Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3004 and Psest_3005 are separated by 10 nucleotides Psest_3005 and Psest_3006 overlap by 4 nucleotides Psest_3006 and Psest_3007 overlap by 1 nucleotides Psest_3007 and Psest_3008 are separated by 2 nucleotides
Psest_3004: Psest_3004 - cobalamin 5'-phosphate synthase/cobalamin synthase, at 3,193,231 to 3,193,962
_3004
Psest_3005: Psest_3005 - Fructose-2,6-bisphosphatase, at 3,193,973 to 3,194,551
_3005
Psest_3006: Psest_3006 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 3,194,548 to 3,195,597
_3006
Psest_3007: Psest_3007 - Adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase, at 3,195,597 to 3,196,115
_3007
Psest_3008: Psest_3008 - cobyric acid synthase CobQ, at 3,196,118 to 3,197,575
_3008
Position (kb)
3194
3195
3196 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3193.562 kb on + strand, within Psest_3004 at 3193.562 kb on + strand, within Psest_3004 at 3193.562 kb on + strand, within Psest_3004 at 3193.562 kb on + strand, within Psest_3004 at 3193.563 kb on - strand, within Psest_3004 at 3193.563 kb on - strand, within Psest_3004 at 3193.563 kb on - strand, within Psest_3004 at 3193.606 kb on + strand, within Psest_3004 at 3193.738 kb on + strand, within Psest_3004 at 3193.863 kb on - strand, within Psest_3004 at 3193.865 kb on + strand, within Psest_3004 at 3193.865 kb on + strand, within Psest_3004 at 3193.865 kb on + strand, within Psest_3004 at 3193.865 kb on + strand, within Psest_3004 at 3193.866 kb on - strand, within Psest_3004 at 3193.866 kb on - strand, within Psest_3004 at 3193.866 kb on - strand, within Psest_3004 at 3193.866 kb on - strand, within Psest_3004 at 3193.866 kb on - strand, within Psest_3004 at 3193.940 kb on - strand at 3193.951 kb on + strand at 3194.042 kb on + strand, within Psest_3005 at 3194.218 kb on + strand, within Psest_3005 at 3194.219 kb on - strand, within Psest_3005 at 3194.855 kb on + strand, within Psest_3006 at 3194.856 kb on - strand, within Psest_3006 at 3194.856 kb on - strand, within Psest_3006 at 3194.856 kb on - strand, within Psest_3006 at 3194.984 kb on - strand, within Psest_3006 at 3194.984 kb on - strand, within Psest_3006 at 3194.984 kb on - strand, within Psest_3006 at 3195.097 kb on + strand, within Psest_3006 at 3195.098 kb on - strand, within Psest_3006 at 3195.098 kb on - strand, within Psest_3006 at 3195.098 kb on - strand, within Psest_3006 at 3195.420 kb on + strand, within Psest_3006 at 3195.420 kb on + strand, within Psest_3006 at 3195.420 kb on + strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.421 kb on - strand, within Psest_3006 at 3195.425 kb on - strand, within Psest_3006 at 3195.425 kb on - strand, within Psest_3006 at 3196.027 kb on + strand, within Psest_3007 at 3196.027 kb on + strand, within Psest_3007 at 3196.027 kb on + strand, within Psest_3007 at 3196.027 kb on + strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.028 kb on - strand, within Psest_3007 at 3196.218 kb on + strand at 3196.218 kb on + strand at 3196.302 kb on + strand, within Psest_3008 at 3196.302 kb on + strand, within Psest_3008 at 3196.303 kb on - strand, within Psest_3008 at 3196.303 kb on - strand, within Psest_3008
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 3,193,562 + Psest_3004 0.45 -0.3 3,193,562 + Psest_3004 0.45 +0.2 3,193,562 + Psest_3004 0.45 +1.0 3,193,562 + Psest_3004 0.45 +0.5 3,193,563 - Psest_3004 0.45 +0.2 3,193,563 - Psest_3004 0.45 +0.9 3,193,563 - Psest_3004 0.45 -0.2 3,193,606 + Psest_3004 0.51 +1.0 3,193,738 + Psest_3004 0.69 +0.6 3,193,863 - Psest_3004 0.86 +0.1 3,193,865 + Psest_3004 0.87 +0.8 3,193,865 + Psest_3004 0.87 -0.9 3,193,865 + Psest_3004 0.87 +0.9 3,193,865 + Psest_3004 0.87 +1.3 3,193,866 - Psest_3004 0.87 +2.5 3,193,866 - Psest_3004 0.87 +1.8 3,193,866 - Psest_3004 0.87 +0.0 3,193,866 - Psest_3004 0.87 +2.3 3,193,866 - Psest_3004 0.87 +0.7 3,193,940 - +1.7 3,193,951 + -0.0 3,194,042 + Psest_3005 0.12 +0.3 3,194,218 + Psest_3005 0.42 +0.2 3,194,219 - Psest_3005 0.42 +0.7 3,194,855 + Psest_3006 0.29 +1.0 3,194,856 - Psest_3006 0.29 +3.0 3,194,856 - Psest_3006 0.29 +0.4 3,194,856 - Psest_3006 0.29 +1.1 3,194,984 - Psest_3006 0.42 +1.0 3,194,984 - Psest_3006 0.42 +2.5 3,194,984 - Psest_3006 0.42 +2.0 3,195,097 + Psest_3006 0.52 +0.2 3,195,098 - Psest_3006 0.52 +1.6 3,195,098 - Psest_3006 0.52 -0.0 3,195,098 - Psest_3006 0.52 +0.8 3,195,420 + Psest_3006 0.83 +1.2 3,195,420 + Psest_3006 0.83 +0.1 3,195,420 + Psest_3006 0.83 -0.2 3,195,421 - Psest_3006 0.83 +1.6 3,195,421 - Psest_3006 0.83 +1.3 3,195,421 - Psest_3006 0.83 +0.7 3,195,421 - Psest_3006 0.83 +2.0 3,195,425 - Psest_3006 0.84 -0.5 3,195,425 - Psest_3006 0.84 +1.8 3,196,027 + Psest_3007 0.83 -0.5 3,196,027 + Psest_3007 0.83 -1.2 3,196,027 + Psest_3007 0.83 +0.2 3,196,027 + Psest_3007 0.83 -0.0 3,196,028 - Psest_3007 0.83 +0.9 3,196,028 - Psest_3007 0.83 +0.9 3,196,028 - Psest_3007 0.83 -0.8 3,196,028 - Psest_3007 0.83 +1.7 3,196,028 - Psest_3007 0.83 +1.0 3,196,028 - Psest_3007 0.83 -0.0 3,196,028 - Psest_3007 0.83 -0.3 3,196,028 - Psest_3007 0.83 +0.4 3,196,028 - Psest_3007 0.83 +0.8 3,196,028 - Psest_3007 0.83 -0.0 3,196,028 - Psest_3007 0.83 -0.8 3,196,028 - Psest_3007 0.83 +0.3 3,196,028 - Psest_3007 0.83 +1.1 3,196,028 - Psest_3007 0.83 +0.5 3,196,218 + +0.6 3,196,218 + +0.2 3,196,302 + Psest_3008 0.13 -0.5 3,196,302 + Psest_3008 0.13 -1.0 3,196,303 - Psest_3008 0.13 +2.0 3,196,303 - Psest_3008 0.13 -0.1
Or see this region's nucleotide sequence