Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0291 and Psest_0292 are separated by 57 nucleotides Psest_0292 and Psest_0293 are separated by 22 nucleotides Psest_0293 and Psest_0294 overlap by 4 nucleotides
Psest_0291: Psest_0291 - aspartate carbamoyltransferase, at 306,494 to 307,510
_0291
Psest_0292: Psest_0292 - Pyrimidine operon attenuation protein/uracil phosphoribosyltransferase, at 307,568 to 308,080
_0292
Psest_0293: Psest_0293 - RNAse H-fold protein YqgF, at 308,103 to 308,531
_0293
Psest_0294: Psest_0294 - Putative transcriptional regulator, at 308,528 to 309,097
_0294
Position (kb) 307
308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.732 kb on + strand, within Psest_0291 at 306.732 kb on + strand, within Psest_0291 at 306.741 kb on + strand, within Psest_0291 at 307.482 kb on - strand at 307.545 kb on + strand at 307.545 kb on + strand at 307.557 kb on + strand at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 308.022 kb on + strand, within Psest_0292 at 308.607 kb on + strand, within Psest_0294 at 309.006 kb on + strand, within Psest_0294 at 309.006 kb on + strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294 at 309.007 kb on - strand, within Psest_0294
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 306,595 - -0.7 306,595 - -0.9 306,595 - +1.4 306,732 + Psest_0291 0.23 -0.5 306,732 + Psest_0291 0.23 +0.1 306,741 + Psest_0291 0.24 -0.2 307,482 - -1.8 307,545 + -2.3 307,545 + -0.5 307,557 + -2.1 307,667 + Psest_0292 0.19 -1.4 307,667 + Psest_0292 0.19 -3.4 307,667 + Psest_0292 0.19 -1.4 307,668 - Psest_0292 0.19 -0.2 307,668 - Psest_0292 0.19 -1.5 307,668 - Psest_0292 0.19 -0.1 308,022 + Psest_0292 0.88 -1.4 308,607 + Psest_0294 0.14 +0.7 309,006 + Psest_0294 0.84 -1.6 309,006 + Psest_0294 0.84 -0.5 309,007 - Psest_0294 0.84 -1.9 309,007 - Psest_0294 0.84 -0.8 309,007 - Psest_0294 0.84 -1.0 309,007 - Psest_0294 0.84 -0.8 309,007 - Psest_0294 0.84 -0.3 309,007 - Psest_0294 0.84 +0.1 309,007 - Psest_0294 0.84 -0.1 309,007 - Psest_0294 0.84 -1.4
Or see this region's nucleotide sequence