Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1112

Experiment: Def Polymyxin B sulfate 0.00008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1111 and Psest_1112 are separated by 197 nucleotidesPsest_1112 and Psest_1113 overlap by 4 nucleotides Psest_1111: Psest_1111 - Flp pilus assembly protein TadD, contains TPR repeats, at 1,204,625 to 1,206,352 _1111 Psest_1112: Psest_1112 - glutamyl-tRNA reductase, at 1,206,550 to 1,207,818 _1112 Psest_1113: Psest_1113 - peptide chain release factor 1, at 1,207,815 to 1,208,897 _1113 Position (kb) 1206 1207 1208Strain fitness (log2 ratio) -1 0 1at 1205.575 kb on - strand, within Psest_1111at 1205.667 kb on - strand, within Psest_1111at 1206.363 kb on + strandat 1206.510 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml
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1,205,575 - Psest_1111 0.55 -1.5
1,205,667 - Psest_1111 0.60 +0.1
1,206,363 + -1.6
1,206,510 + -1.5

Or see this region's nucleotide sequence