Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04950 and MMJJ_RS04955 are separated by 21 nucleotides MMJJ_RS04955 and MMJJ_RS04960 are separated by 85 nucleotides MMJJ_RS04960 and MMJJ_RS04965 are separated by 48 nucleotides MMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotides
MMJJ_RS04950: MMJJ_RS04950 - TIGR00375 family protein, at 919,761 to 920,942
_RS04950
MMJJ_RS04955: MMJJ_RS04955 - tRNA pseudouridine(54/55) synthase Pus10, at 920,964 to 922,376
_RS04955
MMJJ_RS04960: MMJJ_RS04960 - 50S ribosomal protein L21e, at 922,462 to 922,755
_RS04960
MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127
_RS04965
MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812
_RS04970
Position (kb) 920
921
922
923 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 919.980 kb on + strand, within MMJJ_RS04950 at 919.981 kb on - strand, within MMJJ_RS04950 at 919.982 kb on + strand, within MMJJ_RS04950 at 919.982 kb on + strand, within MMJJ_RS04950 at 920.009 kb on - strand, within MMJJ_RS04950 at 920.010 kb on + strand, within MMJJ_RS04950 at 920.010 kb on + strand, within MMJJ_RS04950 at 920.043 kb on - strand, within MMJJ_RS04950 at 920.043 kb on - strand, within MMJJ_RS04950 at 920.071 kb on - strand, within MMJJ_RS04950 at 920.097 kb on - strand, within MMJJ_RS04950 at 920.098 kb on + strand, within MMJJ_RS04950 at 920.098 kb on + strand, within MMJJ_RS04950 at 920.098 kb on + strand, within MMJJ_RS04950 at 920.130 kb on + strand, within MMJJ_RS04950 at 920.199 kb on - strand, within MMJJ_RS04950 at 920.199 kb on - strand, within MMJJ_RS04950 at 920.200 kb on + strand, within MMJJ_RS04950 at 920.200 kb on + strand, within MMJJ_RS04950 at 920.204 kb on - strand, within MMJJ_RS04950 at 920.204 kb on - strand, within MMJJ_RS04950 at 920.205 kb on + strand, within MMJJ_RS04950 at 920.205 kb on + strand, within MMJJ_RS04950 at 920.205 kb on + strand, within MMJJ_RS04950 at 920.211 kb on + strand, within MMJJ_RS04950 at 920.211 kb on + strand, within MMJJ_RS04950 at 920.215 kb on - strand, within MMJJ_RS04950 at 920.215 kb on - strand, within MMJJ_RS04950 at 920.215 kb on - strand, within MMJJ_RS04950 at 920.215 kb on - strand, within MMJJ_RS04950 at 920.215 kb on - strand, within MMJJ_RS04950 at 920.215 kb on - strand, within MMJJ_RS04950 at 920.216 kb on + strand, within MMJJ_RS04950 at 920.216 kb on + strand, within MMJJ_RS04950 at 920.216 kb on + strand, within MMJJ_RS04950 at 920.225 kb on - strand, within MMJJ_RS04950 at 920.225 kb on - strand, within MMJJ_RS04950 at 920.226 kb on + strand, within MMJJ_RS04950 at 920.226 kb on + strand, within MMJJ_RS04950 at 920.235 kb on - strand, within MMJJ_RS04950 at 920.235 kb on - strand, within MMJJ_RS04950 at 920.235 kb on - strand, within MMJJ_RS04950 at 920.235 kb on - strand, within MMJJ_RS04950 at 920.236 kb on + strand, within MMJJ_RS04950 at 920.236 kb on + strand, within MMJJ_RS04950 at 920.242 kb on - strand, within MMJJ_RS04950 at 920.243 kb on + strand, within MMJJ_RS04950 at 920.269 kb on - strand, within MMJJ_RS04950 at 920.285 kb on - strand, within MMJJ_RS04950 at 