Experiment: McCas_formate_complete sulfate 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03405 and MMJJ_RS03410 overlap by 8 nucleotides MMJJ_RS03410 and MMJJ_RS03415 are separated by 3 nucleotides MMJJ_RS03415 and MMJJ_RS03420 overlap by 8 nucleotides MMJJ_RS03420 and MMJJ_RS03425 are separated by 0 nucleotides MMJJ_RS03425 and MMJJ_RS03430 are separated by 220 nucleotides
MMJJ_RS03405: MMJJ_RS03405 - hypothetical protein, at 640,901 to 642,241
_RS03405
MMJJ_RS03410: MMJJ_RS03410 - hypothetical protein, at 642,234 to 642,650
_RS03410
MMJJ_RS03415: MMJJ_RS03415 - hypothetical protein, at 642,654 to 643,091
_RS03415
MMJJ_RS03420: MMJJ_RS03420 - hypothetical protein, at 643,084 to 643,359
_RS03420
MMJJ_RS03425: MMJJ_RS03425 - DUF2080 family transposase-associated protein, at 643,360 to 643,485
_RS03425
MMJJ_RS03430: MMJJ_RS03430 - winged helix-turn-helix domain-containing protein, at 643,706 to 644,071
_RS03430
Position (kb)
642
643
644 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 641.697 kb on + strand at 641.728 kb on + strand, within MMJJ_RS03405 at 641.735 kb on + strand, within MMJJ_RS03405 at 641.735 kb on + strand, within MMJJ_RS03405 at 641.737 kb on + strand, within MMJJ_RS03405 at 641.777 kb on + strand, within MMJJ_RS03405 at 641.789 kb on - strand, within MMJJ_RS03405 at 641.879 kb on - strand, within MMJJ_RS03405 at 641.903 kb on + strand, within MMJJ_RS03405 at 641.907 kb on - strand, within MMJJ_RS03405 at 642.103 kb on + strand, within MMJJ_RS03405 at 642.161 kb on - strand at 642.208 kb on + strand at 642.238 kb on + strand at 642.238 kb on + strand at 642.285 kb on - strand, within MMJJ_RS03410 at 642.329 kb on - strand, within MMJJ_RS03410 at 642.368 kb on - strand, within MMJJ_RS03410 at 642.420 kb on - strand, within MMJJ_RS03410 at 642.508 kb on - strand, within MMJJ_RS03410 at 642.540 kb on - strand, within MMJJ_RS03410 at 642.595 kb on - strand, within MMJJ_RS03410 at 642.656 kb on + strand at 642.734 kb on + strand, within MMJJ_RS03415 at 642.741 kb on - strand, within MMJJ_RS03415 at 642.795 kb on + strand, within MMJJ_RS03415 at 642.821 kb on - strand, within MMJJ_RS03415 at 642.821 kb on - strand, within MMJJ_RS03415 at 642.873 kb on - strand, within MMJJ_RS03415 at 642.891 kb on - strand, within MMJJ_RS03415 at 642.899 kb on + strand, within MMJJ_RS03415 at 642.899 kb on + strand, within MMJJ_RS03415 at 642.951 kb on - strand, within MMJJ_RS03415 at 642.951 kb on - strand, within MMJJ_RS03415 at 642.951 kb on - strand, within MMJJ_RS03415 at 642.957 kb on - strand, within MMJJ_RS03415 at 642.958 kb on + strand, within MMJJ_RS03415 at 642.988 kb on - strand, within MMJJ_RS03415 at 643.065 kb on + strand at 643.071 kb on + strand at 643.071 kb on + strand at 643.108 kb on - strand at 643.116 kb on + strand, within MMJJ_RS03420 at 643.116 kb on + strand, within MMJJ_RS03420 at 643.116 kb on + strand, within