Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3006

Experiment: Def Polymyxin B sulfate 0.00008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3004 and Psest_3005 are separated by 10 nucleotidesPsest_3005 and Psest_3006 overlap by 4 nucleotidesPsest_3006 and Psest_3007 overlap by 1 nucleotidesPsest_3007 and Psest_3008 are separated by 2 nucleotides Psest_3004: Psest_3004 - cobalamin 5'-phosphate synthase/cobalamin synthase, at 3,193,231 to 3,193,962 _3004 Psest_3005: Psest_3005 - Fructose-2,6-bisphosphatase, at 3,193,973 to 3,194,551 _3005 Psest_3006: Psest_3006 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 3,194,548 to 3,195,597 _3006 Psest_3007: Psest_3007 - Adenosyl cobinamide kinase/adenosyl cobinamide phosphate guanylyltransferase, at 3,195,597 to 3,196,115 _3007 Psest_3008: Psest_3008 - cobyric acid synthase CobQ, at 3,196,118 to 3,197,575 _3008 Position (kb) 3194 3195 3196Strain fitness (log2 ratio) -1 0 1 2 3at 3193.562 kb on + strand, within Psest_3004at 3193.562 kb on + strand, within Psest_3004at 3193.562 kb on + strand, within Psest_3004at 3193.562 kb on + strand, within Psest_3004at 3193.563 kb on - strand, within Psest_3004at 3193.563 kb on - strand, within Psest_3004at 3193.563 kb on - strand, within Psest_3004at 3193.606 kb on + strand, within Psest_3004at 3193.738 kb on + strand, within Psest_3004at 3193.863 kb on - strand, within Psest_3004at 3193.865 kb on + strand, within Psest_3004at 3193.865 kb on + strand, within Psest_3004at 3193.865 kb on + strand, within Psest_3004at 3193.865 kb on + strand, within Psest_3004at 3193.866 kb on - strand, within Psest_3004at 3193.866 kb on - strand, within Psest_3004at 3193.866 kb on - strand, within Psest_3004at 3193.866 kb on - strand, within Psest_3004at 3193.866 kb on - strand, within Psest_3004at 3193.940 kb on - strandat 3193.951 kb on + strandat 3194.042 kb on + strand, within Psest_3005at 3194.218 kb on + strand, within Psest_3005at 3194.219 kb on - strand, within Psest_3005at 3194.855 kb on + strand, within Psest_3006at 3194.856 kb on - strand, within Psest_3006at 3194.856 kb on - strand, within Psest_3006at 3194.856 kb on - strand, within Psest_3006at 3194.984 kb on - strand, within Psest_3006at 3194.984 kb on - strand, within Psest_3006at 3194.984 kb on - strand, within Psest_3006at 3195.097 kb on + strand, within Psest_3006at 3195.098 kb on - strand, within Psest_3006at 3195.098 kb on - strand, within Psest_3006at 3195.098 kb on - strand, within Psest_3006at 3195.420 kb on + strand, within Psest_3006at 3195.420 kb on + strand, within Psest_3006at 3195.420 kb on + strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.421 kb on - strand, within Psest_3006at 3195.425 kb on - strand, within Psest_3006at 3195.425 kb on - strand, within Psest_3006at 3196.027 kb on + strand, within Psest_3007at 3196.027 kb on + strand, within Psest_3007at 3196.027 kb on + strand, within Psest_3007at 3196.027 kb on + strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.028 kb on - strand, within Psest_3007at 3196.218 kb on + strandat 3196.218 kb on + strandat 3196.302 kb on + strand, within Psest_3008at 3196.302 kb on + strand, within Psest_3008at 3196.303 kb on - strand, within Psest_3008at 3196.303 kb on - strand, within Psest_3008

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml
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3,193,562 + Psest_3004 0.45 -0.3
3,193,562 + Psest_3004 0.45 +0.2
3,193,562 + Psest_3004 0.45 +1.0
3,193,562 + Psest_3004 0.45 +0.5
3,193,563 - Psest_3004 0.45 +0.2
3,193,563 - Psest_3004 0.45 +0.9
3,193,563 - Psest_3004 0.45 -0.2
3,193,606 + Psest_3004 0.51 +1.0
3,193,738 + Psest_3004 0.69 +0.6
3,193,863 - Psest_3004 0.86 +0.1
3,193,865 + Psest_3004 0.87 +0.8
3,193,865 + Psest_3004 0.87 -0.9
3,193,865 + Psest_3004 0.87 +0.9
3,193,865 + Psest_3004 0.87 +1.3
3,193,866 - Psest_3004 0.87 +2.5
3,193,866 - Psest_3004 0.87 +1.8
3,193,866 - Psest_3004 0.87 +0.0
3,193,866 - Psest_3004 0.87 +2.3
3,193,866 - Psest_3004 0.87 +0.7
3,193,940 - +1.7
3,193,951 + -0.0
3,194,042 + Psest_3005 0.12 +0.3
3,194,218 + Psest_3005 0.42 +0.2
3,194,219 - Psest_3005 0.42 +0.7
3,194,855 + Psest_3006 0.29 +1.0
3,194,856 - Psest_3006 0.29 +3.0
3,194,856 - Psest_3006 0.29 +0.4
3,194,856 - Psest_3006 0.29 +1.1
3,194,984 - Psest_3006 0.42 +1.0
3,194,984 - Psest_3006 0.42 +2.5
3,194,984 - Psest_3006 0.42 +2.0
3,195,097 + Psest_3006 0.52 +0.2
3,195,098 - Psest_3006 0.52 +1.6
3,195,098 - Psest_3006 0.52 -0.0
3,195,098 - Psest_3006 0.52 +0.8
3,195,420 + Psest_3006 0.83 +1.2
3,195,420 + Psest_3006 0.83 +0.1
3,195,420 + Psest_3006 0.83 -0.2
3,195,421 - Psest_3006 0.83 +1.6
3,195,421 - Psest_3006 0.83 +1.3
3,195,421 - Psest_3006 0.83 +0.7
3,195,421 - Psest_3006 0.83 +2.0
3,195,425 - Psest_3006 0.84 -0.5
3,195,425 - Psest_3006 0.84 +1.8
3,196,027 + Psest_3007 0.83 -0.5
3,196,027 + Psest_3007 0.83 -1.2
3,196,027 + Psest_3007 0.83 +0.2
3,196,027 + Psest_3007 0.83 -0.0
3,196,028 - Psest_3007 0.83 +0.9
3,196,028 - Psest_3007 0.83 +0.9
3,196,028 - Psest_3007 0.83 -0.8
3,196,028 - Psest_3007 0.83 +1.7
3,196,028 - Psest_3007 0.83 +1.0
3,196,028 - Psest_3007 0.83 -0.0
3,196,028 - Psest_3007 0.83 -0.3
3,196,028 - Psest_3007 0.83 +0.4
3,196,028 - Psest_3007 0.83 +0.8
3,196,028 - Psest_3007 0.83 -0.0
3,196,028 - Psest_3007 0.83 -0.8
3,196,028 - Psest_3007 0.83 +0.3
3,196,028 - Psest_3007 0.83 +1.1
3,196,028 - Psest_3007 0.83 +0.5
3,196,218 + +0.6
3,196,218 + +0.2
3,196,302 + Psest_3008 0.13 -0.5
3,196,302 + Psest_3008 0.13 -1.0
3,196,303 - Psest_3008 0.13 +2.0
3,196,303 - Psest_3008 0.13 -0.1

Or see this region's nucleotide sequence