920.286 kb on + strand, within MMJJ_RS04950 at 920.286 kb on + strand, within MMJJ_RS04950 at 920.307 kb on - strand, within MMJJ_RS04950 at 920.308 kb on + strand, within MMJJ_RS04950 at 920.308 kb on + strand, within MMJJ_RS04950 at 920.346 kb on - strand, within MMJJ_RS04950 at 920.346 kb on - strand, within MMJJ_RS04950 at 920.346 kb on - strand, within MMJJ_RS04950 at 920.346 kb on - strand, within MMJJ_RS04950 at 920.346 kb on - strand, within MMJJ_RS04950 at 920.347 kb on + strand, within MMJJ_RS04950 at 920.347 kb on + strand, within MMJJ_RS04950 at 920.347 kb on + strand, within MMJJ_RS04950 at 920.347 kb on + strand, within MMJJ_RS04950 at 920.512 kb on - strand, within MMJJ_RS04950 at 920.522 kb on - strand, within MMJJ_RS04950 at 920.533 kb on - strand, within MMJJ_RS04950 at 920.533 kb on - strand, within MMJJ_RS04950 at 920.538 kb on + strand, within MMJJ_RS04950 at 920.703 kb on + strand, within MMJJ_RS04950 at 920.713 kb on - strand, within MMJJ_RS04950 at 920.713 kb on - strand, within MMJJ_RS04950 at 920.714 kb on + strand, within MMJJ_RS04950 at 920.716 kb on + strand, within MMJJ_RS04950 at 920.718 kb on - strand, within MMJJ_RS04950 at 920.719 kb on + strand, within MMJJ_RS04950 at 920.832 kb on - strand at 920.847 kb on - strand at 920.857 kb on - strand at 920.857 kb on - strand at 920.857 kb on - strand at 920.863 kb on + strand at 920.865 kb on - strand at 920.865 kb on - strand at 920.865 kb on - strand at 920.865 kb on - strand at 920.865 kb on - strand at 920.865 kb on - strand at 920.866 kb on + strand at 920.866 kb on + strand at 920.866 kb on + strand at 920.866 kb on + strand at 920.867 kb on - strand at 920.867 kb on - strand at 920.867 kb on - strand at 920.867 kb on - strand at 920.867 kb on - strand at 920.867 kb on - strand at 920.867 kb on - strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.868 kb on + strand at 920.882 kb on - strand at 920.882 kb on - strand at 920.882 kb on - strand at 920.882 kb on - strand at 920.883 kb on + strand at 920.884 kb on - strand at 920.884 kb on - strand at 920.884 kb on - strand at 920.884 kb on - strand at 920.884 kb on - strand at 920.884 kb on - strand at 920.884 kb on - strand at 920.885 kb on + strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.888 kb on - strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.889 kb on + strand at 920.900 kb on - strand at 920.900 kb on - strand at 920.900 kb on - strand at 920.900 kb on - strand at 920.901 kb on + strand at 920.918 kb on - strand at 920.919 kb on + strand at 920.922 kb on - strand at 920.922 kb on - strand at 920.922 kb on - strand at 920.923 kb on + strand at 920.923 kb on + strand at 920.923 kb on + strand at 920.923 kb on + strand at 920.923 kb on + strand at 920.923 kb on + strand at 920.934 kb on - strand at 920.934 kb on - strand at 920.934 kb on - strand at 920.934 kb on - strand at 920.935 kb on + strand at 920.935 kb on + strand at 920.935 kb on + strand at 920.962 kb on + strand at 920.963 kb on - strand at 920.963 kb on - strand at 920.964 kb on + strand at 920.964 kb on + strand at 