Experiment: McCas_formate_complete sodium nitrite 0.416666666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03700 and MMJJ_RS03705 are separated by 8 nucleotides MMJJ_RS03705 and MMJJ_RS03710 are separated by 20 nucleotides MMJJ_RS03710 and MMJJ_RS03715 are separated by 54 nucleotides MMJJ_RS03715 and MMJJ_RS03720 are separated by 123 nucleotides MMJJ_RS03720 and MMJJ_RS03725 are separated by 9 nucleotides MMJJ_RS03725 and MMJJ_RS03730 are separated by 156 nucleotides
MMJJ_RS03700: MMJJ_RS03700 - hypothetical protein, at 693,021 to 693,476
_RS03700
MMJJ_RS03705: MMJJ_RS03705 - methanogenesis marker 5 protein, at 693,485 to 693,925
_RS03705
MMJJ_RS03710: MMJJ_RS03710 - DUF2111 domain-containing protein, at 693,946 to 694,329
_RS03710
MMJJ_RS03715: MMJJ_RS03715 - hypothetical protein, at 694,384 to 694,968
_RS03715
MMJJ_RS03720: MMJJ_RS03720 - DsrE family protein, at 695,092 to 695,436
_RS03720
MMJJ_RS03725: MMJJ_RS03725 - sulfurtransferase TusA family protein, at 695,446 to 695,658
_RS03725
MMJJ_RS03730: MMJJ_RS03730 - SWIM zinc finger family protein, at 695,815 to 696,504
_RS03730
Position (kb) 694
695 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 693.409 kb on + strand, within MMJJ_RS03700 at 693.458 kb on - strand at 693.782 kb on - strand, within MMJJ_RS03705 at 693.784 kb on - strand at 693.928 kb on - strand at 693.947 kb on - strand at 693.948 kb on + strand at 693.948 kb on + strand at 693.948 kb on + strand at 694.034 kb on + strand, within MMJJ_RS03710 at 694.040 kb on + strand, within MMJJ_RS03710 at 694.040 kb on + strand, within MMJJ_RS03710 at 694.044 kb on - strand, within MMJJ_RS03710 at 694.044 kb on - strand, within MMJJ_RS03710 at 694.044 kb on - strand, within MMJJ_RS03710 at 694.044 kb on - strand, within MMJJ_RS03710 at 694.048 kb on - strand, within MMJJ_RS03710 at 694.049 kb on + strand, within MMJJ_RS03710 at 694.049 kb on + strand, within MMJJ_RS03710 at 694.078 kb on - strand, within MMJJ_RS03710 at 694.079 kb on + strand, within MMJJ_RS03710 at 694.122 kb on - strand, within MMJJ_RS03710 at 694.165 kb on - strand, within MMJJ_RS03710 at 694.173 kb on + strand, within MMJJ_RS03710 at 694.195 kb on - strand, within MMJJ_RS03710 at 694.216 kb on + strand, within MMJJ_RS03710 at 694.267 kb on - strand, within MMJJ_RS03710 at 694.268 kb on + strand, within MMJJ_RS03710 at 694.273 kb on - strand, within MMJJ_RS03710 at 694.274 kb on + strand, within MMJJ_RS03710 at 694.274 kb on + strand, within MMJJ_RS03710 at 694.275 kb on - strand, within MMJJ_RS03710 at 694.294 kb on - strand at 694.295 kb on + strand at 694.302 kb on - strand at 694.303 kb on + strand at 694.342 kb on - strand at 694.342 kb on - strand at 694.346 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.354 kb on - strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.355 