Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1449 and Psest_1450 are separated by 8 nucleotides Psest_1450 and Psest_1451 are separated by 157 nucleotides Psest_1451 and Psest_1452 are separated by 160 nucleotides
Psest_1449: Psest_1449 - Short-chain dehydrogenases of various substrate specificities, at 1,557,384 to 1,558,358
_1449
Psest_1450: Psest_1450 - Transcriptional regulator, at 1,558,367 to 1,559,293
_1450
Psest_1451: Psest_1451 - Uncharacterized ABC-type transport system, permease components, at 1,559,451 to 1,560,041
_1451
Psest_1452: Psest_1452 - 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase, at 1,560,202 to 1,562,511
_1452
Position (kb)
1558
1559
1560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1557.418 kb on + strand at 1557.745 kb on - strand, within Psest_1449 at 1557.774 kb on + strand, within Psest_1449 at 1557.775 kb on - strand, within Psest_1449 at 1557.932 kb on + strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.991 kb on - strand at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1558.082 kb on + strand, within Psest_1449 at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.401 kb on - strand at 1559.452 kb on + strand at 1559.474 kb on + strand at 1559.474 kb on + strand at 1559.474 kb on + strand at 1559.475 kb on - strand at 1559.475 kb on - strand at 1559.475 kb on - strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.476 kb on + strand at 1559.477 kb on - strand at 1559.477 kb on - strand at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.743 kb on + strand, within Psest_1451 at 1559.744 kb on - strand, within Psest_1451 at 1559.744 kb on - strand, within Psest_1451 at 1560.105 kb on + strand at 1560.105 kb on + strand at 1560.106 kb on - strand at 1560.106 kb on - strand at 1560.124 kb on + strand at 1560.128 kb on + strand at 1560.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 1,557,418 + +0.9 1,557,745 - Psest_1449 0.37 -0.2 1,557,774 + Psest_1449 0.40 -1.0 1,557,775 - Psest_1449 0.40 +1.1 1,557,932 + Psest_1449 0.56 +0.0 1,557,933 - Psest_1449 0.56 +0.2 1,557,933 - Psest_1449 0.56 -0.7 1,557,933 - Psest_1449 0.56 +0.1 1,557,933 - Psest_1449 0.56 -0.4 1,557,990 + Psest_1449 0.62 -1.0 1,557,990 + Psest_1449 0.62 +0.4 1,557,990 + Psest_1449 0.62 -1.1 1,557,990 + Psest_1449 0.62 +0.2 1,557,990 + Psest_1449 0.62 -0.1 1,557,990 + Psest_1449 0.62 -0.1 1,557,990 + Psest_1449 0.62 -1.2 1,557,990 + Psest_1449 0.62 +0.3 1,557,990 + Psest_1449 0.62 -0.1 1,557,991 - +0.3 1,557,991 - Psest_1449 0.62 -0.5 1,557,991 - Psest_1449 0.62 -0.7 1,557,991 - Psest_1449 0.62 +0.4 1,557,991 - Psest_1449 0.62 -0.3 1,557,991 - Psest_1449 0.62 +1.3 1,557,991 - Psest_1449 0.62 -0.4 1,557,991 - Psest_1449 0.62 +2.2 1,557,991 - Psest_1449 0.62 +1.0 1,558,082 + Psest_1449 0.72 +0.1 1,559,302 - -2.1 1,559,302 - -4.4 1,559,302 - -0.9 1,559,302 - +0.3 1,559,302 - -2.9 1,559,302 - -2.8 1,559,302 - -1.5 1,559,401 - -0.8 1,559,452 + -1.0 1,559,474 + -0.1 1,559,474 + -0.3 1,559,474 + -0.3 1,559,475 - +0.7 1,559,475 - +0.3 1,559,475 - +0.7 1,559,476 + -1.2 1,559,476 + -1.2 1,559,476 + -0.3 1,559,476 + -1.0 1,559,476 + -1.7 1,559,476 + -0.8 1,559,476 + +0.6 1,559,476 + -0.8 1,559,476 + +0.9 1,559,477 - -0.6 1,559,477 - -1.2 1,559,743 + Psest_1451 0.49 -0.7 1,559,743 + Psest_1451 0.49 -0.2 1,559,743 + Psest_1451 0.49 -0.6 1,559,743 + Psest_1451 0.49 +0.5 1,559,744 - Psest_1451 0.50 -1.5 1,559,744 - Psest_1451 0.50 -1.8 1,560,105 + -3.4 1,560,105 + -2.3 1,560,106 - -2.3 1,560,106 - -1.8 1,560,124 + -3.0 1,560,128 + -2.5 1,560,129 - -1.1
Or see this region's nucleotide sequence