Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08535 and MMJJ_RS08540 are separated by 52 nucleotides MMJJ_RS08540 and MMJJ_RS08545 are separated by 42 nucleotides MMJJ_RS08545 and MMJJ_RS08550 are separated by 342 nucleotides
MMJJ_RS08535: MMJJ_RS08535 - NOL1/NOP2/sun family putative RNA methylase, at 1,570,900 to 1,571,697
_RS08535
MMJJ_RS08540: MMJJ_RS08540 - archaeosortase family protein ArtE, at 1,571,750 to 1,572,223
_RS08540
MMJJ_RS08545: MMJJ_RS08545 - thiamine-phosphate kinase, at 1,572,266 to 1,573,213
_RS08545
MMJJ_RS08550: MMJJ_RS08550 - B12-binding domain-containing radical SAM protein, at 1,573,556 to 1,574,872
_RS08550
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1571.266 kb on - strand, within MMJJ_RS08535 at 1571.589 kb on + strand, within MMJJ_RS08535 at 1571.714 kb on - strand at 1571.718 kb on + strand at 1571.720 kb on - strand at 1571.722 kb on - strand at 1571.726 kb on + strand at 1571.726 kb on + strand at 1571.729 kb on - strand at 1571.730 kb on + strand at 1571.730 kb on + strand at 1571.732 kb on - strand at 1571.754 kb on + strand at 1571.858 kb on + strand, within MMJJ_RS08540 at 1571.887 kb on - strand, within MMJJ_RS08540 at 1571.887 kb on - strand, within MMJJ_RS08540 at 1571.888 kb on + strand, within MMJJ_RS08540 at 1571.888 kb on + strand, within MMJJ_RS08540 at 1571.891 kb on - strand, within MMJJ_RS08540 at 1571.891 kb on - strand, within MMJJ_RS08540 at 1571.891 kb on - strand, within MMJJ_RS08540 at 1571.892 kb on + strand, within MMJJ_RS08540 at 1571.892 kb on + strand, within MMJJ_RS08540 at 1571.892 kb on + strand, within MMJJ_RS08540 at 1571.904 kb on - strand, within MMJJ_RS08540 at 1571.904 kb on - strand, within MMJJ_RS08540 at 1571.905 kb on + strand, within MMJJ_RS08540 at 1571.905 kb on + strand, within MMJJ_RS08540 at 1571.905 kb on + strand, within MMJJ_RS08540 at 1571.905 kb on + strand, within MMJJ_RS08540 at 1571.906 kb on - strand, within MMJJ_RS08540 at 1571.907 kb on + strand, within MMJJ_RS08540 at 1571.907 kb on + strand, within MMJJ_RS08540 at 1571.910 kb on + strand, within MMJJ_RS08540 at 1571.917 kb on - strand, within MMJJ_RS08540 at 1571.918 kb on + strand, within MMJJ_RS08540 at 1571.918 kb on + strand, within MMJJ_RS08540 at 1571.918 kb on + strand, within MMJJ_RS08540 at 1571.924 kb on - strand, within MMJJ_RS08540 at 1571.941 kb on + strand, within MMJJ_RS08540 at 1571.941 kb on + strand, within MMJJ_RS08540 at 1571.941 kb on + strand, within MMJJ_RS08540 at 1571.944 kb on + strand, within MMJJ_RS08540 at 1571.949 kb on + strand, within MMJJ_RS08540 at 1571.969 kb on - strand, within MMJJ_RS08540 at 1571.969 kb on - strand, within MMJJ_RS08540 at 1571.969 kb on - strand, within MMJJ_RS08540 at 1571.969 kb on - strand, within MMJJ_RS08540 at 1571.969 kb on - strand, within MMJJ_RS08540 