Experiment: McCas_formate_complete sulfate 125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02970 and MMJJ_RS02975 are separated by 38 nucleotides MMJJ_RS02975 and MMJJ_RS02980 are separated by 18 nucleotides MMJJ_RS02980 and MMJJ_RS02985 are separated by 170 nucleotides
MMJJ_RS02970: MMJJ_RS02970 - DUF378 domain-containing protein, at 554,163 to 554,411
_RS02970
MMJJ_RS02975: MMJJ_RS02975 - NAD(P)H-dependent oxidoreductase, at 554,450 to 554,944
_RS02975
MMJJ_RS02980: MMJJ_RS02980 - TatD family hydrolase, at 554,963 to 555,724
_RS02980
MMJJ_RS02985: MMJJ_RS02985 - MBL fold metallo-hydrolase, at 555,895 to 557,196
_RS02985
Position (kb) 554
555
556 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 554.009 kb on - strand at 554.009 kb on - strand at 554.010 kb on + strand at 554.010 kb on + strand at 554.010 kb on + strand at 554.011 kb on - strand at 554.023 kb on - strand at 554.024 kb on + strand at 554.095 kb on - strand at 554.095 kb on - strand at 554.095 kb on - strand at 554.096 kb on + strand at 554.096 kb on + strand at 554.097 kb on - strand at 554.098 kb on + strand at 554.116 kb on + strand at 554.120 kb on - strand at 554.121 kb on + strand at 554.121 kb on + strand at 554.121 kb on + strand at 554.121 kb on + strand at 554.122 kb on - strand at 554.123 kb on + strand at 554.147 kb on + strand at 554.147 kb on + strand at 554.160 kb on + strand at 554.181 kb on + strand at 554.242 kb on - strand, within MMJJ_RS02970 at 554.246 kb on - strand, within MMJJ_RS02970 at 554.247 kb on + strand, within MMJJ_RS02970 at 554.247 kb on + strand, within MMJJ_RS02970 at 554.260 kb on - strand, within MMJJ_RS02970 at 554.273 kb on - strand, within MMJJ_RS02970 at 554.273 kb on - strand, within MMJJ_RS02970 at 554.274 kb on + strand, within MMJJ_RS02970 at 554.297 kb on - strand, within MMJJ_RS02970 at 554.297 kb on - strand, within MMJJ_RS02970 at 554.297 kb on - strand, within MMJJ_RS02970 at 554.297 kb on - strand, within MMJJ_RS02970 at 554.297 kb on - strand, within MMJJ_RS02970 at 554.298 kb on + strand, within MMJJ_RS02970 at 554.298 kb on + strand, within MMJJ_RS02970 at 554.298 kb on + strand, within MMJJ_RS02970 at 554.300 kb on - strand, within MMJJ_RS02970 at 554.302 kb on - strand, within MMJJ_RS02970 at 554.302 kb on - strand, within MMJJ_RS02970 at 554.303 kb on + strand, within MMJJ_RS02970 at 554.303 kb on + strand, within MMJJ_RS02970 at 554.303 kb on + strand, within MMJJ_RS02970 at 554.303 kb on + strand, within MMJJ_RS02970 at 554.303 kb on + strand, within MMJJ_RS02970 at 554.318 kb on + strand, within MMJJ_RS02970 at 554.321 kb on + strand, within MMJJ_RS02970 at 554.353 kb on - strand, within MMJJ_RS02970 at 554.353 kb on - strand, within MMJJ_RS02970 at 554.354 kb on + strand, within MMJJ_RS02970 at 554.354 kb on + strand, within MMJJ_RS02970 at 554.354 kb on + strand, within MMJJ_RS02970 