Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2063

Experiment: Def Polymyxin B sulfate 0.00008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2062 and Psest_2063 are separated by 63 nucleotidesPsest_2063 and Psest_2064 overlap by 20 nucleotides Psest_2062: Psest_2062 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,187,981 to 2,188,745 _2062 Psest_2063: Psest_2063 - Enoyl-[acyl-carrier-protein] reductase (NADH), at 2,188,809 to 2,189,603 _2063 Psest_2064: Psest_2064 - ABC-type uncharacterized transport system, duplicated ATPase component, at 2,189,584 to 2,191,245 _2064 Position (kb) 2188 2189 2190Strain fitness (log2 ratio) -2 -1 0 1at 2187.824 kb on - strandat 2188.172 kb on + strand, within Psest_2062at 2188.342 kb on + strand, within Psest_2062at 2188.342 kb on + strand, within Psest_2062at 2188.343 kb on - strand, within Psest_2062at 2188.343 kb on - strand, within Psest_2062at 2188.343 kb on - strand, within Psest_2062at 2188.410 kb on + strand, within Psest_2062at 2188.420 kb on - strand, within Psest_2062at 2188.420 kb on - strand, within Psest_2062at 2188.447 kb on - strand, within Psest_2062at 2188.790 kb on + strandat 2188.791 kb on - strandat 2188.794 kb on + strandat 2189.674 kb on - strandat 2189.674 kb on - strandat 2190.551 kb on - strand, within Psest_2064

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml
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2,187,824 - +0.0
2,188,172 + Psest_2062 0.25 +0.6
2,188,342 + Psest_2062 0.47 -1.0
2,188,342 + Psest_2062 0.47 -1.3
2,188,343 - Psest_2062 0.47 -0.3
2,188,343 - Psest_2062 0.47 +0.1
2,188,343 - Psest_2062 0.47 +0.5
2,188,410 + Psest_2062 0.56 +0.7
2,188,420 - Psest_2062 0.57 -1.9
2,188,420 - Psest_2062 0.57 -0.1
2,188,447 - Psest_2062 0.61 +0.5
2,188,790 + -0.2
2,188,791 - +1.1
2,188,794 + +0.0
2,189,674 - -0.2
2,189,674 - -0.8
2,190,551 - Psest_2064 0.58 -1.8

Or see this region's nucleotide sequence