Experiment: R2A with 2-pentyl-furan 32.0526 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS16515 and ABIE51_RS16520 are separated by 458 nucleotides ABIE51_RS16520 and ABIE51_RS16525 overlap by 4 nucleotides ABIE51_RS16525 and ABIE51_RS16530 are separated by 71 nucleotides ABIE51_RS16530 and ABIE51_RS16535 are separated by 97 nucleotides
ABIE51_RS16515: ABIE51_RS16515 - response regulator transcription factor, at 3,591,973 to 3,592,653
_RS16515
ABIE51_RS16520: ABIE51_RS16520 - 30S ribosomal protein S6--L-glutamate ligase, at 3,593,112 to 3,594,017
_RS16520
ABIE51_RS16525: ABIE51_RS16525 - RimK/LysX family protein, at 3,594,014 to 3,594,529
_RS16525
ABIE51_RS16530: ABIE51_RS16530 - tRNA-Gly, at 3,594,601 to 3,594,674
_RS16530
ABIE51_RS16535: ABIE51_RS16535 - autotransporter domain-containing protein, at 3,594,772 to 3,596,607
_RS16535
Position (kb)
3593
3594
3595 Strain fitness (log2 ratio)
-2
-1
0
1 at 3592.669 kb on + strand at 3592.754 kb on - strand at 3592.902 kb on + strand at 3592.902 kb on + strand at 3592.902 kb on + strand at 3592.902 kb on + strand at 3592.919 kb on + strand at 3592.920 kb on - strand at 3592.920 kb on - strand at 3592.967 kb on + strand at 3592.978 kb on + strand at 3593.017 kb on + strand at 3593.017 kb on + strand at 3593.141 kb on + strand at 3593.142 kb on - strand at 3593.142 kb on - strand at 3593.164 kb on + strand at 3593.164 kb on + strand at 3593.164 kb on + strand at 3593.165 kb on - strand at 3593.165 kb on - strand at 3593.165 kb on - strand at 3593.165 kb on - strand at 3593.507 kb on - strand, within ABIE51_RS16520 at 3593.759 kb on + strand, within ABIE51_RS16520 at 3593.759 kb on + strand, within ABIE51_RS16520 at 3593.759 kb on + strand, within ABIE51_RS16520 at 3593.760 kb on - strand, within ABIE51_RS16520 at 3593.760 kb on - strand, within ABIE51_RS16520 at 3593.869 kb on + strand, within ABIE51_RS16520 at 3593.869 kb on + strand, within ABIE51_RS16520 at 3593.869 kb on + strand, within ABIE51_RS16520 at 3593.870 kb on - strand, within ABIE51_RS16520 at 3593.870 kb on - strand, within ABIE51_RS16520 at 3593.870 kb on - strand, within ABIE51_RS16520 at 3593.870 kb on - strand, within ABIE51_RS16520 at 3593.870 kb on - strand, within ABIE51_RS16520 at 3593.870 kb on - strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.905 kb on + strand, within ABIE51_RS16520 at 3593.906 kb on - strand, within ABIE51_RS16520 at 3593.906 kb on - strand, within ABIE51_RS16520 at 3593.906 kb on - strand, within ABIE51_RS16520 at 3593.906 kb on - strand, within ABIE51_RS16520 at 3593.906 kb on - strand, within ABIE51_RS16520 at 3593.936 kb on + strand at 3593.936 kb on + strand at 3593.937 kb on - strand at 3593.937 kb on - strand at 3593.980 kb on + strand at 3593.980 kb on + strand at 3593.980 kb on + strand at 3593.981 kb on - strand at 