Experiment: McCas_formate_complete 3-nitroxypropanol 0.00078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04710 and MMJJ_RS04715 are separated by 271 nucleotides MMJJ_RS04715 and MMJJ_RS04720 are separated by 49 nucleotides MMJJ_RS04720 and MMJJ_RS04725 are separated by 72 nucleotides MMJJ_RS04725 and MMJJ_RS04730 are separated by 49 nucleotides
MMJJ_RS04710: MMJJ_RS04710 - threonine synthase, at 882,793 to 884,007
_RS04710
MMJJ_RS04715: MMJJ_RS04715 - hypothetical protein, at 884,279 to 884,623
_RS04715
MMJJ_RS04720: MMJJ_RS04720 - IMP dehydrogenase, at 884,673 to 886,175
_RS04720
MMJJ_RS04725: MMJJ_RS04725 - branched-chain-amino-acid transaminase, at 886,248 to 887,111
_RS04725
MMJJ_RS04730: MMJJ_RS04730 - tyrosine decarboxylase MfnA, at 887,161 to 888,315
_RS04730
Position (kb) 884
885
886
887 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 884.062 kb on - strand at 884.063 kb on + strand at 884.089 kb on + strand at 884.089 kb on + strand at 884.091 kb on - strand at 884.151 kb on - strand at 884.157 kb on - strand at 884.177 kb on - strand at 884.177 kb on - strand at 884.182 kb on + strand at 884.182 kb on + strand at 884.184 kb on - strand at 884.184 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.190 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.222 kb on - strand at 884.222 kb on - strand at 884.222 kb on - strand at 884.222 kb on - strand at 884.239 kb on + strand at 884.280 kb on - strand at 884.287 kb on + strand at 884.300 kb on - strand at 884.300 kb on - strand at 884.300 kb on - strand at 884.300 kb on - strand at 884.303 kb on + strand at 884.325 kb on + strand, within MMJJ_RS04715 at 884.372 kb on - strand, within MMJJ_RS04715 at 884.425 kb on - strand, within MMJJ_RS04715 at 884.425 kb on - strand, within MMJJ_RS04715 at 884.425 kb on - strand, within MMJJ_RS04715 at 884.426 kb on + strand, within MMJJ_RS04715 at 884.426 kb on + strand, within MMJJ_RS04715 at 884.426 kb on + strand, within MMJJ_RS04715 at 884.481 kb on + strand, within MMJJ_RS04715 at 884.497 kb on - strand, within MMJJ_RS04715 at 884.497 kb on - strand, within MMJJ_RS04715 at 884.498 kb on + strand, within MMJJ_RS04715 at 884.498 kb on + strand, within MMJJ_RS04715 at 884.609 kb on + strand at 884.609 kb on + strand at 884.639 kb on + strand at 884.639 kb on + strand at 885.290 kb on - strand at 885.602 kb on + strand, within MMJJ_RS04720 at 885.980 kb on - strand, within MMJJ_RS04720 at 886.081 kb on - strand at 886.173 kb on - strand at 886.187 kb on + strand at 886.194 kb on + strand at 886.246 kb on - strand at 886.247 kb on + strand at 886.247 kb on + strand at 886.249 kb on - strand at 886.249 kb on - strand at 886.254 kb on - strand at 886.254 kb on - strand at 886.255 kb on + strand at 886.255 kb on + strand at 886.255 kb on + strand at 886.258 kb on - strand at 886.259 kb on + strand at 886.259 kb on + strand at 886.281 kb on - strand at 886.282 