kb on + strand at 694.358 kb on - strand at 694.358 kb on - strand at 694.359 kb on + strand at 694.368 kb on - strand at 694.369 kb on + strand at 694.369 kb on + strand at 694.375 kb on + strand at 694.407 kb on - strand at 694.408 kb on + strand at 694.559 kb on - strand, within MMJJ_RS03715 at 694.559 kb on - strand, within MMJJ_RS03715 at 694.567 kb on + strand, within MMJJ_RS03715 at 694.610 kb on - strand, within MMJJ_RS03715 at 694.621 kb on - strand, within MMJJ_RS03715 at 694.622 kb on + strand, within MMJJ_RS03715 at 694.663 kb on - strand, within MMJJ_RS03715 at 694.679 kb on + strand, within MMJJ_RS03715 at 694.743 kb on + strand, within MMJJ_RS03715 at 694.784 kb on + strand, within MMJJ_RS03715 at 694.798 kb on + strand, within MMJJ_RS03715 at 694.816 kb on - strand, within MMJJ_RS03715 at 694.817 kb on + strand at 694.839 kb on - strand, within MMJJ_RS03715 at 694.850 kb on - strand, within MMJJ_RS03715 at 694.850 kb on - strand, within MMJJ_RS03715 at 694.850 kb on - strand, within MMJJ_RS03715 at 694.851 kb on + strand, within MMJJ_RS03715 at 694.851 kb on + strand at 694.851 kb on + strand, within MMJJ_RS03715 at 694.860 kb on - strand, within MMJJ_RS03715 at 694.870 kb on - strand, within MMJJ_RS03715 at 694.875 kb on - strand, within MMJJ_RS03715 at 694.875 kb on - strand, within MMJJ_RS03715 at 694.876 kb on + strand, within MMJJ_RS03715 at 694.886 kb on - strand, within MMJJ_RS03715 at 694.887 kb on + strand, within MMJJ_RS03715 at 694.890 kb on - strand, within MMJJ_RS03715 at 694.891 kb on + strand, within MMJJ_RS03715 at 694.940 kb on + strand at 694.963 kb on + strand at 694.968 kb on - strand at 694.969 kb on + strand at 694.969 kb on + strand at 694.984 kb on - strand at 694.985 kb on + strand at 694.990 kb on + strand at 694.994 kb on - strand at 695.013 kb on - strand at 695.027 kb on - strand at 695.033 kb on - strand at 695.033 kb on - strand at 695.033 kb on - strand at 695.059 kb on - strand at 695.060 kb on + strand at 695.101 kb on + strand at 695.122 kb on - strand at 695.123 kb on + strand at 695.131 kb on - strand, within MMJJ_RS03720 at 695.132 kb on + strand, within MMJJ_RS03720 at 695.134 kb on - strand, within MMJJ_RS03720 at 695.135 kb on + strand, within MMJJ_RS03720 at 695.221 kb on - strand, within MMJJ_RS03720 at 695.222 kb on + strand, within MMJJ_RS03720 at 695.264 kb on - strand, within MMJJ_RS03720 at 695.265 kb on + strand, within MMJJ_RS03720 at 695.306 kb on - strand, within MMJJ_RS03720 at 695.307 kb on + strand, within MMJJ_RS03720 at 695.307 kb on + strand, within MMJJ_RS03720 at 695.329 kb on - strand, within MMJJ_RS03720 at 695.330 kb on + strand, within MMJJ_RS03720 at 695.330 kb on + strand, within MMJJ_RS03720 at 695.331 kb on - strand, within MMJJ_RS03720 at 