Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01240 and MMJJ_RS01245 overlap by 4 nucleotides MMJJ_RS01245 and MMJJ_RS01250 are separated by 36 nucleotides MMJJ_RS01250 and MMJJ_RS01255 are separated by 350 nucleotides
MMJJ_RS01240: MMJJ_RS01240 - excinuclease ABC subunit UvrC, at 239,108 to 240,691
_RS01240
MMJJ_RS01245: MMJJ_RS01245 - excinuclease ABC subunit UvrB, at 240,688 to 242,628
_RS01245
MMJJ_RS01250: MMJJ_RS01250 - hypothetical protein, at 242,665 to 242,898
_RS01250
MMJJ_RS01255: MMJJ_RS01255 - YqhA family protein, at 243,249 to 243,806
_RS01255
Position (kb) 240
241
242
243 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 239.815 kb on + strand, within MMJJ_RS01240 at 239.866 kb on - strand, within MMJJ_RS01240 at 239.869 kb on + strand, within MMJJ_RS01240 at 239.964 kb on + strand, within MMJJ_RS01240 at 239.966 kb on - strand, within MMJJ_RS01240 at 239.996 kb on + strand at 239.998 kb on + strand, within MMJJ_RS01240 at 239.998 kb on + strand, within MMJJ_RS01240 at 240.032 kb on + strand, within MMJJ_RS01240 at 240.034 kb on - strand, within MMJJ_RS01240 at 240.034 kb on - strand, within MMJJ_RS01240 at 240.042 kb on + strand, within MMJJ_RS01240 at 240.066 kb on + strand, within MMJJ_RS01240 at 240.066 kb on + strand, within MMJJ_RS01240 at 240.101 kb on + strand, within MMJJ_RS01240 at 240.234 kb on - strand, within MMJJ_RS01240 at 240.250 kb on - strand, within MMJJ_RS01240 at 240.275 kb on + strand, within MMJJ_RS01240 at 240.298 kb on - strand, within MMJJ_RS01240 at 240.394 kb on - strand, within MMJJ_RS01240 at 240.402 kb on + strand, within MMJJ_RS01240 at 240.448 kb on - strand, within MMJJ_RS01240 at 240.449 kb on + strand, within MMJJ_RS01240 at 240.449 kb on + strand, within MMJJ_RS01240 at 240.551 kb on - strand at 240.606 kb on - strand at 240.654 kb on + strand at 240.668 kb on + strand at 240.711 kb on + strand at 240.796 kb on + strand at 240.838 kb on + strand at 240.895 kb on + strand, within MMJJ_RS01245 at 240.959 kb on + strand, within MMJJ_RS01245 at 240.974 kb on + strand, within MMJJ_RS01245 at 240.980 kb on + strand, within MMJJ_RS01245 at 240.997 kb on - strand, within MMJJ_RS01245 at 241.000 kb on + strand, within MMJJ_RS01245 at 241.018 kb on - strand, within MMJJ_RS01245 at 241.019 kb on + strand, within MMJJ_RS01245 at 241.020 kb on - strand, within MMJJ_RS01245 at 241.021 kb on + strand, within MMJJ_RS01245 at 241.106 kb on - strand, within MMJJ_RS01245 at 241.106 kb on - strand, within MMJJ_RS01245 at 241.130 kb on - strand, within MMJJ_RS01245 at 241.130 kb on - strand, within MMJJ_RS01245 at 241.130 kb on - strand, within MMJJ_RS01245 at 241.130 kb on - strand, within MMJJ_RS01245 at 241.130 kb on - strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.131 kb on + strand, within MMJJ_RS01245 at 241.132 kb on - strand, within MMJJ_RS01245 at 241.132 kb on - strand, within MMJJ_RS01245 at 