Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0034

Experiment: Def Polymyxin B sulfate 0.00008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0032 and Psest_0033 are separated by 149 nucleotidesPsest_0033 and Psest_0034 are separated by 125 nucleotidesPsest_0034 and Psest_0035 are separated by 5 nucleotides Psest_0032: Psest_0032 - D-alanyl-D-alanine carboxypeptidase, at 31,732 to 32,670 _0032 Psest_0033: Psest_0033 - Protein of unknown function (DUF2946)., at 32,820 to 33,233 _0033 Psest_0034: Psest_0034 - Outer membrane receptor proteins, mostly Fe transport, at 33,359 to 35,407 _0034 Psest_0035: Psest_0035 - hypothetical protein, at 35,413 to 36,939 _0035 Position (kb) 33 34 35 36Strain fitness (log2 ratio) -1 0 1 2 3at 32.454 kb on + strand, within Psest_0032at 32.454 kb on + strand, within Psest_0032at 32.455 kb on - strand, within Psest_0032at 32.455 kb on - strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.506 kb on + strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.507 kb on - strand, within Psest_0032at 32.514 kb on + strand, within Psest_0032at 32.590 kb on + strandat 32.591 kb on - strandat 32.591 kb on - strandat 32.591 kb on - strandat 32.736 kb on + strandat 32.778 kb on + strandat 33.259 kb on + strandat 33.259 kb on + strandat 33.434 kb on + strandat 33.434 kb on + strandat 33.435 kb on - strandat 33.435 kb on - strandat 33.593 kb on + strand, within Psest_0034at 33.593 kb on + strand, within Psest_0034at 33.594 kb on - strand, within Psest_0034at 33.764 kb on + strand, within Psest_0034at 33.842 kb on + strand, within Psest_0034at 33.842 kb on + strand, within Psest_0034at 33.843 kb on - strand, within Psest_0034at 33.843 kb on - strand, within Psest_0034at 33.843 kb on - strand, within Psest_0034at 33.843 kb on - strand, within Psest_0034at 33.890 kb on + strand, within Psest_0034at 33.920 kb on + strand, within Psest_0034at 33.920 kb on + strand, within Psest_0034at 33.921 kb on - strand, within Psest_0034at 33.921 kb on - strand, within Psest_0034at 33.921 kb on - strand, within Psest_0034at 33.980 kb on + strand, within Psest_0034at 33.980 kb on + strand, within Psest_0034at 33.980 kb on + strand, within Psest_0034at 33.980 kb on + strand, within Psest_0034at 33.980 kb on + strand, within Psest_0034at 33.981 kb on - strand, within Psest_0034at 33.981 kb on - strand, within Psest_0034at 33.981 kb on - strand, within Psest_0034at 34.061 kb on + strand, within Psest_0034at 34.061 kb on + strand, within Psest_0034at 34.061 kb on + strand, within Psest_0034at 34.062 kb on - strand, within Psest_0034at 34.079 kb on - strand, within Psest_0034at 34.109 kb on + strand, within Psest_0034at 34.110 kb on - strand, within Psest_0034at 34.136 kb on + strand, within Psest_0034at 34.179 kb on - strand, within Psest_0034at 34.346 kb on - strand, within Psest_0034at 34.364 kb on + strand, within Psest_0034at 34.413 kb on - strand, within Psest_0034at 34.413 kb on - strand, within Psest_0034at 34.527 kb on - strand, within Psest_0034at 34.527 kb on - strand, within Psest_0034at 34.535 kb on + strand, within Psest_0034at 