Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1297

Experiment: Def Polymyxin B sulfate 0.00008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1296 and Psest_1297 are separated by 177 nucleotidesPsest_1297 and Psest_1298 are separated by 147 nucleotidesPsest_1298 and Psest_1299 are separated by 75 nucleotidesPsest_1299 and Psest_1300 are separated by 44 nucleotidesPsest_1300 and Psest_1301 are separated by 48 nucleotidesPsest_1301 and Psest_1302 are separated by 192 nucleotides Psest_1296: Psest_1296 - excinuclease ABC, B subunit, at 1,400,569 to 1,402,584 _1296 Psest_1297: Psest_1297 - glutamyl-tRNA synthetase, bacterial family, at 1,402,762 to 1,404,243 _1297 Psest_1298: Psest_1298 - tRNA_Ala_GGC, at 1,404,391 to 1,404,466 _1298 Psest_1299: Psest_1299 - tRNA_Glu_TTC, at 1,404,542 to 1,404,617 _1299 Psest_1300: Psest_1300 - tRNA_Ala_GGC, at 1,404,662 to 1,404,737 _1300 Psest_1301: Psest_1301 - tRNA_Glu_TTC, at 1,404,786 to 1,404,861 _1301 Psest_1302: Psest_1302 - Predicted thioesterase, at 1,405,054 to 1,405,503 _1302 Position (kb) 1402 1403 1404 1405Strain fitness (log2 ratio) -2 -1 0 1at 1401.955 kb on + strand, within Psest_1296at 1401.985 kb on + strand, within Psest_1296at 1402.219 kb on + strand, within Psest_1296at 1402.232 kb on + strand, within Psest_1296at 1404.234 kb on + strandat 1404.297 kb on - strandat 1404.307 kb on - strandat 1404.619 kb on + strandat 1404.655 kb on + strandat 1404.655 kb on + strandat 1405.017 kb on + strandat 1405.017 kb on + strandat 1405.018 kb on - strandat 1405.135 kb on - strand, within Psest_1302at 1405.144 kb on + strand, within Psest_1302at 1405.144 kb on + strand, within Psest_1302at 1405.145 kb on - strand, within Psest_1302at 1405.211 kb on - strand, within Psest_1302at 1405.211 kb on - strand, within Psest_1302at 1405.242 kb on - strand, within Psest_1302

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml
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1,401,955 + Psest_1296 0.69 +0.6
1,401,985 + Psest_1296 0.70 -0.2
1,402,219 + Psest_1296 0.82 -1.8
1,402,232 + Psest_1296 0.82 -0.5
1,404,234 + -0.1
1,404,297 - -1.1
1,404,307 - -1.0
1,404,619 + +1.3
1,404,655 + -1.0
1,404,655 + +0.9
1,405,017 + -0.9
1,405,017 + -1.9
1,405,018 - -1.1
1,405,135 - Psest_1302 0.18 -0.5
1,405,144 + Psest_1302 0.20 -1.5
1,405,144 + Psest_1302 0.20 -1.2
1,405,145 - Psest_1302 0.20 -0.2
1,405,211 - Psest_1302 0.35 -2.5
1,405,211 - Psest_1302 0.35 -0.1
1,405,242 - Psest_1302 0.42 +0.4

Or see this region's nucleotide sequence