strand at 1002.099 kb on + strand at 1002.099 kb on + strand at 1002.100 kb on - strand at 1002.102 kb on + strand at 1002.102 kb on + strand at 1002.102 kb on + strand at 1002.102 kb on + strand at 1002.102 kb on + strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.103 kb on - strand at 1002.106 kb on + strand at 1002.106 kb on + strand at 1002.186 kb on + strand at 1002.186 kb on + strand at 1002.187 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 1,000,123 + Psest_0919 0.47 -0.2 1,000,124 - Psest_0919 0.47 +1.3 1,000,124 - Psest_0919 0.47 +0.3 1,000,260 + Psest_0919 0.62 +0.3 1,000,570 + +1.5 1,000,571 - +0.1 1,000,607 + +0.5 1,000,608 - -0.5 1,000,608 - -1.2 1,000,608 - +0.1 1,000,643 + -1.2 1,000,644 - -3.9 1,000,644 - -3.2 1,000,647 + -0.1 1,000,648 - -1.6 1,000,648 - -3.1 1,000,648 - -3.4 1,000,648 - -5.0 1,000,967 + Psest_0920 0.42 -0.8 1,000,967 + Psest_0920 0.42 -0.2 1,000,967 + Psest_0920 0.42 -0.0 1,000,967 + Psest_0920 0.42 +0.5 1,000,967 + Psest_0920 0.42 +0.4 1,000,967 + Psest_0920 0.42 +0.1 1,000,967 + Psest_0920 0.42 -1.8 1,000,967 + Psest_0920 0.42 -0.7 1,000,968 - Psest_0920 0.42 +0.0 1,000,968 - Psest_0920 0.42 -0.5 1,000,968 - Psest_0920 0.42 +0.0 1,000,968 - Psest_0920 0.42 -0.7 1,000,968 - Psest_0920 0.42 +0.3 1,000,968 - Psest_0920 0.42 -0.3 1,000,968 - Psest_0920 0.42 +0.8 1,000,997 + Psest_0920 0.48 -0.2 1,001,059 + Psest_0920 0.60 -0.7 1,001,059 + Psest_0920 0.60 +0.7 1,001,059 + Psest_0920 0.60 -0.8 1,001,059 + Psest_0920 0.60 -0.2 1,001,059 + Psest_0920 0.60 +0.5 1,001,060 - Psest_0920 0.61 +0.2 1,001,060 - Psest_0920 0.61 -0.6 1,001,083 + Psest_0920 0.65 -0.7 1,001,084 - Psest_0920 0.66 -1.2 1,001,084 - Psest_0920 0.66 +0.4 1,001,204 - -0.4 1,001,286 + -0.8 1,001,286 + -0.8 1,001,286 + +1.4 1,001,286 + -0.3 1,001,286 + -0.8 1,001,286 + -0.0 1,001,287 - -0.1 1,001,287 - +0.7 1,001,289 + -0.6 1,001,290 - -0.1 1,001,290 - -0.5 1,001,290 - +0.6 1,001,313 + -0.2 1,001,313 + -0.6 1,001,313 + -0.6 1,001,314 - +0.6 1,001,314 - -0.0 1,001,495 + Psest_0921 0.20 -1.4 1,001,495 + Psest_0921 0.20 +0.3 1,001,495 + Psest_0921 0.20 -0.0 1,001,495 + Psest_0921 0.20 -0.1 1,001,495 + Psest_0921 0.20 -0.5 1,001,496 - Psest_0921 0.20 +0.6 1,001,496 - Psest_0921 0.20 -0.5 1,001,496 - Psest_0921 0.20 +0.4 1,001,496 - Psest_0921 0.20 +0.2 1,001,496 - Psest_0921 0.20 +0.1 1,001,496 - Psest_0921 0.20 +0.5 1,001,496 - Psest_0921 0.20 -0.3 1,001,499 + Psest_0921 0.21 +0.5 1,001,500 - Psest_0921 0.21 -0.6 1,001,500 - Psest_0921 0.21 -1.1 1,001,971 + Psest_0921 0.78 -1.7 1,001,971 + Psest_0921 0.78 -0.9 1,001,972 - Psest_0921 0.78 +0.0 1,001,989 - Psest_0921 0.80 -0.0 1,002,099 + +0.2 1,002,099 + -0.2 1,002,099 + -0.6 1,002,100 - -1.0 1,002,102 + +0.6 1,002,102 + -0.1 1,002,102 + -0.2 1,002,102 + +0.2 1,002,102 + +0.1 1,002,103 - -0.0 1,002,103 - -1.6 1,002,103 - +0.1 1,002,103 - +0.3 1,002,103 - +0.2 1,002,103 - +1.2 1,002,103 - -0.6 1,002,103 - -0.3 1,002,103 - -0.4 1,002,106 + +0.5 1,002,106 + +0.8 1,002,186 + +0.0 1,002,186 + +1.2 1,002,187 - -1.5
Or see this region's nucleotide sequence