Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04530 and MMJJ_RS04535 are separated by 3 nucleotides MMJJ_RS04535 and MMJJ_RS04540 are separated by 21 nucleotides MMJJ_RS04540 and MMJJ_RS04545 are separated by 112 nucleotides MMJJ_RS04545 and MMJJ_RS04550 are separated by 1 nucleotides
MMJJ_RS04530: MMJJ_RS04530 - iron export ABC transporter permease subunit FetB, at 845,493 to 846,239
_RS04530
MMJJ_RS04535: MMJJ_RS04535 - coenzyme gamma-F420-2:alpha-L-glutamate ligase, at 846,243 to 847,100
_RS04535
MMJJ_RS04540: MMJJ_RS04540 - site-2 protease family protein, at 847,122 to 848,249
_RS04540
MMJJ_RS04545: MMJJ_RS04545 - PadR family transcriptional regulator, at 848,362 to 848,820
_RS04545
MMJJ_RS04550: MMJJ_RS04550 - ATP-binding cassette domain-containing protein, at 848,822 to 849,790
_RS04550
Position (kb) 847
848
849 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 846.141 kb on - strand, within MMJJ_RS04530 at 846.142 kb on + strand, within MMJJ_RS04530 at 846.180 kb on - strand at 846.180 kb on - strand at 846.262 kb on + strand at 846.349 kb on - strand, within MMJJ_RS04535 at 846.349 kb on - strand, within MMJJ_RS04535 at 846.350 kb on + strand, within MMJJ_RS04535 at 846.350 kb on + strand, within MMJJ_RS04535 at 846.350 kb on + strand, within MMJJ_RS04535 at 846.352 kb on - strand, within MMJJ_RS04535 at 846.352 kb on - strand, within MMJJ_RS04535 at 846.353 kb on + strand, within MMJJ_RS04535 at 846.389 kb on - strand, within MMJJ_RS04535 at 846.389 kb on - strand, within MMJJ_RS04535 at 846.389 kb on - strand, within MMJJ_RS04535 at 846.390 kb on + strand, within MMJJ_RS04535 at 846.390 kb on + strand, within MMJJ_RS04535 at 846.390 kb on + strand, within MMJJ_RS04535 at 846.444 kb on - strand, within MMJJ_RS04535 at 846.445 kb on + strand, within MMJJ_RS04535 at 846.475 kb on - strand, within MMJJ_RS04535 at 846.475 kb on - strand, within MMJJ_RS04535 at 846.475 kb on - strand, within MMJJ_RS04535 at 846.476 kb on + strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.477 kb on - strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.478 kb on + strand, within MMJJ_RS04535 at 846.494 kb on - strand, within MMJJ_RS04535 at 846.497 kb on - strand, within MMJJ_RS04535 at 846.551 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.559 kb on - strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.560 kb on + strand, within MMJJ_RS04535 at 846.587 kb on + strand, within MMJJ_RS04535 at 846.637 kb on + strand, within MMJJ_RS04535 at 846.699 kb on - strand, within MMJJ_RS04535 at 846.714 kb on - strand, within MMJJ_RS04535 at 846.834 kb on + strand, within MMJJ_RS04535 at 846.888 kb on - strand, within MMJJ_RS04535 at 846.918 kb on + strand, within MMJJ_RS04535 at 846.952 kb on + strand, within MMJJ_RS04535 at 846.952 kb on + strand, within MMJJ_RS04535 at 846.955 kb on + strand, within MMJJ_RS04535 at 846.967 kb on + strand, within MMJJ_RS04535 at 847.017 kb on + strand at 847.020 kb on + strand at 847.060 kb on - strand at 847.060 kb on - strand at 847.060 kb on - strand at 847.061 kb on + strand at 847.062 kb on - strand at 847.063 kb on + strand at 847.063 kb on + strand at 847.096 kb on + strand at 847.136 kb on + strand at 847.149 kb on - strand at 847.150 