Experiment: McCas_formate_complete Rifampicin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03390 and MMJJ_RS09295 are separated by 7 nucleotides MMJJ_RS09295 and MMJJ_RS03395 are separated by 24 nucleotides MMJJ_RS03395 and MMJJ_RS09300 are separated by 12 nucleotides MMJJ_RS09300 and MMJJ_RS03400 are separated by 11 nucleotides MMJJ_RS03400 and MMJJ_RS03405 overlap by 8 nucleotides MMJJ_RS03405 and MMJJ_RS03410 overlap by 8 nucleotides MMJJ_RS03410 and MMJJ_RS03415 are separated by 3 nucleotides MMJJ_RS03415 and MMJJ_RS03420 overlap by 8 nucleotides
MMJJ_RS03390: MMJJ_RS03390 - hypothetical protein, at 639,826 to 640,062
_RS03390
MMJJ_RS09295: MMJJ_RS09295 - hypothetical protein, at 640,070 to 640,225
_RS09295
MMJJ_RS03395: MMJJ_RS03395 - hypothetical protein, at 640,250 to 640,474
_RS03395
MMJJ_RS09300: MMJJ_RS09300 - hypothetical protein, at 640,487 to 640,660
_RS09300
MMJJ_RS03400: MMJJ_RS03400 - hypothetical protein, at 640,672 to 640,908
_RS03400
MMJJ_RS03405: MMJJ_RS03405 - hypothetical protein, at 640,901 to 642,241
_RS03405
MMJJ_RS03410: MMJJ_RS03410 - hypothetical protein, at 642,234 to 642,650
_RS03410
MMJJ_RS03415: MMJJ_RS03415 - hypothetical protein, at 642,654 to 643,091
_RS03415
MMJJ_RS03420: MMJJ_RS03420 - hypothetical protein, at 643,084 to 643,359
_RS03420
Position (kb)
640
641
642
643 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 640.071 kb on - strand at 640.072 kb on + strand at 640.075 kb on - strand at 640.075 kb on - strand at 640.075 kb on - strand at 640.075 kb on - strand at 640.075 kb on - strand at 640.075 kb on - strand at 640.077 kb on - strand at 640.111 kb on - strand, within MMJJ_RS09295 at 640.330 kb on - strand, within MMJJ_RS03395 at 640.330 kb on - strand, within MMJJ_RS03395 at 640.332 kb on - strand, within MMJJ_RS03395 at 640.332 kb on - strand, within MMJJ_RS03395 at 640.333 kb on + strand, within MMJJ_RS03395 at 640.456 kb on - strand at 640.457 kb on + strand at 640.462 kb on - strand at 640.488 kb on - strand at 640.488 kb on - strand at 640.489 kb on + strand at 640.500 kb on + strand at 640.577 kb on - strand, within MMJJ_RS09300 at 640.640 kb on - strand, within MMJJ_RS09300 at 641.073 kb on - strand, within MMJJ_RS03405 at 641.073 kb on - strand, within MMJJ_RS03405 at 641.162 kb on + strand, within MMJJ_RS03405 at 641.218 kb on + strand, within MMJJ_RS03405 at 641.234 kb on - strand, within MMJJ_RS03405 at 641.236 kb on - strand, within MMJJ_RS03405 at 641.236 kb on - strand, within MMJJ_RS03405 at 641.237 kb on + strand, within MMJJ_RS03405 at 641.348 