Experiment: McCas_formate_complete Rifampicin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05915 and MMJJ_RS05920 are separated by 8 nucleotides MMJJ_RS05920 and MMJJ_RS05925 are separated by 21 nucleotides MMJJ_RS05925 and MMJJ_RS05930 are separated by 23 nucleotides MMJJ_RS05930 and MMJJ_RS05935 are separated by 6 nucleotides
MMJJ_RS05915: MMJJ_RS05915 - multiprotein bridging factor aMBF1, at 1,101,685 to 1,102,161
_RS05915
MMJJ_RS05920: MMJJ_RS05920 - isopentenyl phosphate kinase, at 1,102,170 to 1,102,943
_RS05920
MMJJ_RS05925: MMJJ_RS05925 - DUF2099 family protein, at 1,102,965 to 1,103,816
_RS05925
MMJJ_RS05930: MMJJ_RS05930 - cell division protein SepF, at 1,103,840 to 1,104,229
_RS05930
MMJJ_RS05935: MMJJ_RS05935 - Era-like GTP-binding protein, at 1,104,236 to 1,104,790
_RS05935
Position (kb)
1102
1103
1104 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1102.072 kb on - strand, within MMJJ_RS05915 at 1102.073 kb on + strand, within MMJJ_RS05915 at 1102.114 kb on + strand at 1102.122 kb on - strand at 1102.159 kb on - strand at 1102.164 kb on - strand at 1102.164 kb on - strand at 1102.176 kb on - strand at 1102.214 kb on + strand at 1102.214 kb on + strand at 1102.260 kb on - strand, within MMJJ_RS05920 at 1102.362 kb on + strand, within MMJJ_RS05920 at 1102.604 kb on - strand, within MMJJ_RS05920 at 1102.798 kb on + strand at 1102.949 kb on - strand at 1102.992 kb on - strand at 1103.007 kb on + strand at 1103.024 kb on - strand at 1103.117 kb on - strand, within MMJJ_RS05925 at 1103.117 kb on - strand, within MMJJ_RS05925 at 1103.163 kb on - strand, within MMJJ_RS05925 at 1103.171 kb on - strand, within MMJJ_RS05925 at 1103.173 kb on - strand, within MMJJ_RS05925 at 1103.173 kb on - strand, within MMJJ_RS05925 at 1103.174 kb on + strand, within MMJJ_RS05925 at 1103.174 kb on + strand, within MMJJ_RS05925 at 1103.174 kb on + strand, within MMJJ_RS05925 at 1103.178 kb on - strand, within MMJJ_RS05925 at 1103.178 kb on - strand, within MMJJ_RS05925 at 1103.183 kb on + strand, within MMJJ_RS05925 at 1103.216 kb on - strand, within MMJJ_RS05925 at 1103.216 kb on - strand, within MMJJ_RS05925 at 1103.217 kb on + strand, within MMJJ_RS05925 at 1103.241 kb on - strand, within MMJJ_RS05925 at 1103.266 kb on + strand, within MMJJ_RS05925 at 1103.272 kb on + strand, within MMJJ_RS05925 at 1103.272 kb on + strand, within MMJJ_RS05925 at 1103.314 kb on - strand, within MMJJ_RS05925 at 1103.324 kb on - strand, within MMJJ_RS05925 at 1103.338 kb on + strand, within MMJJ_RS05925 at 1103.412 kb on - strand, within MMJJ_RS05925 at 1103.422 kb on + strand, within MMJJ_RS05925 at 1103.439 kb on + strand, within MMJJ_RS05925 at 1103.492 kb on - strand, within MMJJ_RS05925 at 1103.515 kb on + strand, within MMJJ_RS05925 at 1103.601 kb on - strand, within MMJJ_RS05925 at 1103.729 kb on - strand, within MMJJ_RS05925 at 1103.818 kb on - strand at 1103.832 kb on - strand at 1103.833 kb on + strand at 1103.837 kb on - strand at 1103.837 kb on - strand at 1103.838 kb on + strand at 1103.838 kb on + strand at 1103.841 kb on - strand at 1103.842 kb on + strand at 1103.842 kb on + strand at 1103.842 kb on + strand at 1103.873 kb on - strand at 1103.893 kb on - strand, within MMJJ_RS05930 at 1103.893 kb on - strand, within MMJJ_RS05930 at 1103.894 kb on + strand, within MMJJ_RS05930 at 1104.065 kb on - strand, within MMJJ_RS05930 at 1104.067 kb on - strand at 1104.152 kb on - strand, within MMJJ_RS05930 at 1104.167 kb on + strand at 1104.255 kb on + strand at 1104.262 kb on - strand at 1104.267 kb on + strand at 1104.269 kb on - strand at 1104.269 kb on - strand at 1104.270 kb on + strand at 1104.385 kb on - strand, within MMJJ_RS05935 at 1104.395 kb on - strand, within MMJJ_RS05935 at 1104.399 kb on + strand at 1104.459 kb on + strand, within MMJJ_RS05935 at 1104.557 kb on + strand, within MMJJ_RS05935 at 1104.638 kb on + strand, within MMJJ_RS05935 at 1104.773 kb on + strand at 1104.813 kb on - strand at 1104.813 kb on - strand at 1104.813 kb on - strand at 1104.814 kb on + strand at 1104.815 kb on - strand at 1104.815 kb on - strand at 1104.816 kb on + strand at 1104.816 kb on + strand at 1104.816 kb on + strand at 1104.816 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Rifampicin 0.25 mM remove 1,102,072 - MMJJ_RS05915 0.81 -0.2 1,102,073 + MMJJ_RS05915 0.81 +1.9 1,102,114 + -1.0 1,102,122 - -1.8 1,102,159 - +1.1 1,102,164 - +0.7 1,102,164 - -0.8 1,102,176 - -0.1 1,102,214 + +0.6 1,102,214 + -1.3 1,102,260 - MMJJ_RS05920 0.12 -2.6 1,102,362 + MMJJ_RS05920 0.25 -1.5 1,102,604 - MMJJ_RS05920 0.56 +0.6 1,102,798 + +0.7 1,102,949 - -2.8 1,102,992 - -2.9 1,103,007 + -0.3 1,103,024 - -1.2 1,103,117 - MMJJ_RS05925 0.18 +0.1 1,103,117 - MMJJ_RS05925 0.18 +0.6 1,103,163 - MMJJ_RS05925 0.23 -1.1 1,103,171 - MMJJ_RS05925 0.24 +1.1 1,103,173 - MMJJ_RS05925 0.24 -0.5 1,103,173 - MMJJ_RS05925 0.24 +1.4 1,103,174 + MMJJ_RS05925 0.25 +2.2 1,103,174 + MMJJ_RS05925 0.25 -0.1 1,103,174 + MMJJ_RS05925 0.25 +0.2 1,103,178 - MMJJ_RS05925 0.25 +0.4 1,103,178 - MMJJ_RS05925 0.25 +1.0 1,103,183 + MMJJ_RS05925 0.26 +0.5 1,103,216 - MMJJ_RS05925 0.29 -0.6 1,103,216 - MMJJ_RS05925 0.29 -0.9 1,103,217 + MMJJ_RS05925 0.30 -0.2 1,103,241 - MMJJ_RS05925 0.32 +0.3 1,103,266 + MMJJ_RS05925 0.35 +0.2 1,103,272 + MMJJ_RS05925 0.36 -3.3 1,103,272 + MMJJ_RS05925 0.36 -0.1 1,103,314 - MMJJ_RS05925 0.41 -0.4 1,103,324 - MMJJ_RS05925 0.42 -2.4 1,103,338 + MMJJ_RS05925 0.44 +0.6 1,103,412 - MMJJ_RS05925 0.52 +1.1 1,103,422 + MMJJ_RS05925 0.54 -0.4 1,103,439 + MMJJ_RS05925 0.56 +2.3 1,103,492 - MMJJ_RS05925 0.62 +2.2 1,103,515 + MMJJ_RS05925 0.65 -1.6 1,103,601 - MMJJ_RS05925 0.75 +0.5 1,103,729 - MMJJ_RS05925 0.90 +1.5 1,103,818 - -2.7 1,103,832 - +0.8 1,103,833 + +2.2 1,103,837 - +1.4 1,103,837 - -0.3 1,103,838 + -1.5 1,103,838 + +1.7 1,103,841 - -1.8 1,103,842 + -1.6 1,103,842 + -1.8 1,103,842 + -0.0 1,103,873 - -3.3 1,103,893 - MMJJ_RS05930 0.14 -2.6 1,103,893 - MMJJ_RS05930 0.14 -0.3 1,103,894 + MMJJ_RS05930 0.14 +1.2 1,104,065 - MMJJ_RS05930 0.58 -0.8 1,104,067 - +2.2 1,104,152 - MMJJ_RS05930 0.80 -0.8 1,104,167 + +1.2 1,104,255 + -0.3 1,104,262 - +1.2 1,104,267 + -0.1 1,104,269 - -2.6 1,104,269 - +0.2 1,104,270 + -0.3 1,104,385 - MMJJ_RS05935 0.27 -0.6 1,104,395 - MMJJ_RS05935 0.29 +2.2 1,104,399 + +0.6 1,104,459 + MMJJ_RS05935 0.40 -2.6 1,104,557 + MMJJ_RS05935 0.58 -2.0 1,104,638 + MMJJ_RS05935 0.72 -1.2 1,104,773 + +0.2 1,104,813 - -1.8 1,104,813 - -0.4 1,104,813 - +1.0 1,104,814 + -0.8 1,104,815 - -0.7 1,104,815 - +0.2 1,104,816 + +0.4 1,104,816 + +0.3 1,104,816 + -0.5 1,104,816 + -2.6
Or see this region's nucleotide sequence