at 1571.970 kb on + strand, within MMJJ_RS08540 at 1571.972 kb on + strand, within MMJJ_RS08540 at 1571.982 kb on + strand, within MMJJ_RS08540 at 1571.982 kb on + strand, within MMJJ_RS08540 at 1572.007 kb on - strand, within MMJJ_RS08540 at 1572.008 kb on + strand, within MMJJ_RS08540 at 1572.029 kb on - strand, within MMJJ_RS08540 at 1572.029 kb on - strand, within MMJJ_RS08540 at 1572.047 kb on - strand, within MMJJ_RS08540 at 1572.048 kb on + strand, within MMJJ_RS08540 at 1572.048 kb on + strand, within MMJJ_RS08540 at 1572.108 kb on - strand, within MMJJ_RS08540 at 1572.146 kb on - strand, within MMJJ_RS08540 at 1572.147 kb on + strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.149 kb on - strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.150 kb on + strand, within MMJJ_RS08540 at 1572.151 kb on - strand, within MMJJ_RS08540 at 1572.151 kb on - strand, within MMJJ_RS08540 at 1572.151 kb on - strand, within MMJJ_RS08540 at 1572.151 kb on - strand, within MMJJ_RS08540 at 1572.151 kb on - strand, within MMJJ_RS08540 at 1572.151 kb on - strand, within MMJJ_RS08540 at 1572.152 kb on + strand, within MMJJ_RS08540 at 1572.152 kb on + strand, within MMJJ_RS08540 at 1572.152 kb on + strand, within MMJJ_RS08540 at 1572.163 kb on - strand, within MMJJ_RS08540 at 1572.164 kb on + strand, within MMJJ_RS08540 at 1572.164 kb on + strand, within MMJJ_RS08540 at 1572.165 kb on - strand, within MMJJ_RS08540 at 1572.165 kb on - strand, within MMJJ_RS08540 at 1572.166 kb on + strand, within MMJJ_RS08540 at 1572.166 kb on + strand, within MMJJ_RS08540 at 1572.171 kb on - strand, within MMJJ_RS08540 at 1572.171 kb on - strand, within MMJJ_RS08540 at 1572.171 kb on - strand, within MMJJ_RS08540 at 1572.171 kb on - strand, within MMJJ_RS08540 at 1572.172 kb on + strand, within MMJJ_RS08540 at 1572.186 kb on + strand at 1572.202 kb on + strand at 1572.228 kb on - strand at 1572.229 kb on + strand at 1572.248 kb on - strand at 1572.248 kb on - strand at 1572.248 kb on - strand at 1572.248 kb on - strand at 1572.248 kb on - strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.249 kb on + strand at 1572.250 kb on - strand at 1572.250 kb on - strand at 1572.255 kb on - strand at 1572.255 kb on - strand at 1572.256 kb on + strand at 1572.256 kb on + strand at 1572.256 kb on + strand at 1572.256 kb on + strand at 1572.259 kb on - strand at 1572.259 kb on - strand at 1572.259 kb on - strand at 1572.260 kb on + strand at 1572.260 kb on + strand at 1572.262 kb on - strand at 1572.268 kb on + strand at 1572.282 kb on - strand at 1572.283 kb on + strand at 1572.540 kb on - strand, within MMJJ_RS08545 at 1572.795 kb on - strand at 1572.797 kb on - strand, within MMJJ_RS08545 at 1572.811 kb on - strand, within MMJJ_RS08545 at 1573.195 kb on - strand at 1573.195 kb on - strand at 1573.271 kb on - strand at 1573.276 kb on + strand at 1573.278 kb on - strand at 1573.278 kb on - strand at 1573.279 kb on + strand at 1573.279 kb on + strand at 1573.279 kb on + strand at 1573.279 kb on + strand at 1573.282 kb on - strand at 1573.282 kb on - strand at 1573.282 kb on - strand at 1573.283 kb on + strand at 1573.288 kb on - strand at 1573.288 kb on - strand at 1573.302 kb on + strand at 1573.311 kb on - strand at 1573.320 kb on - strand at 1573.345 kb on - strand at 1573.345 kb on - strand at 1573.346 kb on + strand at 1573.347 kb on - strand at 1573.350 kb on + strand at 1573.363 kb on - strand at 1573.366 kb on - strand at 1573.367 kb on + strand at 1573.432 kb on - strand at 1573.433 kb on + strand at 1573.445 kb on + strand at 1573.445 kb on + strand at 1573.480 kb on - strand at 1573.501 kb on - strand at 1573.501 kb on - strand at 1573.501 kb on - strand at 1573.502 kb on + strand at 1573.504 kb on + strand at 1573.515 kb on - strand at 1573.515 kb on - strand at 1573.516 kb on + strand at 1573.519 kb on - strand at 1573.529 kb on - strand at 1573.529 kb on - strand at 1573.529 kb on - strand at 1573.531 kb on - strand at 1573.531 kb on - strand at 1573.534 kb on - strand at 1573.534 kb on - strand at 1573.535 kb on + strand at 1573.644 kb on - strand at 1573.652 kb on - strand at 1573.652 kb on - strand at 1573.676 kb on - strand at 1573.682 kb on + strand at 1573.722 kb on - strand, within MMJJ_RS08550 at 1573.808 kb on + strand, within MMJJ_RS08550 at 1573.941 kb on - strand, within MMJJ_RS08550 at 1573.941 kb on - strand, within MMJJ_RS08550 at 1573.942 kb on + strand, within MMJJ_RS08550 at 1573.944 kb on + strand, within MMJJ_RS08550 at 1573.944 kb on + strand, within MMJJ_RS08550 at 1573.960 kb on - strand, within MMJJ_RS08550 at 1573.984 kb on + strand, within MMJJ_RS08550 at 1573.984 kb on + strand, within MMJJ_RS08550 at 1574.030 kb on + strand, within MMJJ_RS08550 at 1574.049 kb on + strand, within MMJJ_RS08550 at 1574.061 kb on + strand, within MMJJ_RS08550 at 1574.061 kb on + strand, within MMJJ_RS08550 at 1574.123 kb on - strand, within MMJJ_RS08550 at 1574.123 kb on - strand, within MMJJ_RS08550 at 1574.124 kb on + strand, within MMJJ_RS08550 at 1574.124 kb on + strand, within MMJJ_RS08550 at 1574.158 kb on + strand, within MMJJ_RS08550 at 1574.162 kb on - strand, within MMJJ_RS08550 at 1574.211 kb on - strand, within MMJJ_RS08550
