Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04955 and MMJJ_RS04960 are separated by 85 nucleotides MMJJ_RS04960 and MMJJ_RS04965 are separated by 48 nucleotides MMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotides MMJJ_RS04970 and MMJJ_RS04975 are separated by 22 nucleotides
MMJJ_RS04955: MMJJ_RS04955 - tRNA pseudouridine(54/55) synthase Pus10, at 920,964 to 922,376
_RS04955
MMJJ_RS04960: MMJJ_RS04960 - 50S ribosomal protein L21e, at 922,462 to 922,755
_RS04960
MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127
_RS04965
MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812
_RS04970
MMJJ_RS04975: MMJJ_RS04975 - glycosyltransferase family 4 protein, at 923,835 to 924,902
_RS04975
Position (kb) 922
923
924 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 921.874 kb on - strand, within MMJJ_RS04955 at 921.958 kb on - strand, within MMJJ_RS04955 at 922.026 kb on - strand, within MMJJ_RS04955 at 922.026 kb on - strand, within MMJJ_RS04955 at 922.027 kb on + strand, within MMJJ_RS04955 at 922.171 kb on - strand, within MMJJ_RS04955 at 922.172 kb on + strand, within MMJJ_RS04955 at 922.242 kb on - strand at 922.243 kb on + strand at 922.256 kb on - strand at 922.303 kb on - strand at 922.307 kb on - strand at 922.316 kb on - strand at 922.316 kb on - strand at 922.317 kb on + strand at 922.317 kb on + strand at 922.322 kb on - strand at 922.330 kb on - strand at 922.353 kb on - strand at 922.375 kb on + strand at 922.747 kb on + strand at 922.757 kb on - strand at 922.762 kb on - strand at 922.803 kb on - strand at 922.821 kb on - strand at 922.943 kb on - strand, within MMJJ_RS04965 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.947 kb on - strand, within MMJJ_RS04965 at 922.947 kb on - strand, within MMJJ_RS04965 at 922.948 kb on + strand, within MMJJ_RS04965 at 922.966 kb on + strand, within MMJJ_RS04965 at 923.061 kb on + strand, within MMJJ_RS04965 at 923.065 kb on - strand, within MMJJ_RS04965 at 923.065 kb on - strand, within MMJJ_RS04965 at 923.068 kb on + strand, within MMJJ_RS04965 at 923.108 kb on - strand at 923.108 kb on - strand at 923.109 kb on + strand at 923.116 kb on + strand at 923.144 kb on - strand at 923.145 kb on + strand at 923.145 kb on + strand at 923.147 kb on - strand at 923.187 kb on + strand at 923.190 kb on - strand at 923.190 kb on - strand at 923.191 kb on + strand at 923.196 kb on - strand at 923.318 kb on + strand, within MMJJ_RS04970 at 923.429 kb on - strand, within MMJJ_RS04970 at 923.613 kb on - strand, within MMJJ_RS04970 at 923.613 kb on - strand, within MMJJ_RS04970 at 923.860 kb on + strand at 924.025 kb on - strand, within MMJJ_RS04975 at 924.112 kb on + strand, within MMJJ_RS04975
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 921,874 - MMJJ_RS04955 0.64 -2.0 921,958 - MMJJ_RS04955 0.70 -1.3 922,026 - MMJJ_RS04955 0.75 +3.2 922,026 - MMJJ_RS04955 0.75 +0.6 922,027 + MMJJ_RS04955 0.75 -2.5 922,171 - MMJJ_RS04955 0.85 +1.0 922,172 + MMJJ_RS04955 0.85 -2.2 922,242 - -0.1 922,243 + -2.0 922,256 - -3.8 922,303 - +0.8 922,307 - +1.8 922,316 - -2.7 922,316 - -2.3 922,317 + -1.5 922,317 + +0.2 922,322 - -2.1 922,330 - -3.0 922,353 - +2.1 922,375 + +0.9 922,747 + -2.5 922,757 - -0.5 922,762 - -0.3 922,803 - +1.7 922,821 - -0.7 922,943 - MMJJ_RS04965 0.43 +1.1 922,943 - MMJJ_RS04965 0.43 -2.0 922,943 - MMJJ_RS04965 0.43 -1.6 922,947 - MMJJ_RS04965 0.44 -3.6 922,947 - MMJJ_RS04965 0.44 +0.7 922,948 + MMJJ_RS04965 0.44 -0.4 922,966 + MMJJ_RS04965 0.50 +0.4 923,061 + MMJJ_RS04965 0.79 +0.4 923,065 - MMJJ_RS04965 0.81 -0.7 923,065 - MMJJ_RS04965 0.81 -2.0 923,068 + MMJJ_RS04965 0.81 -1.1 923,108 - -1.2 923,108 - -2.3 923,109 + -1.4 923,116 + +0.6 923,144 - +3.3 923,145 + -1.7 923,145 + +1.0 923,147 - -1.2 923,187 + -0.1 923,190 - -2.0 923,190 - +0.4 923,191 + -1.3 923,196 - +1.2 923,318 + MMJJ_RS04970 0.15 -1.8 923,429 - MMJJ_RS04970 0.34 -1.4 923,613 - MMJJ_RS04970 0.66 -2.3 923,613 - MMJJ_RS04970 0.66 -0.7 923,860 + +0.4 924,025 - MMJJ_RS04975 0.18 -1.1 924,112 + MMJJ_RS04975 0.26 -1.3
Or see this region's nucleotide sequence