3593.981 kb on - strand at 3593.981 kb on - strand at 3594.385 kb on + strand, within ABIE51_RS16525 at 3594.386 kb on - strand, within ABIE51_RS16525 at 3594.491 kb on + strand at 3594.575 kb on + strand at 3594.575 kb on + strand at 3594.575 kb on + strand at 3594.576 kb on - strand at 3594.631 kb on + strand, within ABIE51_RS16530 at 3594.631 kb on + strand, within ABIE51_RS16530 at 3594.632 kb on - strand, within ABIE51_RS16530 at 3594.655 kb on + strand, within ABIE51_RS16530 at 3594.655 kb on + strand, within ABIE51_RS16530 at 3594.655 kb on + strand, within ABIE51_RS16530 at 3594.655 kb on + strand, within ABIE51_RS16530 at 3594.655 kb on + strand, within ABIE51_RS16530 at 3594.656 kb on - strand, within ABIE51_RS16530 at 3594.656 kb on - strand, within ABIE51_RS16530 at 3594.656 kb on - strand, within ABIE51_RS16530 at 3594.666 kb on + strand, within ABIE51_RS16530 at 3594.666 kb on + strand, within ABIE51_RS16530 at 3594.666 kb on + strand, within ABIE51_RS16530 at 3594.666 kb on + strand, within ABIE51_RS16530 at 3594.666 kb on + strand, within ABIE51_RS16530 at 3594.666 kb on + strand, within ABIE51_RS16530 at 3594.667 kb on - strand at 3594.667 kb on - strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.689 kb on + strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.690 kb on - strand at 3594.773 kb on + strand at 3594.773 kb on + strand at 3594.773 kb on + strand at 3594.774 kb on - strand at 3594.774 kb on - strand at 3594.774 kb on - strand at 3594.774 kb on - strand at 3594.774 kb on - strand at 3594.774 kb on - strand at 3594.903 kb on - strand at 3594.903 kb on - strand at 3594.915 kb on - strand at 3594.915 kb on - strand at 3594.915 kb on - strand at 3594.935 kb on + strand at 3594.936 kb on - strand at 3594.936 kb on - strand at 3594.936 kb on - strand at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.007 kb on + strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535 at 3595.008 kb on - strand, within ABIE51_RS16535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 32.0526 mM remove 3,592,669 + -0.9 3,592,754 - -1.3 3,592,902 + -0.8 3,592,902 + -0.3 3,592,902 + -0.2 3,592,902 + -0.2 3,592,919 + -0.9 3,592,920 - -0.6 3,592,920 - -0.9 3,592,967 + -1.2 3,592,978 + -0.6 3,593,017 + -0.0 3,593,017 + -0.4 3,593,141 + -0.8 3,593,142 - -0.2 3,593,142 - +0.4 3,593,164 + -1.2 3,593,164 + -1.8 3,593,164 + +0.6 3,593,165 - -1.0 3,593,165 - -2.0 3,593,165 - -1.7 3,593,165 - -1.2 3,593,507 - ABIE51_RS16520 0.44 -1.3 3,593,759 + ABIE51_RS16520 0.71 -0.4 3,593,759 + ABIE51_RS16520 0.71 -0.2 3,593,759 + ABIE51_RS16520 0.71 -1.0 3,593,760 - ABIE51_RS16520 0.72 -0.6 3,593,760 - ABIE51_RS16520 0.72 -1.3 3,593,869 + ABIE51_RS16520 0.84 -1.3 3,593,869 + ABIE51_RS16520 0.84 -1.2 3,593,869 + ABIE51_RS16520 0.84 -0.2 3,593,870 - ABIE51_RS16520 0.84 -1.2 3,593,870 - ABIE51_RS16520 0.84 -1.1 3,593,870 - ABIE51_RS16520 0.84 -1.1 3,593,870 - ABIE51_RS16520 0.84 -0.9 3,593,870 - ABIE51_RS16520 0.84 -0.8 3,593,870 - ABIE51_RS16520 0.84 -1.4 3,593,905 + ABIE51_RS16520 0.88 -0.7 3,593,905 + ABIE51_RS16520 0.88 -1.1 3,593,905 + ABIE51_RS16520 0.88 -1.7 3,593,905 + ABIE51_RS16520 0.88 -2.6 3,593,905 + ABIE51_RS16520 0.88 -0.7 3,593,905 + ABIE51_RS16520 0.88 -0.9 3,593,905 + ABIE51_RS16520 0.88 -0.6 3,593,905 + ABIE51_RS16520 0.88 -0.4 3,593,906 - ABIE51_RS16520 0.88 -0.7 3,593,906 - ABIE51_RS16520 0.88 -0.6 3,593,906 - ABIE51_RS16520 0.88 -1.7 3,593,906 - ABIE51_RS16520 0.88 -1.0 3,593,906 - ABIE51_RS16520 0.88 -0.9 3,593,936 + +0.0 3,593,936 + -1.3 3,593,937 - -1.2 3,593,937 - -1.1 3,593,980 + -1.2 3,593,980 + -0.8 3,593,980 + -1.0 3,593,981 - -1.5 3,593,981 - -0.8 3,593,981 - -0.2 3,594,385 + ABIE51_RS16525 0.72 -0.5 3,594,386 - ABIE51_RS16525 0.72 +0.4 3,594,491 + -0.9 3,594,575 + -0.5 3,594,575 + +0.6 3,594,575 + +0.5 3,594,576 - +0.4 3,594,631 + ABIE51_RS16530 0.41 +0.2 3,594,631 + ABIE51_RS16530 0.41 +0.4 3,594,632 - ABIE51_RS16530 0.42 +0.5 3,594,655 + ABIE51_RS16530 0.73 +0.3 3,594,655 + ABIE51_RS16530 0.73 -0.2 3,594,655 + ABIE51_RS16530 0.73 -0.1 3,594,655 + ABIE51_RS16530 0.73 +0.3 3,594,655 + ABIE51_RS16530 0.73 +0.3 3,594,656 - ABIE51_RS16530 0.74 -0.2 3,594,656 - ABIE51_RS16530 0.74 -0.0 3,594,656 - ABIE51_RS16530 0.74 +0.1 3,594,666 + ABIE51_RS16530 0.88 -1.6 3,594,666 + ABIE51_RS16530 0.88 -0.6 3,594,666 + ABIE51_RS16530 0.88 +1.4 3,594,666 + ABIE51_RS16530 0.88 -0.5 3,594,666 + ABIE51_RS16530 0.88 +0.0 3,594,666 + ABIE51_RS16530 0.88 -0.4 3,594,667 - -0.6 3,594,667 - -0.3 3,594,689 + -0.2 3,594,689 + -0.0 3,594,689 + -0.1 3,594,689 + +1.4 3,594,689 + -0.3 3,594,689 + +0.0 3,594,689 + +0.3 3,594,689 + -0.6 3,594,689 + +0.7 3,594,689 + +0.3 3,594,689 + -0.4 3,594,690 - -0.0 3,594,690 - -0.0 3,594,690 - -0.3 3,594,690 - -0.2 3,594,690 - -0.6 3,594,690 - +0.7 3,594,690 - -0.3 3,594,690 - -0.4 3,594,773 + +0.2 3,594,773 + -0.5 3,594,773 + +0.5 3,594,774 - +0.1 3,594,774 - +0.1 3,594,774 - -0.4 3,594,774 - +0.3 3,594,774 - -0.2 3,594,774 - +0.4 3,594,903 - +0.0 3,594,903 - +0.9 3,594,915 - -0.4 3,594,915 - +0.6 3,594,915 - +0.0 3,594,935 + -0.3 3,594,936 - -0.0 3,594,936 - +0.4 3,594,936 - +0.4 3,595,007 + ABIE51_RS16535 0.13 +0.4 3,595,007 + ABIE51_RS16535 0.13 +0.1 3,595,007 + ABIE51_RS16535 0.13 -0.4 3,595,007 + ABIE51_RS16535 0.13 +0.3 3,595,007 + ABIE51_RS16535 0.13 +0.1 3,595,007 + ABIE51_RS16535 0.13 -0.1 3,595,007 + ABIE51_RS16535 0.13 +0.2 3,595,008 - ABIE51_RS16535 0.13 -0.4 3,595,008 - ABIE51_RS16535 0.13 +0.4 3,595,008 - ABIE51_RS16535 0.13 +0.4 3,595,008 - ABIE51_RS16535 0.13 +0.2 3,595,008 - ABIE51_RS16535 0.13 +0.2 3,595,008 - ABIE51_RS16535 0.13 -0.2 3,595,008 - ABIE51_RS16535 0.13 +0.3
Or see this region's nucleotide sequence