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM remove 1,571,266 - MMJJ_RS08535 0.46 -1.6 1,571,589 + MMJJ_RS08535 0.86 -3.0 1,571,714 - -0.2 1,571,718 + -0.0 1,571,720 - +0.3 1,571,722 - -0.1 1,571,726 + -0.4 1,571,726 + -0.8 1,571,729 - -2.9 1,571,730 + -2.6 1,571,730 + -0.9 1,571,732 - +2.1 1,571,754 + -1.9 1,571,858 + MMJJ_RS08540 0.23 +0.2 1,571,887 - MMJJ_RS08540 0.29 +0.4 1,571,887 - MMJJ_RS08540 0.29 +3.2 1,571,888 + MMJJ_RS08540 0.29 +1.1 1,571,888 + MMJJ_RS08540 0.29 +1.3 1,571,891 - MMJJ_RS08540 0.30 -0.0 1,571,891 - MMJJ_RS08540 0.30 -3.3 1,571,891 - MMJJ_RS08540 0.30 +0.7 1,571,892 + MMJJ_RS08540 0.30 -3.5 1,571,892 + MMJJ_RS08540 0.30 +4.5 1,571,892 + MMJJ_RS08540 0.30 -2.8 1,571,904 - MMJJ_RS08540 0.32 -2.3 1,571,904 - MMJJ_RS08540 0.32 +1.2 1,571,905 + MMJJ_RS08540 0.33 -2.1 1,571,905 + MMJJ_RS08540 0.33 -0.5 1,571,905 + MMJJ_RS08540 0.33 +0.6 1,571,905 + MMJJ_RS08540 0.33 +1.7 1,571,906 - MMJJ_RS08540 0.33 +0.2 1,571,907 + MMJJ_RS08540 0.33 +1.3 1,571,907 + MMJJ_RS08540 0.33 +1.9 1,571,910 + MMJJ_RS08540 0.34 -0.2 1,571,917 - MMJJ_RS08540 0.35 -1.5 1,571,918 + MMJJ_RS08540 0.35 -1.0 1,571,918 + MMJJ_RS08540 0.35 -3.3 1,571,918 + MMJJ_RS08540 0.35 +0.8 1,571,924 - MMJJ_RS08540 0.37 -1.0 1,571,941 + MMJJ_RS08540 0.40 -1.6 1,571,941 + MMJJ_RS08540 0.40 -0.1 1,571,941 + MMJJ_RS08540 0.40 +1.3 1,571,944 + MMJJ_RS08540 0.41 +0.8 1,571,949 + MMJJ_RS08540 0.42 +2.1 1,571,969 - MMJJ_RS08540 0.46 +0.0 1,571,969 - MMJJ_RS08540 0.46 +2.7 1,571,969 - MMJJ_RS08540 0.46 -2.6 1,571,969 - MMJJ_RS08540 0.46 +1.5 1,571,969 - MMJJ_RS08540 0.46 +1.0 1,571,970 + MMJJ_RS08540 0.46 +1.5 1,571,972 + MMJJ_RS08540 0.47 -0.4 1,571,982 + MMJJ_RS08540 0.49 +1.8 1,571,982 + MMJJ_RS08540 0.49 -0.8 1,572,007 - MMJJ_RS08540 0.54 -0.9 1,572,008 + MMJJ_RS08540 0.54 +1.6 1,572,029 - MMJJ_RS08540 0.59 -1.6 1,572,029 - MMJJ_RS08540 0.59 -0.9 1,572,047 - MMJJ_RS08540 0.63 -1.1 1,572,048 + MMJJ_RS08540 0.63 +4.3 1,572,048 + MMJJ_RS08540 0.63 +2.0 1,572,108 - MMJJ_RS08540 0.76 -1.1 1,572,146 - MMJJ_RS08540 0.84 +0.2 1,572,147 + MMJJ_RS08540 0.84 -1.6 1,572,149 - MMJJ_RS08540 0.84 -2.2 1,572,149 - MMJJ_RS08540 0.84 +2.0 1,572,149 - MMJJ_RS08540 0.84 -1.4 1,572,149 - MMJJ_RS08540 0.84 -0.0 1,572,149 - MMJJ_RS08540 0.84 +1.2 1,572,149 - MMJJ_RS08540 0.84 +2.8 1,572,149 - MMJJ_RS08540 0.84 +1.1 1,572,149 - MMJJ_RS08540 0.84 +1.1 1,572,149 - MMJJ_RS08540 0.84 +1.4 1,572,149 - +2.6 1,572,149 - MMJJ_RS08540 0.84 -1.2 1,572,149 - MMJJ_RS08540 0.84 +1.4 1,572,149 - MMJJ_RS08540 0.84 -2.3 1,572,149 - MMJJ_RS08540 0.84 +1.8 1,572,150 + MMJJ_RS08540 0.84 +3.8 1,572,150 + MMJJ_RS08540 0.84 +1.1 1,572,150 + MMJJ_RS08540 0.84 -2.7 1,572,150 + MMJJ_RS08540 0.84 -2.8 1,572,150 + MMJJ_RS08540 0.84 +0.3 1,572,150 + MMJJ_RS08540 0.84 +0.5 1,572,150 + MMJJ_RS08540 0.84 +0.7 1,572,150 + MMJJ_RS08540 0.84 -3.6 1,572,150 + MMJJ_RS08540 0.84 -0.7 1,572,150 + MMJJ_RS08540 0.84 +0.2 1,572,151 - MMJJ_RS08540 0.85 -0.8 1,572,151 - MMJJ_RS08540 0.85 -2.1 1,572,151 - MMJJ_RS08540 0.85 +2.4 1,572,151 - MMJJ_RS08540 0.85 +1.3 1,572,151 - MMJJ_RS08540 0.85 -3.8 1,572,151 - MMJJ_RS08540 0.85 +0.4 1,572,152 + MMJJ_RS08540 0.85 -1.7 1,572,152 + MMJJ_RS08540 0.85 -2.0 1,572,152 + MMJJ_RS08540 0.85 +3.3 