Experiment: Def Polymyxin B sulfate 0.00008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1069 and Psest_1070 are separated by 140 nucleotides Psest_1070 and Psest_1071 are separated by 111 nucleotides Psest_1071 and Psest_1072 are separated by 76 nucleotides
Psest_1069: Psest_1069 - Protease II, at 1,156,275 to 1,158,104
_1069
Psest_1070: Psest_1070 - Predicted membrane protein, at 1,158,245 to 1,158,643
_1070
Psest_1071: Psest_1071 - Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains, at 1,158,755 to 1,160,254
_1071
Psest_1072: Psest_1072 - TRAP transporter, DctM subunit, at 1,160,331 to 1,161,701
_1072
Position (kb)
1158
1159
1160
1161 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1157.976 kb on - strand at 1158.125 kb on + strand at 1158.125 kb on + strand at 1158.126 kb on - strand at 1158.238 kb on + strand at 1158.246 kb on + strand at 1158.246 kb on + strand at 1158.246 kb on + strand at 1158.247 kb on - strand at 1158.247 kb on - strand at 1158.247 kb on - strand at 1158.247 kb on - strand at 1158.247 kb on - strand at 1158.447 kb on + strand, within Psest_1070 at 1158.447 kb on + strand, within Psest_1070 at 1158.448 kb on - strand, within Psest_1070 at 1158.493 kb on - strand, within Psest_1070 at 1158.567 kb on - strand, within Psest_1070 at 1158.694 kb on + strand at 1158.695 kb on - strand at 1158.695 kb on - strand at 1158.695 kb on - strand at 1158.695 kb on - strand at 1158.695 kb on - strand at 1158.748 kb on - strand at 1158.792 kb on + strand at 1158.793 kb on - strand at 1158.793 kb on - strand at 1158.978 kb on + strand, within Psest_1071 at 1158.978 kb on + strand, within Psest_1071 at 1158.979 kb on - strand, within Psest_1071 at 1159.040 kb on + strand, within Psest_1071 at 1159.041 kb on - strand, within Psest_1071 at 1159.041 kb on - strand, within Psest_1071 at 1159.041 kb on - strand, within Psest_1071 at 1159.272 kb on + strand, within Psest_1071 at 1159.272 kb on + strand, within Psest_1071 at 1159.275 kb on + strand, within Psest_1071 at 1159.275 kb on + strand, within Psest_1071 at 1159.275 kb on + strand, within Psest_1071 at 1159.276 kb on - strand, within Psest_1071 at 1159.276 kb on - strand, within Psest_1071 at 1159.403 kb on + strand, within Psest_1071 at 1159.767 kb on + strand, within Psest_1071 at 1159.767 kb on + strand, within Psest_1071 at 1160.085 kb on + strand, within Psest_1071 at 1160.086 kb on - strand, within Psest_1071 at 1160.086 kb on - strand, within Psest_1071 at 1160.086 kb on - strand, within Psest_1071 at 1160.086 kb on - strand, within Psest_1071 at 1160.086 kb on - strand, within Psest_1071 at 1160.345 kb on - strand at 1160.383 kb on + strand at 1160.383 kb on + strand at 1160.383 kb on + strand at 1160.422 kb on + strand at 1160.440 kb on + strand at 1160.440 kb on + strand at 1160.441 kb on - strand at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.485 kb on + strand, within Psest_1072 at 1160.486 kb on - strand, within Psest_1072 at 1160.486 kb on - strand, within Psest_1072 at 1160.486 kb on - strand, within Psest_1072 at 1160.486 kb on - strand, within Psest_1072 at 1160.672 kb on + strand, within Psest_1072 at 1161.073 kb on + strand, within Psest_1072 at 1161.073 kb on + strand, within Psest_1072 at 1161.073 kb on + strand, within Psest_1072 at 1161.073 kb on + strand, within Psest_1072 at 1161.074 kb on - strand, within Psest_1072 at 1161.074 kb on - strand, within Psest_1072 at 1161.074 kb on - strand, within Psest_1072 at 1161.074 kb on - strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.082 kb on + strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.083 kb on - strand, within Psest_1072 at 1161.253 kb on + strand, within Psest_1072 at 1161.254 kb on - strand, within Psest_1072
