Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2521 and Psest_2522 are separated by 379 nucleotides Psest_2522 and Psest_2523 are separated by 48 nucleotides Psest_2523 and Psest_2524 are separated by 63 nucleotides
Psest_2521: Psest_2521 - aconitate hydratase 1, at 2,699,028 to 2,701,703
_2521
Psest_2522: Psest_2522 - PAS domain S-box, at 2,702,083 to 2,703,651
_2522
Psest_2523: Psest_2523 - Predicted hydrolase of the alpha/beta-hydrolase fold, at 2,703,700 to 2,704,392
_2523
Psest_2524: Psest_2524 - cytochrome c oxidase, cbb3-type, subunit I, at 2,704,456 to 2,705,880
_2524
Position (kb)
2702
2703
2704 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2701.095 kb on + strand, within Psest_2521 at 2701.095 kb on + strand, within Psest_2521 at 2701.242 kb on + strand, within Psest_2521 at 2701.242 kb on + strand, within Psest_2521 at 2701.242 kb on + strand, within Psest_2521 at 2701.243 kb on - strand, within Psest_2521 at 2701.243 kb on - strand, within Psest_2521 at 2701.243 kb on - strand, within Psest_2521 at 2701.272 kb on + strand, within Psest_2521 at 2701.272 kb on + strand, within Psest_2521 at 2701.272 kb on + strand, within Psest_2521 at 2701.272 kb on + strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.273 kb on - strand, within Psest_2521 at 2701.335 kb on + strand, within Psest_2521 at 2701.385 kb on - strand, within Psest_2521 at 2701.628 kb on + strand at 2701.647 kb on + strand at 2701.648 kb on - strand at 2701.648 kb on - strand at 2701.648 kb on - strand at 2701.674 kb on + strand at 2701.674 kb on + strand at 2701.674 kb on + strand at 2701.675 kb on - strand at 2701.675 kb on - strand at 2701.860 kb on + strand at 2702.006 kb on + strand at 2702.033 kb on + strand at 2702.034 kb on - strand at 2702.034 kb on - strand at 2702.055 kb on - strand at 2702.055 kb on - strand at 2702.055 kb on - strand at 2702.060 kb on + strand at 2702.060 kb on + strand at 2702.060 kb on + strand at 2702.061 kb on - strand at 2702.116 kb on + strand at 2702.117 kb on - strand at 2702.117 kb on - strand at 2702.142 kb on - strand at 2702.176 kb on + strand at 2702.176 kb on + strand at 2702.177 kb on - strand at 2702.177 kb on - strand at 2702.177 kb on - strand at 2702.196 kb on - strand at 2702.229 kb on - strand at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.286 kb on + strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.287 kb on - strand, within Psest_2522 at 2702.374 kb on + strand, within Psest_2522 at 2702.374 kb on + strand, within Psest_2522 at 2702.374 kb on + strand, within Psest_2522 at 2702.374 kb on + strand, within Psest_2522 at 2702.374 kb on + strand, within Psest_2522 at 2702.375 kb on - strand, within Psest_2522 at 2702.375 kb on - strand, within Psest_2522 at 2702.375 kb on - strand, within Psest_2522 at 2702.417 kb on - strand, within Psest_2522 at 2702.473 kb on + strand, within Psest_2522 at 2702.474 kb on - strand, within