Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1296 and Psest_1297 are separated by 177 nucleotides Psest_1297 and Psest_1298 are separated by 147 nucleotides Psest_1298 and Psest_1299 are separated by 75 nucleotides Psest_1299 and Psest_1300 are separated by 44 nucleotides Psest_1300 and Psest_1301 are separated by 48 nucleotides Psest_1301 and Psest_1302 are separated by 192 nucleotides
Psest_1296: Psest_1296 - excinuclease ABC, B subunit, at 1,400,569 to 1,402,584
_1296
Psest_1297: Psest_1297 - glutamyl-tRNA synthetase, bacterial family, at 1,402,762 to 1,404,243
_1297
Psest_1298: Psest_1298 - tRNA_Ala_GGC, at 1,404,391 to 1,404,466
_1298
Psest_1299: Psest_1299 - tRNA_Glu_TTC, at 1,404,542 to 1,404,617
_1299
Psest_1300: Psest_1300 - tRNA_Ala_GGC, at 1,404,662 to 1,404,737
_1300
Psest_1301: Psest_1301 - tRNA_Glu_TTC, at 1,404,786 to 1,404,861
_1301
Psest_1302: Psest_1302 - Predicted thioesterase, at 1,405,054 to 1,405,503
_1302
Position (kb)
1402
1403
1404
1405 Strain fitness (log2 ratio)
-2
-1
0
1 at 1401.955 kb on + strand, within Psest_1296 at 1401.985 kb on + strand, within Psest_1296 at 1402.219 kb on + strand, within Psest_1296 at 1402.232 kb on + strand, within Psest_1296 at 1404.234 kb on + strand at 1404.297 kb on - strand at 1404.307 kb on - strand at 1404.619 kb on + strand at 1404.655 kb on + strand at 1404.655 kb on + strand at 1405.017 kb on + strand at 1405.017 kb on + strand at 1405.018 kb on - strand at 1405.135 kb on - strand, within Psest_1302 at 1405.144 kb on + strand, within Psest_1302 at 1405.144 kb on + strand, within Psest_1302 at 1405.145 kb on - strand, within Psest_1302 at 1405.211 kb on - strand, within Psest_1302 at 1405.211 kb on - strand, within Psest_1302 at 1405.242 kb on - strand, within Psest_1302
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 1,401,955 + Psest_1296 0.69 +0.6 1,401,985 + Psest_1296 0.70 -0.2 1,402,219 + Psest_1296 0.82 -1.8 1,402,232 + Psest_1296 0.82 -0.5 1,404,234 + -0.1 1,404,297 - -1.1 1,404,307 - -1.0 1,404,619 + +1.3 1,404,655 + -1.0 1,404,655 + +0.9 1,405,017 + -0.9 1,405,017 + -1.9 1,405,018 - -1.1 1,405,135 - Psest_1302 0.18 -0.5 1,405,144 + Psest_1302 0.20 -1.5 1,405,144 + Psest_1302 0.20 -1.2 1,405,145 - Psest_1302 0.20 -0.2 1,405,211 - Psest_1302 0.35 -2.5 1,405,211 - Psest_1302 0.35 -0.1 1,405,242 - Psest_1302 0.42 +0.4
Or see this region's nucleotide sequence