Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2601 and Psest_2602 overlap by 32 nucleotides Psest_2602 and Psest_2603 are separated by 51 nucleotides Psest_2603 and Psest_2604 are separated by 25 nucleotides Psest_2604 and Psest_2605 are separated by 57 nucleotides
Psest_2601: Psest_2601 - Membrane protein involved in the export of O-antigen and teichoic acid, at 2,782,208 to 2,783,770
_2601
Psest_2602: Psest_2602 - Glycosyltransferases involved in cell wall biogenesis, at 2,783,739 to 2,784,671
_2602
Psest_2603: Psest_2603 - capsular exopolysaccharide family, at 2,784,723 to 2,786,936
_2603
Psest_2604: Psest_2604 - WbqC-like protein family., at 2,786,962 to 2,787,705
_2604
Psest_2605: Psest_2605 - transcriptional activator RfaH, at 2,787,763 to 2,788,272
_2605
Position (kb)
2784
2785
2786
2787 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2783.820 kb on + strand at 2783.820 kb on + strand at 2783.820 kb on + strand at 2783.820 kb on + strand at 2783.820 kb on + strand at 2783.821 kb on - strand at 2783.821 kb on - strand at 2783.982 kb on + strand, within Psest_2602 at 2783.982 kb on + strand, within Psest_2602 at 2784.054 kb on + strand, within Psest_2602 at 2784.054 kb on + strand, within Psest_2602 at 2784.054 kb on + strand, within Psest_2602 at 2784.054 kb on + strand, within Psest_2602 at 2784.054 kb on + strand, within Psest_2602 at 2784.054 kb on + strand, within Psest_2602 at 2784.055 kb on - strand, within Psest_2602 at 2784.055 kb on - strand, within Psest_2602 at 2784.055 kb on - strand, within Psest_2602 at 2784.142 kb on - strand, within Psest_2602 at 2784.153 kb on + strand, within Psest_2602 at 2784.153 kb on + strand, within Psest_2602 at 2784.484 kb on - strand, within Psest_2602 at 2784.501 kb on + strand, within Psest_2602 at 2784.501 kb on + strand, within Psest_2602 at 2784.501 kb on + strand, within Psest_2602 at 2784.502 kb on - strand, within Psest_2602 at 2784.502 kb on - strand, within Psest_2602 at 2784.502 kb on - strand, within Psest_2602 at 2784.529 kb on - strand, within Psest_2602 at 2784.534 kb on + strand, within Psest_2602 at 2784.534 kb on + strand, within Psest_2602 at 2784.753 kb on + strand at 2784.753 kb on + strand at 2784.754 kb on - strand at 2784.802 kb on - strand at 2784.861 kb on + strand at 2784.861 kb on + strand at 2784.861 kb on + strand at 2784.861 kb on + strand at 2784.861 kb on + strand at 2784.862 kb on - strand at 2784.862 kb on - strand at 2784.862 kb on - strand at 2784.862 kb on - strand at 2784.862 kb on - strand at 2784.862 kb on - strand at 2784.862 kb on - strand at 2784.886 kb on - strand at 2784.886 kb on - strand at 2785.047 kb on + strand, within Psest_2603 at 2785.047 kb on + strand, within Psest_2603 at 2785.047 kb on + strand, within Psest_2603 at 2785.048 kb on - strand, within Psest_2603 at 2785.048 kb on - strand, within Psest_2603 at 2785.048 kb on - strand, within Psest_2603 at 2785.151 kb on + strand, within Psest_2603 at 2785.152 kb on - strand, within Psest_2603 at 2785.158 kb on + strand, within Psest_2603 at 2785.158 kb on + strand, within Psest_2603 at 2785.159 