34.536 kb on - strand, within Psest_0034at 34.544 kb on + strand, within Psest_0034at 34.545 kb on - strand, within Psest_0034at 34.649 kb on + strand, within Psest_0034at 34.700 kb on + strand, within Psest_0034at 34.700 kb on + strand, within Psest_0034at 34.700 kb on + strand, within Psest_0034at 34.700 kb on + strand, within Psest_0034at 34.701 kb on - strand, within Psest_0034at 34.701 kb on - strand, within Psest_0034at 34.701 kb on - strand, within Psest_0034at 34.701 kb on - strand, within Psest_0034at 34.701 kb on - strand, within Psest_0034at 34.709 kb on - strand, within Psest_0034at 34.727 kb on + strand, within Psest_0034at 34.775 kb on + strand, within Psest_0034at 34.776 kb on - strand, within Psest_0034at 35.118 kb on - strand, within Psest_0034at 35.118 kb on - strand, within Psest_0034at 35.135 kb on + strand, within Psest_0034at 35.136 kb on - strand, within Psest_0034at 35.136 kb on - strand, within Psest_0034at 35.189 kb on + strand, within Psest_0034at 35.190 kb on - strand, within Psest_0034at 35.192 kb on + strand, within Psest_0034at 35.193 kb on - strand, within Psest_0034at 35.193 kb on - strand, within Psest_0034at 35.222 kb on + strandat 35.223 kb on - strandat 35.223 kb on - strandat 35.232 kb on - strandat 35.259 kb on - strandat 35.318 kb on + strandat 35.318 kb on + strandat 35.318 kb on + strandat 35.318 kb on + strandat 35.319 kb on - strandat 35.324 kb on + strandat 35.324 kb on + strandat 35.324 kb on + strandat 35.325 kb on - strandat 35.325 kb on - strandat 35.325 kb on - strandat 35.325 kb on - strandat 35.325 kb on - strandat 35.325 kb on - strandat 35.325 kb on - strandat 35.381 kb on + strandat 35.381 kb on + strandat 35.381 kb on + strandat 35.381 kb on + strandat 35.382 kb on - strandat 35.382 kb on - strandat 35.382 kb on - strandat 35.382 kb on - strandat 35.382 kb on - strandat 35.423 kb on - strandat 35.428 kb on + strandat 35.429 kb on - strandat 35.899 kb on + strand, within Psest_0035at 35.899 kb on + strand, within Psest_0035at 35.899 kb on + strand, within Psest_0035at 35.899 kb on + strand, within Psest_0035at 35.899 kb on + strand, within Psest_0035at 35.900 kb on - strand, within Psest_0035at 35.900 kb on - strand, within Psest_0035at 35.900 kb on - strand, within Psest_0035at 36.040 kb on + strand, within Psest_0035at 36.041 kb on - strand, within Psest_0035at 36.041 kb on - strand, within Psest_0035at 36.303 kb on - strand, within Psest_0035at 36.303 kb on - strand, within Psest_0035

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml
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32,454 + Psest_0032 0.77 +0.5
32,454 + Psest_0032 0.77 +1.9
32,455 - Psest_0032 0.77 +1.2
32,455 - Psest_0032 0.77 +1.5
32,506 + Psest_0032 0.82 -0.1
32,506 + Psest_0032 0.82 +1.2
32,506 + Psest_0032 0.82 +0.8
32,506 + Psest_0032 0.82 +1.2
32,506 + Psest_0032 0.82 +0.3
32,506 + Psest_0032 0.82 +1.5
32,506 + Psest_0032 0.82 +0.4
32,507 - Psest_0032 0.83 -0.2
32,507 - Psest_0032 0.83 +0.8
32,507 - Psest_0032 0.83 +1.4
32,507 - Psest_0032 0.83 +1.2
32,507 - Psest_0032 0.83 +1.1
32,507 - Psest_0032 0.83 +0.3