at 554.355 kb on - strand, within MMJJ_RS02970 at 554.356 kb on + strand, within MMJJ_RS02970 at 554.357 kb on - strand, within MMJJ_RS02970 at 554.357 kb on - strand, within MMJJ_RS02970 at 554.357 kb on - strand, within MMJJ_RS02970 at 554.358 kb on + strand, within MMJJ_RS02970 at 554.358 kb on + strand, within MMJJ_RS02970 at 554.372 kb on + strand, within MMJJ_RS02970 at 554.380 kb on - strand, within MMJJ_RS02970 at 554.381 kb on + strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.382 kb on - strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.383 kb on + strand, within MMJJ_RS02970 at 554.392 kb on + strand at 554.392 kb on + strand at 554.392 kb on + strand at 554.395 kb on + strand at 554.427 kb on + strand at 554.469 kb on - strand at 554.476 kb on - strand at 554.477 kb on + strand at 554.477 kb on + strand at 554.477 kb on + strand at 554.477 kb on + strand at 554.477 kb on + strand at 554.480 kb on - strand at 554.496 kb on - strand at 554.497 kb on + strand at 554.522 kb on - strand, within MMJJ_RS02975 at 554.522 kb on - strand, within MMJJ_RS02975 at 554.531 kb on - strand, within MMJJ_RS02975 at 554.532 kb on + strand, within MMJJ_RS02975 at 554.532 kb on + strand, within MMJJ_RS02975 at 554.551 kb on - strand, within MMJJ_RS02975 at 554.594 kb on + strand, within MMJJ_RS02975 at 554.594 kb on + strand, within MMJJ_RS02975 at 554.653 kb on + strand, within MMJJ_RS02975 at 554.713 kb on - strand, within MMJJ_RS02975 at 554.718 kb on + strand, within MMJJ_RS02975 at 554.888 kb on - strand, within MMJJ_RS02975 at 554.888 kb on - strand, within MMJJ_RS02975 at 554.889 kb on + strand, within MMJJ_RS02975 at 554.892 kb on - strand, within MMJJ_RS02975 at 554.892 kb on - strand, within MMJJ_RS02975 at 554.892 kb on - strand, within MMJJ_RS02975 at 554.892 kb on - strand, within MMJJ_RS02975 at 554.893 kb on + strand, within MMJJ_RS02975 at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.920 kb on + strand at 554.920 kb on + strand at 554.920 kb on + strand at 554.920 kb on + strand at 554.922 kb on + strand at 554.944 kb on - strand at 554.945 kb on + strand at 554.945 kb on + strand at 554.946 kb on - strand at 554.946 kb on - strand at 554.946 kb on - strand at 554.946 kb on - strand at 554.947 kb on + strand at 554.947 kb on + strand at 554.960 kb on - strand at 554.960 kb on - strand at 554.965 kb on + strand at 554.965 kb on + strand at 554.968 kb on - strand at 554.968 kb on - strand at 554.975 kb on + strand at 554.992 kb on - strand at 554.992 kb on - strand at 554.992 kb on - strand at 554.995 kb on - strand at 554.995 kb on - strand at 555.011 kb on - strand at 555.011 kb on - strand at 555.012 kb on + strand at 555.012 kb on + strand at 555.041 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.094 kb on - strand, within MMJJ_RS02980 at 555.100 kb on - strand, within