Experiment: McCas_formate_complete deta nonoate 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05795 and MMJJ_RS05800 are separated by 31 nucleotides MMJJ_RS05800 and MMJJ_RS05805 are separated by 50 nucleotides MMJJ_RS05805 and MMJJ_RS05810 are separated by 29 nucleotides MMJJ_RS05810 and MMJJ_RS05815 overlap by 4 nucleotides MMJJ_RS05815 and MMJJ_RS05820 overlap by 11 nucleotides
MMJJ_RS05795: MMJJ_RS05795 - hypothetical protein, at 1,079,227 to 1,080,906
_RS05795
MMJJ_RS05800: MMJJ_RS05800 - coenzyme F420-0:L-glutamate ligase, at 1,080,938 to 1,081,834
_RS05800
MMJJ_RS05805: MMJJ_RS05805 - aconitase X catalytic domain-containing protein, at 1,081,885 to 1,083,090
_RS05805
MMJJ_RS05810: MMJJ_RS05810 - precorrin-4 C(11)-methyltransferase, at 1,083,120 to 1,083,884
_RS05810
MMJJ_RS05815: MMJJ_RS05815 - hypothetical protein, at 1,083,881 to 1,084,078
_RS05815
MMJJ_RS05820: MMJJ_RS05820 - DUF2304 domain-containing protein, at 1,084,068 to 1,084,427
_RS05820
Position (kb)
1081
1082
1083
1084 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1080.904 kb on - strand at 1080.904 kb on - strand at 1080.905 kb on + strand at 1080.905 kb on + strand at 1080.905 kb on + strand at 1080.908 kb on - strand at 1080.908 kb on - strand at 1080.908 kb on - strand at 1080.908 kb on - strand at 1080.909 kb on + strand at 1080.909 kb on + strand at 1080.909 kb on + strand at 1080.909 kb on + strand at 1080.909 kb on + strand at 1080.951 kb on + strand at 1080.955 kb on - strand at 1080.956 kb on + strand at 1080.959 kb on - strand at 1080.959 kb on - strand at 1080.960 kb on + strand at 1081.083 kb on - strand, within MMJJ_RS05800 at 1081.195 kb on - strand, within MMJJ_RS05800 at 1081.195 kb on - strand, within MMJJ_RS05800 at 1081.195 kb on - strand, within MMJJ_RS05800 at 1081.196 kb on + strand, within MMJJ_RS05800 at 1081.196 kb on + strand, within MMJJ_RS05800 at 1081.258 kb on + strand, within MMJJ_RS05800 at 1081.258 kb on + strand, within MMJJ_RS05800 at 1081.258 kb on + strand, within MMJJ_RS05800 at 1081.276 kb on + strand, within MMJJ_RS05800 at 1081.276 kb on + strand, within MMJJ_RS05800 at 1081.289 kb on + strand, within MMJJ_RS05800 at 1081.390 kb on + strand, within MMJJ_RS05800 at 1081.390 kb on + strand, within MMJJ_RS05800 at 1081.448 kb on - strand, within MMJJ_RS05800 at 1081.449 kb on + strand, within MMJJ_RS05800 at 1081.473 kb on + strand, within MMJJ_RS05800 at 1081.514 kb on - strand, within MMJJ_RS05800 at 1081.541 kb on + strand, within MMJJ_RS05800 at 1081.544 kb on - strand, within MMJJ_RS05800 at 1081.545 kb on + strand, within MMJJ_RS05800 at 1081.545 kb on + strand, within MMJJ_RS05800 at 1081.559 kb on - strand, within MMJJ_RS05800 at 1081.559 kb on - strand, within MMJJ_RS05800 at 1081.560 kb on + strand, within MMJJ_RS05800 at 1081.560 kb on + strand, within MMJJ_RS05800 at 1081.563 kb on - strand, within MMJJ_RS05800 at 1081.587 kb on - strand, within MMJJ_RS05800 at 1081.587 kb on - strand, within MMJJ_RS05800 at 1081.587 kb on - strand, within MMJJ_RS05800 at 1081.588 kb on + strand, within MMJJ_RS05800 at 1081.588 kb on + strand, within MMJJ_RS05800 at 1081.590 kb on - strand, within MMJJ_RS05800 at 1081.591 kb on + strand, within MMJJ_RS05800 at 1081.591 kb on + strand, within MMJJ_RS05800 at 