Experiment: McCas_formate_complete deta nonoate 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06990 and MMJJ_RS06995 are separated by 84 nucleotides MMJJ_RS06995 and MMJJ_RS07000 are separated by 21 nucleotides
MMJJ_RS06990: MMJJ_RS06990 - adenylosuccinate synthetase, at 1,283,056 to 1,284,072
_RS06990
MMJJ_RS06995: MMJJ_RS06995 - 2-phosphoglycerate kinase, at 1,284,157 to 1,285,095
_RS06995
MMJJ_RS07000: MMJJ_RS07000 - TrkH family potassium uptake protein, at 1,285,117 to 1,286,553
_RS07000
Position (kb)
1284
1285
1286 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1283.519 kb on + strand at 1284.086 kb on + strand at 1284.089 kb on - strand at 1284.102 kb on - strand at 1284.103 kb on + strand at 1284.109 kb on + strand at 1284.109 kb on + strand at 1284.111 kb on - strand at 1284.111 kb on - strand at 1284.112 kb on + strand at 1284.112 kb on + strand at 1284.113 kb on - strand at 1284.113 kb on - strand at 1284.113 kb on - strand at 1284.114 kb on + strand at 1284.114 kb on + strand at 1284.114 kb on + strand at 1284.114 kb on + strand at 1284.115 kb on - strand at 1284.115 kb on - strand at 1284.115 kb on - strand at 1284.116 kb on + strand at 1284.116 kb on + strand at 1284.116 kb on + strand at 1284.116 kb on + strand at 1284.118 kb on - strand at 1284.118 kb on - strand at 1284.119 kb on + strand at 1284.122 kb on + strand at 1284.153 kb on - strand at 1284.154 kb on + strand at 1284.171 kb on - strand at 1284.171 kb on - strand at 1284.172 kb on + strand at 1284.174 kb on - strand at 1284.175 kb on + strand at 1284.175 kb on + strand at 1284.175 kb on + strand at 1284.185 kb on - strand at 1284.195 kb on - strand at 1284.195 kb on - strand at 1284.195 kb on - strand at 1284.274 kb on - strand, within MMJJ_RS06995 at 1284.275 kb on + strand, within MMJJ_RS06995 at 1284.276 kb on - strand, within MMJJ_RS06995 at 1284.276 kb on - strand, within MMJJ_RS06995 at 1284.277 kb on + strand, within MMJJ_RS06995 at 1284.282 kb on + strand, within MMJJ_RS06995 at 1284.305 kb on - strand, within MMJJ_RS06995 at 1284.306 kb on + strand, within MMJJ_RS06995 at 1284.320 kb on - strand, within MMJJ_RS06995 at 1284.357 kb on + strand, within MMJJ_RS06995 at 1284.358 kb on - strand, within MMJJ_RS06995 at 1284.358 kb on - strand, within MMJJ_RS06995 at 1284.358 kb on - strand, within MMJJ_RS06995 at 1284.358 kb on - strand, within MMJJ_RS06995 at 1284.359 kb on + strand, within MMJJ_RS06995 at 1284.359 kb on + strand, within MMJJ_RS06995 at 1284.359 kb on + strand, within MMJJ_RS06995 at 1284.369 kb on - strand, within MMJJ_RS06995 at 1284.370 kb on + strand, within MMJJ_RS06995 at 1284.411 kb on + strand, within MMJJ_RS06995 at 1284.426 kb on + strand, within MMJJ_RS06995 at 1284.438 kb on - strand, within MMJJ_RS06995 at 1284.439 kb on + strand, within MMJJ_RS06995 at 1284.444 kb on - strand, within MMJJ_RS06995 at 1284.452 kb on - strand, within MMJJ_RS06995 at 1284.453 kb on + strand, within MMJJ_RS06995 at 1284.604 kb on - strand, within MMJJ_RS06995 at 1284.604 kb on - strand, within MMJJ_RS06995 at 1284.607 kb on + strand, within MMJJ_RS06995 at 1284.607 kb on + strand, within MMJJ_RS06995 at 1284.717 kb on - strand, within MMJJ_RS06995 at 1284.722 kb on - strand, within MMJJ_RS06995 at 1284.723 kb on + strand, within MMJJ_RS06995 at 1284.723 kb on + strand, within MMJJ_RS06995 at 1284.726 kb on - strand, within MMJJ_RS06995 at 1284.727 kb on + strand, within MMJJ_RS06995 at 1284.727 kb on + strand, within MMJJ_RS06995 at 1284.735 kb on + strand, within MMJJ_RS06995 at 1284.754 kb on + strand, within MMJJ_RS06995 at 1284.755 kb on - strand, within MMJJ_RS06995 at 1284.773 kb on - strand, within MMJJ_RS06995 at 1284.773 kb on - strand, within