Experiment: Def Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2433 and Psest_2434 are separated by 65 nucleotides Psest_2434 and Psest_2435 are separated by 121 nucleotides Psest_2435 and Psest_2436 are separated by 156 nucleotides
Psest_2433: Psest_2433 - alpha-glucan phosphorylases, at 2,599,835 to 2,602,345
_2433
Psest_2434: Psest_2434 - Protein of unknown function (DUF2892)., at 2,602,411 to 2,602,764
_2434
Psest_2435: Psest_2435 - Transcriptional regulator, at 2,602,886 to 2,603,776
_2435
Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 2,603,933 to 2,605,444
_2436
Position (kb)
2602
2603
2604 Strain fitness (log2 ratio)
-2
-1
0
1 at 2601.923 kb on + strand, within Psest_2433 at 2601.923 kb on + strand, within Psest_2433 at 2601.938 kb on + strand, within Psest_2433 at 2601.938 kb on + strand, within Psest_2433 at 2602.031 kb on + strand, within Psest_2433 at 2602.031 kb on + strand, within Psest_2433 at 2602.242 kb on + strand at 2602.243 kb on - strand at 2602.265 kb on + strand at 2602.266 kb on - strand at 2602.266 kb on - strand at 2602.380 kb on + strand at 2602.380 kb on + strand at 2602.380 kb on + strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.381 kb on - strand at 2602.382 kb on + strand at 2602.382 kb on + strand at 2602.395 kb on + strand at 2602.395 kb on + strand at 2602.494 kb on - strand, within Psest_2434 at 2602.656 kb on + strand, within Psest_2434 at 2602.656 kb on + strand, within Psest_2434 at 2602.656 kb on + strand, within Psest_2434 at 2602.884 kb on - strand at 2602.887 kb on + strand at 2602.887 kb on + strand at 2602.888 kb on - strand at 2602.888 kb on - strand at 2602.888 kb on - strand at 2602.936 kb on - strand at 2603.205 kb on + strand, within Psest_2435 at 2603.205 kb on + strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.206 kb on - strand, within Psest_2435 at 2603.268 kb on + strand, within Psest_2435 at 2603.268 kb on + strand, within Psest_2435 at 2603.269 kb on - strand, within Psest_2435 at 2603.283 kb on + strand, within Psest_2435 at 2603.283 kb on + strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.284 kb on - strand, within Psest_2435 at 2603.614 kb on - strand, within Psest_2435 at 2603.754 kb on + strand at 2603.754 kb on + strand at 2603.755 kb on - strand at 2603.839 kb on + strand at 2603.861 kb on - strand at 2603.933 kb on - strand at 2603.933 kb on - strand at 2603.933 kb on - strand at 2603.956 kb on + strand at 2604.164 kb on + strand, within Psest_2436 at 2604.164 kb on + strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.165 kb on - strand, within Psest_2436 at 2604.350 kb on + strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.351 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436 at 2604.651 kb on - strand, within Psest_2436
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.0001 mg/ml remove 2,601,923 + Psest_2433 0.83 +0.4 2,601,923 + Psest_2433 0.83 -0.1 2,601,938 + Psest_2433 0.84 -0.3 2,601,938 + Psest_2433 0.84 -0.1 2,602,031 + Psest_2433 0.87 -1.3 2,602,031 + Psest_2433 0.87 +0.2 2,602,242 + +0.2 2,602,243 - +0.7 2,602,265 + -0.4 2,602,266 - -0.6 2,602,266 - -0.3 2,602,380 + -0.8 2,602,380 + -1.1 2,602,380 + -0.2 2,602,381 - +0.7 2,602,381 - +0.7 2,602,381 - +0.4 2,602,381 - -0.5 2,602,381 - -0.2 2,602,381 - -0.7 2,602,381 - +0.0 2,602,381 - -0.5 2,602,381 - -0.4 2,602,381 - +1.4 2,602,381 - -0.5 2,602,381 - +0.8 2,602,381 - +0.2 2,602,381 - -0.2 2,602,381 - +0.6 2,602,381 - +0.0 2,602,381 - -1.1 2,602,381 - +0.0 2,602,382 + -1.1 2,602,382 + +0.0 2,602,395 + +0.1 2,602,395 + +0.3 2,602,494 - Psest_2434 0.23 -0.5 2,602,656 + Psest_2434 0.69 +0.1 2,602,656 + Psest_2434 0.69 -0.4 2,602,656 + Psest_2434 0.69 +0.9 2,602,884 - -1.3 2,602,887 + +0.4 2,602,887 + -0.2 2,602,888 - +0.2 2,602,888 - +0.5 2,602,888 - -0.0 2,602,936 - +0.5 2,603,205 + Psest_2435 0.36 +0.4 2,603,205 + Psest_2435 0.36 +0.6 2,603,206 - Psest_2435 0.36 -0.6 2,603,206 - Psest_2435 0.36 -1.4 2,603,206 - Psest_2435 0.36 -0.6 2,603,268 + Psest_2435 0.43 -1.3 2,603,268 + Psest_2435 0.43 -0.9 2,603,269 - Psest_2435 0.43 -0.7 2,603,283 + Psest_2435 0.45 -2.2 2,603,283 + Psest_2435 0.45 -0.5 2,603,284 - Psest_2435 0.45 -1.9 2,603,284 - Psest_2435 0.45 -0.8 2,603,284 - Psest_2435 0.45 -0.6 2,603,284 - Psest_2435 0.45 -1.2 2,603,614 - Psest_2435 0.82 -0.7 2,603,754 + -1.7 2,603,754 + -0.8 2,603,755 - -0.3 2,603,839 + -0.7 2,603,861 - -0.3 2,603,933 - -0.3 2,603,933 - +1.0 2,603,933 - +0.0 2,603,956 + -1.2 2,604,164 + Psest_2436 0.15 -0.1 2,604,164 + Psest_2436 0.15 +0.4 2,604,165 - Psest_2436 0.15 +0.4 2,604,165 - Psest_2436 0.15 -0.1 2,604,165 - Psest_2436 0.15 -0.8 2,604,165 - Psest_2436 0.15 +0.3 2,604,350 + Psest_2436 0.28 -0.4 2,604,351 - Psest_2436 0.28 +0.3 2,604,351 - Psest_2436 0.28 +0.0 2,604,351 - Psest_2436 0.28 +0.3 2,604,351 - Psest_2436 0.28 +0.4 2,604,351 - Psest_2436 0.28 +0.2 2,604,351 - Psest_2436 0.28 -0.2 2,604,351 - Psest_2436 0.28 +0.5 2,604,651 - Psest_2436 0.47 -0.1 2,604,651 - Psest_2436 0.47 +1.6
Or see this region's nucleotide sequence