Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2583

Experiment: Def Polymyxin B sulfate 0.00008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2582 and Psest_2583 overlap by 4 nucleotidesPsest_2583 and Psest_2584 are separated by 210 nucleotidesPsest_2584 and Psest_2585 are separated by 91 nucleotides Psest_2582: Psest_2582 - folylpolyglutamate synthase/dihydrofolate synthase, at 2,762,038 to 2,763,342 _2582 Psest_2583: Psest_2583 - acetyl-CoA carboxylase, carboxyl transferase, beta subunit, at 2,763,339 to 2,764,232 _2583 Psest_2584: Psest_2584 - Phosphoribosylanthranilate isomerase, at 2,764,443 to 2,765,066 _2584 Psest_2585: Psest_2585 - pseudouridylate synthase I, at 2,765,158 to 2,766,018 _2585 Position (kb) 2763 2764 2765Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2764.305 kb on - strandat 2764.305 kb on - strandat 2764.305 kb on - strandat 2764.390 kb on - strandat 2764.683 kb on - strand, within Psest_2584at 2764.695 kb on - strand, within Psest_2584at 2764.720 kb on - strand, within Psest_2584at 2764.896 kb on - strand, within Psest_2584at 2764.899 kb on - strand, within Psest_2584at 2764.977 kb on + strand, within Psest_2584at 2764.977 kb on + strand, within Psest_2584at 2764.978 kb on - strand, within Psest_2584at 2764.978 kb on - strand, within Psest_2584at 2764.978 kb on - strand, within Psest_2584at 2765.043 kb on + strandat 2765.044 kb on - strandat 2765.071 kb on + strandat 2765.071 kb on + strandat 2765.075 kb on + strandat 2765.075 kb on + strandat 2765.075 kb on + strandat 2765.075 kb on + strandat 2765.076 kb on - strandat 2765.080 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml
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2,764,305 - +1.4
2,764,305 - -0.5
2,764,305 - -0.3
2,764,390 - +0.5
2,764,683 - Psest_2584 0.38 -2.7
2,764,695 - Psest_2584 0.40 -4.3
2,764,720 - Psest_2584 0.44 -3.8
2,764,896 - Psest_2584 0.73 -3.1
2,764,899 - Psest_2584 0.73 -4.7
2,764,977 + Psest_2584 0.86 -2.2
2,764,977 + Psest_2584 0.86 -2.8
2,764,978 - Psest_2584 0.86 -3.0
2,764,978 - Psest_2584 0.86 -4.9
2,764,978 - Psest_2584 0.86 -2.9
2,765,043 + -2.0
2,765,044 - -1.6
2,765,071 + -3.6
2,765,071 + -1.7
2,765,075 + -2.2
2,765,075 + -5.4
2,765,075 + -2.2
2,765,075 + -3.6
2,765,076 - -1.3
2,765,080 - +0.2

Or see this region's nucleotide sequence