kb on + strand at 847.152 kb on - strand at 847.153 kb on + strand at 847.164 kb on - strand at 847.164 kb on - strand at 847.170 kb on - strand at 847.170 kb on - strand at 847.171 kb on + strand at 847.171 kb on + strand at 847.171 kb on + strand at 847.172 kb on - strand at 847.172 kb on - strand at 847.172 kb on - strand at 847.172 kb on - strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.173 kb on + strand at 847.199 kb on - strand at 847.203 kb on - strand at 847.321 kb on + strand, within MMJJ_RS04540 at 847.321 kb on + strand, within MMJJ_RS04540 at 847.348 kb on + strand, within MMJJ_RS04540 at 847.350 kb on - strand, within MMJJ_RS04540 at 847.363 kb on - strand, within MMJJ_RS04540 at 847.374 kb on - strand, within MMJJ_RS04540 at 847.375 kb on + strand, within MMJJ_RS04540 at 847.379 kb on - strand, within MMJJ_RS04540 at 847.379 kb on - strand, within MMJJ_RS04540 at 847.381 kb on - strand, within MMJJ_RS04540 at 847.382 kb on + strand, within MMJJ_RS04540 at 847.382 kb on + strand, within MMJJ_RS04540 at 847.382 kb on + strand, within MMJJ_RS04540 at 847.441 kb on + strand, within MMJJ_RS04540 at 847.441 kb on + strand, within MMJJ_RS04540 at 847.444 kb on - strand, within MMJJ_RS04540 at 847.444 kb on - strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.445 kb on + strand, within MMJJ_RS04540 at 847.463 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.503 kb on - strand, within MMJJ_RS04540 at 847.504 kb on + strand at 847.504 kb on + strand, within MMJJ_RS04540 at 847.565 kb on + strand, within MMJJ_RS04540 at 847.569 kb on - strand, within MMJJ_RS04540 at 847.570 kb on + strand, within MMJJ_RS04540 at 847.604 kb on - strand, within MMJJ_RS04540 at 847.655 kb on + strand, within MMJJ_RS04540 at 847.671 kb on - strand at 847.690 kb on + strand, within MMJJ_RS04540 at 847.690 kb on + strand, within MMJJ_RS04540 at 847.767 kb on - strand, within MMJJ_RS04540 at 847.872 kb on + strand, within MMJJ_RS04540 at 847.884 kb on - strand, within MMJJ_RS04540 at 847.885 kb on + strand, within MMJJ_RS04540 at 847.885 kb on + strand, within MMJJ_RS04540 at 847.916 kb on - strand, within MMJJ_RS04540 at 847.917 kb on + strand, within MMJJ_RS04540 at 847.994 kb on - strand, within MMJJ_RS04540 at 847.995 kb on + strand, within MMJJ_RS04540 at 848.064 kb on - strand, within MMJJ_RS04540 at 848.065 kb on + strand, within MMJJ_RS04540 at 848.066 kb on - strand, within MMJJ_RS04540 at 848.067 kb on + strand, within MMJJ_RS04540 at 848.067 kb on + strand, within MMJJ_RS04540 at 848.089 kb on + strand, within MMJJ_RS04540 at 848.092 kb on + strand, within MMJJ_RS04540 at 848.137 kb on - strand at 848.185 kb on + strand at 848.192 kb on + strand at 848.211 kb on - strand at 848.241 kb on + strand at 848.267 kb on - strand at 848.267 kb on - strand at 848.267 kb on - strand at 848.272 kb on + strand at 848.272 kb on + strand at 848.282 kb on - strand at 848.282 kb on - strand at 848.304 kb on + strand at 848.308 kb on + strand at 848.316 kb on + strand at 848.346 kb on - strand at 848.360 kb on - strand at 848.374 kb on + strand at 848.374 kb on + strand at 848.382 kb on + strand at 848.405 kb on - strand at 848.606 kb on - strand, within MMJJ_RS04545 at 848.623 kb on + strand, within MMJJ_RS04545 at 848.662 kb on - strand, within MMJJ_RS04545 at 848.708 kb on - strand, within MMJJ_RS04545 at 848.819 kb on + strand at 848.882 kb on + strand at 848.893 kb on - strand at 848.958 kb on - strand, within MMJJ_RS04550 at 848.962 kb on - strand, within MMJJ_RS04550 at 848.964 kb on - strand, within MMJJ_RS04550 at 848.964 kb on - strand, within MMJJ_RS04550 at 848.965 kb on + strand, within MMJJ_RS04550 at 848.965 kb on + strand, within MMJJ_RS04550 at 848.968 kb on - strand, within MMJJ_RS04550 at 848.968 kb on - strand, within MMJJ_RS04550 at 848.969 kb on + strand, within MMJJ_RS04550 at 848.995 kb on - strand, within MMJJ_RS04550 at 849.026 kb on + strand, within MMJJ_RS04550 at 849.076 kb on - strand, within MMJJ_RS04550 at 849.131 kb on - strand, within MMJJ_RS04550 at 849.137 kb on - strand, within MMJJ_RS04550 at 849.138 kb on + strand, within MMJJ_RS04550 at 849.138 kb on + strand, within MMJJ_RS04550 at 849.187 kb on + strand, within MMJJ_RS04550 at 849.203 kb on + strand, within MMJJ_RS04550 at 849.209 kb on + strand, within MMJJ_RS04550 at 849.209 kb on + strand, within MMJJ_RS04550
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 846,141 - MMJJ_RS04530 0.87 +3.1 846,142 + MMJJ_RS04530 0.87 +0.4 846,180 - +0.4 846,180 - -2.4 846,262 + +0.2 846,349 - MMJJ_RS04535 0.12 -0.6 846,349 - MMJJ_RS04535 0.12 +0.6 846,350 + MMJJ_RS04535 0.12 +0.2 846,350 + MMJJ_RS04535 0.12 +3.6 846,350 + MMJJ_RS04535 0.12 +1.9 846,352 - MMJJ_RS04535 0.13 -1.0 846,352 - MMJJ_RS04535 0.13 -0.7 846,353 + MMJJ_RS04535 0.13 +1.3 846,389 - MMJJ_RS04535 0.17 +1.5 846,389 - MMJJ_RS04535 0.17 +6.9 846,389 - MMJJ_RS04535 0.17 -2.0 846,390 + MMJJ_RS04535 0.17 -1.2 846,390 + MMJJ_RS04535 0.17 +1.6 846,390 + MMJJ_RS04535 0.17 +0.7 846,444 - MMJJ_RS04535 0.23 +0.2 846,445 + MMJJ_RS04535 0.24 +1.8 846,475 - MMJJ_RS04535 0.27 -4.7 846,475 - MMJJ_RS04535 0.27 +0.9 846,475 - MMJJ_RS04535 0.27 -1.6 846,476 + MMJJ_RS04535 0.27 -2.0 846,477 - MMJJ_RS04535 0.27 +0.3 846,477 - MMJJ_RS04535 0.27 +2.0 846,477 - MMJJ_RS04535 0.27 +0.9 846,477 - MMJJ_RS04535 0.27 +2.1 846,477 - MMJJ_RS04535 0.27 -2.3 846,478 + MMJJ_RS04535 0.27 +3.4 846,478 + MMJJ_RS04535 0.27 -1.9 846,478 + MMJJ_RS04535 0.27 -0.6 846,478 + MMJJ_RS04535 0.27 -1.1 846,494 - MMJJ_RS04535 0.29 -2.1 846,497 - MMJJ_RS04535 0.30 -1.1 846,551 - MMJJ_RS04535 0.36 -3.3 846,559 - MMJJ_RS04535 0.37 -3.1 846,559 - MMJJ_RS04535 0.37 +0.2 846,559 - MMJJ_RS04535 0.37 -0.5 846,559 - MMJJ_RS04535 0.37 -0.3 846,559 - MMJJ_RS04535 0.37 -0.3 846,560 + MMJJ_RS04535 0.37 +1.5 846,560 + MMJJ_RS04535 0.37 +1.2 846,560 + MMJJ_RS04535 0.37 -0.5 846,560 + MMJJ_RS04535 0.37 -0.8 846,560 + MMJJ_RS04535 0.37 +4.2 846,587 + MMJJ_RS04535 0.40 +1.6 846,637 + MMJJ_RS04535 0.46 +1.3 846,699 - MMJJ_RS04535 0.53 -0.6 846,714 - MMJJ_RS04535 0.55 -1.7 846,834 + MMJJ_RS04535 0.69 +1.6 846,888 - MMJJ_RS04535 0.75 -0.2 846,918 + MMJJ_RS04535 0.79 -2.6 846,952 + MMJJ_RS04535 0.83 -1.6 846,952 + MMJJ_RS04535 0.83 -1.6 846,955 + MMJJ_RS04535 0.83 +0.8 846,967 + MMJJ_RS04535 0.84 +1.1 847,017 + -3.5 847,020 + +0.8 847,060 - +1.8 847,060 - +1.6 847,060 - +1.3 847,061 + +3.8 847,062 - +2.0 847,063 + -3.7 847,063 + +1.5 847,096 + -4.1 847,136 + -2.6 847,149 - -0.4 847,150 + -3.0 847,152 - +2.5 847,153 + -2.2 847,164 - -3.1 847,164 - +4.8 847,170 - +0.1 847,170 - +0.9 847,171 + -0.5 847,171 + +0.2 847,171 + -2.7 847,172 - +6.4 847,172 - -0.5 847,172 - -1.5 847,172 - -0.1 847,173 + -3.4 847,173 + +0.3 847,173 + +1.7 847,173 + -1.6 847,173 + -2.0 847,199 - -1.6 847,203 - +3.2 847,321 + MMJJ_RS04540 0.18 -1.4 847,321 + MMJJ_RS04540 0.18 -0.8 847,348 + MMJJ_RS04540 0.20 +5.2 847,350 - MMJJ_RS04540 0.20 -1.5 847,363 - MMJJ_RS04540 0.21 +2.2 847,374 - MMJJ_RS04540 0.22 -0.9 847,375 + MMJJ_RS04540 0.22 -0.1 847,379 - MMJJ_RS04540 0.23 +0.8 847,379 - MMJJ_RS04540 0.23 -0.8 847,381 - MMJJ_RS04540 0.23 +2.3 847,382 + MMJJ_RS04540 0.23 -0.5 847,382 + MMJJ_RS04540 0.23 +0.0 847,382 + MMJJ_RS04540 0.23 -2.0 847,441 + MMJJ_RS04540 0.28 -0.7 847,441 + MMJJ_RS04540 0.28 -0.7 847,444 - MMJJ_RS04540 0.29 -1.2 847,444 - MMJJ_RS04540 0.29 +0.4 847,445 + MMJJ_RS04540 0.29 +0.2 847,445 + MMJJ_RS04540 0.29 +1.3 847,445 + MMJJ_RS04540 0.29 +0.9 847,445 + MMJJ_RS04540 0.29 -1.5 847,445 + MMJJ_RS04540 0.29 +2.8 847,463 - MMJJ_RS04540 0.30 +3.6 847,503 - MMJJ_RS04540 0.34 -3.2 847,503 - MMJJ_RS04540 0.34 +0.3 847,503 - MMJJ_RS04540 0.34 -2.2 847,503 - MMJJ_RS04540 0.34 -2.3 847,503 - MMJJ_RS04540 0.34 +4.2 847,504 + +2.8 847,504 + MMJJ_RS04540 0.34 -3.6 847,565 + MMJJ_RS04540 0.39 +1.8 847,569 - MMJJ_RS04540 0.40 +2.1 847,570 + MMJJ_RS04540 0.40 +2.4 847,604 - MMJJ_RS04540 0.43 +1.2 847,655 + MMJJ_RS04540 0.47 +0.2 847,671 - +1.8 847,690 + MMJJ_RS04540 0.50 -2.3 847,690 + MMJJ_RS04540 0.50 -0.4 847,767 - MMJJ_RS04540 0.57 -1.3 847,872 + MMJJ_RS04540 0.66 +0.3 847,884 - MMJJ_RS04540 0.68 +2.8 847,885 + MMJJ_RS04540 0.68 +1.3 847,885 + MMJJ_RS04540 0.68 +2.2 847,916 - MMJJ_RS04540 0.70 +2.0 847,917 + MMJJ_RS04540 0.70 -1.8 847,994 - MMJJ_RS04540 0.77 +1.5 847,995 + MMJJ_RS04540 0.77 -0.1 848,064 - MMJJ_RS04540 0.84 -0.8 848,065 + MMJJ_RS04540 0.84 -3.2 848,066 - MMJJ_RS04540 0.84 +0.4 848,067 + MMJJ_RS04540 0.84 +0.3 848,067 + MMJJ_RS04540 0.84 +3.2 848,089 + MMJJ_RS04540 0.86 +0.8 848,092 + MMJJ_RS04540 0.86 +0.1 848,137 - -2.9 848,185 + +0.1 848,192 + -1.0 848,211 - +0.7 848,241 + +0.8 848,267 - +0.7 848,267 - +2.5 848,267 - -2.1 848,272 + +0.4 848,272 + +0.7 848,282 - +0.1 848,282 - -0.1 848,304 + +2.2 848,308 + -1.6 848,316 + -1.0 848,346 - +0.4 848,360 - -1.6 848,374 + -3.9 848,374 + +2.3 848,382 + +0.1 848,405 - +2.4 848,606 - MMJJ_RS04545 0.53 -0.3 848,623 + MMJJ_RS04545 0.57 +1.7 848,662 - MMJJ_RS04545 0.65 -3.3 848,708 - MMJJ_RS04545 0.75 +3.0 848,819 + -1.8 848,882 + +0.4 848,893 - -0.0 848,958 - MMJJ_RS04550 0.14 -0.9 848,962 - MMJJ_RS04550 0.14 +1.4 848,964 - MMJJ_RS04550 0.15 -4.1 848,964 - MMJJ_RS04550 0.15 -2.7 848,965 + MMJJ_RS04550 0.15 -0.8 848,965 + MMJJ_RS04550 0.15 -0.1 848,968 - MMJJ_RS04550 0.15 -0.0 848,968 - MMJJ_RS04550 0.15 -0.1 848,969 + MMJJ_RS04550 0.15 +2.5 848,995 - MMJJ_RS04550 0.18 +0.9 849,026 + MMJJ_RS04550 0.21 +1.9 849,076 - MMJJ_RS04550 0.26 -3.5 849,131 - MMJJ_RS04550 0.32 -1.4 849,137 - MMJJ_RS04550 0.33 +3.8 849,138 + MMJJ_RS04550 0.33 +1.1 849,138 + MMJJ_RS04550 0.33 -3.4 849,187 + MMJJ_RS04550 0.38 -2.0 849,203 + MMJJ_RS04550 0.39 -3.4 849,209 + MMJJ_RS04550 0.40 +0.5 849,209 + MMJJ_RS04550 0.40 +0.5
Or see this region's nucleotide sequence