Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0135 and Psest_0136 are separated by 109 nucleotides Psest_0136 and Psest_0137 overlap by 4 nucleotides Psest_0137 and Psest_0138 are separated by 64 nucleotides Psest_0138 and Psest_0139 are separated by 15 nucleotides
Psest_0135: Psest_0135 - Uncharacterized enzymes related to aldose 1-epimerase, at 134,749 to 135,651
_0135
Psest_0136: Psest_0136 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 135,761 to 136,450
_0136
Psest_0137: Psest_0137 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 136,447 to 137,142
_0137
Psest_0138: Psest_0138 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 137,207 to 137,962
_0138
Psest_0139: Psest_0139 - ABC-type histidine transport system, ATPase component, at 137,978 to 138,751
_0139
Position (kb) 136
137
138 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 135.543 kb on - strand, within Psest_0135 at 135.598 kb on + strand at 135.599 kb on - strand at 135.664 kb on - strand at 135.843 kb on + strand, within Psest_0136 at 135.843 kb on + strand, within Psest_0136 at 135.843 kb on + strand, within Psest_0136 at 135.843 kb on + strand, within Psest_0136 at 135.844 kb on - strand, within Psest_0136 at 135.844 kb on - strand, within Psest_0136 at 135.844 kb on - strand, within Psest_0136 at 135.844 kb on - strand, within Psest_0136 at 135.882 kb on + strand, within Psest_0136 at 135.882 kb on + strand, within Psest_0136 at 135.882 kb on + strand, within Psest_0136 at 135.882 kb on + strand, within Psest_0136 at 135.882 kb on + strand, within Psest_0136 at 135.883 kb on - strand, within Psest_0136 at 135.984 kb on + strand, within Psest_0136 at 135.985 kb on - strand, within Psest_0136 at 136.124 kb on - strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.125 kb on + strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.126 kb on - strand, within Psest_0136 at 136.182 kb on + strand, within Psest_0136 at 136.230 kb on + strand, within Psest_0136 at 136.230 kb on + strand, within Psest_0136 at 136.233 kb on + strand, within Psest_0136 at 136.233 kb on + strand, within Psest_0136 at 136.233 kb on + strand, within Psest_0136 at 136.233 kb on + strand, within Psest_0136 at 136.234 kb on - strand, within Psest_0136 at 136.234 kb on - strand, within Psest_0136 at 136.234 kb on - strand, within Psest_0136 at 136.234 kb on - strand, within Psest_0136 at 136.234 kb on - strand, within Psest_0136 at 136.284 kb on + strand, within Psest_0136 at 136.285 kb on - strand, within Psest_0136 at 136.285 kb on - strand, within Psest_0136 at 136.285 kb on - strand, within Psest_0136 at 136.417 kb on + strand at 136.417 kb on + strand at 136.418 kb on - strand at 136.418 kb on - strand at 136.418 kb on - strand at 136.418 kb on - strand at 136.418 kb on - strand at 136.418 kb on - strand at 136.500 kb on + strand at 136.532 kb on + strand, within Psest_0137 at 136.532 kb on + strand, within Psest_0137 at 136.532 kb on + strand, within Psest_0137 at 136.532 kb on + strand, within Psest_0137 at 136.532 kb on + strand, within Psest_0137 at 136.533 kb on - strand, within Psest_0137 at 136.533 kb on - strand, within Psest_0137 at 136.533 kb on - strand, within Psest_0137 at 136.533 kb on - strand, within Psest_0137 at 136.630 kb on + strand, within Psest_0137 at 136.630 kb on + strand, within Psest_0137 at 136.630 kb on + strand, within Psest_0137 at 136.631 kb on - strand, within Psest_0137 at 136.631 kb on - strand, within Psest_0137 at 136.631 kb on - strand, within Psest_0137 at 136.631 kb on - strand, within Psest_0137 at 136.928 kb on + strand, within Psest_0137 at 136.928 kb on + strand, within Psest_0137 at 136.928 kb on + strand, within Psest_0137 at 136.928 kb on + strand, within Psest_0137 at 136.929 kb on - strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.979 kb on + strand, within Psest_0137 at 136.980 kb on - strand, within Psest_0137 at 136.980 kb on - strand, within Psest_0137 at 136.983 kb on + strand, within Psest_0137 at 137.009 kb on + strand, within Psest_0137 at 137.010 kb on - strand, within Psest_0137 at 137.142 kb on + strand at 137.142 kb on + strand at 137.143 kb on - strand at 137.143 kb on - strand at 137.143 kb on - strand at 137.211 kb on + strand at 137.211 kb on + strand at 137.212 kb on - strand at 137.212 kb on - strand at 137.229 kb on + strand at 137.229 kb on + strand at 137.229 kb on + strand at 137.229 kb on + strand at 137.229 kb on + strand at 137.229 kb on + strand at 137.229 kb on + strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.230 kb on - strand at 137.250 kb on + strand at 137.250 kb on + strand at 137.250 kb on + strand at 137.250 kb on + strand at 137.250 kb on + strand at 137.250 kb on + strand at 137.250 kb on + strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.251 kb on - strand at 137.464 kb on - strand, within Psest_0138 at 137.485 kb on - strand, within Psest_0138 at 137.577 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.637 kb on + strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.638 kb on - strand, within Psest_0138 at 137.640 kb on + strand, within Psest_0138 at 137.640 kb on + strand, within Psest_0138 at 137.640 kb on + strand, within Psest_0138 at 137.641 kb on - strand, within Psest_0138 at 137.641 kb on - strand, within Psest_0138 at 137.641 kb on - strand, within Psest_0138 at 137.641 kb on - strand, within Psest_0138 at 137.641 kb on - strand, within Psest_0138 at 137.641 kb on - strand, within Psest_0138 at 137.803 kb on + strand, within Psest_0138 at 137.804 kb on - strand, within Psest_0138 at 137.882 kb on + strand, within Psest_0138 at 137.950 kb on + strand at 137.950 kb on + strand at 137.950 kb on + strand at 137.950 kb on + strand at 137.950 kb on + strand at 137.950 kb on + strand at 137.950 kb on + strand at 137.950 kb on + strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.951 kb on - strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.952 kb on + strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.953 kb on - strand at 137.979 kb on + strand at 137.980 kb on - strand at 138.004 kb on + strand at 138.005 kb on - strand at 138.032 kb on + strand at 138.032 kb on + strand at 138.032 kb on + strand at 138.033 kb on - strand at 138.033 kb on - strand at 138.033 kb on - strand at 138.095 kb on + strand, within Psest_0139 at 138.096 kb on - strand, within Psest_0139 at 138.136 kb on + strand, within Psest_0139 at 138.136 kb on + strand, within Psest_0139 at 138.136 kb on + strand, within Psest_0139 at 138.137 kb on - strand, within Psest_0139