1081.634 kb on - strand, within MMJJ_RS05800 at 1081.654 kb on - strand, within MMJJ_RS05800 at 1081.654 kb on - strand, within MMJJ_RS05800 at 1081.655 kb on + strand, within MMJJ_RS05800 at 1081.659 kb on + strand, within MMJJ_RS05800 at 1081.771 kb on - strand at 1081.772 kb on + strand at 1081.774 kb on + strand at 1081.774 kb on + strand at 1081.800 kb on + strand at 1081.820 kb on - strand at 1081.821 kb on + strand at 1081.821 kb on + strand at 1081.827 kb on + strand at 1081.827 kb on + strand at 1081.827 kb on + strand at 1081.841 kb on - strand at 1081.857 kb on + strand at 1081.857 kb on + strand at 1081.857 kb on + strand at 1081.858 kb on - strand at 1081.858 kb on - strand at 1081.859 kb on + strand at 1081.859 kb on + strand at 1081.859 kb on + strand at 1081.859 kb on + strand at 1081.863 kb on + strand at 1081.866 kb on + strand at 1082.006 kb on - strand, within MMJJ_RS05805 at 1082.315 kb on + strand, within MMJJ_RS05805 at 1082.639 kb on - strand, within MMJJ_RS05805 at 1082.688 kb on + strand at 1082.814 kb on + strand, within MMJJ_RS05805 at 1083.207 kb on + strand, within MMJJ_RS05810 at 1083.211 kb on - strand, within MMJJ_RS05810 at 1083.212 kb on + strand, within MMJJ_RS05810 at 1083.212 kb on + strand at 1083.212 kb on + strand, within MMJJ_RS05810 at 1083.332 kb on - strand, within MMJJ_RS05810 at 1083.344 kb on + strand, within MMJJ_RS05810 at 1083.509 kb on + strand, within MMJJ_RS05810 at 1083.520 kb on - strand, within MMJJ_RS05810 at 1083.523 kb on - strand, within MMJJ_RS05810 at 1083.523 kb on - strand at 1083.597 kb on + strand, within MMJJ_RS05810 at 1083.641 kb on - strand, within MMJJ_RS05810 at 1083.642 kb on + strand, within MMJJ_RS05810 at 1083.642 kb on + strand, within MMJJ_RS05810 at 1083.649 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.691 kb on - strand, within MMJJ_RS05810 at 1083.692 kb on + strand, within MMJJ_RS05810 at 1083.693 kb on - strand, within MMJJ_RS05810 at 1083.737 kb on + strand, within MMJJ_RS05810 at 1083.745 kb on - strand, within MMJJ_RS05810 at 1083.788 kb on - strand, within MMJJ_RS05810 at 1083.789 kb on + strand, within MMJJ_RS05810 at 1083.789 kb on + strand at 1083.790 kb on - strand, within MMJJ_RS05810 at 1083.790 kb on - strand at 1083.822 kb on - strand at 1083.823 kb on + strand at 1083.891 kb on + strand at 1083.899 kb on - strand at 1083.912 kb on + strand, within MMJJ_RS05815 at 1083.953 kb on + strand, within MMJJ_RS05815 at 1083.953 kb on + strand, within MMJJ_RS05815 at 1083.956 kb on - strand, within MMJJ_RS05815 at 1083.956 kb on - strand, within MMJJ_RS05815 at 1083.980 kb on - strand, within MMJJ_RS05815 at 1084.021 kb on - strand, within MMJJ_RS05815 at 1084.021 kb on - strand, within MMJJ_RS05815 at 1084.022 kb on + strand, within MMJJ_RS05815 at 1084.061 kb on - strand at 1084.062 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete deta nonoate 0.025 mM remove 1,080,904 - -2.0 1,080,904 - +0.3 1,080,905 + -0.8 1,080,905 + +0.9 1,080,905 + +1.5 1,080,908 - +2.9 1,080,908 - -0.4 1,080,908 - -0.7 1,080,908 - +1.5 1,080,909 + -0.2 1,080,909 + +0.1 1,080,909 + +0.7 1,080,909 + +1.5 1,080,909 + -0.1 1,080,951 + -1.0 1,080,955 - -0.7 1,080,956 + -1.8 1,080,959 - -0.7 1,080,959 - +0.3 1,080,960 + -1.7 1,081,083 - MMJJ_RS05800 0.16 -1.6 1,081,195 - MMJJ_RS05800 0.29 -1.9 1,081,195 - MMJJ_RS05800 0.29 -2.0 1,081,195 - MMJJ_RS05800 0.29 -1.2 1,081,196 + MMJJ_RS05800 0.29 +2.1 1,081,196 + MMJJ_RS05800 0.29 +0.4 1,081,258 + MMJJ_RS05800 0.36 -1.7 1,081,258 + MMJJ_RS05800 0.36 -2.8 1,081,258 + MMJJ_RS05800 0.36 -0.0 1,081,276 + MMJJ_RS05800 0.38 -0.4 1,081,276 + MMJJ_RS05800 0.38 -1.5 1,081,289 + MMJJ_RS05800 0.39 -3.2 1,081,390 + MMJJ_RS05800 0.50 -0.6 1,081,390 + MMJJ_RS05800 0.50 -2.1 1,081,448 - MMJJ_RS05800 0.57 -0.1 1,081,449 + MMJJ_RS05800 0.57 -0.7 1,081,473 + MMJJ_RS05800 0.60 -0.3 1,081,514 - MMJJ_RS05800 0.64 -1.6 1,081,541 + MMJJ_RS05800 0.67 -1.1 1,081,544 - MMJJ_RS05800 0.68 -2.1 1,081,545 + MMJJ_RS05800 0.68 -0.1 1,081,545 + MMJJ_RS05800 0.68 -0.8 1,081,559 - MMJJ_RS05800 0.69 -0.5 1,081,559 - MMJJ_RS05800 0.69 -0.6 1,081,560 + MMJJ_RS05800 0.69 -1.4 1,081,560 + MMJJ_RS05800 0.69 -1.0 1,081,563 - MMJJ_RS05800 0.70 -1.9 1,081,587 - MMJJ_RS05800 0.72 +0.3 1,081,587 - MMJJ_RS05800 0.72 -1.9 1,081,587 - MMJJ_RS05800 0.72 -1.6 1,081,588 + MMJJ_RS05800 0.72 -2.1 1,081,588 + MMJJ_RS05800 0.72 -0.9 1,081,590 - MMJJ_RS05800 0.73 +2.6 1,081,591 + MMJJ_RS05800 0.73 -1.3 1,081,591 + MMJJ_RS05800 0.73 -0.8 1,081,634 - MMJJ_RS05800 0.78 -1.7 1,081,654 - MMJJ_RS05800 0.80 -1.1 1,081,654 - MMJJ_RS05800 0.80 -0.4 1,081,655 + MMJJ_RS05800 0.80 -1.6 1,081,659 + MMJJ_RS05800 0.80 -0.4 1,081,771 - -0.6 1,081,772 + -0.9 1,081,774 + -0.8 1,081,774 + -3.0 1,081,800 + -1.4 1,081,820 - +0.2 1,081,821 + -0.2 1,081,821 + +0.1 1,081,827 + +1.0 1,081,827 + -2.6 1,081,827 + -0.2 1,081,841 - +2.3 1,081,857 + -0.5 1,081,857 + -1.8 1,081,857 + -0.0 1,081,858 - -0.8 1,081,858 - +0.4 1,081,859 + +0.4 1,081,859 + -0.8 1,081,859 + -0.0 1,081,859 + -0.6 1,081,863 + -0.8 1,081,866 + +0.6 1,082,006 - MMJJ_RS05805 0.10 +0.4 1,082,315 + MMJJ_RS05805 0.36 -0.9 1,082,639 - MMJJ_RS05805 0.63 -1.4 1,082,688 + +0.9 1,082,814 + MMJJ_RS05805 0.77 +0.4 1,083,207 + MMJJ_RS05810 0.11 -2.6 1,083,211 - MMJJ_RS05810 0.12 -0.4 1,083,212 + MMJJ_RS05810 0.12 -0.4 1,083,212 + +0.9 1,083,212 + MMJJ_RS05810 0.12 -2.1 1,083,332 - MMJJ_RS05810 0.28 -0.6 1,083,344 + MMJJ_RS05810 0.29 -1.2 1,083,509 + MMJJ_RS05810 0.51 +0.9 1,083,520 - MMJJ_RS05810 0.52 -1.5 1,083,523 - MMJJ_RS05810 0.53 +0.4 1,083,523 - -0.4 1,083,597 + MMJJ_RS05810 0.62 -3.1 1,083,641 - MMJJ_RS05810 0.68 -0.1 1,083,642 + MMJJ_RS05810 0.68 -1.4 1,083,642 + MMJJ_RS05810 0.68 -0.6 1,083,649 - MMJJ_RS05810 0.69 -2.3 1,083,691 - MMJJ_RS05810 0.75 -2.1 1,083,691 - MMJJ_RS05810 0.75 +0.4 1,083,691 - MMJJ_RS05810 0.75 -2.1 1,083,692 + MMJJ_RS05810 0.75 +0.9 1,083,693 - MMJJ_RS05810 0.75 -2.1 1,083,737 + MMJJ_RS05810 0.81 -3.3 1,083,745 - MMJJ_RS05810 0.82 +0.9 1,083,788 - MMJJ_RS05810 0.87 +1.0 1,083,789 + MMJJ_RS05810 0.87 -1.1 1,083,789 + -0.4 1,083,790 - MMJJ_RS05810 0.88 -2.5 1,083,790 - +0.3 1,083,822 - -0.4 1,083,823 + -2.1 1,083,891 + -0.9 1,083,899 - -0.1 1,083,912 + MMJJ_RS05815 0.16 +0.1 1,083,953 + MMJJ_RS05815 0.36 +2.2 1,083,953 + MMJJ_RS05815 0.36 -0.3 1,083,956 - MMJJ_RS05815 0.38 +1.3 1,083,956 - MMJJ_RS05815 0.38 -1.9 1,083,980 - MMJJ_RS05815 0.50 +0.3 1,084,021 - MMJJ_RS05815 0.71 +0.6 1,084,021 - MMJJ_RS05815 0.71 +0.1 1,084,022 + MMJJ_RS05815 0.71 +1.2 1,084,061 - +0.4 1,084,062 + -0.1
Or see this region's nucleotide sequence