Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0283 and Psest_0284 are separated by 10 nucleotides Psest_0284 and Psest_0285 are separated by 66 nucleotides Psest_0285 and Psest_0286 are separated by 59 nucleotides
Psest_0283: Psest_0283 - pyrroline-5-carboxylate reductase, at 298,749 to 299,573
_0283
Psest_0284: Psest_0284 - pyridoxal phosphate enzyme, YggS family, at 299,584 to 300,273
_0284
Psest_0285: Psest_0285 - pilus retraction protein PilT, at 300,340 to 301,374
_0285
Psest_0286: Psest_0286 - pilus retraction protein PilT, at 301,434 to 302,579
_0286
Position (kb) 300
301
302 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 299.573 kb on + strand at 299.574 kb on - strand at 299.574 kb on - strand at 299.574 kb on - strand at 299.574 kb on - strand at 299.574 kb on - strand at 299.698 kb on - strand, within Psest_0284 at 299.845 kb on + strand, within Psest_0284 at 299.845 kb on + strand, within Psest_0284 at 299.846 kb on - strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.908 kb on + strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.909 kb on - strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.910 kb on + strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.911 kb on - strand, within Psest_0284 at 299.923 kb on + strand, within Psest_0284 at 299.923 kb on + strand, within Psest_0284 at 299.924 kb on - strand, within Psest_0284 at 300.101 kb on + strand, within Psest_0284 at 300.101 kb on + strand, within Psest_0284 at 300.101 kb on + strand, within Psest_0284 at 300.101 kb on + strand, within Psest_0284 at 300.101 kb on + strand, within Psest_0284 at 300.101 kb on + strand, within Psest_0284 at 300.102 kb on - strand, within Psest_0284 at 300.102 kb on - strand, within Psest_0284 at 300.102 kb on - strand, within Psest_0284 at 300.102 kb on - strand, within Psest_0284 at 300.102 kb on - strand, within Psest_0284 at 300.102 kb on - strand, within Psest_0284 at 300.252 kb on + strand at 300.252 kb on + strand at 300.253 kb on - strand at 300.253 kb on - strand at 300.253 kb on - strand at 300.253 kb on - strand at 300.274 kb on + strand at 300.274 kb on + strand at 300.274 kb on + strand at 300.274 kb on + strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.275 kb on - strand at 300.311 kb on + strand at 300.311 kb on + strand at 300.311 kb on + strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.312 kb on - strand at 300.326 kb on - strand at 300.326 kb on - strand at 300.346 kb on - strand at 300.493 kb on + strand, within Psest_0285 at 300.493 kb on + strand, within Psest_0285 at 300.493 kb on + strand, within Psest_0285 at 300.494 kb on - strand, within Psest_0285 at 300.494 kb on - strand, within Psest_0285 at 300.494 kb on - strand, within Psest_0285 at 300.494 kb on - strand, within Psest_0285 at 300.494 