Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3837 and Psest_3838 are separated by 20 nucleotides Psest_3838 and Psest_3839 are separated by 73 nucleotides Psest_3839 and Psest_3840 are separated by 6 nucleotides
Psest_3837: Psest_3837 - malate synthase G, at 4,007,300 to 4,009,480
_3837
Psest_3838: Psest_3838 - Uncharacterized protein, possibly involved in utilization of glycolate and propanediol, at 4,009,501 to 4,009,905
_3838
Psest_3839: Psest_3839 - D-lactate/glycolate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6; EC 1.1.99.14) (from data) , at 4,009,979 to 4,011,196
_3839
Psest_3840: Psest_3840 - D-lactate/glycolate dehydrogenase, FAD binding subunit GlcE (EC 1.1.99.6; EC 1.1.99.14) (from data) , at 4,011,203 to 4,012,264
_3840
Position (kb)
4009
4010
4011
4012 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4009.065 kb on + strand, within Psest_3837 at 4009.065 kb on + strand, within Psest_3837 at 4009.105 kb on - strand, within Psest_3837 at 4009.739 kb on + strand, within Psest_3838 at 4009.740 kb on - strand, within Psest_3838 at 4009.831 kb on - strand, within Psest_3838 at 4009.925 kb on + strand at 4009.926 kb on - strand at 4009.926 kb on - strand at 4009.926 kb on - strand at 4009.926 kb on - strand at 4010.157 kb on + strand, within Psest_3839 at 4010.158 kb on - strand, within Psest_3839 at 4010.556 kb on + strand, within Psest_3839 at 4010.557 kb on - strand, within Psest_3839 at 4010.926 kb on - strand, within Psest_3839 at 4011.039 kb on + strand, within Psest_3839 at 4011.040 kb on - strand, within Psest_3839 at 4011.040 kb on - strand, within Psest_3839 at 4011.084 kb on + strand at 4011.196 kb on + strand at 4011.196 kb on + strand at 4011.196 kb on + strand at 4011.196 kb on + strand at 4011.197 kb on - strand at 4011.197 kb on - strand at 4011.197 kb on - strand at 4011.197 kb on - strand at 4011.197 kb on - strand at 4011.197 kb on - strand at 4011.197 kb on - strand at 4011.216 kb on + strand at 4011.216 kb on + strand at 4011.216 kb on + strand at 4011.216 kb on + strand at 4011.216 kb on + strand at 4011.217 kb on - strand at 4011.217 kb on - strand at 4011.217 kb on - strand at 4011.217 kb on - strand at 4011.217 kb on - strand at 4011.217 kb on - strand at 4011.722 kb on + strand, within Psest_3840 at 4011.723 kb on - strand, within Psest_3840 at 4012.095 kb on + strand, within Psest_3840 at 4012.095 kb on + strand, within Psest_3840 at 4012.096 kb on - strand, within Psest_3840 at 4012.096 kb on - strand, within Psest_3840 at 4012.096 kb on - strand, within Psest_3840 at 4012.133 kb on + strand, within Psest_3840 at 4012.133 kb on + strand, within Psest_3840 at 4012.134 kb on - strand, within Psest_3840
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 4,009,065 + Psest_3837 0.81 +0.7 4,009,065 + Psest_3837 0.81 -0.8 4,009,105 - Psest_3837 0.83 +0.4 4,009,739 + Psest_3838 0.59 -0.8 4,009,740 - Psest_3838 0.59 +0.5 4,009,831 - Psest_3838 0.81 +1.5 4,009,925 + -2.2 4,009,926 - +2.8 4,009,926 - +2.0 4,009,926 - +1.3 4,009,926 - +1.3 4,010,157 + Psest_3839 0.15 +1.5 4,010,158 - Psest_3839 0.15 +1.0 4,010,556 + Psest_3839 0.47 +0.4 4,010,557 - Psest_3839 0.47 +0.8 4,010,926 - Psest_3839 0.78 +0.3 4,011,039 + Psest_3839 0.87 -0.4 4,011,040 - Psest_3839 0.87 +1.3 4,011,040 - Psest_3839 0.87 -0.4 4,011,084 + +1.3 4,011,196 + +0.0 4,011,196 + -0.2 4,011,196 + -0.7 4,011,196 + -1.5 4,011,197 - -0.6 4,011,197 - +2.8 4,011,197 - +0.8 4,011,197 - +0.4 4,011,197 - +1.6 4,011,197 - +1.8 4,011,197 - +0.2 4,011,216 + +0.3 4,011,216 + +0.5 4,011,216 + -0.2 4,011,216 + +0.8 4,011,216 + -1.1 4,011,217 - +1.7 4,011,217 - +0.5 4,011,217 - +1.1 4,011,217 - +1.3 4,011,217 - +1.5 4,011,217 - +2.6 4,011,722 + Psest_3840 0.49 -0.1 4,011,723 - Psest_3840 0.49 +1.6 4,012,095 + Psest_3840 0.84 -0.2 4,012,095 + Psest_3840 0.84 +1.4 4,012,096 - Psest_3840 0.84 +0.8 4,012,096 - Psest_3840 0.84 +0.5 4,012,096 - Psest_3840 0.84 +0.7 4,012,133 + Psest_3840 0.88 -0.6 4,012,133 + Psest_3840 0.88 +0.6 4,012,134 - Psest_3840 0.88 +0.9
Or see this region's nucleotide sequence