Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08870 and MMJJ_RS08875 are separated by 83 nucleotides MMJJ_RS08875 and MMJJ_RS08880 are separated by 30 nucleotides MMJJ_RS08880 and MMJJ_RS08885 are separated by 15 nucleotides
MMJJ_RS08870: MMJJ_RS08870 - 2-isopropylmalate synthase, at 1,634,617 to 1,636,161
_RS08870
MMJJ_RS08875: MMJJ_RS08875 - hypothetical protein, at 1,636,245 to 1,638,089
_RS08875
MMJJ_RS08880: MMJJ_RS08880 - IMPACT family protein, at 1,638,120 to 1,638,728
_RS08880
MMJJ_RS08885: MMJJ_RS08885 - cysteine--tRNA ligase, at 1,638,744 to 1,640,174
_RS08885
Position (kb)
1636
1637
1638
1639 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1636.074 kb on - strand at 1636.075 kb on + strand at 1636.246 kb on - strand at 1636.246 kb on - strand at 1636.247 kb on + strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.268 kb on - strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.269 kb on + strand at 1636.273 kb on + strand at 1636.302 kb on - strand at 1636.303 kb on + strand at 1636.303 kb on + strand at 1636.306 kb on - strand at 1636.306 kb on - strand at 1636.307 kb on + strand at 1636.307 kb on + strand at 1636.317 kb on - strand at 1636.318 kb on + strand at 1636.318 kb on + strand at 1636.318 kb on + strand at 1636.319 kb on - strand at 1636.349 kb on - strand at 1636.386 kb on - strand at 1636.400 kb on - strand at 1636.400 kb on - strand at 1636.403 kb on + strand at 1636.406 kb on - strand at 1636.442 kb on - strand, within MMJJ_RS08875 at 1636.456 kb on - strand, within MMJJ_RS08875 at 1636.469 kb on + strand, within MMJJ_RS08875 at 1636.473 kb on + strand, within MMJJ_RS08875 at 1636.473 kb on + strand, within MMJJ_RS08875 at 1636.525 kb on - strand, within MMJJ_RS08875 at 1636.525 kb on - strand, within MMJJ_RS08875 at 1636.547 kb on - strand, within MMJJ_RS08875 at 1636.549 kb on - strand, within MMJJ_RS08875 at 1636.549 kb on - strand, within MMJJ_RS08875 at 1636.549 kb on - strand, within MMJJ_RS08875 at 1636.550 kb on + strand, within MMJJ_RS08875 at 1636.550 kb on + strand, within MMJJ_RS08875 at 1636.550 kb on + strand, within MMJJ_RS08875 at 1636.572 kb on - strand, within MMJJ_RS08875 at 1636.637 kb on - strand, within MMJJ_RS08875 at 1636.638 kb on + strand, within MMJJ_RS08875 at 1636.640 kb on - strand, within MMJJ_RS08875 at 1636.640 kb on - strand, within MMJJ_RS08875 at 1636.641 kb on + strand, within MMJJ_RS08875 at 1636.643 kb on + strand, within MMJJ_RS08875 at 1636.648 kb on + strand, within MMJJ_RS08875 at 1636.658 kb on - strand, within MMJJ_RS08875 at 1636.659 kb on + strand, within MMJJ_RS08875 at 1636.669 kb on - strand, within MMJJ_RS08875 at 1636.669 kb on - strand, within MMJJ_RS08875 at 1636.669 kb on - strand, within MMJJ_RS08875 at 1636.669 kb on - strand, within MMJJ_RS08875 at 1636.669 kb on - strand, within MMJJ_RS08875 at 1636.669 kb on - strand, within MMJJ_RS08875 at 1636.670 kb on + strand, within MMJJ_RS08875 at 1636.670 kb on + strand, within MMJJ_RS08875 at 1636.670 kb on + strand, within MMJJ_RS08875 at 1636.718 kb on + strand, within MMJJ_RS08875 at 1636.718 kb on + strand, within MMJJ_RS08875 at 1636.719 kb on - strand, within MMJJ_RS08875 at 1636.720 kb on + strand, within MMJJ_RS08875 at 1636.720 kb on + strand, within MMJJ_RS08875 at 1636.720 kb on + strand, within MMJJ_RS08875 at 1636.748 kb on - strand, within MMJJ_RS08875 at 1636.748 kb on - strand, within MMJJ_RS08875 at 1636.748 kb on - strand, within MMJJ_RS08875 at 1636.748 kb on - strand, within MMJJ_RS08875 at 1636.748 kb on - strand, within MMJJ_RS08875 at 1636.749 kb on + strand, within MMJJ_RS08875 at 1636.749 kb on + strand, within MMJJ_RS08875 at 1636.749 kb on + strand, within MMJJ_RS08875 at 1636.753 kb on - strand, within MMJJ_RS08875 at 1636.754 kb on + strand, within MMJJ_RS08875 at 1636.761 kb on - strand, within MMJJ_RS08875 at 1636.761 kb on - strand, within MMJJ_RS08875 at 1636.762 kb on + strand, within MMJJ_RS08875 at 1636.762 kb on + strand, within MMJJ_RS08875 at 1636.794 kb on - strand, within MMJJ_RS08875 at 1636.835 kb on + strand, within MMJJ_RS08875 at 1637.008 kb on - strand, within MMJJ_RS08875 at 1637.010 kb on - strand, within MMJJ_RS08875 at 1637.010 kb on - strand, within MMJJ_RS08875 at 1637.011 kb on + strand, within MMJJ_RS08875 at 1637.036 kb on + strand, within MMJJ_RS08875 at 1637.094 kb on + strand, within MMJJ_RS08875 at 1637.103 kb on - strand, within MMJJ_RS08875 at 1637.143 kb on - strand, within MMJJ_RS08875 at 1637.144 kb on + strand, within MMJJ_RS08875 at 1637.150 kb on + strand, within MMJJ_RS08875 at 1637.208 kb on + strand, within MMJJ_RS08875 at 1637.209 kb on - strand, within MMJJ_RS08875 at 1637.363 kb on - strand, within MMJJ_RS08875 at 1637.393 kb on - strand, within MMJJ_RS08875 at 1637.396 kb on + strand, within MMJJ_RS08875 at 1637.396 kb on + strand, within MMJJ_RS08875 at 1637.406 kb on - strand, within MMJJ_RS08875 at 1637.407 kb on + strand, within MMJJ_RS08875 at 1637.407 kb on + strand, within MMJJ_RS08875 at 1637.409 kb on + strand, within MMJJ_RS08875 at 1637.451 kb on + strand, within MMJJ_RS08875 at 1637.451 kb on + strand, within MMJJ_RS08875 at 1637.459 kb on + strand, within MMJJ_RS08875 at 1637.470 kb on - strand, within MMJJ_RS08875 at 1637.470 kb on - strand, within MMJJ_RS08875 at 1637.470 kb on - strand, within MMJJ_RS08875 at 1637.471 kb on + strand, within MMJJ_RS08875 at 1637.471 kb on + strand, within MMJJ_RS08875 at 1637.478 kb on + strand, within MMJJ_RS08875 at 1637.478 kb on + strand, within MMJJ_RS08875 at 1637.481 kb on - strand, within MMJJ_RS08875 at 1637.482 kb on + strand, within MMJJ_RS08875 at 1637.482 kb on + strand, within MMJJ_RS08875 at 1637.558 kb on + strand, within MMJJ_RS08875 at 1637.584 kb on - strand, within MMJJ_RS08875 at 1637.585 kb on + strand, within MMJJ_RS08875 at 1637.607 