Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1450 and Psest_1451 are separated by 157 nucleotides Psest_1451 and Psest_1452 are separated by 160 nucleotides Psest_1452 and Psest_1453 are separated by 48 nucleotides
Psest_1450: Psest_1450 - Transcriptional regulator, at 1,558,367 to 1,559,293
_1450
Psest_1451: Psest_1451 - Uncharacterized ABC-type transport system, permease components, at 1,559,451 to 1,560,041
_1451
Psest_1452: Psest_1452 - 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase, at 1,560,202 to 1,562,511
_1452
Psest_1453: Psest_1453 - Glycerol-3-phosphate dehydrogenase, at 1,562,560 to 1,564,104
_1453
Position (kb)
1560
1561
1562
1563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.401 kb on - strand at 1559.452 kb on + strand at 1559.474 kb on + strand at 1559.474 kb on + strand at 1559.474 kb on + strand at 1559.475 kb on - strand at 1559.475 kb on - strand at 1559.475 kb on - strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.477 kb on - strand at 1559.477 kb on - strand at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.744 kb on - strand, within Psest_1451 at 1559.744 kb on - strand, within Psest_1451 at 1560.105 kb on + strand at 1560.105 kb on + strand at 1560.106 kb on - strand at 1560.106 kb on - strand at 1560.124 kb on + strand at 1560.128 kb on + strand at 1560.129 kb on - strand at 1560.326 kb on + strand at 1560.326 kb on + strand at 1560.326 kb on + strand at 1560.326 kb on + strand at 1560.326 kb on + strand at 1560.326 kb on + strand at 1560.327 kb on - strand at 1560.513 kb on + strand, within Psest_1452 at 1560.513 kb on + strand, within Psest_1452 at 1560.568 kb on + strand, within Psest_1452 at 1560.568 kb on + strand, within Psest_1452 at 1560.569 kb on - strand, within Psest_1452 at 1560.575 kb on - strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.700 kb on + strand, within Psest_1452 at 1560.701 kb on - strand, within Psest_1452 at 1560.701 kb on - strand, within Psest_1452 at 1560.715 kb on - strand, within Psest_1452 at 1560.782 kb on - strand, within Psest_1452 at 1560.782 kb on - strand, within Psest_1452 at 1560.896 kb on - strand, within Psest_1452 at 1560.896 kb on - strand, within Psest_1452 at 1560.896 kb on - strand, within Psest_1452 at 1560.896 kb on - strand, within Psest_1452 at 1560.940 kb on + strand, within Psest_1452 at 1560.940 kb on + strand, within Psest_1452 at 1560.941 kb on - strand, within Psest_1452 at 1560.941 kb on - strand, within Psest_1452 at 1560.941 kb on - strand, within Psest_1452 at 1560.941 kb on - strand, within Psest_1452 at 1561.060 kb on + strand, within Psest_1452 at 1561.060 kb on + strand, within Psest_1452 at 1561.060 kb on + strand, within Psest_1452 at 1561.061 kb on - strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.570 kb on + strand, within Psest_1452 at 1561.571 kb on - strand, within Psest_1452 at 1561.571 kb on - strand, within Psest_1452 at 1561.571 kb on - strand, within Psest_1452 at 1561.571 kb on - strand, within Psest_1452 at 1561.571 kb on - strand, within Psest_1452 at 1561.571 kb on - strand, within Psest_1452 at 1561.572 kb on + strand, within Psest_1452 at 1561.572 kb on + strand, within Psest_1452 at 1561.573 kb on - strand, within Psest_1452 at 1561.600 kb on + strand, within Psest_1452 at 1561.600 kb on + strand, within Psest_1452 at 1561.600 kb on + strand, within Psest_1452 at 1561.667 kb on + strand, within Psest_1452 at 1561.668 kb on - strand, within Psest_1452 at 1561.668 kb on - strand, within Psest_1452 at 1561.708 kb on + strand, within Psest_1452 at 1561.708 kb on + strand, within Psest_1452 at 1561.708 kb on + strand, within Psest_1452 at 1561.708 kb on + strand, within Psest_1452 at 1561.766 kb on - strand, within Psest_1452 at 1561.766 kb on - strand, within Psest_1452 at 1561.766 kb on - strand, within Psest_1452 at 1561.798 kb on + strand, within Psest_1452 at 1562.081 kb on - strand, within Psest_1452 at 1562.161 kb on + strand, within Psest_1452 at 1562.161 kb on + strand, within Psest_1452 at 1562.162 kb on - strand, within Psest_1452 at 1562.162 kb on - strand, within Psest_1452 at 1562.162 kb on - strand, within Psest_1452 at 1562.162 kb on - strand, within Psest_1452 at 1562.303 kb on - strand at 1562.723 kb on + strand, within Psest_1453 at 1563.080 kb on + strand, within Psest_1453 at 1563.143 kb on + strand, within Psest_1453 at 1563.143 kb on + strand, within Psest_1453 at 1563.143 kb on + strand, within Psest_1453 at 1563.143 kb on + strand, within Psest_1453 at 1563.143 kb on + strand, within Psest_1453 at 1563.144 kb on - strand, within Psest_1453 at 1563.144 kb on - strand, within Psest_1453 at 1563.209 kb on + strand, within Psest_1453 at 1563.258 kb on - strand, within Psest_1453 at 1563.258 kb on - strand, within Psest_1453 at 1563.345 kb on - strand, within Psest_1453 at 1563.345 kb on - strand, within Psest_1453 at 1563.345 kb on - strand, within Psest_1453 at 1563.345 kb on - strand, within Psest_1453 at 1563.362 kb on + strand, within Psest_1453 at 1563.362 kb on + strand, within Psest_1453 at 1563.363 kb on - strand, within Psest_1453 at 1563.363 kb on - strand, within Psest_1453
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 1,559,302 - -2.1 1,559,302 - -4.4 1,559,302 - -0.9 1,559,302 - +0.3 1,559,302 - -2.9 1,559,302 - -2.8 1,559,302 - -1.5 1,559,401 - -0.8 1,559,452 + -1.0 1,559,474 + -0.1 1,559,474 + -0.3 1,559,474 + -0.3 1,559,475 - +0.7 1,559,475 - +0.3 1,559,475 - +0.7 1,559,476 + -1.2 1,559,476 + -1.2 1,559,476 + -0.3 1,559,476 + -1.0 1,559,476 + -1.7 1,559,476 + -0.8 1,559,476 + +0.6 1,559,476 + -0.8 1,559,476 + +0.9 1,559,477 - -0.6 1,559,477 - -1.2 1,559,743 + Psest_1451 0.49 -0.7 1,559,743 + Psest_1451 0.49 -0.2 1,559,743 + Psest_1451 0.49 -0.6 1,559,743 + Psest_1451 0.49 +0.5 1,559,744 - Psest_1451 0.50 -1.5 1,559,744 - Psest_1451 0.50 -1.8 1,560,105 + -3.4 1,560,105 + -2.3 1,560,106 - -2.3 1,560,106 - -1.8 1,560,124 + -3.0 1,560,128 + -2.5 1,560,129 - -1.1 1,560,326 + -2.2 1,560,326 + -2.5 1,560,326 + -3.3 1,560,326 + -3.3 1,560,326 + -1.1 1,560,326 + -3.6 