kb on + strand, within MMJJ_RS03405 at 641.349 kb on - strand, within MMJJ_RS03405 at 641.349 kb on - strand, within MMJJ_RS03405 at 641.349 kb on - strand, within MMJJ_RS03405 at 641.370 kb on + strand, within MMJJ_RS03405 at 641.370 kb on + strand, within MMJJ_RS03405 at 641.370 kb on + strand, within MMJJ_RS03405 at 641.372 kb on - strand, within MMJJ_RS03405 at 641.373 kb on + strand, within MMJJ_RS03405 at 641.374 kb on - strand, within MMJJ_RS03405 at 641.374 kb on - strand, within MMJJ_RS03405 at 641.374 kb on - strand, within MMJJ_RS03405 at 641.374 kb on - strand, within MMJJ_RS03405 at 641.375 kb on + strand, within MMJJ_RS03405 at 641.375 kb on + strand, within MMJJ_RS03405 at 641.375 kb on + strand, within MMJJ_RS03405 at 641.375 kb on + strand, within MMJJ_RS03405 at 641.375 kb on + strand, within MMJJ_RS03405 at 641.422 kb on - strand, within MMJJ_RS03405 at 641.423 kb on + strand, within MMJJ_RS03405 at 641.423 kb on + strand, within MMJJ_RS03405 at 641.438 kb on + strand, within MMJJ_RS03405 at 641.487 kb on + strand, within MMJJ_RS03405 at 641.509 kb on - strand, within MMJJ_RS03405 at 641.510 kb on + strand, within MMJJ_RS03405 at 641.513 kb on + strand, within MMJJ_RS03405 at 641.513 kb on + strand, within MMJJ_RS03405 at 641.513 kb on + strand, within MMJJ_RS03405 at 641.514 kb on - strand, within MMJJ_RS03405 at 641.517 kb on - strand, within MMJJ_RS03405 at 641.607 kb on + strand, within MMJJ_RS03405 at 641.697 kb on + strand at 641.728 kb on + strand, within MMJJ_RS03405 at 641.735 kb on + strand, within MMJJ_RS03405 at 641.735 kb on + strand, within MMJJ_RS03405 at 641.737 kb on + strand, within MMJJ_RS03405 at 641.777 kb on + strand, within MMJJ_RS03405 at 641.789 kb on - strand, within MMJJ_RS03405 at 641.879 kb on - strand, within MMJJ_RS03405 at 641.903 kb on + strand, within MMJJ_RS03405 at 641.907 kb on - strand, within MMJJ_RS03405 at 642.103 kb on + strand, within MMJJ_RS03405 at 642.161 kb on - strand at 642.208 kb on + strand at 642.238 kb on + strand at 642.238 kb on + strand at 642.285 kb on - strand, within MMJJ_RS03410 at 642.329 kb on - strand, within MMJJ_RS03410 at 642.368 kb on - strand, within MMJJ_RS03410 at 642.420 kb on - strand, within MMJJ_RS03410 at 642.508 kb on - strand, within MMJJ_RS03410 at 642.540 kb on - strand, within MMJJ_RS03410 at 642.595 kb on - strand, within MMJJ_RS03410 at 642.656 kb on + strand at 642.734 kb on + strand, within MMJJ_RS03415 at 642.741 kb on - strand, within MMJJ_RS03415 at 642.795 kb on + strand, within MMJJ_RS03415 at 642.821 kb on - strand, within MMJJ_RS03415 at 642.821 kb on - strand, within MMJJ_RS03415 at 642.873 kb on - strand, within MMJJ_RS03415 at 642.891 kb on - strand, within MMJJ_RS03415 at 642.899 kb on + strand, within MMJJ_RS03415 at 642.899 kb on + strand, within MMJJ_RS03415 at 642.951 kb on - strand, within MMJJ_RS03415 at 642.951 kb on - strand, within MMJJ_RS03415 at 642.951 kb on - strand, within MMJJ_RS03415 at 642.957 kb on - strand, within MMJJ_RS03415 at 642.958 kb on + strand, within MMJJ_RS03415 at 642.988 kb on - strand, within MMJJ_RS03415 at 643.065 kb on + strand at 643.071 kb on + strand at 643.071 kb on + strand at 643.108 kb on - strand at 643.116 kb on + strand, within MMJJ_RS03420 at 643.116 kb on + strand, within MMJJ_RS03420 at 643.116 kb on + strand, within MMJJ_RS03420 at 643.133 kb on - strand, within MMJJ_RS03420 at 643.134 kb on + strand, within MMJJ_RS03420 at 643.134 kb on + strand, within MMJJ_RS03420 at 643.188 kb on + strand, within MMJJ_RS03420 at 643.213 kb on - strand, within MMJJ_RS03420 at 643.214 kb on + strand, within MMJJ_RS03420 at 643.214 kb on + strand, within MMJJ_RS03420 at 643.214 kb on + strand, within MMJJ_RS03420 at 643.217 kb on - strand, within MMJJ_RS03420 at 643.217 kb on - strand, within MMJJ_RS03420 at 643.218 kb on + strand, within MMJJ_RS03420 at 643.232 kb on + strand, within MMJJ_RS03420 at 643.238 kb on - strand, within MMJJ_RS03420 at 643.239 kb on + strand, within MMJJ_RS03420
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Rifampicin 0.25 mM remove 640,071 - +0.5 640,072 + +3.1 640,075 - +0.0 640,075 - +1.2 640,075 - +0.0 640,075 - -1.6 640,075 - -1.0 640,075 - +0.6 640,077 - +0.2 640,111 - MMJJ_RS09295 0.26 -2.5 640,330 - MMJJ_RS03395 0.36 -0.7 640,330 - MMJJ_RS03395 0.36 -0.3 640,332 - MMJJ_RS03395 0.36 +2.2 640,332 - MMJJ_RS03395 0.36 -1.3 640,333 + MMJJ_RS03395 0.37 -0.1 640,456 - -1.1 640,457 + -0.2 640,462 - +1.4 640,488 - +1.1 640,488 - -3.1 640,489 + -3.4 640,500 + +1.6 640,577 - MMJJ_RS09300 0.52 -0.1 640,640 - MMJJ_RS09300 0.88 -1.9 641,073 - MMJJ_RS03405 0.13 +1.5 641,073 - MMJJ_RS03405 0.13 +0.3 641,162 + MMJJ_RS03405 0.19 +1.7 641,218 + MMJJ_RS03405 0.24 +1.7 641,234 - MMJJ_RS03405 0.25 +0.2 641,236 - MMJJ_RS03405 0.25 +2.1 641,236 - MMJJ_RS03405 0.25 +2.3 641,237 + MMJJ_RS03405 0.25 -0.2 641,348 + MMJJ_RS03405 0.33 -1.3 641,349 - MMJJ_RS03405 0.33 +2.2 641,349 - MMJJ_RS03405 0.33 +1.9 641,349 - MMJJ_RS03405 0.33 +0.1 641,370 + MMJJ_RS03405 0.35 -1.4 641,370 + MMJJ_RS03405 0.35 -0.9 641,370 + MMJJ_RS03405 0.35 +1.8 641,372 - MMJJ_RS03405 0.35 -0.7 