kb on - strand, within Psest_2603 at 2785.159 kb on - strand, within Psest_2603 at 2785.159 kb on - strand, within Psest_2603 at 2785.212 kb on + strand, within Psest_2603 at 2785.212 kb on + strand, within Psest_2603 at 2785.213 kb on - strand, within Psest_2603 at 2785.213 kb on - strand, within Psest_2603 at 2785.213 kb on - strand, within Psest_2603 at 2785.416 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.467 kb on + strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.468 kb on - strand, within Psest_2603 at 2785.510 kb on + strand, within Psest_2603 at 2785.511 kb on - strand, within Psest_2603 at 2785.511 kb on - strand, within Psest_2603 at 2785.511 kb on - strand, within Psest_2603 at 2785.533 kb on + strand, within Psest_2603 at 2785.534 kb on - strand, within Psest_2603 at 2785.534 kb on - strand, within Psest_2603 at 2785.654 kb on - strand, within Psest_2603 at 2785.654 kb on - strand, within Psest_2603 at 2785.765 kb on - strand, within Psest_2603 at 2785.776 kb on + strand, within Psest_2603 at 2785.776 kb on + strand, within Psest_2603 at 2785.776 kb on + strand, within Psest_2603 at 2785.798 kb on - strand, within Psest_2603 at 2785.849 kb on + strand, within Psest_2603 at 2785.850 kb on - strand, within Psest_2603 at 2785.850 kb on - strand, within Psest_2603 at 2785.850 kb on - strand, within Psest_2603 at 2785.932 kb on + strand, within Psest_2603 at 2785.933 kb on - strand, within Psest_2603 at 2786.016 kb on + strand, within Psest_2603 at 2786.016 kb on + strand, within Psest_2603 at 2786.127 kb on + strand, within Psest_2603 at 2786.178 kb on + strand, within Psest_2603 at 2786.179 kb on - strand, within Psest_2603 at 2786.674 kb on - strand, within Psest_2603 at 2786.910 kb on + strand at 2786.910 kb on + strand at 2786.910 kb on + strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.911 kb on - strand at 2786.916 kb on + strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.917 kb on - strand at 2786.992 kb on + strand at 2786.993 kb on - strand at 2786.996 kb on + strand at 2786.996 kb on + strand at 2786.996 kb on + strand at 2786.996 kb on + strand at 2786.996 kb on + strand at 2786.996 kb on + strand at 2786.997 kb on - strand at 2786.997 kb on - strand at 2786.997 kb on - strand at 2786.997 kb on - strand at 2786.997 kb on - strand at 2786.997 kb on - strand at 2786.997 kb on - strand at 2787.005 kb on - strand at 2787.005 kb on - strand at 2787.013 kb on + strand at 2787.067 kb on + strand, within Psest_2604 at 2787.067 kb on + strand, within Psest_2604 at 2787.068 kb on - strand, within Psest_2604 at 2787.068 kb on - strand, within Psest_2604 at 2787.068 kb on - strand, within Psest_2604 at 2787.068 kb on - strand, within Psest_2604 at 2787.068 kb on - strand, within Psest_2604 at 2787.068 kb on - strand, within Psest_2604 at 2787.239 kb on - strand, within Psest_2604 at 2787.239 kb on - strand, within Psest_2604 at 2787.352 kb on + strand, within Psest_2604 at 2787.438 kb on - strand, within Psest_2604 at 2787.464 kb on - strand, within Psest_2604 at 2787.464 kb on - strand, within Psest_2604 at 2787.464 