1,560,327 - -0.1 1,560,513 + Psest_1452 0.13 -1.4 1,560,513 + Psest_1452 0.13 -2.3 1,560,568 + Psest_1452 0.16 -2.7 1,560,568 + Psest_1452 0.16 -1.3 1,560,569 - Psest_1452 0.16 -2.4 1,560,575 - Psest_1452 0.16 -3.6 1,560,700 + Psest_1452 0.22 -0.9 1,560,700 + Psest_1452 0.22 -2.0 1,560,700 + Psest_1452 0.22 -2.1 1,560,700 + Psest_1452 0.22 -2.2 1,560,700 + Psest_1452 0.22 -3.4 1,560,700 + Psest_1452 0.22 -3.7 1,560,700 + Psest_1452 0.22 -4.3 1,560,700 + Psest_1452 0.22 -0.4 1,560,701 - Psest_1452 0.22 -1.8 1,560,701 - Psest_1452 0.22 -4.6 1,560,715 - Psest_1452 0.22 -1.4 1,560,782 - Psest_1452 0.25 -2.9 1,560,782 - Psest_1452 0.25 -1.2 1,560,896 - Psest_1452 0.30 -2.7 1,560,896 - Psest_1452 0.30 -2.7 1,560,896 - Psest_1452 0.30 -2.4 1,560,896 - Psest_1452 0.30 -3.9 1,560,940 + Psest_1452 0.32 -0.9 1,560,940 + Psest_1452 0.32 -2.7 1,560,941 - Psest_1452 0.32 -2.0 1,560,941 - Psest_1452 0.32 -1.4 1,560,941 - Psest_1452 0.32 -2.6 1,560,941 - Psest_1452 0.32 +0.1 1,561,060 + Psest_1452 0.37 -0.8 1,561,060 + Psest_1452 0.37 -2.5 1,561,060 + Psest_1452 0.37 -1.7 1,561,061 - Psest_1452 0.37 -1.8 1,561,570 + Psest_1452 0.59 -3.0 1,561,570 + Psest_1452 0.59 -1.8 1,561,570 + Psest_1452 0.59 -1.7 1,561,570 + Psest_1452 0.59 -3.0 1,561,570 + Psest_1452 0.59 -1.7 1,561,570 + Psest_1452 0.59 -1.1 1,561,570 + Psest_1452 0.59 -2.2 1,561,571 - Psest_1452 0.59 -2.2 1,561,571 - Psest_1452 0.59 -1.9 1,561,571 - Psest_1452 0.59 -2.9 1,561,571 - Psest_1452 0.59 -3.4 1,561,571 - Psest_1452 0.59 -3.0 1,561,571 - Psest_1452 0.59 -1.8 1,561,572 + Psest_1452 0.59 -0.1 1,561,572 + Psest_1452 0.59 -2.2 1,561,573 - Psest_1452 0.59 -1.2 1,561,600 + Psest_1452 0.61 -1.4 1,561,600 + Psest_1452 0.61 -0.4 1,561,600 + Psest_1452 0.61 -1.6 1,561,667 + Psest_1452 0.63 -3.0 1,561,668 - Psest_1452 0.63 -2.7 1,561,668 - Psest_1452 0.63 -1.7 1,561,708 + Psest_1452 0.65 -2.2 1,561,708 + Psest_1452 0.65 -2.2 1,561,708 + Psest_1452 0.65 -2.4 1,561,708 + Psest_1452 0.65 -3.3 1,561,766 - Psest_1452 0.68 -2.5 1,561,766 - Psest_1452 0.68 -4.0 1,561,766 - Psest_1452 0.68 -1.7 1,561,798 + Psest_1452 0.69 -1.7 1,562,081 - Psest_1452 0.81 -0.1 1,562,161 + Psest_1452 0.85 -2.1 1,562,161 + Psest_1452 0.85 -3.3 1,562,162 - Psest_1452 0.85 -2.5 1,562,162 - Psest_1452 0.85 -3.1 1,562,162 - Psest_1452 0.85 -2.4 1,562,162 - Psest_1452 0.85 -2.7 1,562,303 - -2.7 1,562,723 + Psest_1453 0.11 -1.0 1,563,080 + Psest_1453 0.34 -0.1 1,563,143 + Psest_1453 0.38 -0.1 1,563,143 + Psest_1453 0.38 +0.3 1,563,143 + Psest_1453 0.38 +0.5 1,563,143 + Psest_1453 0.38 +0.2 1,563,143 + Psest_1453 0.38 -0.2 1,563,144 - Psest_1453 0.38 +0.1 1,563,144 - Psest_1453 0.38 -0.3 1,563,209 + Psest_1453 0.42 +0.9 1,563,258 - Psest_1453 0.45 -0.9 1,563,258 - Psest_1453 0.45 +0.5 1,563,345 - Psest_1453 0.51 +0.3 1,563,345 - Psest_1453 0.51 +0.3 1,563,345 - Psest_1453 0.51 -0.7 1,563,345 - Psest_1453 0.51 +0.5 1,563,362 + Psest_1453 0.52 +1.2 1,563,362 + Psest_1453 0.52 -0.3 1,563,363 - Psest_1453 0.52 +0.2 1,563,363 - Psest_1453 0.52 +0.5
Or see this region's nucleotide sequence