kb on + strand at 886.282 kb on + strand at 886.295 kb on - strand at 886.302 kb on - strand at 886.302 kb on - strand at 886.302 kb on - strand at 886.303 kb on + strand at 886.323 kb on - strand at 886.338 kb on + strand, within MMJJ_RS04725 at 886.338 kb on + strand, within MMJJ_RS04725 at 886.372 kb on + strand, within MMJJ_RS04725 at 886.395 kb on + strand, within MMJJ_RS04725 at 886.417 kb on + strand, within MMJJ_RS04725 at 886.443 kb on - strand, within MMJJ_RS04725 at 886.446 kb on + strand, within MMJJ_RS04725 at 886.451 kb on - strand, within MMJJ_RS04725 at 886.451 kb on - strand, within MMJJ_RS04725 at 886.452 kb on + strand, within MMJJ_RS04725 at 886.476 kb on - strand, within MMJJ_RS04725 at 886.477 kb on + strand, within MMJJ_RS04725 at 886.494 kb on + strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.557 kb on - strand, within MMJJ_RS04725 at 886.627 kb on - strand, within MMJJ_RS04725 at 886.659 kb on - strand, within MMJJ_RS04725 at 886.660 kb on + strand, within MMJJ_RS04725 at 886.660 kb on + strand, within MMJJ_RS04725 at 886.689 kb on - strand, within MMJJ_RS04725 at 886.698 kb on - strand, within MMJJ_RS04725 at 886.699 kb on + strand, within MMJJ_RS04725 at 886.704 kb on - strand, within MMJJ_RS04725 at 886.726 kb on + strand, within MMJJ_RS04725 at 886.726 kb on + strand, within MMJJ_RS04725 at 886.728 kb on + strand, within MMJJ_RS04725 at 886.728 kb on + strand, within MMJJ_RS04725 at 886.838 kb on - strand, within MMJJ_RS04725 at 886.838 kb on - strand, within MMJJ_RS04725 at 886.838 kb on - strand, within MMJJ_RS04725 at 886.842 kb on - strand, within MMJJ_RS04725 at 886.842 kb on - strand, within MMJJ_RS04725 at 886.843 kb on + strand, within MMJJ_RS04725 at 886.843 kb on + strand, within MMJJ_RS04725 at 886.843 kb on + strand, within MMJJ_RS04725 at 886.851 kb on - strand, within MMJJ_RS04725 at 886.851 kb on - strand, within MMJJ_RS04725 at 886.851 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.855 kb on - strand, within MMJJ_RS04725 at 886.856 kb on + strand, within MMJJ_RS04725 at 886.866 kb on - strand, within MMJJ_RS04725 at 886.867 kb on + strand, within MMJJ_RS04725 at 886.970 kb on - strand, within MMJJ_RS04725 at 887.138 kb on - strand at 887.139 kb on + strand at 887.147 kb on - strand at 887.147 kb on - strand at 887.148 kb on + strand at 887.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 3-nitroxypropanol 0.00078125 mM remove 884,062 - +1.2 884,063 + +0.5 884,089 + +0.8 884,089 + -1.5 884,091 - -0.4 884,151 - +3.4 884,157 - +0.5 884,177 - +0.2 884,177 - -0.0 884,182 + +0.9 884,182 + +0.4 884,184 - -1.6 884,184 - -3.9 884,188 - -2.2 884,188 - +1.5 884,188 - -1.0 884,188 - +1.1 884,189 + +1.0 884,189 + -0.3 884,189 + +0.0 884,189 + -0.3 884,189 + -0.2 884,189 + -0.7 884,189 + -0.1 884,189 + -0.6 884,189 + -1.2 884,189 + +0.5 884,189 + +1.8 884,190 - +0.1 884,218 - -2.8 884,218 - -1.1 884,218 - +0.7 