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 135,543 - Psest_0135 0.88 +0.0 135,598 + +0.3 135,599 - -0.2 135,664 - +0.4 135,843 + Psest_0136 0.12 -1.2 135,843 + Psest_0136 0.12 -1.5 135,843 + Psest_0136 0.12 -1.3 135,843 + Psest_0136 0.12 -0.7 135,844 - Psest_0136 0.12 +0.7 135,844 - Psest_0136 0.12 -1.8 135,844 - Psest_0136 0.12 -1.6 135,844 - Psest_0136 0.12 -0.6 135,882 + Psest_0136 0.18 -2.7 135,882 + Psest_0136 0.18 -1.1 135,882 + Psest_0136 0.18 -1.7 135,882 + Psest_0136 0.18 -1.1 135,882 + Psest_0136 0.18 -0.7 135,883 - Psest_0136 0.18 -0.9 135,984 + Psest_0136 0.32 -1.1 135,985 - Psest_0136 0.32 -1.0 136,124 - Psest_0136 0.53 -2.2 136,125 + Psest_0136 0.53 -1.3 136,125 + Psest_0136 0.53 -2.2 136,125 + Psest_0136 0.53 +1.1 136,125 + Psest_0136 0.53 -1.3 136,125 + Psest_0136 0.53 -1.5 136,125 + Psest_0136 0.53 -2.0 136,125 + Psest_0136 0.53 -0.3 136,125 + Psest_0136 0.53 -1.4 136,125 + Psest_0136 0.53 -1.1 136,125 + Psest_0136 0.53 -3.8 136,125 + Psest_0136 0.53 -1.7 136,125 + Psest_0136 0.53 -1.2 136,125 + Psest_0136 0.53 -2.7 136,125 + Psest_0136 0.53 -1.7 136,125 + Psest_0136 0.53 -1.5 136,126 - Psest_0136 0.53 -1.8 136,126 - Psest_0136 0.53 -2.1 136,126 - Psest_0136 0.53 -1.2 136,126 - Psest_0136 0.53 -1.6 136,126 - Psest_0136 0.53 +0.2 136,126 - Psest_0136 0.53 -0.8 136,126 - Psest_0136 0.53 -1.3 136,126 - Psest_0136 0.53 -1.2 136,126 - Psest_0136 0.53 -1.0 136,182 + Psest_0136 0.61 -1.4 136,230 + Psest_0136 0.68 -1.4 136,230 + Psest_0136 0.68 -0.7 136,233 + Psest_0136 0.68 -1.0 136,233 + Psest_0136 0.68 -2.1 136,233 + Psest_0136 0.68 -2.0 136,233 + Psest_0136 0.68 -1.9 136,234 - Psest_0136 0.69 -1.5 136,234 - Psest_0136 0.69 -2.0 136,234 - Psest_0136 0.69 -0.1 136,234 - Psest_0136 0.69 -1.1 136,234 - Psest_0136 0.69 -1.1 136,284 + Psest_0136 0.76 -1.6 136,285 - Psest_0136 0.76 -1.9 136,285 - Psest_0136 0.76 -3.2 136,285 - Psest_0136 0.76 -1.7 136,417 + -0.3 136,417 + -2.8 136,418 - -1.0 136,418 - -2.1 136,418 - -1.5 136,418 - +0.6 136,418 - -1.1 136,418 - -2.7 136,500 + -1.9 136,532 + Psest_0137 0.12 -1.8 136,532 + Psest_0137 0.12 -0.3 136,532 + Psest_0137 0.12 -0.9 136,532 + Psest_0137 0.12 -0.6 136,532 + Psest_0137 0.12 -1.3 136,533 - Psest_0137 0.12 +1.0 136,533 - Psest_0137 0.12 +0.7 136,533 - Psest_0137 0.12 -0.1 136,533 - Psest_0137 0.12 -1.6 136,630 + Psest_0137 0.26 -0.1 136,630 + Psest_0137 0.26 -1.9 136,630 + Psest_0137 0.26 -1.9 136,631 - Psest_0137 0.26 -2.5 136,631 - Psest_0137 0.26 -0.3 136,631 - Psest_0137 0.26 -0.8 136,631 - Psest_0137 0.26 -0.7 136,928 + Psest_0137 0.69 -1.4 136,928 + Psest_0137 0.69 -0.3 136,928 + Psest_0137 0.69 -1.8 136,928 + Psest_0137 0.69 -0.5 136,929 - Psest_0137 0.69 -1.6 136,979 + Psest_0137 0.76 -0.8 136,979 + Psest_0137 0.76 -0.9 136,979 + Psest_0137 0.76 -0.4 136,979 + Psest_0137 0.76 -1.5 136,979 + Psest_0137 0.76 +0.1 136,979 + Psest_0137 0.76 -1.4 136,979 + Psest_0137 0.76 -1.0 136,979 + Psest_0137 0.76 -1.4 136,980 - Psest_0137 0.77 -2.0 136,980 - Psest_0137 0.77 -1.2 136,983 + Psest_0137 0.77 -1.0 137,009 + Psest_0137 0.81 -1.2 137,010 - Psest_0137 0.81 -0.7 137,142 + -0.4 137,142 + -0.3 137,143 - -0.5 137,143 - -1.1 137,143 - -0.8 137,211 + -1.7 137,211 + -1.1 137,212 - +0.5 137,212 - +1.3 137,229 + -1.2 137,229 + -1.7 137,229 + -1.2 137,229 + -0.8 137,229 + -1.0 137,229 + -1.8 137,229 + -1.4 137,230 - -1.3 137,230 - -0.4 137,230 - -0.2 137,230 - +0.0 137,230 - -0.3 137,230 - -0.6 137,230 - -0.6 137,230 - -0.5 137,230 - -0.3 137,230 - -0.4 137,230 - -0.6 137,250 + -2.9 137,250 + -0.4 137,250 + -2.5 137,250 + -1.7 137,250 + -1.9 137,250 + -2.1 137,250 + -1.1 137,251 - +0.1 137,251 - -1.4 137,251 - -0.9 137,251 - +0.0 137,251 - -0.5 137,251 - -1.0 137,251 - -0.6 137,251 - -1.0 137,251 - +0.3 137,251 - -0.5 137,251 - -0.6 137,251 - -0.6 137,251 - -0.4 137,251 - -1.5 137,251 - -1.1 137,251 - -2.8 137,464 - Psest_0138 0.34 -1.2 137,485 - Psest_0138 0.37 -2.7 137,577 + Psest_0138 0.49 -1.1 137,637 + Psest_0138 0.57 -1.8 137,637 + Psest_0138 0.57 -2.0 137,637 + Psest_0138 0.57 -1.8 137,637 + Psest_0138 0.57 -1.4 137,637 + Psest_0138 0.57 -1.9 137,637 + Psest_0138 0.57 -0.8 137,637 + Psest_0138 0.57 -1.7 137,637 + Psest_0138 0.57 -1.3 137,638 - Psest_0138 0.57 -1.0 137,638 - Psest_0138 0.57 -1.8 137,638 - Psest_0138 0.57 -2.0 137,638 - Psest_0138 0.57 -1.2 137,638 - Psest_0138 0.57 -0.6 137,638 - Psest_0138 0.57 -1.3 137,638 - Psest_0138 0.57 +0.7 137,638 - Psest_0138 0.57 -0.6 137,640 + Psest_0138 0.57 -1.6 137,640 + Psest_0138 0.57 -1.9 137,640 + Psest_0138 0.57 -1.7 137,641 - Psest_0138 0.57 -1.7 137,641 - Psest_0138 0.57 -2.1 137,641 - Psest_0138 0.57 -1.2 137,641 - Psest_0138 0.57 -1.9 137,641 - Psest_0138 0.57 -1.1 137,641 - Psest_0138 0.57 -1.2 137,803 + Psest_0138 0.79 -1.9 137,804 - Psest_0138 0.79 -1.7 137,882 + Psest_0138 0.89 -1.1 137,950 + -2.1 137,950 + -1.4 137,950 + +1.4 137,950 + -1.6 137,950 + -1.3 137,950 + -0.4 137,950 + -1.3 137,950 + -1.8 137,951 - -2.1 137,951 - -1.5 137,951 - -0.6 137,951 - -1.0 137,951 - -0.1 137,951 - -1.6 137,951 - -0.8 137,951 - -1.0 137,951 - -1.9 137,952 + -0.5 137,952 + -1.0 137,952 + -0.9 137,952 + -0.7 137,952 + -0.6 137,952 + -2.1 137,952 + -0.4 137,952 + -1.6 137,952 + -1.6 137,952 + -1.2 137,952 + -1.7 137,952 + -2.4 137,952 + -0.2 137,953 - -1.2 137,953 - -2.8 137,953 - -1.0 137,953 - -1.7 137,953 - -1.1 137,953 - -1.0 137,953 - -1.2 137,953 - -2.0 137,953 - -2.3 137,953 - -0.6 137,953 - -0.9 137,953 - -1.3 137,953 - -1.5 137,979 + -0.7 137,980 - +1.3 138,004 + -3.0 138,005 - -0.7 138,032 + -1.0 138,032 + -0.4 138,032 + +0.2 138,033 - -0.3 138,033 - -0.5 138,033 - -3.0 138,095 + Psest_0139 0.15 -1.7 138,096 - Psest_0139 0.15 +1.0 138,136 + Psest_0139 0.20 +0.4 138,136 + Psest_0139 0.20 -0.5 138,136 + Psest_0139 0.20 -0.8 138,137 - Psest_0139 0.21 -0.8
Or see this region's nucleotide sequence