Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1161 and Psest_1162 are separated by 14 nucleotides Psest_1162 and Psest_1163 are separated by 125 nucleotides Psest_1163 and Psest_1164 are separated by 282 nucleotides
Psest_1161: Psest_1161 - E3 Ubiquitin ligase., at 1,261,758 to 1,262,651
_1161
Psest_1162: Psest_1162 - Spermidine synthase, at 1,262,666 to 1,263,349
_1162
Psest_1163: Psest_1163 - diguanylate cyclase (GGDEF) domain, at 1,263,475 to 1,265,535
_1163
Psest_1164: Psest_1164 - Superoxide dismutase, at 1,265,818 to 1,266,399
_1164
Position (kb)
1263
1264
1265
1266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1262.636 kb on + strand at 1262.636 kb on + strand at 1263.078 kb on - strand, within Psest_1162 at 1263.078 kb on - strand, within Psest_1162 at 1263.156 kb on - strand, within Psest_1162 at 1263.336 kb on - strand at 1263.336 kb on - strand at 1263.336 kb on - strand at 1263.448 kb on - strand at 1264.212 kb on - strand, within Psest_1163 at 1264.506 kb on - strand, within Psest_1163 at 1264.641 kb on - strand, within Psest_1163 at 1264.641 kb on - strand, within Psest_1163 at 1264.693 kb on - strand, within Psest_1163 at 1264.725 kb on + strand, within Psest_1163 at 1264.804 kb on - strand, within Psest_1163 at 1264.830 kb on + strand, within Psest_1163 at 1264.831 kb on - strand, within Psest_1163 at 1264.831 kb on - strand, within Psest_1163 at 1265.000 kb on + strand, within Psest_1163 at 1265.001 kb on - strand, within Psest_1163 at 1265.130 kb on - strand, within Psest_1163 at 1265.130 kb on - strand, within Psest_1163 at 1265.138 kb on + strand, within Psest_1163 at 1265.346 kb on - strand at 1265.346 kb on - strand at 1265.346 kb on - strand at 1265.455 kb on + strand at 1265.632 kb on - strand at 1265.632 kb on - strand at 1265.632 kb on - strand at 1265.632 kb on - strand at 1265.675 kb on + strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.676 kb on - strand at 1265.678 kb on + strand at 1265.679 kb on - strand at 1265.679 kb on - strand at 1265.679 kb on - strand at 1265.679 kb on - strand at 1265.689 kb on - strand at 1265.689 kb on - strand at 1265.694 kb on - strand at 1265.694 kb on - strand at 1265.694 kb on - strand at 1265.732 kb on - strand at 1265.732 kb on - strand at 1265.739 kb on + strand at 1265.739 kb on + strand at 1265.739 kb on + strand at 1265.739 kb on + strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.740 kb on - strand at 1265.766 kb on - strand at 1265.766 kb on - strand at 1265.766 kb on - strand at 1265.766 kb on - strand at 1265.802 kb on - strand at 1265.819 kb on + strand at 1265.820 kb on - strand at 1266.412 kb on - strand at 1266.454 kb on - strand at 1266.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 1,262,636 + -0.9 1,262,636 + -0.4 1,263,078 - Psest_1162 0.60 -0.5 1,263,078 - Psest_1162 0.60 -0.4 1,263,156 - Psest_1162 0.72 +0.2 1,263,336 - +0.7 1,263,336 - +0.3 1,263,336 - +0.5 1,263,448 - -1.7 1,264,212 - Psest_1163 0.36 +0.3 1,264,506 - Psest_1163 0.50 -2.7 1,264,641 - Psest_1163 0.57 -2.2 1,264,641 - Psest_1163 0.57 -2.6 1,264,693 - Psest_1163 0.59 -1.6 1,264,725 + Psest_1163 0.61 -2.6 1,264,804 - Psest_1163 0.64 -2.3 1,264,830 + Psest_1163 0.66 -1.9 1,264,831 - Psest_1163 0.66 -1.9 1,264,831 - Psest_1163 0.66 -2.6 1,265,000 + Psest_1163 0.74 -2.6 1,265,001 - Psest_1163 0.74 -1.7 1,265,130 - Psest_1163 0.80 -2.8 1,265,130 - Psest_1163 0.80 -2.9 1,265,138 + Psest_1163 0.81 +0.3 1,265,346 - -1.4 1,265,346 - -2.8 1,265,346 - -0.7 1,265,455 + +0.1 1,265,632 - +1.9 1,265,632 - +1.9 1,265,632 - +2.2 1,265,632 - +2.6 1,265,675 + -1.1 1,265,676 - +1.8 1,265,676 - +1.9 1,265,676 - +1.9 1,265,676 - +2.6 1,265,676 - +1.8 1,265,676 - +1.2 1,265,676 - +1.9 1,265,676 - +2.1 1,265,676 - +1.4 1,265,676 - +0.8 1,265,676 - +2.2 1,265,678 + -0.4 1,265,679 - +2.2 1,265,679 - +1.8 1,265,679 - +1.6 1,265,679 - +2.3 1,265,689 - +2.1 1,265,689 - +2.0 1,265,694 - +1.8 1,265,694 - +2.0 1,265,694 - +2.0 1,265,732 - +1.7 1,265,732 - +1.1 1,265,739 + -0.9 1,265,739 + -0.9 1,265,739 + -1.3 1,265,739 + -0.7 1,265,740 - +1.6 1,265,740 - +1.7 1,265,740 - +2.4 1,265,740 - +1.6 1,265,740 - +2.0 1,265,740 - +2.6 1,265,740 - +3.2 1,265,740 - +2.3 1,265,740 - +1.6 1,265,740 - +1.3 1,265,740 - +2.1 1,265,740 - +2.0 1,265,740 - +1.7 1,265,740 - +1.8 1,265,740 - +2.4 1,265,740 - +0.4 1,265,740 - +2.5 1,265,740 - +2.4 1,265,766 - +1.8 1,265,766 - +1.9 1,265,766 - +1.9 1,265,766 - +2.1 1,265,802 - +1.1 1,265,819 + -1.0 1,265,820 - +0.7 1,266,412 - +0.2 1,266,454 - -1.0 1,266,533 - +0.8
Or see this region's nucleotide sequence