kb on - strand, within MMJJ_RS08875 at 1637.608 kb on + strand, within MMJJ_RS08875 at 1637.612 kb on - strand, within MMJJ_RS08875 at 1637.626 kb on + strand, within MMJJ_RS08875 at 1637.662 kb on + strand, within MMJJ_RS08875 at 1637.702 kb on - strand, within MMJJ_RS08875 at 1637.781 kb on + strand, within MMJJ_RS08875 at 1637.783 kb on - strand, within MMJJ_RS08875 at 1637.785 kb on - strand, within MMJJ_RS08875 at 1637.785 kb on - strand, within MMJJ_RS08875 at 1637.789 kb on + strand, within MMJJ_RS08875 at 1637.876 kb on - strand, within MMJJ_RS08875 at 1637.895 kb on + strand, within MMJJ_RS08875 at 1637.896 kb on - strand, within MMJJ_RS08875 at 1637.896 kb on - strand, within MMJJ_RS08875 at 1637.896 kb on - strand, within MMJJ_RS08875 at 1637.897 kb on + strand, within MMJJ_RS08875 at 1637.953 kb on - strand at 1637.988 kb on + strand at 1638.018 kb on + strand at 1638.024 kb on - strand at 1638.072 kb on - strand at 1638.072 kb on - strand at 1638.072 kb on - strand at 1638.072 kb on - strand at 1638.108 kb on + strand at 1638.123 kb on - strand at 1638.123 kb on - strand at 1638.123 kb on - strand at 1638.124 kb on + strand at 1638.128 kb on - strand at 1638.159 kb on + strand at 1638.216 kb on - strand, within MMJJ_RS08880 at 1638.264 kb on - strand, within MMJJ_RS08880 at 1638.264 kb on - strand, within MMJJ_RS08880 at 1638.265 kb on + strand, within MMJJ_RS08880 at 1638.267 kb on + strand, within MMJJ_RS08880 at 1638.267 kb on + strand, within MMJJ_RS08880 at 1638.304 kb on + strand, within MMJJ_RS08880 at 1638.310 kb on + strand, within MMJJ_RS08880 at 1638.376 kb on - strand, within MMJJ_RS08880 at 1638.402 kb on - strand, within MMJJ_RS08880 at 1638.475 kb on - strand, within MMJJ_RS08880 at 1638.476 kb on + strand, within MMJJ_RS08880 at 1638.476 kb on + strand, within MMJJ_RS08880 at 1638.501 kb on - strand, within MMJJ_RS08880 at 1638.501 kb on - strand, within MMJJ_RS08880 at 1638.596 kb on - strand, within MMJJ_RS08880 at 1638.672 kb on + strand at 1638.677 kb on - strand at 1638.677 kb on - strand at 1638.677 kb on - strand at 1638.677 kb on - strand at 1638.678 kb on + strand at 1638.678 kb on + strand at 1638.702 kb on + strand at 1638.731 kb on - strand at 1638.731 kb on - strand at 1638.749 kb on - strand at 1638.753 kb on - strand at 1638.766 kb on + strand at 1638.766 kb on + strand at 1638.766 kb on + strand at 1638.830 kb on - strand at 1638.861 kb on - strand at 1638.861 kb on - strand at 1638.882 kb on - strand at 1638.883 kb on + strand at 1638.963 kb on - strand, within MMJJ_RS08885 at 1638.987 kb on - strand, within MMJJ_RS08885 at 1638.994 kb on + strand, within MMJJ_RS08885 at 1639.018 kb on + strand, within MMJJ_RS08885
