Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4051 and Psest_4052 are separated by 20 nucleotides Psest_4052 and Psest_4053 overlap by 4 nucleotides Psest_4053 and Psest_4054 are separated by 115 nucleotides
Psest_4051: Psest_4051 - molybdenum-pterin binding domain, at 4,224,947 to 4,225,162
_4051
Psest_4052: Psest_4052 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 4,225,183 to 4,225,983
_4052
Psest_4053: Psest_4053 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 4,225,980 to 4,226,764
_4053
Psest_4054: Psest_4054 - alkanesulfonate monooxygenase, FMNH(2)-dependent, at 4,226,880 to 4,228,028
_4054
Position (kb)
4225
4226
4227 Strain fitness (log2 ratio)
-1
0
1 at 4225.073 kb on + strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.184 kb on + strand at 4225.270 kb on + strand, within Psest_4052 at 4225.270 kb on + strand, within Psest_4052 at 4225.372 kb on + strand, within Psest_4052 at 4225.474 kb on + strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.538 kb on - strand, within Psest_4052 at 4225.538 kb on - strand, within Psest_4052 at 4225.904 kb on - strand at 4225.941 kb on + strand at 4225.942 kb on - strand at 4225.942 kb on - strand at 4226.005 kb on + strand at 4226.006 kb on - strand at 4226.342 kb on - strand, within Psest_4053 at 4226.342 kb on - strand, within Psest_4053 at 4226.342 kb on - strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.781 kb on - strand at 4226.800 kb on + strand at 4226.801 kb on - strand at 4226.878 kb on - strand at 4226.945 kb on - strand at 4227.073 kb on + strand, within Psest_4054 at 4227.074 kb on - strand, within Psest_4054 at 4227.448 kb on + strand, within Psest_4054 at 4227.448 kb on + strand, within Psest_4054 at 4227.448 kb on + strand, within Psest_4054 at 4227.449 kb on - strand, within Psest_4054 at 4227.449 kb on - strand, within Psest_4054 at 4227.505 kb on + strand, within Psest_4054 at 4227.581 kb on - strand, within Psest_4054 at 4227.581 kb on - strand, within Psest_4054
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 4,225,073 + Psest_4051 0.58 +0.0 4,225,074 - Psest_4051 0.59 -0.5 4,225,074 - Psest_4051 0.59 +0.2 4,225,074 - Psest_4051 0.59 -0.4 4,225,074 - Psest_4051 0.59 -0.9 4,225,184 + -0.8 4,225,270 + Psest_4052 0.11 +0.1 4,225,270 + Psest_4052 0.11 +0.2 4,225,372 + Psest_4052 0.24 -0.1 4,225,474 + Psest_4052 0.36 +0.3 4,225,475 - Psest_4052 0.36 +0.3 4,225,475 - Psest_4052 0.36 +0.3 4,225,475 - Psest_4052 0.36 -0.2 4,225,475 - Psest_4052 0.36 +0.5 4,225,538 - Psest_4052 0.44 +1.7 4,225,538 - Psest_4052 0.44 +1.0 4,225,904 - +1.3 4,225,941 + +1.5 4,225,942 - +0.6 4,225,942 - +1.2 4,226,005 + +0.2 4,226,006 - +0.7 4,226,342 - Psest_4053 0.46 +0.6 4,226,342 - Psest_4053 0.46 +0.5 4,226,342 - Psest_4053 0.46 -0.4 4,226,353 + Psest_4053 0.48 -0.0 4,226,353 + Psest_4053 0.48 +1.6 4,226,353 + Psest_4053 0.48 +1.1 4,226,353 + Psest_4053 0.48 +0.7 4,226,353 + Psest_4053 0.48 +0.5 4,226,354 - Psest_4053 0.48 +0.5 4,226,354 - Psest_4053 0.48 -0.4 4,226,354 - Psest_4053 0.48 -0.8 4,226,354 - Psest_4053 0.48 -0.3 4,226,354 - Psest_4053 0.48 -0.8 4,226,354 - Psest_4053 0.48 -1.7 4,226,781 - +0.4 4,226,800 + +0.1 4,226,801 - -0.6 4,226,878 - +1.0 4,226,945 - +1.2 4,227,073 + Psest_4054 0.17 -0.3 4,227,074 - Psest_4054 0.17 -0.7 4,227,448 + Psest_4054 0.49 -0.5 4,227,448 + Psest_4054 0.49 +0.9 4,227,448 + Psest_4054 0.49 +0.7 4,227,449 - Psest_4054 0.50 +0.7 4,227,449 - Psest_4054 0.50 -0.4 4,227,505 + Psest_4054 0.54 +1.0 4,227,581 - Psest_4054 0.61 +0.7 4,227,581 - Psest_4054 0.61 +0.7
Or see this region's nucleotide sequence