641,373 + MMJJ_RS03405 0.35 -1.0 641,374 - MMJJ_RS03405 0.35 +1.4 641,374 - MMJJ_RS03405 0.35 +0.7 641,374 - MMJJ_RS03405 0.35 +0.3 641,374 - MMJJ_RS03405 0.35 +3.5 641,375 + MMJJ_RS03405 0.35 +0.5 641,375 + MMJJ_RS03405 0.35 -0.5 641,375 + MMJJ_RS03405 0.35 -0.1 641,375 + MMJJ_RS03405 0.35 -0.7 641,375 + MMJJ_RS03405 0.35 -2.6 641,422 - MMJJ_RS03405 0.39 +1.1 641,423 + MMJJ_RS03405 0.39 +1.7 641,423 + MMJJ_RS03405 0.39 -1.9 641,438 + MMJJ_RS03405 0.40 -0.6 641,487 + MMJJ_RS03405 0.44 +3.7 641,509 - MMJJ_RS03405 0.45 -1.3 641,510 + MMJJ_RS03405 0.45 +0.1 641,513 + MMJJ_RS03405 0.46 +1.8 641,513 + MMJJ_RS03405 0.46 -3.4 641,513 + MMJJ_RS03405 0.46 -1.0 641,514 - MMJJ_RS03405 0.46 +0.6 641,517 - MMJJ_RS03405 0.46 +0.5 641,607 + MMJJ_RS03405 0.53 -3.8 641,697 + +1.1 641,728 + MMJJ_RS03405 0.62 -1.2 641,735 + MMJJ_RS03405 0.62 +2.8 641,735 + MMJJ_RS03405 0.62 -2.4 641,737 + MMJJ_RS03405 0.62 -0.0 641,777 + MMJJ_RS03405 0.65 -1.2 641,789 - MMJJ_RS03405 0.66 -2.6 641,879 - MMJJ_RS03405 0.73 -1.5 641,903 + MMJJ_RS03405 0.75 +0.5 641,907 - MMJJ_RS03405 0.75 -0.3 642,103 + MMJJ_RS03405 0.90 +0.1 642,161 - -0.2 642,208 + -3.6 642,238 + -1.0 642,238 + -2.3 642,285 - MMJJ_RS03410 0.12 +0.7 642,329 - MMJJ_RS03410 0.23 +0.6 642,368 - MMJJ_RS03410 0.32 +2.2 642,420 - MMJJ_RS03410 0.45 +3.2 642,508 - MMJJ_RS03410 0.66 +0.3 642,540 - MMJJ_RS03410 0.73 +2.3 642,595 - MMJJ_RS03410 0.87 +1.5 642,656 + -0.7 642,734 + MMJJ_RS03415 0.18 +1.9 642,741 - MMJJ_RS03415 0.20 -2.9 642,795 + MMJJ_RS03415 0.32 +1.1 642,821 - MMJJ_RS03415 0.38 -0.1 642,821 - MMJJ_RS03415 0.38 -0.3 642,873 - MMJJ_RS03415 0.50 -2.8 642,891 - MMJJ_RS03415 0.54 +1.5 642,899 + MMJJ_RS03415 0.56 +1.9 642,899 + MMJJ_RS03415 0.56 -0.4 642,951 - MMJJ_RS03415 0.68 -0.7 642,951 - MMJJ_RS03415 0.68 +1.9 642,951 - MMJJ_RS03415 0.68 -0.2 642,957 - MMJJ_RS03415 0.69 -2.1 642,958 + MMJJ_RS03415 0.69 -0.4 642,988 - MMJJ_RS03415 0.76 +0.7 643,065 + -0.4 643,071 + -0.7 643,071 + +0.4 643,108 - -1.8 643,116 + MMJJ_RS03420 0.12 -0.9 643,116 + MMJJ_RS03420 0.12 -2.5 643,116 + MMJJ_RS03420 0.12 +0.7 643,133 - MMJJ_RS03420 0.18 -2.5 643,134 + MMJJ_RS03420 0.18 +1.6 643,134 + MMJJ_RS03420 0.18 +0.1 643,188 + MMJJ_RS03420 0.38 -1.4 643,213 - MMJJ_RS03420 0.47 -1.3 643,214 + MMJJ_RS03420 0.47 -3.3 643,214 + MMJJ_RS03420 0.47 -0.4 643,214 + MMJJ_RS03420 0.47 -1.8 643,217 - MMJJ_RS03420 0.48 +1.8 643,217 - MMJJ_RS03420 0.48 +1.7 643,218 + MMJJ_RS03420 0.49 -0.3 643,232 + MMJJ_RS03420 0.54 -0.5 643,238 - MMJJ_RS03420 0.56 -1.6 643,239 + MMJJ_RS03420 0.56 -2.5
Or see this region's nucleotide sequence