241.132 kb on - strand, within MMJJ_RS01245 at 241.132 kb on - strand, within MMJJ_RS01245 at 241.132 kb on - strand, within MMJJ_RS01245 at 241.132 kb on - strand, within MMJJ_RS01245 at 241.133 kb on + strand, within MMJJ_RS01245 at 241.133 kb on + strand, within MMJJ_RS01245 at 241.133 kb on + strand, within MMJJ_RS01245 at 241.133 kb on + strand, within MMJJ_RS01245 at 241.223 kb on - strand, within MMJJ_RS01245 at 241.260 kb on + strand, within MMJJ_RS01245 at 241.295 kb on - strand, within MMJJ_RS01245 at 241.295 kb on - strand, within MMJJ_RS01245 at 241.299 kb on - strand, within MMJJ_RS01245 at 241.417 kb on - strand, within MMJJ_RS01245 at 241.484 kb on - strand, within MMJJ_RS01245 at 241.485 kb on + strand, within MMJJ_RS01245 at 241.509 kb on + strand, within MMJJ_RS01245 at 241.663 kb on - strand, within MMJJ_RS01245 at 241.663 kb on - strand, within MMJJ_RS01245 at 241.688 kb on - strand, within MMJJ_RS01245 at 241.732 kb on - strand, within MMJJ_RS01245 at 241.732 kb on - strand, within MMJJ_RS01245 at 241.733 kb on + strand, within MMJJ_RS01245 at 241.795 kb on - strand, within MMJJ_RS01245 at 241.796 kb on + strand, within MMJJ_RS01245 at 241.796 kb on + strand, within MMJJ_RS01245 at 241.796 kb on + strand, within MMJJ_RS01245 at 241.835 kb on + strand, within MMJJ_RS01245 at 241.835 kb on + strand, within MMJJ_RS01245 at 241.843 kb on + strand, within MMJJ_RS01245 at 241.847 kb on - strand, within MMJJ_RS01245 at 241.883 kb on - strand, within MMJJ_RS01245 at 241.884 kb on + strand, within MMJJ_RS01245 at 241.884 kb on + strand, within MMJJ_RS01245 at 241.888 kb on + strand, within MMJJ_RS01245 at 241.904 kb on - strand, within MMJJ_RS01245 at 241.904 kb on - strand, within MMJJ_RS01245 at 241.905 kb on + strand, within MMJJ_RS01245 at 241.953 kb on + strand, within MMJJ_RS01245 at 241.980 kb on - strand, within MMJJ_RS01245 at 242.027 kb on - strand, within MMJJ_RS01245 at 242.032 kb on + strand, within MMJJ_RS01245 at 242.071 kb on - strand, within MMJJ_RS01245 at 242.118 kb on + strand, within MMJJ_RS01245 at 242.118 kb on + strand, within MMJJ_RS01245 at 242.118 kb on + strand, within MMJJ_RS01245 at 242.118 kb on + strand, within MMJJ_RS01245 at 242.118 kb on + strand, within MMJJ_RS01245 at 242.121 kb on - strand, within MMJJ_RS01245 at 242.237 kb on - strand, within MMJJ_RS01245 at 242.331 kb on + strand, within MMJJ_RS01245 at 242.361 kb on - strand, within MMJJ_RS01245 at 242.447 kb on - strand at 242.468 kb on - strand at 242.486 kb on - strand at 242.545 kb on - strand at 242.546 kb on + strand at 242.631 kb on - strand at 242.649 kb on - strand at 242.678 kb on - strand at 242.679 kb on + strand at 242.693 kb on + strand, within MMJJ_RS01250 at 242.698 kb on - strand, within MMJJ_RS01250 at 242.719 kb on + strand, within MMJJ_RS01250 at 242.719 kb on + strand, within MMJJ_RS01250 at 242.731 kb on - strand, within MMJJ_RS01250 