884,218 - +4.1 884,218 - -0.1 884,222 - -1.5 884,222 - +1.0 884,222 - -0.7 884,222 - -0.1 884,239 + +1.4 884,280 - -1.4 884,287 + +1.4 884,300 - -0.8 884,300 - +0.7 884,300 - +2.2 884,300 - +0.4 884,303 + -0.5 884,325 + MMJJ_RS04715 0.13 +0.3 884,372 - MMJJ_RS04715 0.27 -0.7 884,425 - MMJJ_RS04715 0.42 +0.4 884,425 - MMJJ_RS04715 0.42 -0.8 884,425 - MMJJ_RS04715 0.42 +0.9 884,426 + MMJJ_RS04715 0.43 -0.8 884,426 + MMJJ_RS04715 0.43 -0.9 884,426 + MMJJ_RS04715 0.43 +3.5 884,481 + MMJJ_RS04715 0.59 -0.2 884,497 - MMJJ_RS04715 0.63 +0.8 884,497 - MMJJ_RS04715 0.63 -0.4 884,498 + MMJJ_RS04715 0.63 -0.0 884,498 + MMJJ_RS04715 0.63 -1.1 884,609 + +2.0 884,609 + -0.9 884,639 + +0.4 884,639 + +2.3 885,290 - +0.4 885,602 + MMJJ_RS04720 0.62 -1.0 885,980 - MMJJ_RS04720 0.87 -1.8 886,081 - +1.0 886,173 - +1.7 886,187 + -0.5 886,194 + -0.0 886,246 - +0.6 886,247 + -0.1 886,247 + +0.1 886,249 - -0.3 886,249 - -0.8 886,254 - -0.4 886,254 - -0.4 886,255 + +1.0 886,255 + +1.4 886,255 + +1.8 886,258 - -1.3 886,259 + +0.2 886,259 + +0.6 886,281 - -0.2 886,282 + -0.5 886,282 + -0.1 886,295 - +2.6 886,302 - +0.6 886,302 - +2.3 886,302 - +1.1 886,303 + +0.5 886,323 - +0.7 886,338 + MMJJ_RS04725 0.10 +0.1 886,338 + MMJJ_RS04725 0.10 +0.6 886,372 + MMJJ_RS04725 0.14 -2.0 886,395 + MMJJ_RS04725 0.17 +1.2 886,417 + MMJJ_RS04725 0.20 +1.4 886,443 - MMJJ_RS04725 0.23 -0.2 886,446 + MMJJ_RS04725 0.23 -0.0 886,451 - MMJJ_RS04725 0.23 +0.8 886,451 - MMJJ_RS04725 0.23 +0.7 886,452 + MMJJ_RS04725 0.24 +0.2 886,476 - MMJJ_RS04725 0.26 -0.8 886,477 + MMJJ_RS04725 0.27 -0.5 886,494 + MMJJ_RS04725 0.28 +0.0 886,557 - MMJJ_RS04725 0.36 +0.5 886,557 - MMJJ_RS04725 0.36 -1.2 886,557 - MMJJ_RS04725 0.36 +0.6 886,557 - MMJJ_RS04725 0.36 +0.5 886,627 - MMJJ_RS04725 0.44 +0.4 886,659 - MMJJ_RS04725 0.48 +0.3 886,660 + MMJJ_RS04725 0.48 +0.1 886,660 + MMJJ_RS04725 0.48 -0.7 886,689 - MMJJ_RS04725 0.51 +0.6 886,698 - MMJJ_RS04725 0.52 -0.4 886,699 + MMJJ_RS04725 0.52 -1.6 886,704 - MMJJ_RS04725 0.53 +1.0 886,726 + MMJJ_RS04725 0.55 -0.9 886,726 + MMJJ_RS04725 0.55 -1.2 886,728 + MMJJ_RS04725 0.56 -2.3 886,728 + MMJJ_RS04725 0.56 -0.7 886,838 - MMJJ_RS04725 0.68 +4.4 886,838 - MMJJ_RS04725 0.68 -0.8 886,838 - MMJJ_RS04725 0.68 -0.1 886,842 - MMJJ_RS04725 0.69 +1.3 886,842 - MMJJ_RS04725 0.69 -1.0 886,843 + MMJJ_RS04725 0.69 +1.6 886,843 + MMJJ_RS04725 0.69 -0.6 886,843 + MMJJ_RS04725 0.69 +1.7 886,851 - MMJJ_RS04725 0.70 +1.7 886,851 - MMJJ_RS04725 0.70 +1.3 886,851 - MMJJ_RS04725 0.70 -0.3 886,855 - MMJJ_RS04725 0.70 +0.2 886,855 - MMJJ_RS04725 0.70 +1.0 886,855 - MMJJ_RS04725 0.70 -0.9 886,855 - MMJJ_RS04725 0.70 +1.0 886,855 - MMJJ_RS04725 0.70 +0.2 886,856 + MMJJ_RS04725 0.70 +1.9 886,866 - MMJJ_RS04725 0.72 +1.2 886,867 + MMJJ_RS04725 0.72 +0.0 886,970 - MMJJ_RS04725 0.84 +0.5 887,138 - +1.2 887,139 + +2.8 887,147 - -0.9 887,147 - +0.5 887,148 + -0.7 887,156 + -1.0
Or see this region's nucleotide sequence