MMJJ_RS02980 at 555.105 kb on + strand, within MMJJ_RS02980 at 555.138 kb on + strand, within MMJJ_RS02980 at 555.182 kb on + strand, within MMJJ_RS02980 at 555.213 kb on + strand, within MMJJ_RS02980 at 555.243 kb on + strand, within MMJJ_RS02980 at 555.243 kb on + strand, within MMJJ_RS02980 at 555.326 kb on + strand, within MMJJ_RS02980 at 555.344 kb on - strand, within MMJJ_RS02980 at 555.344 kb on - strand, within MMJJ_RS02980 at 555.378 kb on + strand, within MMJJ_RS02980 at 555.465 kb on - strand, within MMJJ_RS02980 at 555.523 kb on - strand, within MMJJ_RS02980 at 555.523 kb on - strand, within MMJJ_RS02980 at 555.524 kb on + strand, within MMJJ_RS02980 at 555.647 kb on + strand, within MMJJ_RS02980 at 555.760 kb on - strand at 555.761 kb on + strand at 555.791 kb on + strand at 555.794 kb on - strand at 555.803 kb on + strand at 555.812 kb on + strand at 555.844 kb on - strand at 555.844 kb on - strand at 555.847 kb on + strand at 555.847 kb on + strand at 555.847 kb on + strand at 555.849 kb on + strand at 555.855 kb on - strand at 555.855 kb on - strand at 555.856 kb on + strand at 555.863 kb on + strand at 555.863 kb on + strand at 555.872 kb on - strand at 555.872 kb on - strand at 555.872 kb on - strand at 555.873 kb on + strand at 555.944 kb on - strand at 555.975 kb on + strand at 556.029 kb on - strand, within MMJJ_RS02985 at 556.110 kb on - strand, within MMJJ_RS02985 at 556.110 kb on - strand, within MMJJ_RS02985 at 556.111 kb on + strand, within MMJJ_RS02985 at 556.126 kb on - strand, within MMJJ_RS02985 at 556.126 kb on - strand, within MMJJ_RS02985 at 556.127 kb on + strand, within MMJJ_RS02985 at 556.233 kb on - strand, within MMJJ_RS02985 at 556.261 kb on + strand, within MMJJ_RS02985 at 556.264 kb on - strand, within MMJJ_RS02985 at 556.347 kb on + strand, within MMJJ_RS02985 at 556.347 kb on + strand, within MMJJ_RS02985 at 556.373 kb on + strand, within MMJJ_RS02985 at 556.409 kb on - strand, within MMJJ_RS02985 at 556.471 kb on - strand, within MMJJ_RS02985 at 556.471 kb on - strand, within MMJJ_RS02985 at 556.471 kb on - strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.495 kb on + strand, within MMJJ_RS02985 at 556.562 kb on - strand, within MMJJ_RS02985 at 556.563 kb on + strand, within MMJJ_RS02985 at 556.607 kb on - strand, within MMJJ_RS02985 at 556.610 kb on - strand, within MMJJ_RS02985 at 556.611 kb on + strand, within MMJJ_RS02985 at 556.673 kb on - strand, within MMJJ_RS02985 at 556.673 kb on - strand, within MMJJ_RS02985