at 242.732 kb on + strand, within MMJJ_RS01250 at 242.732 kb on + strand, within MMJJ_RS01250 at 242.849 kb on - strand, within MMJJ_RS01250 at 242.849 kb on - strand, within MMJJ_RS01250 at 242.849 kb on - strand, within MMJJ_RS01250 at 242.854 kb on - strand, within MMJJ_RS01250 at 242.854 kb on - strand, within MMJJ_RS01250 at 242.890 kb on - strand at 242.891 kb on + strand at 242.920 kb on - strand at 242.934 kb on + strand at 242.937 kb on - strand at 242.940 kb on - strand at 242.940 kb on - strand at 242.941 kb on + strand at 242.941 kb on + strand at 242.942 kb on - strand at 242.943 kb on + strand at 242.943 kb on + strand at 242.945 kb on + strand at 242.945 kb on + strand at 242.945 kb on - strand at 242.966 kb on - strand at 242.966 kb on - strand at 242.966 kb on - strand at 242.969 kb on - strand at 242.969 kb on - strand at 242.969 kb on - strand at 242.970 kb on + strand at 242.971 kb on - strand at 242.972 kb on + strand at 242.986 kb on - strand at 243.019 kb on + strand at 243.025 kb on - strand at 243.025 kb on - strand at 243.026 kb on + strand at 243.030 kb on - strand at 243.030 kb on - strand at 243.031 kb on + strand at 243.031 kb on + strand at 243.036 kb on - strand at 243.037 kb on + strand at 243.103 kb on - strand at 243.103 kb on - strand at 243.103 kb on - strand at 243.118 kb on - strand at 243.121 kb on + strand at 243.121 kb on + strand at 243.121 kb on + strand at 243.123 kb on - strand at 243.123 kb on - strand at 243.124 kb on + strand at 243.124 kb on + strand at 243.124 kb on + strand at 243.124 kb on + strand at 243.147 kb on + strand at 243.160 kb on - strand at 243.165 kb on + strand at 243.168 kb on - strand at 243.169 kb on + strand at 243.169 kb on + strand at 243.173 kb on - strand at 243.174 kb on + strand at 243.177 kb on - strand at 243.177 kb on - strand at 243.178 kb on + strand at 243.214 kb on - strand at 243.217 kb on + strand at 243.224 kb on - strand at 243.225 kb on + strand at 243.236 kb on - strand at 243.236 kb on - strand at 243.248 kb on - strand at 243.293 kb on - strand at 243.296 kb on - strand at 243.296 kb on - strand at 243.297 kb on + strand at 243.353 kb on - strand, within MMJJ_RS01255 at 243.395 kb on - strand, within MMJJ_RS01255 at 243.395 kb on - strand, within MMJJ_RS01255 at 243.441 kb on + strand, within MMJJ_RS01255 at 243.459 kb on + strand, within MMJJ_RS01255 at 243.485 kb on + strand, within MMJJ_RS01255 at 243.487 kb on - strand, within MMJJ_RS01255 at 243.509 kb on + strand, within MMJJ_RS01255 at 243.520 kb on - strand, within MMJJ_RS01255 at 243.523 kb on - strand, within MMJJ_RS01255 at 243.532 kb on - strand, within MMJJ_RS01255 at 243.533 kb on + strand, within MMJJ_RS01255 at 243.545 kb on - strand, within MMJJ_RS01255 at 243.559 kb on + strand, within MMJJ_RS01255 at 243.559 kb on + strand, within MMJJ_RS01255 at 243.580 kb on - strand, within MMJJ_RS01255 at 243.615 kb on + strand, within MMJJ_RS01255 at 243.628 kb on + strand, within MMJJ_RS01255