695.362 kb on - strand, within MMJJ_RS03720 at 695.362 kb on - strand, within MMJJ_RS03720 at 695.374 kb on - strand, within MMJJ_RS03720 at 695.416 kb on - strand at 695.419 kb on - strand at 695.419 kb on - strand at 695.420 kb on + strand at 695.420 kb on + strand at 695.421 kb on - strand at 695.457 kb on - strand at 695.458 kb on + strand at 695.458 kb on + strand at 695.462 kb on + strand at 695.462 kb on + strand at 695.498 kb on + strand, within MMJJ_RS03725 at 695.511 kb on - strand, within MMJJ_RS03725 at 695.511 kb on - strand, within MMJJ_RS03725 at 695.632 kb on - strand, within MMJJ_RS03725 at 695.681 kb on - strand at 695.687 kb on - strand at 695.732 kb on + strand at 695.747 kb on + strand at 695.755 kb on + strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.763 kb on - strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.764 kb on + strand at 695.768 kb on + strand at 695.771 kb on - strand at 695.784 kb on - strand at 695.837 kb on - strand at 695.838 kb on + strand at 695.838 kb on + strand at 695.844 kb on + strand at 695.863 kb on - strand at 695.864 kb on + strand at 695.864 kb on + strand at 695.883 kb on + strand at 695.917 kb on + strand, within MMJJ_RS03730 at 695.956 kb on - strand, within MMJJ_RS03730 at 695.968 kb on - strand, within MMJJ_RS03730
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium nitrite 0.416666666666667 mM remove 693,409 + MMJJ_RS03700 0.85 -1.1 693,458 - +0.3 693,782 - MMJJ_RS03705 0.67 +1.6 693,784 - +0.6 693,928 - -1.1 693,947 - -1.2 693,948 + +0.3 693,948 + -1.5 693,948 + +0.2 694,034 + MMJJ_RS03710 0.23 +0.6 694,040 + MMJJ_RS03710 0.24 -2.3 694,040 + MMJJ_RS03710 0.24 -1.2 694,044 - MMJJ_RS03710 0.26 -0.9 694,044 - MMJJ_RS03710 0.26 -1.0 694,044 - MMJJ_RS03710 0.26 +1.3 694,044 - MMJJ_RS03710 0.26 +2.5 694,048 - MMJJ_RS03710 0.27 +0.2 694,049 + MMJJ_RS03710 0.27 +0.4 694,049 + MMJJ_RS03710 0.27 -1.0 694,078 - MMJJ_RS03710 0.34 -0.6 694,079 + MMJJ_RS03710 0.35 -1.7 694,122 - MMJJ_RS03710 0.46 -0.8 694,165 - MMJJ_RS03710 0.57 -1.4 694,173 + MMJJ_RS03710 0.59 -0.8 694,195 - MMJJ_RS03710 0.65 -0.7 694,216 + MMJJ_RS03710 0.70 -2.7 694,267 - MMJJ_RS03710 0.84 +0.7 694,268 + MMJJ_RS03710 0.84 +0.3 694,273 - MMJJ_RS03710 0.85 +1.5 694,274 + MMJJ_RS03710 0.85 -2.2 694,274 + MMJJ_RS03710 0.85 -0.4 694,275 - MMJJ_RS03710 0.86 +0.9 694,294 - +2.5 694,295 + +0.6 694,302 - +0.2 694,303 + +0.6 694,342 - +2.4 694,342 - +1.7 694,346 - +1.7 694,354 - -1.7 694,354 - -0.8 694,354 - +1.0 694,354 - -0.3 694,354 - +2.8 694,354 - +1.9 694,354 - +0.7 694,354 - +0.6 694,354 - -3.3 694,354 - +0.2 694,354 - -3.3 694,354 - +0.6 694,354 - +0.8 694,354 - -2.0 694,354 - -2.4 694,355 + -2.8 694,355 + -0.6 694,355 + -0.5 694,355 + -0.8 694,355 + -0.6 694,355 + -2.2 694,355 + -1.4 694,355 + +0.7 694,355 + -1.8 694,355 + -0.8 694,355 + -1.4 694,355 + -1.6 694,355 + +0.0 694,358 - +1.2 694,358 - -0.5 694,359 + -0.2 694,368 - -2.0 694,369 + -1.6 694,369 + -1.2 694,375 + +2.8 694,407 - -1.8 694,408 + -1.3 694,559 - MMJJ_RS03715 0.30 -2.3 694,559 - MMJJ_RS03715 0.30 -0.8 694,567 + MMJJ_RS03715 0.31 +1.2 694,610 - MMJJ_RS03715 0.39 +1.7 694,621 - MMJJ_RS03715 0.41 -2.8 694,622 + MMJJ_RS03715 0.41 +0.0 694,663 - MMJJ_RS03715 0.48 +2.2 694,679 + MMJJ_RS03715 0.50 -0.2 694,743 + MMJJ_RS03715 0.61 +0.8 694,784 + MMJJ_RS03715 0.68 -0.6 694,798 + MMJJ_RS03715 0.71 -0.0 694,816 - MMJJ_RS03715 0.74 +1.5 694,817 + +1.6 694,839 - MMJJ_RS03715 0.78 -1.3 694,850 - MMJJ_RS03715 0.80 -2.1 694,850 - MMJJ_RS03715 0.80 -3.7 694,850 - MMJJ_RS03715 0.80 +0.1 694,851 + MMJJ_RS03715 0.80 -0.7 694,851 + +0.3 694,851 + MMJJ_RS03715 0.80 -2.4 694,860 - MMJJ_RS03715 0.81 -0.3 694,870 - MMJJ_RS03715 0.83 -3.5 694,875 - MMJJ_RS03715 0.84 +0.3 694,875 - MMJJ_RS03715 0.84 +1.1 694,876 + MMJJ_RS03715 0.84 +1.4 694,886 - MMJJ_RS03715 0.86 +2.3 694,887 + MMJJ_RS03715 0.86 +2.7 694,890 - MMJJ_RS03715 0.86 +1.7 694,891 + MMJJ_RS03715 0.87 +3.2 694,940 + +1.1 694,963 + +1.5 694,968 - -0.2 694,969 + +0.7 694,969 + -2.7 694,984 - -1.0 694,985 + +0.7 694,990 + +1.7 694,994 - -1.2 695,013 - +2.9 695,027 - +1.7 695,033 - +2.3 695,033 - -0.7 695,033 - +0.6 695,059 - -0.3 695,060 + +1.0 695,101 + -2.6 695,122 - -1.5 695,123 + +2.3 695,131 - MMJJ_RS03720 0.11 -2.1 695,132 + MMJJ_RS03720 0.12 +1.4 695,134 - MMJJ_RS03720 0.12 +1.6 695,135 + MMJJ_RS03720 0.12 +0.7 695,221 - MMJJ_RS03720 0.37 -1.2 695,222 + MMJJ_RS03720 0.38 -0.6 695,264 - MMJJ_RS03720 0.50 -3.4 695,265 + MMJJ_RS03720 0.50 +2.5 695,306 - MMJJ_RS03720 0.62 +0.5 695,307 + MMJJ_RS03720 0.62 +0.8 695,307 + MMJJ_RS03720 0.62 +1.4 695,329 - MMJJ_RS03720 0.69 -2.6 695,330 + MMJJ_RS03720 0.69 -0.7 695,330 + MMJJ_RS03720 0.69 +0.6 695,331 - MMJJ_RS03720 0.69 -0.8 695,362 - MMJJ_RS03720 0.78 +2.0 695,362 - MMJJ_RS03720 0.78 -2.6 695,374 - MMJJ_RS03720 0.82 +0.8 695,416 - +1.7 695,419 - -1.1 695,419 - +0.2 695,420 + -0.3 695,420 + +0.3 695,421 - +1.7 695,457 - -2.0 695,458 + -2.8 695,458 + +2.3 695,462 + +5.9 695,462 + -4.1 695,498 + MMJJ_RS03725 0.24 +3.4 695,511 - MMJJ_RS03725 0.31 +4.3 695,511 - MMJJ_RS03725 0.31 +2.2 695,632 - MMJJ_RS03725 0.87 +0.3 695,681 - -0.2 695,687 - -0.1 695,732 + +0.1 695,747 + -0.3 695,755 + -3.4 695,763 - +1.0 695,763 - +0.2 695,763 - -1.3 695,763 - +2.4 695,763 - -3.4 695,764 + +1.2 695,764 + -2.3 695,764 + +2.3 695,764 + -1.3 695,768 + -3.5 695,771 - +1.5 695,784 - +2.6 695,837 - +0.8 695,838 + +1.0 695,838 + +0.3 695,844 + +0.6 695,863 - -1.1 695,864 + -2.4 695,864 + -1.9 695,883 + -0.2 695,917 + MMJJ_RS03730 0.15 -2.4 695,956 - MMJJ_RS03730 0.20 -0.5 695,968 - MMJJ_RS03730 0.22 +0.7
Or see this region's nucleotide sequence