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sulfate 125 mM remove 554,009 - -1.8 554,009 - -1.8 554,010 + +1.5 554,010 + +0.4 554,010 + -1.6 554,011 - -1.5 554,023 - +1.9 554,024 + -3.9 554,095 - -1.2 554,095 - -1.2 554,095 - -0.5 554,096 + -0.1 554,096 + -1.0 554,097 - -0.9 554,098 + +0.4 554,116 + +1.1 554,120 - +2.0 554,121 + -1.8 554,121 + -3.7 554,121 + -0.8 554,121 + -1.2 554,122 - +0.2 554,123 + +2.1 554,147 + +1.4 554,147 + +0.6 554,160 + +3.1 554,181 + +1.8 554,242 - MMJJ_RS02970 0.32 +1.3 554,246 - MMJJ_RS02970 0.33 -1.6 554,247 + MMJJ_RS02970 0.34 +0.9 554,247 + MMJJ_RS02970 0.34 -3.8 554,260 - MMJJ_RS02970 0.39 +0.4 554,273 - MMJJ_RS02970 0.44 +0.7 554,273 - MMJJ_RS02970 0.44 +0.2 554,274 + MMJJ_RS02970 0.45 +2.6 554,297 - MMJJ_RS02970 0.54 -1.6 554,297 - MMJJ_RS02970 0.54 -2.3 554,297 - MMJJ_RS02970 0.54 +0.4 554,297 - MMJJ_RS02970 0.54 +1.1 554,297 - MMJJ_RS02970 0.54 -1.9 554,298 + MMJJ_RS02970 0.54 +0.2 554,298 + MMJJ_RS02970 0.54 +2.4 554,298 + MMJJ_RS02970 0.54 -3.8 554,300 - MMJJ_RS02970 0.55 -0.7 554,302 - MMJJ_RS02970 0.56 +2.6 554,302 - MMJJ_RS02970 0.56 +0.4 554,303 + MMJJ_RS02970 0.56 +0.3 554,303 + MMJJ_RS02970 0.56 -3.5 554,303 + MMJJ_RS02970 0.56 -1.3 554,303 + MMJJ_RS02970 0.56 +0.8 554,303 + MMJJ_RS02970 0.56 +1.6 554,318 + MMJJ_RS02970 0.62 -0.3 554,321 + MMJJ_RS02970 0.63 +0.4 554,353 - MMJJ_RS02970 0.76 -0.8 554,353 - MMJJ_RS02970 0.76 +0.1 554,354 + MMJJ_RS02970 0.77 +0.9 554,354 + MMJJ_RS02970 0.77 -0.3 554,354 + MMJJ_RS02970 0.77 +0.3 554,355 - MMJJ_RS02970 0.77 -3.0 554,356 + MMJJ_RS02970 0.78 +0.5 554,357 - MMJJ_RS02970 0.78 -0.5 554,357 - MMJJ_RS02970 0.78 +1.4 554,357 - MMJJ_RS02970 0.78 -3.3 554,358 + MMJJ_RS02970 0.78 +1.1 554,358 + MMJJ_RS02970 0.78 -2.6 554,372 + MMJJ_RS02970 0.84 -0.8 554,380 - MMJJ_RS02970 0.87 +0.3 554,381 + MMJJ_RS02970 0.88 -3.0 554,382 - MMJJ_RS02970 0.88 -0.7 554,382 - MMJJ_RS02970 0.88 -0.0 554,382 - MMJJ_RS02970 0.88 -1.5 554,382 - MMJJ_RS02970 0.88 -2.6 554,382 - MMJJ_RS02970 0.88 -1.6 554,382 - MMJJ_RS02970 0.88 +1.8 554,382 - MMJJ_RS02970 0.88 -0.0 554,383 + MMJJ_RS02970 0.88 -1.3 554,383 + MMJJ_RS02970 0.88 +1.6 554,383 + MMJJ_RS02970 0.88 +1.0 554,383 + MMJJ_RS02970 0.88 -0.2 554,383 + MMJJ_RS02970 0.88 -2.8 554,383 + MMJJ_RS02970 0.88 -3.8 554,383 + MMJJ_RS02970 0.88 -0.6 554,383 + MMJJ_RS02970 0.88 +0.9 554,392 + +0.5 554,392 + +1.3 554,392 + +2.3 554,395 + -1.4 554,427 + -0.4 554,469 - +2.5 554,476 - +0.7 554,477 + -1.2 554,477 + +0.2 554,477 + -1.0 554,477 + +3.3 554,477 + +0.3 554,480 - -3.1 554,496 - -0.6 554,497 + -1.7 554,522 - MMJJ_RS02975 0.15 +1.0 554,522 - MMJJ_RS02975 0.15 +3.2 554,531 - MMJJ_RS02975 0.16 -0.8 554,532 + MMJJ_RS02975 0.17 +2.6 554,532 + MMJJ_RS02975 0.17 +3.2 554,551 - MMJJ_RS02975 0.20 -4.0 554,594 + MMJJ_RS02975 0.29 -0.5 554,594 + MMJJ_RS02975 0.29 +1.1 554,653 + MMJJ_RS02975 0.41 -1.0 554,713 - MMJJ_RS02975 0.53 +2.0 554,718 + MMJJ_RS02975 0.54 +1.2 554,888 - MMJJ_RS02975 0.88 -1.9 554,888 - MMJJ_RS02975 0.88 -1.1 554,889 + MMJJ_RS02975 0.89 -0.7 554,892 - MMJJ_RS02975 0.89 -1.9 554,892 - MMJJ_RS02975 0.89 -1.2 554,892 - MMJJ_RS02975 0.89 -1.5 554,892 - MMJJ_RS02975 0.89 +0.1 554,893 + MMJJ_RS02975 0.89 -0.7 554,919 - -0.2 554,919 - -2.4 554,919 - -2.2 