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 1,636,074 - +0.6 1,636,075 + +0.0 1,636,246 - +0.3 1,636,246 - -1.7 1,636,247 + -2.7 1,636,268 - -2.0 1,636,268 - +0.4 1,636,268 - -3.0 1,636,268 - -3.3 1,636,268 - -2.7 1,636,268 - +2.0 1,636,268 - +3.7 1,636,268 - -1.8 1,636,268 - -2.9 1,636,268 - -1.4 1,636,268 - -3.1 1,636,268 - -3.3 1,636,268 - +3.3 1,636,269 + +1.9 1,636,269 + -0.4 1,636,269 + +1.0 1,636,269 + +0.6 1,636,269 + +0.7 1,636,269 + -0.2 1,636,269 + +0.4 1,636,269 + -3.0 1,636,269 + -0.8 1,636,269 + -0.3 1,636,269 + +1.5 1,636,269 + +1.1 1,636,269 + +1.2 1,636,269 + -4.4 1,636,273 + -4.8 1,636,302 - -1.3 1,636,303 + -0.2 1,636,303 + +1.1 1,636,306 - -4.1 1,636,306 - +0.3 1,636,307 + -0.6 1,636,307 + +0.3 1,636,317 - -2.4 1,636,318 + -1.0 1,636,318 + +0.8 1,636,318 + +0.6 1,636,319 - +2.1 1,636,349 - +2.0 1,636,386 - -1.4 1,636,400 - +3.3 1,636,400 - +0.7 1,636,403 + -3.3 1,636,406 - +1.3 1,636,442 - MMJJ_RS08875 0.11 -1.6 1,636,456 - MMJJ_RS08875 0.11 -1.7 1,636,469 + MMJJ_RS08875 0.12 +0.9 1,636,473 + MMJJ_RS08875 0.12 +2.5 1,636,473 + MMJJ_RS08875 0.12 -3.8 1,636,525 - MMJJ_RS08875 0.15 -0.8 1,636,525 - MMJJ_RS08875 0.15 +2.2 1,636,547 - MMJJ_RS08875 0.16 -2.7 1,636,549 - MMJJ_RS08875 0.16 +3.1 1,636,549 - MMJJ_RS08875 0.16 -1.0 1,636,549 - MMJJ_RS08875 0.16 -2.8 1,636,550 + MMJJ_RS08875 0.17 -2.4 1,636,550 + MMJJ_RS08875 0.17 +0.6 1,636,550 + MMJJ_RS08875 0.17 +1.5 1,636,572 - MMJJ_RS08875 0.18 -1.3 1,636,637 - MMJJ_RS08875 0.21 -2.8 1,636,638 + MMJJ_RS08875 0.21 -1.2 1,636,640 - MMJJ_RS08875 0.21 -0.5 1,636,640 - MMJJ_RS08875 0.21 -3.1 1,636,641 + MMJJ_RS08875 0.21 +1.2 1,636,643 + MMJJ_RS08875 0.22 +0.6 1,636,648 + MMJJ_RS08875 0.22 -0.3 1,636,658 - MMJJ_RS08875 0.22 -3.7 1,636,659 + MMJJ_RS08875 0.22 +1.8 1,636,669 - MMJJ_RS08875 0.23 +1.0 1,636,669 - MMJJ_RS08875 0.23 +0.4 1,636,669 - MMJJ_RS08875 0.23 -3.8 1,636,669 - MMJJ_RS08875 0.23 -2.0 1,636,669 - MMJJ_RS08875 0.23 -2.3 1,636,669 - MMJJ_RS08875 0.23 -0.2 1,636,670 + MMJJ_RS08875 0.23 +0.1 1,636,670 + MMJJ_RS08875 0.23 -1.0 1,636,670 + MMJJ_RS08875 0.23 +1.1 1,636,718 + MMJJ_RS08875 0.26 -2.3 1,636,718 + MMJJ_RS08875 0.26 +0.9 1,636,719 - MMJJ_RS08875 0.26 -0.7 1,636,720 + MMJJ_RS08875 0.26 +1.5 1,636,720 + MMJJ_RS08875 0.26 -1.2 1,636,720 + MMJJ_RS08875 0.26 +1.2 1,636,748 - MMJJ_RS08875 0.27 +1.9 1,636,748 - MMJJ_RS08875 0.27 +0.9 1,636,748 - MMJJ_RS08875 0.27 +2.6 1,636,748 - MMJJ_RS08875 0.27 +1.0 1,636,748 - MMJJ_RS08875 0.27 +1.6 1,636,749 + MMJJ_RS08875 0.27 +0.2 1,636,749 + MMJJ_RS08875 0.27 +2.6 1,636,749 + MMJJ_RS08875 0.27 +0.1 1,636,753 - MMJJ_RS08875 0.28 +1.2 1,636,754 + MMJJ_RS08875 0.28 -3.3 1,636,761 - MMJJ_RS08875 0.28 -2.1 1,636,761 - MMJJ_RS08875 0.28 -0.2 1,636,762 + MMJJ_RS08875 0.28 +2.9 1,636,762 + MMJJ_RS08875 0.28 -1.2 1,636,794 - MMJJ_RS08875 0.30 -2.0 1,636,835 + MMJJ_RS08875 0.32 -2.7 1,637,008 - MMJJ_RS08875 0.41 -2.7 1,637,010 - MMJJ_RS08875 0.41 -2.5 1,637,010 - MMJJ_RS08875 0.41 -0.4 1,637,011 + MMJJ_RS08875 0.42 +1.6 1,637,036 + MMJJ_RS08875 0.43 -3.3 1,637,094 + MMJJ_RS08875 0.46 -1.7 1,637,103 - MMJJ_RS08875 0.47 +0.4 1,637,143 - MMJJ_RS08875 0.49 -0.8 1,637,144 + MMJJ_RS08875 0.49 -1.6 1,637,150 + MMJJ_RS08875 0.49 +0.7 1,637,208 + MMJJ_RS08875 0.52 +2.0 1,637,209 - MMJJ_RS08875 0.52 -2.3 1,637,363 - MMJJ_RS08875 0.61 -1.2 1,637,393 - MMJJ_RS08875 0.62 -3.1 1,637,396 + MMJJ_RS08875 0.62 +3.3 1,637,396 + MMJJ_RS08875 0.62 +0.7 1,637,406 - MMJJ_RS08875 0.63 -2.6 1,637,407 + MMJJ_RS08875 0.63 -2.5 1,637,407 + MMJJ_RS08875 0.63 -3.9 1,637,409 + MMJJ_RS08875 0.63 -1.6 1,637,451 + MMJJ_RS08875 0.65 -3.0 1,637,451 + MMJJ_RS08875 0.65 -0.2 1,637,459 + MMJJ_RS08875 0.66 +0.0 1,637,470 - MMJJ_RS08875 0.66 +0.0 1,637,470 - MMJJ_RS08875 0.66 -1.3 1,637,470 - MMJJ_RS08875 0.66 -1.8 1,637,471 + MMJJ_RS08875 0.66 +1.8 1,637,471 + MMJJ_RS08875 0.66 +2.3 1,637,478 + MMJJ_RS08875 0.67 -2.6 1,637,478 + MMJJ_RS08875 0.67 -2.6 1,637,481 - MMJJ_RS08875 0.67 -0.6 1,637,482 + MMJJ_RS08875 0.67 +3.2 1,637,482 + MMJJ_RS08875 0.67 -3.5 1,637,558 + MMJJ_RS08875 0.71 +0.5 1,637,584 - MMJJ_RS08875 0.73 +3.3 1,637,585 + MMJJ_RS08875 0.73 +1.6 1,637,607 - MMJJ_RS08875 0.74 -2.3 1,637,608 + MMJJ_RS08875 0.74 -4.1 1,637,612 - MMJJ_RS08875 0.74 -2.4 1,637,626 + MMJJ_RS08875 0.75 -0.7 1,637,662 + MMJJ_RS08875 0.77 -3.5 1,637,702 - MMJJ_RS08875 0.79 -2.7 1,637,781 + MMJJ_RS08875 0.83 -2.0 1,637,783 - MMJJ_RS08875 0.83 +2.1 1,637,785 - MMJJ_RS08875 0.83 -2.9 1,637,785 - MMJJ_RS08875 0.83 +0.2 1,637,789 + MMJJ_RS08875 0.84 +1.7 1,637,876 - MMJJ_RS08875 0.88 -0.2 1,637,895 + MMJJ_RS08875 0.89 -2.0 1,637,896 - MMJJ_RS08875 0.89 -1.0 1,637,896 - MMJJ_RS08875 0.89 +1.7 1,637,896 - MMJJ_RS08875 0.89 +0.8 1,637,897 + MMJJ_RS08875 0.90 -2.1 1,637,953 - +1.2 1,637,988 + -3.3 1,638,018 + -1.2 1,638,024 - -0.5 1,638,072 - -3.7 1,638,072 - +0.7 1,638,072 - -0.7 1,638,072 - -1.2 1,638,108 + -3.7 1,638,123 - -0.8 1,638,123 - -3.7 1,638,123 - -2.6 1,638,124 + -2.1 1,638,128 - -1.2 1,638,159 + -1.7 1,638,216 - MMJJ_RS08880 0.16 -3.6 1,638,264 - MMJJ_RS08880 0.24 +2.1 1,638,264 - MMJJ_RS08880 0.24 -1.0 1,638,265 + MMJJ_RS08880 0.24 +1.8 1,638,267 + MMJJ_RS08880 0.24 +1.6 1,638,267 + MMJJ_RS08880 0.24 -1.2 1,638,304 + MMJJ_RS08880 0.30 -5.1 1,638,310 + MMJJ_RS08880 0.31 -1.2 1,638,376 - MMJJ_RS08880 0.42 +1.9 1,638,402 - MMJJ_RS08880 0.46 +1.7 1,638,475 - MMJJ_RS08880 0.58 +0.8 1,638,476 + MMJJ_RS08880 0.58 +0.8 1,638,476 + MMJJ_RS08880 0.58 +2.2 1,638,501 - MMJJ_RS08880 0.63 +1.1 1,638,501 - MMJJ_RS08880 0.63 -3.4 1,638,596 - MMJJ_RS08880 0.78 +1.7 1,638,672 + -0.3 1,638,677 - +2.3 1,638,677 - -1.8 1,638,677 - -1.7 1,638,677 - -0.4 1,638,678 + +1.2 1,638,678 + -1.4 1,638,702 + +1.0 1,638,731 - +2.1 1,638,731 - +1.0 1,638,749 - +2.6 1,638,753 - +0.9 1,638,766 + +2.6 1,638,766 + +2.6 1,638,766 + +0.7 1,638,830 - -0.1 1,638,861 - +0.1 1,638,861 - +0.9 1,638,882 - -2.0 1,638,883 + +2.5 1,638,963 - MMJJ_RS08885 0.15 +2.0 1,638,987 - MMJJ_RS08885 0.17 +0.6 1,638,994 + MMJJ_RS08885 0.17 +3.2 1,639,018 + MMJJ_RS08885 0.19 +1.8
Or see this region's nucleotide sequence