MMJJ_RS06995 at 1284.773 kb on - strand, within MMJJ_RS06995 at 1284.773 kb on - strand, within MMJJ_RS06995 at 1284.773 kb on - strand, within MMJJ_RS06995 at 1284.774 kb on + strand, within MMJJ_RS06995 at 1284.774 kb on + strand, within MMJJ_RS06995 at 1284.819 kb on - strand, within MMJJ_RS06995 at 1284.828 kb on + strand, within MMJJ_RS06995 at 1284.887 kb on - strand, within MMJJ_RS06995 at 1284.933 kb on + strand, within MMJJ_RS06995 at 1284.949 kb on - strand, within MMJJ_RS06995 at 1284.949 kb on - strand, within MMJJ_RS06995 at 1284.951 kb on - strand, within MMJJ_RS06995 at 1285.050 kb on - strand at 1285.059 kb on + strand at 1285.059 kb on + strand at 1285.094 kb on + strand at 1285.094 kb on + strand at 1285.669 kb on - strand, within MMJJ_RS07000 at 1285.904 kb on - strand, within MMJJ_RS07000 at 1286.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete deta nonoate 0.025 mM remove 1,283,519 + -0.3 1,284,086 + -0.3 1,284,089 - -2.8 1,284,102 - -0.5 1,284,103 + -2.1 1,284,109 + -1.0 1,284,109 + +1.1 1,284,111 - +0.3 1,284,111 - -1.5 1,284,112 + -2.8 1,284,112 + -0.2 1,284,113 - -0.3 1,284,113 - -2.3 1,284,113 - -1.8 1,284,114 + +0.3 1,284,114 + -2.9 1,284,114 + +0.0 1,284,114 + -0.3 1,284,115 - -0.4 1,284,115 - -2.8 1,284,115 - +0.4 1,284,116 + -1.1 1,284,116 + -1.6 1,284,116 + -0.1 1,284,116 + +0.4 1,284,118 - +0.4 1,284,118 - -0.0 1,284,119 + -1.5 1,284,122 + -1.9 1,284,153 - -1.1 1,284,154 + -0.8 1,284,171 - -1.0 1,284,171 - +2.0 1,284,172 + -1.5 1,284,174 - -1.6 1,284,175 + -2.3 1,284,175 + -1.6 1,284,175 + -3.4 1,284,185 - -2.3 1,284,195 - -0.4 1,284,195 - -1.3 1,284,195 - -1.7 1,284,274 - MMJJ_RS06995 0.12 -3.1 1,284,275 + MMJJ_RS06995 0.13 -1.6 1,284,276 - MMJJ_RS06995 0.13 -3.0 1,284,276 - MMJJ_RS06995 0.13 +0.0 1,284,277 + MMJJ_RS06995 0.13 -2.0 1,284,282 + MMJJ_RS06995 0.13 -2.4 1,284,305 - MMJJ_RS06995 0.16 -1.2 1,284,306 + MMJJ_RS06995 0.16 -2.2 1,284,320 - MMJJ_RS06995 0.17 -1.9 1,284,357 + MMJJ_RS06995 0.21 -2.2 1,284,358 - MMJJ_RS06995 0.21 -1.6 1,284,358 - MMJJ_RS06995 0.21 +0.3 1,284,358 - MMJJ_RS06995 0.21 -0.5 1,284,358 - MMJJ_RS06995 0.21 -2.1 1,284,359 + MMJJ_RS06995 0.22 -4.2 1,284,359 + MMJJ_RS06995 0.22 -2.0 1,284,359 + MMJJ_RS06995 0.22 -3.0 1,284,369 - MMJJ_RS06995 0.23 -3.5 1,284,370 + MMJJ_RS06995 0.23 +1.0 1,284,411 + MMJJ_RS06995 0.27 -3.1 1,284,426 + MMJJ_RS06995 0.29 +0.3 1,284,438 - MMJJ_RS06995 0.30 -2.9 1,284,439 + MMJJ_RS06995 0.30 -1.0 1,284,444 - MMJJ_RS06995 0.31 -2.9 1,284,452 - MMJJ_RS06995 0.31 -1.2 1,284,453 + MMJJ_RS06995 0.32 -1.3 1,284,604 - MMJJ_RS06995 0.48 -3.6 1,284,604 - MMJJ_RS06995 0.48 -1.7 1,284,607 + MMJJ_RS06995 0.48 -2.3 1,284,607 + MMJJ_RS06995 0.48 -2.5 1,284,717 - MMJJ_RS06995 0.60 -2.9 1,284,722 - MMJJ_RS06995 0.60 +1.2 1,284,723 + MMJJ_RS06995 0.60 -0.9 1,284,723 + MMJJ_RS06995 0.60 -2.9 1,284,726 - MMJJ_RS06995 0.61 -1.0 1,284,727 + MMJJ_RS06995 0.61 -0.8 1,284,727 + MMJJ_RS06995 0.61 -3.0 1,284,735 + MMJJ_RS06995 0.62 +0.2 1,284,754 + MMJJ_RS06995 0.64 -1.2 1,284,755 - MMJJ_RS06995 0.64 -2.4 1,284,773 - MMJJ_RS06995 0.66 -1.4 1,284,773 - MMJJ_RS06995 0.66 -1.8 1,284,773 - MMJJ_RS06995 0.66 -0.9 1,284,773 - MMJJ_RS06995 0.66 -3.3 1,284,773 - MMJJ_RS06995 0.66 -3.0 1,284,774 + MMJJ_RS06995 0.66 -2.9 1,284,774 + MMJJ_RS06995 0.66 -1.9 1,284,819 - MMJJ_RS06995 0.71 -1.2 1,284,828 + MMJJ_RS06995 0.71 -2.0 1,284,887 - MMJJ_RS06995 0.78 -0.3 1,284,933 + MMJJ_RS06995 0.83 -1.8 1,284,949 - MMJJ_RS06995 0.84 +0.0 1,284,949 - MMJJ_RS06995 0.84 -1.0 1,284,951 - MMJJ_RS06995 0.85 -1.1 1,285,050 - -1.0 1,285,059 + -3.1 1,285,059 + +1.0 1,285,094 + -1.0 1,285,094 + -1.2 1,285,669 - MMJJ_RS07000 0.38 -0.5 1,285,904 - MMJJ_RS07000 0.55 +1.0 1,286,058 + +0.5
Or see this region's nucleotide sequence