554,919 - -1.5 554,919 - +1.3 554,919 - -3.1 554,919 - +1.0 554,919 - -4.0 554,919 - -3.1 554,920 + +3.4 554,920 + -0.6 554,920 + -2.8 554,920 + -3.3 554,922 + +0.1 554,944 - -0.6 554,945 + -1.0 554,945 + +1.6 554,946 - +0.1 554,946 - -3.7 554,946 - -2.3 554,946 - +1.7 554,947 + +3.6 554,947 + +0.7 554,960 - +1.1 554,960 - -0.2 554,965 + -2.9 554,965 + -1.5 554,968 - +1.4 554,968 - -2.9 554,975 + +1.1 554,992 - +0.6 554,992 - +0.1 554,992 - -4.1 554,995 - -1.5 554,995 - -0.5 555,011 - +0.9 555,011 - -0.9 555,012 + +1.1 555,012 + +0.2 555,041 - MMJJ_RS02980 0.10 -1.2 555,071 - MMJJ_RS02980 0.14 -0.5 555,071 - MMJJ_RS02980 0.14 +0.8 555,071 - MMJJ_RS02980 0.14 -2.6 555,071 - MMJJ_RS02980 0.14 +1.1 555,072 + MMJJ_RS02980 0.14 +0.9 555,072 + MMJJ_RS02980 0.14 -2.0 555,072 + MMJJ_RS02980 0.14 +1.3 555,072 + MMJJ_RS02980 0.14 +0.7 555,072 + MMJJ_RS02980 0.14 +4.5 555,094 - MMJJ_RS02980 0.17 -0.1 555,100 - MMJJ_RS02980 0.18 +1.8 555,105 + MMJJ_RS02980 0.19 +2.8 555,138 + MMJJ_RS02980 0.23 +1.6 555,182 + MMJJ_RS02980 0.29 -3.7 555,213 + MMJJ_RS02980 0.33 -1.3 555,243 + MMJJ_RS02980 0.37 -0.3 555,243 + MMJJ_RS02980 0.37 -2.3 555,326 + MMJJ_RS02980 0.48 +1.8 555,344 - MMJJ_RS02980 0.50 +1.0 555,344 - MMJJ_RS02980 0.50 -2.7 555,378 + MMJJ_RS02980 0.54 -2.4 555,465 - MMJJ_RS02980 0.66 +0.7 555,523 - MMJJ_RS02980 0.73 -3.5 555,523 - MMJJ_RS02980 0.73 +2.2 555,524 + MMJJ_RS02980 0.74 +1.4 555,647 + MMJJ_RS02980 0.90 -4.1 555,760 - +0.7 555,761 + -1.0 555,791 + -3.4 555,794 - +2.4 555,803 + +2.7 555,812 + +0.5 555,844 - +0.5 555,844 - +1.6 555,847 + +0.5 555,847 + -0.8 555,847 + +1.3 555,849 + -2.0 555,855 - +0.8 555,855 - +1.3 555,856 + +0.9 555,863 + +2.2 555,863 + +1.6 555,872 - +1.1 555,872 - +1.1 555,872 - +2.7 555,873 + +3.0 555,944 - -0.7 555,975 + -0.3 556,029 - MMJJ_RS02985 0.10 +2.1 556,110 - MMJJ_RS02985 0.17 +1.9 556,110 - MMJJ_RS02985 0.17 +2.8 556,111 + MMJJ_RS02985 0.17 +1.4 556,126 - MMJJ_RS02985 0.18 -0.7 556,126 - MMJJ_RS02985 0.18 +2.7 556,127 + MMJJ_RS02985 0.18 +0.1 556,233 - MMJJ_RS02985 0.26 +1.4 556,261 + MMJJ_RS02985 0.28 +0.9 556,264 - MMJJ_RS02985 0.28 +2.1 556,347 + MMJJ_RS02985 0.35 +3.9 556,347 + MMJJ_RS02985 0.35 -0.4 556,373 + MMJJ_RS02985 0.37 +1.6 556,409 - MMJJ_RS02985 0.39 +1.2 556,471 - MMJJ_RS02985 0.44 +1.3 556,471 - MMJJ_RS02985 0.44 +2.3 556,471 - MMJJ_RS02985 0.44 +1.1 556,472 + MMJJ_RS02985 0.44 +3.2 556,472 + MMJJ_RS02985 0.44 -0.3 556,472 + MMJJ_RS02985 0.44 -2.6 556,472 + MMJJ_RS02985 0.44 +4.2 556,472 + MMJJ_RS02985 0.44 +2.2 556,472 + MMJJ_RS02985 0.44 -3.4 556,472 + MMJJ_RS02985 0.44 +1.8 556,495 + MMJJ_RS02985 0.46 -1.8 556,562 - MMJJ_RS02985 0.51 +0.6 556,563 + MMJJ_RS02985 0.51 +1.0 556,607 - MMJJ_RS02985 0.55 -2.1 556,610 - MMJJ_RS02985 0.55 -2.4 556,611 + MMJJ_RS02985 0.55 +3.1 556,673 - MMJJ_RS02985 0.60 +5.1 556,673 - MMJJ_RS02985 0.60 -3.0
Or see this region's nucleotide sequence