1,572,163 - MMJJ_RS08540 0.87 -0.1 1,572,164 + MMJJ_RS08540 0.87 +0.9 1,572,164 + MMJJ_RS08540 0.87 -3.9 1,572,165 - MMJJ_RS08540 0.88 +0.4 1,572,165 - MMJJ_RS08540 0.88 -0.9 1,572,166 + MMJJ_RS08540 0.88 +1.7 1,572,166 + MMJJ_RS08540 0.88 +1.0 1,572,171 - MMJJ_RS08540 0.89 +1.5 1,572,171 - MMJJ_RS08540 0.89 +1.0 1,572,171 - MMJJ_RS08540 0.89 -1.5 1,572,171 - MMJJ_RS08540 0.89 +1.6 1,572,172 + MMJJ_RS08540 0.89 -3.5 1,572,186 + -0.4 1,572,202 + +0.2 1,572,228 - +4.0 1,572,229 + -0.1 1,572,248 - +2.3 1,572,248 - -3.8 1,572,248 - -0.4 1,572,248 - +2.6 1,572,248 - -2.4 1,572,249 + -2.6 1,572,249 + +0.2 1,572,249 + +1.6 1,572,249 + -2.6 1,572,249 + -0.8 1,572,249 + -2.3 1,572,249 + +2.4 1,572,249 + -1.2 1,572,250 - -1.9 1,572,250 - -3.5 1,572,255 - +1.0 1,572,255 - -1.6 1,572,256 + +5.5 1,572,256 + -0.1 1,572,256 + -2.1 1,572,256 + +2.4 1,572,259 - -0.2 1,572,259 - -2.0 1,572,259 - +2.5 1,572,260 + -1.8 1,572,260 + -2.3 1,572,262 - -1.3 1,572,268 + -0.9 1,572,282 - -0.6 1,572,283 + +0.6 1,572,540 - MMJJ_RS08545 0.29 +2.0 1,572,795 - +1.0 1,572,797 - MMJJ_RS08545 0.56 +0.4 1,572,811 - MMJJ_RS08545 0.57 -1.4 1,573,195 - +1.0 1,573,195 - +2.0 1,573,271 - +0.6 1,573,276 + +1.4 1,573,278 - -2.0 1,573,278 - +1.3 1,573,279 + -1.6 1,573,279 + -1.8 1,573,279 + +2.2 1,573,279 + +4.0 1,573,282 - -1.4 1,573,282 - -4.4 1,573,282 - +0.6 1,573,283 + -3.4 1,573,288 - +3.3 1,573,288 - -2.3 1,573,302 + -1.9 1,573,311 - +2.6 1,573,320 - +1.0 1,573,345 - +0.8 1,573,345 - +1.1 1,573,346 + +2.4 1,573,347 - +3.3 1,573,350 + +2.3 1,573,363 - +0.9 1,573,366 - -0.7 1,573,367 + +1.0 1,573,432 - -0.8 1,573,433 + -0.7 1,573,445 + -3.2 1,573,445 + -1.9 1,573,480 - -2.4 1,573,501 - -0.6 1,573,501 - -1.6 1,573,501 - +1.0 1,573,502 + -1.4 1,573,504 + +2.9 1,573,515 - -2.0 1,573,515 - -3.5 1,573,516 + +1.9 1,573,519 - -0.1 1,573,529 - -1.4 1,573,529 - +1.0 1,573,529 - +1.6 1,573,531 - -0.2 1,573,531 - +0.9 1,573,534 - +0.5 1,573,534 - -1.6 1,573,535 + +1.4 1,573,644 - -0.6 1,573,652 - -1.2 1,573,652 - -2.1 1,573,676 - +0.7 1,573,682 + +0.6 1,573,722 - MMJJ_RS08550 0.13 -1.2 1,573,808 + MMJJ_RS08550 0.19 +2.4 1,573,941 - MMJJ_RS08550 0.29 -0.3 1,573,941 - MMJJ_RS08550 0.29 -1.9 1,573,942 + MMJJ_RS08550 0.29 +0.1 1,573,944 + MMJJ_RS08550 0.29 +0.5 1,573,944 + MMJJ_RS08550 0.29 +0.4 1,573,960 - MMJJ_RS08550 0.31 -0.2 1,573,984 + MMJJ_RS08550 0.32 +0.4 1,573,984 + MMJJ_RS08550 0.32 +1.5 1,574,030 + MMJJ_RS08550 0.36 -1.1 1,574,049 + MMJJ_RS08550 0.37 +1.1 1,574,061 + MMJJ_RS08550 0.38 -2.6 1,574,061 + MMJJ_RS08550 0.38 -0.4 1,574,123 - MMJJ_RS08550 0.43 -1.8 1,574,123 - MMJJ_RS08550 0.43 +1.2 1,574,124 + MMJJ_RS08550 0.43 -2.9 1,574,124 + MMJJ_RS08550 0.43 -0.8 1,574,158 + MMJJ_RS08550 0.46 -3.4 1,574,162 - MMJJ_RS08550 0.46 +0.5 1,574,211 - MMJJ_RS08550 0.50 +1.8
Or see this region's nucleotide sequence