kb on - strand, within Psest_2604 at 2787.464 kb on - strand, within Psest_2604 at 2787.494 kb on - strand, within Psest_2604 at 2787.559 kb on + strand, within Psest_2604 at 2787.559 kb on + strand, within Psest_2604 at 2787.560 kb on - strand, within Psest_2604 at 2787.560 kb on - strand, within Psest_2604 at 2787.560 kb on - strand, within Psest_2604 at 2787.771 kb on + strand at 2787.771 kb on + strand at 2787.772 kb on - strand at 2787.772 kb on - strand at 2787.772 kb on - strand at 2787.772 kb on - strand at 2787.772 kb on - strand at 2787.772 kb on - strand at 2787.791 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 2,783,820 + +1.1 2,783,820 + -0.1 2,783,820 + +0.1 2,783,820 + +0.5 2,783,820 + +0.6 2,783,821 - +1.0 2,783,821 - +0.9 2,783,982 + Psest_2602 0.26 -1.2 2,783,982 + Psest_2602 0.26 -1.2 2,784,054 + Psest_2602 0.34 -1.2 2,784,054 + Psest_2602 0.34 -0.8 2,784,054 + Psest_2602 0.34 -0.9 2,784,054 + Psest_2602 0.34 -0.5 2,784,054 + Psest_2602 0.34 -0.7 2,784,054 + Psest_2602 0.34 -1.6 2,784,055 - Psest_2602 0.34 +0.8 2,784,055 - Psest_2602 0.34 +0.7 2,784,055 - Psest_2602 0.34 +0.3 2,784,142 - Psest_2602 0.43 +0.6 2,784,153 + Psest_2602 0.44 -2.6 2,784,153 + Psest_2602 0.44 +0.4 2,784,484 - Psest_2602 0.80 +0.7 2,784,501 + Psest_2602 0.82 -1.8 2,784,501 + Psest_2602 0.82 -1.4 2,784,501 + Psest_2602 0.82 -2.5 2,784,502 - Psest_2602 0.82 +0.5 2,784,502 - Psest_2602 0.82 +0.9 2,784,502 - Psest_2602 0.82 +0.6 2,784,529 - Psest_2602 0.85 +1.1 2,784,534 + Psest_2602 0.85 -1.5 2,784,534 + Psest_2602 0.85 -2.2 2,784,753 + -0.6 2,784,753 + -1.3 2,784,754 - +2.0 2,784,802 - +0.8 2,784,861 + -0.8 2,784,861 + -1.1 2,784,861 + -0.2 2,784,861 + +0.1 2,784,861 + -0.7 2,784,862 - +0.4 2,784,862 - +1.2 2,784,862 - +0.4 2,784,862 - +1.5 2,784,862 - +1.0 2,784,862 - +0.9 2,784,862 - +0.2 2,784,886 - +1.2 2,784,886 - -0.3 2,785,047 + Psest_2603 0.15 +0.0 2,785,047 + Psest_2603 0.15 +0.2 2,785,047 + Psest_2603 0.15 -0.2 2,785,048 - Psest_2603 0.15 +0.9 2,785,048 - Psest_2603 0.15 +0.8 2,785,048 - Psest_2603 0.15 +2.1 2,785,151 + Psest_2603 0.19 +0.4 2,785,152 - Psest_2603 0.19 +1.8 2,785,158 + Psest_2603 0.20 -0.2 2,785,158 + Psest_2603 0.20 +0.4 2,785,159 - Psest_2603 0.20 +1.5 2,785,159 - Psest_2603 0.20 +2.1 2,785,159 - Psest_2603 0.20 -0.2 2,785,212 + Psest_2603 0.22 +1.0 2,785,212 + Psest_2603 0.22 +1.3 2,785,213 - Psest_2603 0.22 +1.4 2,785,213 - Psest_2603 0.22 +0.6 2,785,213 - Psest_2603 0.22 +1.0 2,785,416 + Psest_2603 0.31 +0.6 2,785,467 + Psest_2603 0.34 +1.3 2,785,467 + Psest_2603 0.34 +0.5 2,785,467 + Psest_2603 0.34 +0.2 2,785,467 + Psest_2603 0.34 +0.7 2,785,467 + Psest_2603 0.34 +0.9 2,785,467 + Psest_2603 0.34 +0.3 2,785,467 + Psest_2603 0.34 -1.4 2,785,467 + Psest_2603 0.34 -0.4 2,785,467 + Psest_2603 0.34 -0.5 2,785,467 + Psest_2603 0.34 -0.1 2,785,467 + Psest_2603 0.34 -1.5 2,785,467 + Psest_2603 0.34 -1.0 2,785,468 - Psest_2603 0.34 +1.2 2,785,468 - Psest_2603 0.34 +1.2 2,785,468 - Psest_2603 0.34 +1.0 2,785,468 - Psest_2603 0.34 +0.9 2,785,468 - Psest_2603 0.34 +0.6 2,785,468 - Psest_2603 0.34 +1.9 2,785,468 - Psest_2603 0.34 +0.8 2,785,468 - Psest_2603 0.34 +0.9 2,785,468 - Psest_2603 0.34 +1.7 2,785,468 - Psest_2603 0.34 +1.2 2,785,468 - Psest_2603 0.34 +1.1 2,785,468 - Psest_2603 0.34 +0.9 2,785,468 - Psest_2603 0.34 +1.1 2,785,468 - Psest_2603 0.34 +1.1 2,785,468 - Psest_2603 0.34 +0.5 2,785,468 - Psest_2603 0.34 +1.0 2,785,468 - Psest_2603 0.34 +0.6 2,785,468 - Psest_2603 0.34 +1.3 2,785,468 - Psest_2603 0.34 +0.5 2,785,468 - Psest_2603 0.34 +1.3 2,785,468 - Psest_2603 0.34 +0.2 2,785,468 - Psest_2603 0.34 +0.9 2,785,468 - Psest_2603 0.34 +0.6 2,785,468 - Psest_2603 0.34 +1.3 2,785,468 - Psest_2603 0.34 +0.7 2,785,510 + Psest_2603 0.36 -2.0 2,785,511 - Psest_2603 0.36 +0.5 2,785,511 - Psest_2603 0.36 +1.1 2,785,511 - Psest_2603 0.36 +0.4 2,785,533 + Psest_2603 0.37 -0.6 2,785,534 - Psest_2603 0.37 +0.8 2,785,534 - Psest_2603 0.37 +0.4 2,785,654 - Psest_2603 0.42 +0.6 2,785,654 - Psest_2603 0.42 +1.0 2,785,765 - Psest_2603 0.47 +0.4 2,785,776 + Psest_2603 0.48 -0.4 2,785,776 + Psest_2603 0.48 +0.4 2,785,776 + Psest_2603 0.48 +0.2 2,785,798 - Psest_2603 0.49 +1.1 2,785,849 + Psest_2603 0.51 -0.5 2,785,850 - Psest_2603 0.51 +0.6 2,785,850 - Psest_2603 0.51 +0.5 2,785,850 - Psest_2603 0.51 +0.5 2,785,932 + Psest_2603 0.55 -0.3 2,785,933 - Psest_2603 0.55 +1.6 2,786,016 + Psest_2603 0.58 -2.0 2,786,016 + Psest_2603 0.58 -0.6 2,786,127 + Psest_2603 0.63 +0.4 2,786,178 + Psest_2603 0.66 +0.6 2,786,179 - Psest_2603 0.66 +0.5 2,786,674 - Psest_2603 0.88 +1.1 2,786,910 + -2.3 2,786,910 + -1.9 2,786,910 + -2.2 2,786,911 - +0.5 2,786,911 - +0.1 2,786,911 - +1.8 2,786,911 - +0.9 2,786,911 - +0.9 2,786,911 - +1.0 2,786,911 - +1.1 2,786,911 - +0.5 2,786,916 + -0.6 2,786,917 - +2.1 2,786,917 - +0.3 2,786,917 - +0.7 2,786,917 - -0.7 2,786,917 - +0.5 2,786,917 - +0.6 2,786,917 - +1.0 2,786,917 - +2.8 2,786,917 - +0.8 2,786,917 - +1.8 2,786,917 - +0.2 2,786,917 - +1.0 2,786,917 - +1.0 2,786,917 - +1.2 2,786,992 + +0.3 2,786,993 - +0.9 2,786,996 + -0.6 2,786,996 + -0.8 2,786,996 + +0.6 2,786,996 + -1.2 2,786,996 + -1.3 2,786,996 + -0.3 2,786,997 - +0.8 2,786,997 - +1.2 2,786,997 - +1.5 2,786,997 - +0.8 2,786,997 - +1.2 2,786,997 - +0.5 2,786,997 - +1.1 2,787,005 - +0.8 2,787,005 - +1.7 2,787,013 + -0.5 2,787,067 + Psest_2604 0.14 -1.0 2,787,067 + Psest_2604 0.14 -0.1 2,787,068 - Psest_2604 0.14 +0.6 2,787,068 - Psest_2604 0.14 +0.9 2,787,068 - Psest_2604 0.14 +1.9 2,787,068 - Psest_2604 0.14 +0.8 2,787,068 - Psest_2604 0.14 -0.7 2,787,068 - Psest_2604 0.14 +0.2 2,787,239 - Psest_2604 0.37 +1.0 2,787,239 - Psest_2604 0.37 +1.2 2,787,352 + Psest_2604 0.52 -0.7 2,787,438 - Psest_2604 0.64 +1.0 2,787,464 - Psest_2604 0.67 -1.5 2,787,464 - Psest_2604 0.67 +1.2 2,787,464 - Psest_2604 0.67 +0.7 2,787,464 - Psest_2604 0.67 +1.5 2,787,494 - Psest_2604 0.72 +1.4 2,787,559 + Psest_2604 0.80 -1.2 2,787,559 + Psest_2604 0.80 -3.5 2,787,560 - Psest_2604 0.80 +1.3 2,787,560 - Psest_2604 0.80 +1.1 2,787,560 - Psest_2604 0.80 +1.1 2,787,771 + -0.7 2,787,771 + +0.3 2,787,772 - +1.2 2,787,772 - +0.6 2,787,772 - +0.6 2,787,772 - +1.4 2,787,772 - +1.3 2,787,772 - +1.1 2,787,791 - +1.5
Or see this region's nucleotide sequence