Psest_2522 at 2702.474 kb on - strand, within Psest_2522 at 2702.474 kb on - strand, within Psest_2522 at 2702.474 kb on - strand, within Psest_2522 at 2702.474 kb on - strand, within Psest_2522 at 2702.737 kb on + strand, within Psest_2522 at 2702.737 kb on + strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.738 kb on - strand, within Psest_2522 at 2702.756 kb on + strand, within Psest_2522 at 2702.757 kb on - strand, within Psest_2522 at 2702.837 kb on + strand, within Psest_2522 at 2702.837 kb on + strand, within Psest_2522 at 2702.838 kb on - strand, within Psest_2522 at 2702.969 kb on + strand, within Psest_2522 at 2702.969 kb on + strand, within Psest_2522 at 2703.264 kb on - strand, within Psest_2522 at 2703.431 kb on + strand, within Psest_2522 at 2703.432 kb on - strand, within Psest_2522 at 2703.622 kb on + strand at 2703.852 kb on - strand, within Psest_2523 at 2703.968 kb on + strand, within Psest_2523 at 2703.969 kb on - strand, within Psest_2523 at 2703.969 kb on - strand, within Psest_2523 at 2704.178 kb on + strand, within Psest_2523 at 2704.178 kb on + strand, within Psest_2523 at 2704.178 kb on + strand, within Psest_2523 at 2704.178 kb on + strand, within Psest_2523 at 2704.178 kb on + strand, within Psest_2523 at 2704.178 kb on + strand, within Psest_2523 at 2704.408 kb on + strand at 2704.409 kb on - strand at 2704.409 kb on - strand at 2704.409 kb on - strand at 2704.411 kb on + strand at 2704.453 kb on + strand at 2704.453 kb on + strand at 2704.453 kb on + strand at 2704.453 kb on + strand at 2704.454 kb on - strand at 2704.480 kb on + strand at 2704.486 kb on + strand at 2704.486 kb on + strand at 2704.486 kb on + strand at 2704.486 kb on + strand at 2704.486 kb on + strand at 2704.486 kb on + strand at 2704.487 kb on - strand at 2704.487 kb on - strand at 2704.487 kb on - strand at 2704.487 kb on - strand at 2704.487 kb on - strand at 2704.622 kb on + strand, within Psest_2524 at 2704.623 kb on - strand, within Psest_2524
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 2,701,095 + Psest_2521 0.77 -1.1 2,701,095 + Psest_2521 0.77 +0.5 2,701,242 + Psest_2521 0.83 +0.3 2,701,242 + Psest_2521 0.83 +0.1 2,701,242 + Psest_2521 0.83 -0.3 2,701,243 - Psest_2521 0.83 +0.1 2,701,243 - Psest_2521 0.83 -1.3 2,701,243 - Psest_2521 0.83 -0.2 2,701,272 + Psest_2521 0.84 +0.3 2,701,272 + Psest_2521 0.84 +0.7 2,701,272 + Psest_2521 0.84 +0.5 2,701,272 + Psest_2521 0.84 +0.5 2,701,273 - Psest_2521 0.84 -0.9 2,701,273 - Psest_2521 0.84 +0.7 2,701,273 - Psest_2521 0.84 +0.3 2,701,273 - Psest_2521 0.84 +0.8 2,701,273 - Psest_2521 0.84 -2.7 2,701,273 - Psest_2521 0.84 -0.5 2,701,273 - Psest_2521 0.84 +0.2 2,701,273 - Psest_2521 0.84 -0.1 2,701,335 + Psest_2521 0.86 +0.7 2,701,385 - Psest_2521 0.88 +1.1 2,701,628 + +1.6 2,701,647 + +1.6 2,701,648 - -0.6 2,701,648 - -1.4 2,701,648 - -0.4 2,701,674 + -0.3 2,701,674 + +0.8 2,701,674 + +1.1 2,701,675 - -2.2 2,701,675 - -1.5 