MMJJ_RS03420 at 643.133 kb on - strand, within MMJJ_RS03420 at 643.134 kb on + strand, within MMJJ_RS03420 at 643.134 kb on + strand, within MMJJ_RS03420 at 643.188 kb on + strand, within MMJJ_RS03420 at 643.213 kb on - strand, within MMJJ_RS03420 at 643.214 kb on + strand, within MMJJ_RS03420 at 643.214 kb on + strand, within MMJJ_RS03420 at 643.214 kb on + strand, within MMJJ_RS03420 at 643.217 kb on - strand, within MMJJ_RS03420 at 643.217 kb on - strand, within MMJJ_RS03420 at 643.218 kb on + strand, within MMJJ_RS03420 at 643.232 kb on + strand, within MMJJ_RS03420 at 643.238 kb on - strand, within MMJJ_RS03420 at 643.239 kb on + strand, within MMJJ_RS03420 at 643.279 kb on - strand, within MMJJ_RS03420 at 643.280 kb on + strand, within MMJJ_RS03420 at 643.312 kb on - strand, within MMJJ_RS03420 at 643.313 kb on + strand, within MMJJ_RS03420 at 643.313 kb on + strand, within MMJJ_RS03420 at 643.317 kb on - strand, within MMJJ_RS03420 at 643.317 kb on - strand, within MMJJ_RS03420 at 643.317 kb on - strand, within MMJJ_RS03420 at 643.317 kb on - strand, within MMJJ_RS03420 at 643.318 kb on + strand, within MMJJ_RS03420 at 643.319 kb on - strand, within MMJJ_RS03420 at 643.320 kb on + strand, within MMJJ_RS03420 at 643.320 kb on + strand, within MMJJ_RS03420 at 643.362 kb on + strand at 643.393 kb on - strand, within MMJJ_RS03425 at 643.395 kb on - strand, within MMJJ_RS03425 at 643.404 kb on - strand, within MMJJ_RS03425 at 643.405 kb on + strand, within MMJJ_RS03425 at 643.466 kb on + strand, within MMJJ_RS03425 at 643.591 kb on - strand at 643.591 kb on - strand at 643.591 kb on - strand at 643.591 kb on - strand at 643.592 kb on + strand at 643.592 kb on + strand at 643.592 kb on + strand at 643.592 kb on + strand at 643.661 kb on - strand at 643.667 kb on + strand at 643.682 kb on - strand at 643.779 kb on + strand, within MMJJ_RS03430 at 643.835 kb on + strand at 643.845 kb on - strand, within MMJJ_RS03430 at 643.863 kb on - strand, within MMJJ_RS03430 at 643.906 kb on - strand, within MMJJ_RS03430 at 643.918 kb on - strand, within MMJJ_RS03430 at 643.954 kb on + strand, within MMJJ_RS03430 at 643.956 kb on + strand at 644.001 kb on - strand, within MMJJ_RS03430 at 644.001 kb on - strand, within MMJJ_RS03430 at 644.020 kb on - strand, within MMJJ_RS03430 at 644.020 kb on - strand, within MMJJ_RS03430 at 644.021 kb on + strand, within MMJJ_RS03430 at 644.045 kb on + strand at 644.069 kb on - strand at 644.083 kb on + strand at 644.083 kb on + strand at 644.085 kb on - strand at 644.085 kb on - strand at 644.090 kb on + strand at 644.090 kb on + strand at 644.091 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sulfate 125 mM remove 641,697 + +1.6 641,728 + MMJJ_RS03405 0.62 -0.2 641,735 + MMJJ_RS03405 0.62 -3.0 641,735 + MMJJ_RS03405 0.62 +2.7 641,737 + MMJJ_RS03405 0.62 -2.5 641,777 + MMJJ_RS03405 0.65 -2.3 641,789 - MMJJ_RS03405 0.66 -2.1 641,879 - MMJJ_RS03405 0.73 -1.5 641,903 + MMJJ_RS03405 0.75 +1.5 641,907 - MMJJ_RS03405 0.75 -0.7 642,103 + MMJJ_RS03405 0.90 +0.6 642,161 - +0.3 642,208 + -3.1 642,238 + +0.9 642,238 + -1.9 642,285 - MMJJ_RS03410 0.12 +1.5 642,329 - MMJJ_RS03410 0.23 +0.2 642,368 - MMJJ_RS03410 0.32 -1.1 642,420 - MMJJ_RS03410 0.45 +2.5 642,508 - MMJJ_RS03410 0.66 +1.7 642,540 - MMJJ_RS03410 0.73 +2.7 642,595 - MMJJ_RS03410 0.87 -1.2 642,656 + +1.2 642,734 + MMJJ_RS03415 0.18 -2.4 642,741 - MMJJ_RS03415 0.20 -2.5 642,795 + MMJJ_RS03415 0.32 -0.0 642,821 - MMJJ_RS03415 0.38 -1.8 642,821 - MMJJ_RS03415 0.38 -3.0 642,873 - MMJJ_RS03415 0.50 -4.0 642,891 - MMJJ_RS03415 0.54 +2.5 642,899 + MMJJ_RS03415 0.56 +1.5 642,899 + MMJJ_RS03415 0.56 -4.9 642,951 - MMJJ_RS03415 0.68 +0.4 642,951 - MMJJ_RS03415 0.68 -1.4 642,951 - MMJJ_RS03415 0.68 -2.1 642,957 - MMJJ_RS03415 0.69 +0.3 642,958 + MMJJ_RS03415 0.69 -5.4 642,988 - MMJJ_RS03415 0.76 -3.4 643,065 + +1.6 643,071 + -1.5 643,071 + -3.8 643,108 - +0.2 643,116 + MMJJ_RS03420 0.12 +1.4 643,116 + MMJJ_RS03420 0.12 -2.1 643,116 + MMJJ_RS03420 0.12 -1.8 643,133 - MMJJ_RS03420 0.18 -3.0 643,134 + MMJJ_RS03420 0.18 -2.0 643,134 + MMJJ_RS03420 0.18 +0.2 643,188 + MMJJ_RS03420 0.38 -1.0 643,213 - MMJJ_RS03420 0.47 -0.1 643,214 + MMJJ_RS03420 0.47 +2.2 643,214 + MMJJ_RS03420 0.47 -2.3 643,214 + MMJJ_RS03420 0.47 -1.2 643,217 - MMJJ_RS03420 0.48 +1.9 643,217 - MMJJ_RS03420 0.48 +0.2 643,218 + MMJJ_RS03420 0.49 +0.8 643,232 + MMJJ_RS03420 0.54 +0.1 643,238 - MMJJ_RS03420 0.56 -2.8 643,239 + MMJJ_RS03420 0.56 +1.6 643,279 - MMJJ_RS03420 0.71 +1.1 643,280 + MMJJ_RS03420 0.71 -0.5 643,312 - MMJJ_RS03420 0.83 -1.6 643,313 + MMJJ_RS03420 0.83 -0.2 643,313 + MMJJ_RS03420 0.83 -3.3 643,317 - MMJJ_RS03420 0.84 -0.5 643,317 - MMJJ_RS03420 0.84 -1.2 643,317 - MMJJ_RS03420 0.84 +0.3 643,317 - MMJJ_RS03420 0.84 +0.2 643,318 + MMJJ_RS03420 0.85 +0.9 643,319 - MMJJ_RS03420 0.85 +0.6 643,320 + MMJJ_RS03420 0.86 -1.6 643,320 + MMJJ_RS03420 0.86 -1.3 643,362 + +0.9 643,393 - MMJJ_RS03425 0.26 -2.7 643,395 - MMJJ_RS03425 0.28 +1.7 643,404 - MMJJ_RS03425 0.35 -0.2 643,405 + MMJJ_RS03425 0.36 -1.7 643,466 + MMJJ_RS03425 0.84 -4.0 643,591 - +0.2 643,591 - +0.0 643,591 - +2.3 643,591 - -0.1 643,592 + +2.8 643,592 + -1.5 643,592 + -1.9 643,592 + -2.0 643,661 - -2.4 643,667 + +1.0 643,682 - -1.9 643,779 + MMJJ_RS03430 0.20 -0.6 643,835 + +2.6 643,845 - MMJJ_RS03430 0.38 -1.1 643,863 - MMJJ_RS03430 0.43 -1.6 643,906 - MMJJ_RS03430 0.55 -2.3 643,918 - MMJJ_RS03430 0.58 -0.6 643,954 + MMJJ_RS03430 0.68 +1.0 643,956 + +1.0 644,001 - MMJJ_RS03430 0.81 -3.8 644,001 - MMJJ_RS03430 0.81 -0.2 644,020 - MMJJ_RS03430 0.86 +1.8 644,020 - MMJJ_RS03430 0.86 +0.4 644,021 + MMJJ_RS03430 0.86 +1.2 644,045 + -0.6 644,069 - -4.7 644,083 + -0.9 644,083 + +0.0 644,085 - -0.3 644,085 - -3.2 644,090 + -3.1 644,090 + +1.9 644,091 - -4.1
Or see this region's nucleotide sequence