Per-strain Table
Position Strand Gene LocusTag Fraction Def Polymyxin B sulfate 0.00008 mg/ml remove 1,157,976 - +1.0 1,158,125 + +2.7 1,158,125 + +1.2 1,158,126 - +1.4 1,158,238 + +0.9 1,158,246 + -0.2 1,158,246 + +1.1 1,158,246 + -0.1 1,158,247 - +1.2 1,158,247 - +3.0 1,158,247 - +1.6 1,158,247 - +0.6 1,158,247 - +0.9 1,158,447 + Psest_1070 0.51 +0.2 1,158,447 + Psest_1070 0.51 +0.7 1,158,448 - Psest_1070 0.51 +0.6 1,158,493 - Psest_1070 0.62 +1.0 1,158,567 - Psest_1070 0.81 +0.9 1,158,694 + +1.7 1,158,695 - +1.3 1,158,695 - +1.2 1,158,695 - +1.1 1,158,695 - +2.0 1,158,695 - +0.8 1,158,748 - +2.2 1,158,792 + +1.4 1,158,793 - -0.3 1,158,793 - -0.7 1,158,978 + Psest_1071 0.15 +0.2 1,158,978 + Psest_1071 0.15 +2.0 1,158,979 - Psest_1071 0.15 -0.3 1,159,040 + Psest_1071 0.19 +1.0 1,159,041 - Psest_1071 0.19 +0.5 1,159,041 - Psest_1071 0.19 +1.6 1,159,041 - Psest_1071 0.19 +0.4 1,159,272 + Psest_1071 0.34 +0.0 1,159,272 + Psest_1071 0.34 -1.5 1,159,275 + Psest_1071 0.35 +0.8 1,159,275 + Psest_1071 0.35 +1.6 1,159,275 + Psest_1071 0.35 -0.3 1,159,276 - Psest_1071 0.35 -0.4 1,159,276 - Psest_1071 0.35 +1.5 1,159,403 + Psest_1071 0.43 +2.0 1,159,767 + Psest_1071 0.67 +1.7 1,159,767 + Psest_1071 0.67 +2.0 1,160,085 + Psest_1071 0.89 +0.3 1,160,086 - Psest_1071 0.89 +0.4 1,160,086 - Psest_1071 0.89 -0.2 1,160,086 - Psest_1071 0.89 +0.6 1,160,086 - Psest_1071 0.89 +0.2 1,160,086 - Psest_1071 0.89 -0.6 1,160,345 - +1.4 1,160,383 + +0.6 1,160,383 + +0.3 1,160,383 + +0.9 1,160,422 + -0.3 1,160,440 + +0.0 1,160,440 + +0.4 1,160,441 - +0.8 1,160,485 + Psest_1072 0.11 -1.2 1,160,485 + Psest_1072 0.11 +0.1 1,160,485 + Psest_1072 0.11 +1.2 1,160,485 + Psest_1072 0.11 +0.1 1,160,485 + Psest_1072 0.11 +0.4 1,160,485 + Psest_1072 0.11 -0.2 1,160,485 + Psest_1072 0.11 +0.6 1,160,485 + Psest_1072 0.11 +0.7 1,160,485 + Psest_1072 0.11 +0.4 1,160,486 - Psest_1072 0.11 +1.2 1,160,486 - Psest_1072 0.11 +1.2 1,160,486 - Psest_1072 0.11 +0.2 1,160,486 - Psest_1072 0.11 +0.9 1,160,672 + Psest_1072 0.25 +0.6 1,161,073 + Psest_1072 0.54 +1.5 1,161,073 + Psest_1072 0.54 -0.7 1,161,073 + Psest_1072 0.54 +0.3 1,161,073 + Psest_1072 0.54 +0.4 1,161,074 - Psest_1072 0.54 +0.1 1,161,074 - Psest_1072 0.54 +0.3 1,161,074 - Psest_1072 0.54 -0.7 1,161,074 - Psest_1072 0.54 +0.7 1,161,082 + Psest_1072 0.55 -0.2 1,161,082 + Psest_1072 0.55 +0.6 1,161,082 + Psest_1072 0.55 +0.2 1,161,082 + Psest_1072 0.55 -0.9 1,161,082 + Psest_1072 0.55 +0.4 1,161,082 + Psest_1072 0.55 -0.1 1,161,082 + Psest_1072 0.55 +0.9 1,161,082 + Psest_1072 0.55 +0.4 1,161,082 + Psest_1072 0.55 +1.0 1,161,082 + Psest_1072 0.55 +0.3 1,161,082 + Psest_1072 0.55 -1.1 1,161,082 + Psest_1072 0.55 -0.4 1,161,083 - Psest_1072 0.55 -0.4 1,161,083 - Psest_1072 0.55 +0.7 1,161,083 - Psest_1072 0.55 -1.0 1,161,083 - Psest_1072 0.55 +0.7 1,161,083 - Psest_1072 0.55 -0.1 1,161,083 - Psest_1072 0.55 -0.7 1,161,083 - Psest_1072 0.55 -0.5 1,161,083 - Psest_1072 0.55 -1.1 1,161,083 - Psest_1072 0.55 +0.2 1,161,083 - Psest_1072 0.55 +0.4 1,161,083 - Psest_1072 0.55 +0.5 1,161,083 - Psest_1072 0.55 +1.5 1,161,083 - Psest_1072 0.55 -1.9 1,161,083 - Psest_1072 0.55 -0.6 1,161,083 - Psest_1072 0.55 +0.2 1,161,253 + Psest_1072 0.67 +0.4 1,161,254 - Psest_1072 0.67 +1.1
Or see this region's nucleotide sequence