2,701,860 + -0.7 2,702,006 + -0.3 2,702,033 + -1.4 2,702,034 - +1.4 2,702,034 - +0.8 2,702,055 - +0.3 2,702,055 - +0.1 2,702,055 - -0.1 2,702,060 + -0.3 2,702,060 + -0.9 2,702,060 + +0.3 2,702,061 - +0.1 2,702,116 + +0.3 2,702,117 - +1.2 2,702,117 - -0.6 2,702,142 - -0.7 2,702,176 + -0.2 2,702,176 + -0.2 2,702,177 - -0.9 2,702,177 - +0.3 2,702,177 - +0.3 2,702,196 - -1.0 2,702,229 - -0.3 2,702,286 + Psest_2522 0.13 +0.9 2,702,286 + Psest_2522 0.13 -0.4 2,702,286 + Psest_2522 0.13 +1.2 2,702,286 + Psest_2522 0.13 +0.3 2,702,286 + Psest_2522 0.13 +0.0 2,702,286 + Psest_2522 0.13 -0.4 2,702,286 + Psest_2522 0.13 +0.6 2,702,286 + Psest_2522 0.13 -0.6 2,702,287 - Psest_2522 0.13 -0.5 2,702,287 - Psest_2522 0.13 -0.6 2,702,287 - Psest_2522 0.13 -0.4 2,702,287 - Psest_2522 0.13 -0.4 2,702,287 - Psest_2522 0.13 -0.4 2,702,287 - Psest_2522 0.13 +1.7 2,702,287 - Psest_2522 0.13 +1.2 2,702,287 - Psest_2522 0.13 -0.6 2,702,287 - Psest_2522 0.13 -0.6 2,702,287 - Psest_2522 0.13 -0.3 2,702,374 + Psest_2522 0.19 -0.3 2,702,374 + Psest_2522 0.19 +0.0 2,702,374 + Psest_2522 0.19 -1.3 2,702,374 + Psest_2522 0.19 +0.1 2,702,374 + Psest_2522 0.19 +0.0 2,702,375 - Psest_2522 0.19 -0.4 2,702,375 - Psest_2522 0.19 -0.4 2,702,375 - Psest_2522 0.19 +0.1 2,702,417 - Psest_2522 0.21 -1.1 2,702,473 + Psest_2522 0.25 -0.8 2,702,474 - Psest_2522 0.25 -0.8 2,702,474 - Psest_2522 0.25 -0.3 2,702,474 - Psest_2522 0.25 +1.1 2,702,474 - Psest_2522 0.25 -1.5 2,702,474 - Psest_2522 0.25 -0.1 2,702,737 + Psest_2522 0.42 +0.3 2,702,737 + Psest_2522 0.42 +0.0 2,702,738 - Psest_2522 0.42 -0.5 2,702,738 - Psest_2522 0.42 -1.0 2,702,738 - Psest_2522 0.42 -0.2 2,702,738 - Psest_2522 0.42 -0.2 2,702,738 - Psest_2522 0.42 -0.4 2,702,738 - Psest_2522 0.42 -0.2 2,702,738 - Psest_2522 0.42 +0.8 2,702,756 + Psest_2522 0.43 -0.1 2,702,757 - Psest_2522 0.43 +1.1 2,702,837 + Psest_2522 0.48 +0.5 2,702,837 + Psest_2522 0.48 -0.1 2,702,838 - Psest_2522 0.48 -0.2 2,702,969 + Psest_2522 0.56 +0.1 2,702,969 + Psest_2522 0.56 +0.0 2,703,264 - Psest_2522 0.75 -0.5 2,703,431 + Psest_2522 0.86 +0.7 2,703,432 - Psest_2522 0.86 -0.5 2,703,622 + +1.0 2,703,852 - Psest_2523 0.22 +0.3 2,703,968 + Psest_2523 0.39 +1.1 2,703,969 - Psest_2523 0.39 +0.5 2,703,969 - Psest_2523 0.39 +0.6 2,704,178 + Psest_2523 0.69 -0.2 2,704,178 + Psest_2523 0.69 -0.1 2,704,178 + Psest_2523 0.69 -0.3 2,704,178 + Psest_2523 0.69 +0.2 2,704,178 + Psest_2523 0.69 +0.1 2,704,178 + Psest_2523 0.69 -0.2 2,704,408 + +0.2 2,704,409 - +1.3 2,704,409 - +0.3 2,704,409 - +0.4 2,704,411 + -0.1 2,704,453 + +0.7 2,704,453 + -2.0 2,704,453 + +2.1 2,704,453 + +0.8 2,704,454 - +0.2 2,704,480 + +0.2 2,704,486 + -0.9 2,704,486 + +0.6 2,704,486 + -0.0 2,704,486 + +0.3 2,704,486 + +1.3 2,704,486 + -0.3 2,704,487 - +0.7 2,704,487 - +3.2 2,704,487 - +0.3 2,704,487 - -1.7 2,704,487 - -0.5 2,704,622 + Psest_2524 0.12 -0.9 2,704,623 - Psest_2524 0.12 -0.5
Or see this region's nucleotide sequence