kb on - strand, within Psest_0285 at 300.494 kb on - strand, within Psest_0285 at 300.776 kb on - strand, within Psest_0285 at 300.793 kb on + strand, within Psest_0285 at 300.793 kb on + strand, within Psest_0285 at 300.794 kb on - strand, within Psest_0285 at 300.794 kb on - strand, within Psest_0285 at 300.794 kb on - strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.243 kb on + strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.244 kb on - strand, within Psest_0285 at 301.323 kb on + strand at 301.323 kb on + strand at 301.323 kb on + strand at 301.323 kb on + strand at 301.324 kb on - strand at 301.372 kb on + strand at 301.373 kb on - strand at 301.427 kb on + strand at 301.428 kb on - strand at 301.693 kb on - strand, within Psest_0286 at 301.693 kb on - strand, within Psest_0286 at 302.070 kb on + strand, within Psest_0286 at 302.070 kb on + strand, within Psest_0286 at 302.071 kb on - strand, within Psest_0286 at 302.071 kb on - strand, within Psest_0286 at 302.071 kb on - strand, within Psest_0286
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 299,573 + -0.2 299,574 - +1.4 299,574 - -0.9 299,574 - +1.2 299,574 - -1.9 299,574 - -0.4 299,698 - Psest_0284 0.17 -1.8 299,845 + Psest_0284 0.38 -3.0 299,845 + Psest_0284 0.38 -1.2 299,846 - Psest_0284 0.38 -1.7 299,908 + Psest_0284 0.47 -1.1 299,908 + Psest_0284 0.47 -2.4 299,908 + Psest_0284 0.47 -2.1 299,908 + Psest_0284 0.47 -1.0 299,908 + Psest_0284 0.47 -2.3 299,908 + Psest_0284 0.47 -1.4 299,908 + Psest_0284 0.47 -1.0 299,909 - Psest_0284 0.47 -2.3 299,909 - Psest_0284 0.47 -1.0 299,909 - Psest_0284 0.47 -1.2 299,909 - Psest_0284 0.47 -0.7 299,909 - Psest_0284 0.47 -1.7 299,909 - Psest_0284 0.47 -1.3 299,909 - Psest_0284 0.47 -1.2 299,909 - Psest_0284 0.47 -2.4 299,909 - Psest_0284 0.47 -0.8 299,909 - Psest_0284 0.47 -1.7 299,909 - Psest_0284 0.47 -0.6 299,909 - Psest_0284 0.47 -1.1 299,909 - Psest_0284 0.47 -1.4 299,909 - Psest_0284 0.47 -2.3 299,909 - Psest_0284 0.47 -1.6 299,909 - Psest_0284 0.47 -1.9 299,909 - Psest_0284 0.47 -1.7 299,909 - Psest_0284 0.47 -1.4 299,909 - Psest_0284 0.47 +0.4 299,909 - Psest_0284 0.47 -0.6 299,909 - Psest_0284 0.47 -0.4 299,910 + Psest_0284 0.47 -1.0 299,910 + Psest_0284 0.47 -1.6 299,910 + Psest_0284 0.47 -0.8 299,910 + Psest_0284 0.47 -0.9 299,910 + Psest_0284 0.47 -2.3 299,910 + Psest_0284 0.47 -1.1 299,910 + Psest_0284 0.47 -2.4 299,910 + Psest_0284 0.47 -1.4 299,911 - Psest_0284 0.47 -0.6 299,911 - Psest_0284 0.47 -0.2 299,911 - Psest_0284 0.47 -0.9 299,911 - Psest_0284 0.47 -0.6 299,911 - Psest_0284 0.47 -0.6 299,911 - Psest_0284 0.47 -0.6 299,911 - Psest_0284 0.47 +0.3 299,911 - Psest_0284 0.47 -1.8 299,911 - Psest_0284 0.47 -3.2 299,911 - Psest_0284 0.47 -1.8 299,911 - Psest_0284 0.47 -0.5 299,911 - Psest_0284 0.47 -1.0 299,911 - Psest_0284 