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 239,815 + MMJJ_RS01240 0.45 +2.4 239,866 - MMJJ_RS01240 0.48 +2.7 239,869 + MMJJ_RS01240 0.48 -2.4 239,964 + MMJJ_RS01240 0.54 +0.9 239,966 - MMJJ_RS01240 0.54 -2.9 239,996 + +0.9 239,998 + MMJJ_RS01240 0.56 -2.6 239,998 + MMJJ_RS01240 0.56 +0.2 240,032 + MMJJ_RS01240 0.58 -0.9 240,034 - MMJJ_RS01240 0.58 -1.8 240,034 - MMJJ_RS01240 0.58 -2.1 240,042 + MMJJ_RS01240 0.59 +0.8 240,066 + MMJJ_RS01240 0.60 +0.7 240,066 + MMJJ_RS01240 0.60 +2.4 240,101 + MMJJ_RS01240 0.63 -3.2 240,234 - MMJJ_RS01240 0.71 +0.0 240,250 - MMJJ_RS01240 0.72 -4.1 240,275 + MMJJ_RS01240 0.74 +3.8 240,298 - MMJJ_RS01240 0.75 -0.5 240,394 - MMJJ_RS01240 0.81 +0.3 240,402 + MMJJ_RS01240 0.82 +2.2 240,448 - MMJJ_RS01240 0.85 -2.4 240,449 + MMJJ_RS01240 0.85 -3.8 240,449 + MMJJ_RS01240 0.85 -2.3 240,551 - +0.8 240,606 - +1.6 240,654 + -0.6 240,668 + -2.0 240,711 + -0.1 240,796 + +0.5 240,838 + -0.1 240,895 + MMJJ_RS01245 0.11 +1.0 240,959 + MMJJ_RS01245 0.14 +1.5 240,974 + MMJJ_RS01245 0.15 +2.3 240,980 + MMJJ_RS01245 0.15 -1.6 240,997 - MMJJ_RS01245 0.16 +3.2 241,000 + MMJJ_RS01245 0.16 +4.2 241,018 - MMJJ_RS01245 0.17 -0.6 241,019 + MMJJ_RS01245 0.17 -1.2 241,020 - MMJJ_RS01245 0.17 +0.6 241,021 + MMJJ_RS01245 0.17 -3.6 241,106 - MMJJ_RS01245 0.22 +0.2 241,106 - MMJJ_RS01245 0.22 -2.9 241,130 - MMJJ_RS01245 0.23 -1.0 241,130 - MMJJ_RS01245 0.23 -1.8 241,130 - MMJJ_RS01245 0.23 -2.0 241,130 - MMJJ_RS01245 0.23 -1.1 241,130 - MMJJ_RS01245 0.23 +1.2 241,131 + MMJJ_RS01245 0.23 -2.1 241,131 + MMJJ_RS01245 0.23 -2.4 241,131 + MMJJ_RS01245 0.23 -1.2 241,131 + MMJJ_RS01245 0.23 -0.3 241,131 + MMJJ_RS01245 0.23 -0.1 241,131 + MMJJ_RS01245 0.23 -2.2 241,131 + MMJJ_RS01245 0.23 -0.6 241,132 - MMJJ_RS01245 0.23 +1.4 241,132 - MMJJ_RS01245 0.23 -1.5 241,132 - MMJJ_RS01245 0.23 -0.4 241,132 - MMJJ_RS01245 0.23 -1.5 241,132 - MMJJ_RS01245 0.23 -2.1 241,132 - MMJJ_RS01245 0.23 -1.6 241,133 + MMJJ_RS01245 0.23 -1.2 241,133 + MMJJ_RS01245 0.23 -1.5 241,133 + MMJJ_RS01245 0.23 -0.7 241,133 + MMJJ_RS01245 0.23 +2.7 241,223 - MMJJ_RS01245 0.28 +1.4 241,260 + MMJJ_RS01245 0.29 -1.0 241,295 - MMJJ_RS01245 0.31 -0.7 241,295 - MMJJ_RS01245 0.31 -0.0 241,299 - MMJJ_RS01245 0.31 -0.9 241,417 - MMJJ_RS01245 0.38 -3.4 241,484 - MMJJ_RS01245 0.41 +1.5 241,485 + MMJJ_RS01245 0.41 +0.0 241,509 + MMJJ_RS01245 0.42 -0.3 241,663 - MMJJ_RS01245 0.50 -2.9 241,663 - MMJJ_RS01245 0.50 +0.7 241,688 - MMJJ_RS01245 0.52 -3.6 241,732 - MMJJ_RS01245 0.54 -0.8 241,732 - MMJJ_RS01245 0.54 +0.2 241,733 + MMJJ_RS01245 0.54 -2.0 241,795 - MMJJ_RS01245 0.57 -3.2 241,796 + MMJJ_RS01245 0.57 -0.2 241,796 + MMJJ_RS01245 0.57 +0.3 241,796 + MMJJ_RS01245 0.57 -0.7 241,835 + MMJJ_RS01245 0.59 -2.3 241,835 + MMJJ_RS01245 0.59 +1.9 241,843 + MMJJ_RS01245 0.60 -0.4 241,847 - MMJJ_RS01245 0.60 +2.6 