Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3224 and Psest_3226 are separated by 261 nucleotides Psest_3226 and Psest_3227 are separated by 85 nucleotides Psest_3227 and Psest_3228 are separated by 22 nucleotides
Psest_3224: Psest_3224 - 16S RNA G1207 methylase RsmC, at 3,396,898 to 3,397,896
_3224
Psest_3226: Psest_3226 - Predicted membrane protein, at 3,398,158 to 3,398,745
_3226
Psest_3227: Psest_3227 - Predicted ester cyclase, at 3,398,831 to 3,399,274
_3227
Psest_3228: Psest_3228 - Zn-dependent protease with chaperone function, at 3,399,297 to 3,400,115
_3228
Position (kb)
3398
3399 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3397.193 kb on - strand, within Psest_3224 at 3397.407 kb on - strand, within Psest_3224 at 3397.445 kb on + strand, within Psest_3224 at 3397.445 kb on + strand, within Psest_3224 at 3397.445 kb on + strand, within Psest_3224 at 3397.466 kb on + strand, within Psest_3224 at 3397.466 kb on + strand, within Psest_3224 at 3397.467 kb on - strand, within Psest_3224 at 3397.467 kb on - strand, within Psest_3224 at 3397.467 kb on - strand, within Psest_3224 at 3397.717 kb on + strand, within Psest_3224 at 3397.717 kb on + strand, within Psest_3224 at 3397.778 kb on + strand, within Psest_3224 at 3397.778 kb on + strand, within Psest_3224 at 3397.779 kb on - strand, within Psest_3224 at 3397.779 kb on - strand, within Psest_3224 at 3397.779 kb on - strand, within Psest_3224 at 3397.877 kb on - strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.004 kb on + strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.005 kb on - strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.087 kb on + strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.088 kb on - strand at 3398.144 kb on + strand at 3398.183 kb on + strand at 3398.183 kb on + strand at 3398.183 kb on + strand at 3398.184 kb on - strand at 3398.278 kb on - strand, within Psest_3226 at 3398.781 kb on - strand at 3398.869 kb on + strand at 3398.927 kb on + strand, within Psest_3227 at 3398.927 kb on + strand, within Psest_3227 at 3398.927 kb on + strand, within Psest_3227 at 3398.927 kb on + strand, within Psest_3227 at 3398.928 kb on - strand, within Psest_3227 at 3398.928 kb on - strand, within Psest_3227 at 3398.928 kb on - strand, within Psest_3227 at 3398.928 kb on - strand, within Psest_3227 at 3398.928 kb on - strand, within Psest_3227 at 3398.928 kb on - strand, within Psest_3227 at 3399.005 kb on - strand, within Psest_3227 at 3399.067 kb on + strand, within Psest_3227 at 3399.068 kb on - strand, within Psest_3227 at 3399.068 kb on - strand, within Psest_3227 at 3399.527 kb on + strand, within Psest_3228 at 3399.548 kb on - strand, within Psest_3228 at 3399.631 kb on + strand, within Psest_3228 at 3399.631 kb on + strand, within Psest_3228 at 3399.631 kb on + strand, within Psest_3228 at 3399.632 kb on - strand, within Psest_3228 at 3399.632 kb on - strand, within Psest_3228 at 3399.632 kb on - strand, within Psest_3228 at 3399.745 kb on + strand, within Psest_3228 at 3399.745 kb on + strand, within Psest_3228 at 3399.745 kb on + strand, within Psest_3228
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 3,397,193 - Psest_3224 0.30 -0.3 3,397,407 - Psest_3224 0.51 -0.2 3,397,445 + Psest_3224 0.55 +0.3 3,397,445 + Psest_3224 0.55 -0.5 3,397,445 + Psest_3224 0.55 -1.0 3,397,466 + Psest_3224 0.57 -0.6 3,397,466 + Psest_3224 0.57 -1.1 3,397,467 - Psest_3224 0.57 +0.6 3,397,467 - Psest_3224 0.57 +0.3 3,397,467 - Psest_3224 0.57 -2.6 3,397,717 + Psest_3224 0.82 -0.1 3,397,717 + Psest_3224 0.82 -0.6 3,397,778 + Psest_3224 0.88 +0.8 3,397,778 + Psest_3224 0.88 +0.0 3,397,779 - Psest_3224 0.88 +0.6 3,397,779 - Psest_3224 0.88 -0.5 3,397,779 - Psest_3224 0.88 +0.3 3,397,877 - +0.1 3,398,004 + -0.1 3,398,004 + -0.9 3,398,004 + -0.8 3,398,004 + -0.5 3,398,004 + +0.5 3,398,004 + +0.7 3,398,004 + -0.4 3,398,004 + +0.1 3,398,004 + +1.0 3,398,004 + +1.9 3,398,005 - -3.9 3,398,005 - -4.2 3,398,005 - -2.4 3,398,005 - -4.6 3,398,005 - -3.9 3,398,005 - -3.5 3,398,005 - -3.9 3,398,005 - -4.3 3,398,005 - -4.0 3,398,005 - -3.1 3,398,087 + -0.9 3,398,087 + -1.2 3,398,087 + +0.1 3,398,087 + +0.8 3,398,087 + -0.2 3,398,087 + +0.6 3,398,087 + +0.0 3,398,087 + +0.8 3,398,088 - -4.5 3,398,088 - -3.0 3,398,088 - -0.3 3,398,088 - -2.6 3,398,088 - -4.0 3,398,088 - -1.2 3,398,088 - -1.1 3,398,088 - -3.8 3,398,088 - -2.5 3,398,088 - -3.6 3,398,088 - -3.7 3,398,144 + -0.7 3,398,183 + -4.4 3,398,183 + -3.0 3,398,183 + -5.0 3,398,184 - -2.9 3,398,278 - Psest_3226 0.20 -4.7 3,398,781 - -1.0 3,398,869 + -0.6 3,398,927 + Psest_3227 0.22 -0.6 3,398,927 + Psest_3227 0.22 -1.3 3,398,927 + Psest_3227 0.22 +0.1 3,398,927 + Psest_3227 0.22 -0.2 3,398,928 - Psest_3227 0.22 -1.4 3,398,928 - Psest_3227 0.22 -0.7 3,398,928 - Psest_3227 0.22 -0.1 3,398,928 - Psest_3227 0.22 -0.1 3,398,928 - Psest_3227 0.22 +0.0 3,398,928 - Psest_3227 0.22 -0.9 3,399,005 - Psest_3227 0.39 -0.7 3,399,067 + Psest_3227 0.53 -0.5 3,399,068 - Psest_3227 0.53 +1.1 3,399,068 - Psest_3227 0.53 -0.5 3,399,527 + Psest_3228 0.28 -0.9 3,399,548 - Psest_3228 0.31 -2.1 3,399,631 + Psest_3228 0.41 -1.7 3,399,631 + Psest_3228 0.41 -2.9 3,399,631 + Psest_3228 0.41 +0.4 3,399,632 - Psest_3228 0.41 -0.8 3,399,632 - Psest_3228 0.41 +0.0 3,399,632 - Psest_3228 0.41 +0.2 3,399,745 + Psest_3228 0.55 -0.8 3,399,745 + Psest_3228 0.55 -1.5 3,399,745 + Psest_3228 0.55 +1.6
Or see this region's nucleotide sequence