0.47 -0.9 299,911 - Psest_0284 0.47 -1.6 299,911 - Psest_0284 0.47 -1.1 299,911 - Psest_0284 0.47 -1.4 299,911 - Psest_0284 0.47 -0.6 299,911 - Psest_0284 0.47 +0.5 299,911 - Psest_0284 0.47 -1.2 299,911 - Psest_0284 0.47 -2.3 299,923 + Psest_0284 0.49 -0.5 299,923 + Psest_0284 0.49 -0.8 299,924 - Psest_0284 0.49 -0.6 300,101 + Psest_0284 0.75 -0.2 300,101 + Psest_0284 0.75 -2.6 300,101 + Psest_0284 0.75 -1.1 300,101 + Psest_0284 0.75 -2.0 300,101 + Psest_0284 0.75 -1.9 300,101 + Psest_0284 0.75 -3.7 300,102 - Psest_0284 0.75 -1.2 300,102 - Psest_0284 0.75 -1.2 300,102 - Psest_0284 0.75 -0.5 300,102 - Psest_0284 0.75 -0.8 300,102 - Psest_0284 0.75 -1.2 300,102 - Psest_0284 0.75 -1.1 300,252 + -0.7 300,252 + -0.7 300,253 - +0.8 300,253 - +0.0 300,253 - +0.2 300,253 - +0.6 300,274 + +0.4 300,274 + -0.8 300,274 + -1.6 300,274 + -1.6 300,275 - -0.5 300,275 - -1.7 300,275 - -1.0 300,275 - -0.1 300,275 - -0.8 300,275 - +0.2 300,275 - -1.4 300,275 - -0.4 300,275 - -1.1 300,275 - -1.9 300,275 - -0.8 300,275 - -1.2 300,275 - +0.0 300,311 + -2.0 300,311 + +0.6 300,311 + -0.5 300,312 - -0.6 300,312 - -0.5 300,312 - -1.3 300,312 - -0.4 300,312 - -1.3 300,312 - -1.6 300,312 - -4.7 300,312 - -1.5 300,312 - -0.5 300,312 - -0.5 300,312 - -1.0 300,312 - -1.5 300,326 - -0.8 300,326 - -1.4 300,346 - -0.4 300,493 + Psest_0285 0.15 -1.6 300,493 + Psest_0285 0.15 -1.8 300,493 + Psest_0285 0.15 -1.4 300,494 - Psest_0285 0.15 -1.1 300,494 - Psest_0285 0.15 -1.3 300,494 - Psest_0285 0.15 -1.6 300,494 - Psest_0285 0.15 -1.5 300,494 - Psest_0285 0.15 -1.9 300,494 - Psest_0285 0.15 +0.4 300,776 - Psest_0285 0.42 -1.1 300,793 + Psest_0285 0.44 -2.2 300,793 + Psest_0285 0.44 -1.5 300,794 - Psest_0285 0.44 -0.3 300,794 - Psest_0285 0.44 -1.8 300,794 - Psest_0285 0.44 -3.1 301,243 + Psest_0285 0.87 -2.1 301,243 + Psest_0285 0.87 -0.8 301,243 + Psest_0285 0.87 -2.1 301,243 + Psest_0285 0.87 -0.7 301,243 + Psest_0285 0.87 -1.6 301,243 + Psest_0285 0.87 +0.1 301,243 + Psest_0285 0.87 -2.3 301,243 + Psest_0285 0.87 +0.1 301,243 + Psest_0285 0.87 -0.4 301,243 + Psest_0285 0.87 -0.5 301,244 - Psest_0285 0.87 -2.9 301,244 - Psest_0285 0.87 -1.6 301,244 - Psest_0285 0.87 -1.3 301,244 - Psest_0285 0.87 -3.3 301,244 - Psest_0285 0.87 -1.7 301,244 - Psest_0285 0.87 -2.1 301,244 - Psest_0285 0.87 -0.4 301,244 - Psest_0285 0.87 -2.0 301,244 - Psest_0285 0.87 -1.7 301,244 - Psest_0285 0.87 -1.1 301,244 - Psest_0285 0.87 -0.9 301,323 + -2.6 301,323 + -0.1 301,323 + -3.7 301,323 + -2.3 301,324 - -1.5 301,372 + +0.3 301,373 - -1.4 301,427 + -0.8 301,428 - -0.2 301,693 - Psest_0286 0.23 -0.8 301,693 - Psest_0286 0.23 -0.2 302,070 + Psest_0286 0.55 -0.2 302,070 + Psest_0286 0.55 -1.7 302,071 - Psest_0286 0.56 -1.2 302,071 - Psest_0286 0.56 -0.5 302,071 - Psest_0286 0.56 -1.7
Or see this region's nucleotide sequence