241,883 - MMJJ_RS01245 0.62 +1.9 241,884 + MMJJ_RS01245 0.62 +1.5 241,884 + MMJJ_RS01245 0.62 +2.4 241,888 + MMJJ_RS01245 0.62 +1.0 241,904 - MMJJ_RS01245 0.63 +1.3 241,904 - MMJJ_RS01245 0.63 +1.4 241,905 + MMJJ_RS01245 0.63 -0.4 241,953 + MMJJ_RS01245 0.65 +1.3 241,980 - MMJJ_RS01245 0.67 +1.3 242,027 - MMJJ_RS01245 0.69 +2.1 242,032 + MMJJ_RS01245 0.69 -1.7 242,071 - MMJJ_RS01245 0.71 -3.5 242,118 + MMJJ_RS01245 0.74 -3.5 242,118 + MMJJ_RS01245 0.74 +2.2 242,118 + MMJJ_RS01245 0.74 +1.8 242,118 + MMJJ_RS01245 0.74 -2.8 242,118 + MMJJ_RS01245 0.74 +0.8 242,121 - MMJJ_RS01245 0.74 -0.7 242,237 - MMJJ_RS01245 0.80 -3.8 242,331 + MMJJ_RS01245 0.85 +0.6 242,361 - MMJJ_RS01245 0.86 +1.5 242,447 - -2.1 242,468 - -1.7 242,486 - -2.9 242,545 - -1.9 242,546 + +3.1 242,631 - +0.1 242,649 - +0.5 242,678 - +2.1 242,679 + -3.3 242,693 + MMJJ_RS01250 0.12 -1.7 242,698 - MMJJ_RS01250 0.14 -4.0 242,719 + MMJJ_RS01250 0.23 +1.6 242,719 + MMJJ_RS01250 0.23 -3.2 242,731 - MMJJ_RS01250 0.28 -0.2 242,732 + MMJJ_RS01250 0.29 -1.7 242,732 + MMJJ_RS01250 0.29 +0.7 242,849 - MMJJ_RS01250 0.79 -0.1 242,849 - MMJJ_RS01250 0.79 -3.7 242,849 - MMJJ_RS01250 0.79 -0.3 242,854 - MMJJ_RS01250 0.81 -1.7 242,854 - MMJJ_RS01250 0.81 -2.3 242,890 - +2.6 242,891 + +0.5 242,920 - +2.0 242,934 + -0.3 242,937 - -0.9 242,940 - -3.0 242,940 - -1.9 242,941 + +1.5 242,941 + -2.3 242,942 - -3.2 242,943 + +0.2 242,943 + +0.8 242,945 + -0.3 242,945 + +2.0 242,945 - -1.9 242,966 - +1.9 242,966 - +0.3 242,966 - -0.0 242,969 - +1.6 242,969 - +0.5 242,969 - -0.9 242,970 + +0.7 242,971 - -2.3 242,972 + -2.8 242,986 - +1.5 243,019 + +1.1 243,025 - -0.7 243,025 - +0.0 243,026 + +0.9 243,030 - -0.7 243,030 - +2.6 243,031 + +2.1 243,031 + -0.9 243,036 - -0.1 243,037 + -1.6 243,103 - -2.1 243,103 - -2.4 243,103 - -0.5 243,118 - -1.1 243,121 + +2.6 243,121 + -0.1 243,121 + +1.3 243,123 - +0.1 243,123 - +2.5 243,124 + -1.6 243,124 + +1.1 243,124 + +0.5 243,124 + +3.0 243,147 + -3.5 243,160 - +0.8 243,165 + -0.3 243,168 - +1.9 243,169 + -1.6 243,169 + -1.6 243,173 - -4.2 243,174 + +1.5 243,177 - +3.2 243,177 - -0.2 243,178 + +0.4 243,214 - -3.6 243,217 + +0.1 243,224 - -1.5 243,225 + +0.9 243,236 - +2.8 243,236 - -1.4 243,248 - +0.9 243,293 - +3.3 243,296 - -1.3 243,296 - -1.6 243,297 + +2.6 243,353 - MMJJ_RS01255 0.19 +1.2 243,395 - MMJJ_RS01255 0.26 -1.3 243,395 - MMJJ_RS01255 0.26 -2.0 243,441 + MMJJ_RS01255 0.34 +1.5 243,459 + MMJJ_RS01255 0.38 -0.2 243,485 + MMJJ_RS01255 0.42 -1.7 243,487 - MMJJ_RS01255 0.43 -0.5 243,509 + MMJJ_RS01255 0.47 +1.9 243,520 - MMJJ_RS01255 0.49 +3.2 243,523 - MMJJ_RS01255 0.49 +0.4 243,532 - MMJJ_RS01255 0.51 -0.5 243,533 + MMJJ_RS01255 0.51 +2.9 243,545 - MMJJ_RS01255 0.53 +0.4 243,559 + MMJJ_RS01255 0.56 +1.3 243,559 + MMJJ_RS01255 0.56 +0.3 243,580 - MMJJ_RS01255 0.59 +1.3 243,615 + MMJJ_RS01255 0.66 +0.8 243,628 + MMJJ_RS01255 0.68 +0.4
Or see this region's nucleotide sequence