32,507 - Psest_0032 0.83 +1.7
32,514 + Psest_0032 0.83 +0.5
32,590 + +2.5
32,591 - +0.1
32,591 - +0.0
32,591 - +0.6
32,736 + +0.3
32,778 + -0.4
33,259 + +0.5
33,259 + -1.0
33,434 + +1.1
33,434 + -0.8
33,435 - +0.7
33,435 - +0.7
33,593 + Psest_0034 0.11 -1.5
33,593 + Psest_0034 0.11 -0.8
33,594 - Psest_0034 0.11 +0.9
33,764 + Psest_0034 0.20 -0.3
33,842 + Psest_0034 0.24 -1.0
33,842 + Psest_0034 0.24 -0.2
33,843 - Psest_0034 0.24 +1.4
33,843 - Psest_0034 0.24 +1.2
33,843 - Psest_0034 0.24 +0.8
33,843 - Psest_0034 0.24 +1.1
33,890 + Psest_0034 0.26 -0.7
33,920 + Psest_0034 0.27 -0.3
33,920 + Psest_0034 0.27 +0.8
33,921 - Psest_0034 0.27 +0.6
33,921 - Psest_0034 0.27 +0.8
33,921 - Psest_0034 0.27 -0.1
33,980 + Psest_0034 0.30 -0.3
33,980 + Psest_0034 0.30 -0.6
33,980 + Psest_0034 0.30 +0.4
33,980 + Psest_0034 0.30 +0.4
33,980 + Psest_0034 0.30 +0.9
33,981 - Psest_0034 0.30 +1.0
33,981 - Psest_0034 0.30 +0.6
33,981 - Psest_0034 0.30 +1.1
34,061 + Psest_0034 0.34 +1.7
34,061 + Psest_0034 0.34 -0.4
34,061 + Psest_0034 0.34 -0.3
34,062 - Psest_0034 0.34 +1.7
34,079 - Psest_0034 0.35 -1.3
34,109 + Psest_0034 0.37 +0.9
34,110 - Psest_0034 0.37 +0.5
34,136 + Psest_0034 0.38 -0.1
34,179 - Psest_0034 0.40 +0.8
34,346 - Psest_0034 0.48 +0.8
34,364 + Psest_0034 0.49 +0.4
34,413 - Psest_0034 0.51 +1.4
34,413 - Psest_0034 0.51 -0.1
34,527 - Psest_0034 0.57 +0.6
34,527 - Psest_0034 0.57 +0.4
34,535 + Psest_0034 0.57 -0.1
34,536 - Psest_0034 0.57 +0.8
34,544 + Psest_0034 0.58 -0.7
34,545 - Psest_0034 0.58 +0.4
34,649 + Psest_0034 0.63 -0.6
34,700 + Psest_0034 0.65 +1.5
34,700 + Psest_0034 0.65 +0.6
34,700 + Psest_0034 0.65 +0.7
34,700 + Psest_0034 0.65 -0.5
34,701 - Psest_0034 0.65 +0.2
34,701 - Psest_0034 0.65 +0.1
34,701 - Psest_0034 0.65 +0.4
34,701 - Psest_0034 0.65 +0.4
34,701 - Psest_0034 0.65 -0.3
34,709 - Psest_0034 0.66 +0.1
34,727 + Psest_0034 0.67 -0.4
34,775 + Psest_0034 0.69 +0.1
34,776 - Psest_0034 0.69 +1.2
35,118 - Psest_0034 0.86 +1.7
35,118 - Psest_0034 0.86 +1.5
35,135 + Psest_0034 0.87 +1.7
35,136 - Psest_0034 0.87 +2.8
35,136 - Psest_0034 0.87 +0.7
35,189 + Psest_0034 0.89 +2.7
35,190 - Psest_0034 0.89 +0.6
35,192 + Psest_0034 0.89 -0.2
35,193 - Psest_0034 0.90 +1.7
35,193 - Psest_0034 0.90 +1.1
35,222 + +1.7
35,223 - +1.3
35,223 - +0.8
35,232 - +1.2
35,259 - +1.3
35,318 + +1.9
35,318 + -0.0
35,318 + -0.3
35,318 + +1.8
35,319 - +0.9
35,324 + -0.1
35,324 + -0.3
35,324 + +1.0
35,325 - +0.1
35,325 - +1.1
35,325 - +2.0
35,325 - +0.5
35,325 - +1.7
35,325 - +0.8
35,325 - +1.4
35,381 + +1.3
35,381 + -0.1
35,381 + +1.1
35,381 + +1.5
35,382 - +0.4
35,382 - +0.6
35,382 - +1.2
35,382 - +2.5
35,382 - +2.3
35,423 - +1.0
35,428 + -1.0
35,429 - +0.7
35,899 + Psest_0035 0.32 +0.4
35,899 + Psest_0035 0.32 +0.5
35,899 + Psest_0035 0.32 +0.4
35,899 + Psest_0035 0.32 +1.4
35,899 + Psest_0035 0.32 +1.2
35,900 - Psest_0035 0.32 -1.6
35,900 - Psest_0035 0.32 +0.3
35,900 - Psest_0035 0.32 +0.9
36,040 + Psest_0035 0.41 +0.9
36,041 - Psest_0035 0.41 +2.3
36,041 - Psest_0035 0.41 +0.9
36,303 - Psest_0035 0.58 +0.1
36,303 - Psest_0035 0.58 -0.1

Or see this region's nucleotide sequence