Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3008 and Psest_3009 overlap by 4 nucleotides Psest_3009 and Psest_3010 overlap by 8 nucleotides Psest_3010 and Psest_3011 overlap by 4 nucleotides
Psest_3008: Psest_3008 - cobyric acid synthase CobQ, at 3,196,118 to 3,197,575
_3008
Psest_3009: Psest_3009 - L-threonine-O-3-phosphate decarboxylase, at 3,197,572 to 3,198,561
_3009
Psest_3010: Psest_3010 - cobalamin biosynthesis protein CobD, at 3,198,554 to 3,199,465
_3010
Psest_3011: Psest_3011 - ABC-type Fe3+-hydroxamate transport system, periplasmic component, at 3,199,462 to 3,200,262
_3011
Position (kb)
3197
3198
3199 Strain fitness (log2 ratio)
-1
0
1
2 at 3196.754 kb on + strand, within Psest_3008 at 3196.754 kb on + strand, within Psest_3008 at 3196.755 kb on - strand, within Psest_3008 at 3197.260 kb on - strand, within Psest_3008 at 3197.260 kb on - strand, within Psest_3008 at 3198.276 kb on - strand, within Psest_3009 at 3198.467 kb on + strand at 3198.518 kb on + strand at 3198.561 kb on + strand at 3198.561 kb on + strand at 3198.562 kb on - strand at 3198.562 kb on - strand at 3198.562 kb on - strand at 3198.700 kb on - strand, within Psest_3010 at 3198.700 kb on - strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.837 kb on + strand, within Psest_3010 at 3198.838 kb on - strand, within Psest_3010 at 3198.867 kb on + strand, within Psest_3010 at 3198.867 kb on + strand, within Psest_3010 at 3198.868 kb on - strand, within Psest_3010 at 3198.953 kb on - strand, within Psest_3010 at 3198.997 kb on + strand, within Psest_3010 at 3199.115 kb on + strand, within Psest_3010 at 3199.116 kb on - strand, within Psest_3010 at 3199.116 kb on - strand, within Psest_3010 at 3199.209 kb on + strand, within Psest_3010 at 3199.257 kb on - strand, within Psest_3010 at 3199.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 3,196,754 + Psest_3008 0.44 +1.6 3,196,754 + Psest_3008 0.44 -0.6 3,196,755 - Psest_3008 0.44 +0.8 3,197,260 - Psest_3008 0.78 -0.3 3,197,260 - Psest_3008 0.78 -0.6 3,198,276 - Psest_3009 0.71 +0.9 3,198,467 + -0.8 3,198,518 + +1.1 3,198,561 + -0.2 3,198,561 + -0.2 3,198,562 - +0.7 3,198,562 - +0.7 3,198,562 - +0.9 3,198,700 - Psest_3010 0.16 +1.7 3,198,700 - Psest_3010 0.16 +0.4 3,198,837 + Psest_3010 0.31 -0.3 3,198,837 + Psest_3010 0.31 -0.9 3,198,837 + Psest_3010 0.31 -1.3 3,198,837 + Psest_3010 0.31 -0.4 3,198,837 + Psest_3010 0.31 +0.6 3,198,837 + Psest_3010 0.31 +1.0 3,198,837 + Psest_3010 0.31 +1.3 3,198,837 + Psest_3010 0.31 +1.3 3,198,837 + Psest_3010 0.31 -1.4 3,198,837 + Psest_3010 0.31 +0.3 3,198,837 + Psest_3010 0.31 -0.3 3,198,837 + Psest_3010 0.31 +2.0 3,198,837 + Psest_3010 0.31 -0.5 3,198,838 - Psest_3010 0.31 -0.1 3,198,867 + Psest_3010 0.34 +0.6 3,198,867 + Psest_3010 0.34 -1.0 3,198,868 - Psest_3010 0.34 +0.7 3,198,953 - Psest_3010 0.44 -0.1 3,198,997 + Psest_3010 0.49 +0.5 3,199,115 + Psest_3010 0.62 +0.3 3,199,116 - Psest_3010 0.62 +1.1 3,199,116 - Psest_3010 0.62 +1.4 3,199,209 + Psest_3010 0.72 -1.3 3,199,257 - Psest_3010 0.77 +1.6 3,199,510 + +0.9
Or see this region's nucleotide sequence