920.964 kb on + strand at 920.970 kb on + strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.971 kb on - strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.972 kb on + strand at 920.976 kb on - strand at 920.976 kb on - strand at 921.038 kb on - strand at 921.041 kb on - strand at 921.041 kb on - strand at 921.110 kb on + strand, within MMJJ_RS04955 at 921.166 kb on - strand, within MMJJ_RS04955 at 921.205 kb on + strand, within MMJJ_RS04955 at 921.205 kb on + strand, within MMJJ_RS04955 at 921.247 kb on - strand, within MMJJ_RS04955 at 921.385 kb on - strand, within MMJJ_RS04955 at 921.386 kb on + strand, within MMJJ_RS04955 at 921.390 kb on + strand, within MMJJ_RS04955 at 921.390 kb on + strand, within MMJJ_RS04955 at 921.583 kb on - strand, within MMJJ_RS04955 at 921.583 kb on - strand, within MMJJ_RS04955 at 921.584 kb on + strand, within MMJJ_RS04955 at 921.597 kb on - strand, within MMJJ_RS04955 at 921.597 kb on - strand, within MMJJ_RS04955 at 921.598 kb on + strand, within MMJJ_RS04955 at 921.692 kb on - strand, within MMJJ_RS04955 at 921.693 kb on + strand, within MMJJ_RS04955 at 921.731 kb on - strand, within MMJJ_RS04955 at 921.744 kb on - strand, within MMJJ_RS04955 at 921.745 kb on + strand, within MMJJ_RS04955 at 921.745 kb on + strand, within MMJJ_RS04955 at 921.767 kb on - strand, within MMJJ_RS04955 at 921.874 kb on - strand, within MMJJ_RS04955 at 921.958 kb on - strand, within MMJJ_RS04955 at 922.026 kb on - strand, within MMJJ_RS04955 at 922.026 kb on - strand, within MMJJ_RS04955 at 922.027 kb on + strand, within MMJJ_RS04955 at 922.171 kb on - strand, within MMJJ_RS04955 at 922.172 kb on + strand, within MMJJ_RS04955 at 922.242 kb on - strand at 922.243 kb on + strand at 922.256 kb on - strand at 922.303 kb on - strand at 922.307 kb on - strand at 922.316 kb on - strand at 922.316 kb on - strand at 922.317 kb on + strand at 922.317 kb on + strand at 922.322 kb on - strand at 922.330 kb on - strand at 922.353 kb on - strand at 922.375 kb on + strand at 922.747 kb on + strand at 922.757 kb on - strand at 922.762 kb on - strand at 922.803 kb on - strand at 922.821 kb on - strand at 922.943 kb on - strand, within MMJJ_RS04965 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.943 kb on - strand, within MMJJ_RS04965 at 922.947 kb on - strand, within MMJJ_RS04965 at 922.947 kb on - strand, within MMJJ_RS04965 at 922.948 kb on + strand, within MMJJ_RS04965 at 922.966 kb on + strand, within MMJJ_RS04965 at 923.061 kb on + strand, within MMJJ_RS04965 at 923.065 kb on - strand, within MMJJ_RS04965 at 923.065 kb on - strand, within MMJJ_RS04965 at 923.068 kb on + strand, within MMJJ_RS04965 at 923.108 kb on - strand at 923.108 kb on - strand at 923.109 kb on + strand at 923.116 kb on + strand at 923.144 kb on - strand at 923.145 kb on + strand at 923.145 kb on + strand at 923.147 kb on - strand at 923.187 kb on + strand at 923.190 kb on - strand at 923.190 kb on - strand at 923.191 kb on + strand at 923.196 kb on - strand at 923.318 kb on + strand, within MMJJ_RS04970
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 919,980 + MMJJ_RS04950 0.19 -2.4 919,981 - MMJJ_RS04950 0.19 -4.0 919,982 + MMJJ_RS04950 0.19 -1.3 919,982 + MMJJ_RS04950 0.19 -1.9 920,009 - MMJJ_RS04950 0.21 -0.3 920,010 + MMJJ_RS04950 0.21 -2.2 920,010 + MMJJ_RS04950 0.21 -1.4 920,043 - MMJJ_RS04950 0.24 -1.5 920,043 - MMJJ_RS04950 0.24 -0.3 920,071 - MMJJ_RS04950 0.26 +0.1 920,097 - MMJJ_RS04950 0.28 -1.4 920,098 + MMJJ_RS04950 0.29 +0.2 920,098 + MMJJ_RS04950 0.29 -2.1 920,098 + MMJJ_RS04950 0.29 -0.1 920,130 + MMJJ_RS04950 0.31 -0.3 920,199 - MMJJ_RS04950 0.37 +1.2 920,199 - MMJJ_RS04950 0.37 -0.1 920,200 + MMJJ_RS04950 0.37 +2.7 920,200 + MMJJ_RS04950 0.37 -3.8 920,204 - MMJJ_RS04950 0.37 -3.2 920,204 - MMJJ_RS04950 0.37 -1.0 920,205 + MMJJ_RS04950 0.38 +1.9 920,205 + MMJJ_RS04950 0.38 +1.1 920,205 + MMJJ_RS04950 0.38 -2.2 920,211 + MMJJ_RS04950 0.38 +2.9 920,211 + MMJJ_RS04950 0.38 +1.4 920,215 - MMJJ_RS04950 0.38 +0.1 920,215 - MMJJ_RS04950 0.38 +0.0 920,215 - MMJJ_RS04950 0.38 +1.5 920,215 - MMJJ_RS04950 0.38 -3.1 920,215 - MMJJ_RS04950 0.38 -2.8 920,215 - MMJJ_RS04950 0.38 -4.2 920,216 + MMJJ_RS04950 0.38 +1.2 920,216 + MMJJ_RS04950 0.38 +6.7 920,216 + MMJJ_RS04950 0.38 -0.6 920,225 - MMJJ_RS04950 0.39 -1.5 920,225 - MMJJ_RS04950 0.39 +2.7 920,226 + MMJJ_RS04950 0.39 -2.1 920,226 + MMJJ_RS04950 0.39 +2.7 920,235 - MMJJ_RS04950 0.40 -2.0 920,235 - MMJJ_RS04950 0.40 +1.3 920,235 - MMJJ_RS04950 0.40 +0.9 920,235 - MMJJ_RS04950 0.40 +3.6 920,236 + MMJJ_RS04950 0.40 -0.1 920,236 + MMJJ_RS04950 0.40 +0.2 920,242 - MMJJ_RS04950 0.41 -0.8 920,243 + MMJJ_RS04950 0.41 -1.3 920,269 - MMJJ_RS04950 0.43 +2.3 920,285 - MMJJ_RS04950 0.44 +2.3 920,286 + MMJJ_RS04950 0.44 -0.6 920,286 + MMJJ_RS04950 0.44 -1.9 920,307 - MMJJ_RS04950 0.46 -0.2 920,308 + MMJJ_RS04950 0.46 -2.8 920,308 + MMJJ_RS04950 0.46 -3.3 920,346 - MMJJ_RS04950 0.49 +4.1 920,346 - MMJJ_RS04950 0.49 +1.7 920,346 - MMJJ_RS04950 0.49 -0.1 920,346 - MMJJ_RS04950 0.49 +1.8 920,346 - MMJJ_RS04950 0.49 -0.5 920,347 + MMJJ_RS04950 0.50 -1.4 920,347 + MMJJ_RS04950 0.50 -1.4 920,347 + MMJJ_RS04950 0.50 +3.5 920,347 + MMJJ_RS04950 0.50 +1.9 920,512 - MMJJ_RS04950 0.64 +3.4 920,522 - MMJJ_RS04950 0.64 +0.5 920,533 - MMJJ_RS04950 0.65 +1.4 920,533 - MMJJ_RS04950 0.65 -1.5 920,538 + MMJJ_RS04950 0.66 -1.7 920,703 + MMJJ_RS04950 0.80 +2.4 920,713 - MMJJ_RS04950 0.81 -2.5 920,713 - MMJJ_RS04950 0.81 -0.5 920,714 + MMJJ_RS04950 0.81 +1.9 920,716 + MMJJ_RS04950 0.81 -0.6 920,718 - MMJJ_RS04950 0.81 -1.2 920,719 + MMJJ_RS04950 0.81 +0.4 920,832 - +0.9 920,847 - +0.8 920,857 - -2.4 920,857 - +0.1 920,857 - -0.3 920,863 + -0.8 920,865 - -1.8 920,865 - -2.7 920,865 - +3.3 920,865 - -2.8 920,865 - -0.9 920,865 - -1.0 920,866 + +1.5 920,866 + -0.9 920,866 + +1.6 920,866 + -1.6 920,867 - -1.3 920,867 - -4.1 920,867 - -0.5 920,867 - -2.3 920,867 - +3.1 920,867 - -1.0 920,867 - +1.9 920,868 + +0.9 920,868 + +0.8 920,868 + +0.6 920,868 + -3.2 920,868 + +0.0 920,868 + -2.2 920,868 + +1.0 920,868 + -0.0 920,868 + +2.7 920,868 + -2.4 920,868 + -1.0 920,882 - +1.4 920,882 - +0.5 920,882 - -0.1 920,882 - -0.2 920,883 + -1.8 920,884 - -1.8 920,884 - -0.7 920,884 - +1.8 920,884 - +1.5 920,884 - +3.8 920,884 - -2.2 920,884 - +0.7 920,885 + +2.8 920,888 - +0.2 920,888 - -0.4 920,888 - -2.7 920,888 - +3.9 920,888 - -4.0 920,888 - +0.3 920,888 - +1.2 920,888 - -1.6 920,888 - -1.7 920,888 - -3.1 920,888 - -1.3 920,889 + +1.2 920,889 + -0.6 920,889 + -4.9 920,889 + +1.7 920,889 + -1.2 920,889 + +0.4 920,889 + +3.1 920,889 + +2.2 920,889 + -1.3 920,889 + +1.0 920,900 - +1.5 920,900 - -0.8 920,900 - +0.4 920,900 - -2.8 920,901 + +0.4 920,918 - +3.7 920,919 + +0.8 920,922 - +0.7 920,922 - +0.2 920,922 - +1.4 920,923 + +0.1 920,923 + +0.0 920,923 + +1.3 920,923 + -1.6 920,923 + +1.2 920,923 + -2.5 920,934 - -1.2 920,934 - -0.8 920,934 - -0.8 920,934 - +0.2 920,935 + -2.8 920,935 + +2.6 920,935 + -0.8 920,962 + -2.2 920,963 - +3.0 920,963 - -3.2 920,964 + +1.2 920,964 + -1.6 920,964 + -1.1 920,970 + +1.9 920,971 - +3.2 920,971 - -0.8 920,971 - +2.5 920,971 - -2.6 920,971 - +0.3 920,971 - +0.9 920,971 - +1.7 920,971 - +0.8 920,971 - -2.3 920,971 - -1.8 920,972 + -3.2 920,972 + -2.7 920,972 + -1.8 920,972 + -0.2 920,972 + +0.3 920,972 + +3.0 920,972 + -0.8 920,972 + -1.6 920,972 + -1.0 920,972 + +3.7 920,976 - +4.2 920,976 - +0.7 921,038 - -1.2 921,041 - +2.6 921,041 - +1.6 921,110 + MMJJ_RS04955 0.10 +0.4 921,166 - MMJJ_RS04955 0.14 +1.8 921,205 + MMJJ_RS04955 0.17 +3.3 921,205 + MMJJ_RS04955 0.17 +0.9 921,247 - MMJJ_RS04955 0.20 -0.7 921,385 - MMJJ_RS04955 0.30 -0.5 921,386 + MMJJ_RS04955 0.30 +4.1 921,390 + MMJJ_RS04955 0.30 -3.7 921,390 + MMJJ_RS04955 0.30 -2.7 921,583 - MMJJ_RS04955 0.44 +3.5 921,583 - MMJJ_RS04955 0.44 -2.3 921,584 + MMJJ_RS04955 0.44 -0.6 921,597 - MMJJ_RS04955 0.45 -2.0 921,597 - MMJJ_RS04955 0.45 +1.6 921,598 + MMJJ_RS04955 0.45 +1.9 921,692 - MMJJ_RS04955 0.52 +2.8 921,693 + MMJJ_RS04955 0.52 -2.0 921,731 - MMJJ_RS04955 0.54 +0.4 921,744 - MMJJ_RS04955 0.55 -0.6 921,745 + MMJJ_RS04955 0.55 -0.9 921,745 + MMJJ_RS04955 0.55 +2.8 921,767 - MMJJ_RS04955 0.57 -4.3 921,874 - MMJJ_RS04955 0.64 -2.0 921,958 - MMJJ_RS04955 0.70 -1.3 922,026 - MMJJ_RS04955 0.75 +3.2 922,026 - MMJJ_RS04955 0.75 +0.6 922,027 + MMJJ_RS04955 0.75 -2.5 922,171 - MMJJ_RS04955 0.85 +1.0 922,172 + MMJJ_RS04955 0.85 -2.2 922,242 - -0.1 922,243 + -2.0 922,256 - -3.8 922,303 - +0.8 922,307 - +1.8 922,316 - -2.7 922,316 - -2.3 922,317 + -1.5 922,317 + +0.2 922,322 - -2.1 922,330 - -3.0 922,353 - +2.1 922,375 + +0.9 922,747 + -2.5 922,757 - -0.5 922,762 - -0.3 922,803 - +1.7 922,821 - -0.7 922,943 - MMJJ_RS04965 0.43 +1.1 922,943 - MMJJ_RS04965 0.43 -2.0 922,943 - MMJJ_RS04965 0.43 -1.6 922,947 - MMJJ_RS04965 0.44 -3.6 922,947 - MMJJ_RS04965 0.44 +0.7 922,948 + MMJJ_RS04965 0.44 -0.4 922,966 + MMJJ_RS04965 0.50 +0.4 923,061 + MMJJ_RS04965 0.79 +0.4 923,065 - MMJJ_RS04965 0.81 -0.7 923,065 - MMJJ_RS04965 0.81 -2.0 923,068 + MMJJ_RS04965 0.81 -1.1 923,108 - -1.2 923,108 - -2.3 923,109 + -1.4 923,116 + +0.6 923,144 - +3.3 923,145 + -1.7 923,145 + +1.0 923,147 - -1.2 923,187 + -0.1 923,190 - -2.0 923,190 - +0.4 923,191 + -1.3 923,196 - +1.2 923,318 + MMJJ_RS04970 0.15 -1.8
Or see this region's nucleotide sequence