Experiment: McCas_formate_complete Irgasan 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00825 and MMJJ_RS00830 are separated by 24 nucleotides MMJJ_RS00830 and MMJJ_RS00835 are separated by 267 nucleotides MMJJ_RS00835 and MMJJ_RS00840 are separated by 14 nucleotides MMJJ_RS00840 and MMJJ_RS00845 are separated by 281 nucleotides
MMJJ_RS00825: MMJJ_RS00825 - uroporphyrinogen decarboxylase family protein, at 157,554 to 158,645
_RS00825
MMJJ_RS00830: MMJJ_RS00830 - corrinoid protein, at 158,670 to 159,329
_RS00830
MMJJ_RS00835: MMJJ_RS00835 - hypothetical protein, at 159,597 to 159,941
_RS00835
MMJJ_RS00840: MMJJ_RS00840 - hypothetical protein, at 159,956 to 160,249
_RS00840
MMJJ_RS00845: MMJJ_RS00845 - hypothetical protein, at 160,531 to 160,797
_RS00845
Position (kb) 159
160 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 158.646 kb on + strand at 158.680 kb on + strand at 158.732 kb on - strand at 158.735 kb on + strand at 158.735 kb on + strand at 158.827 kb on + strand, within MMJJ_RS00830 at 158.945 kb on - strand, within MMJJ_RS00830 at 158.956 kb on - strand, within MMJJ_RS00830 at 158.995 kb on + strand, within MMJJ_RS00830 at 159.048 kb on + strand, within MMJJ_RS00830 at 159.076 kb on + strand, within MMJJ_RS00830 at 159.076 kb on + strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.107 kb on + strand, within MMJJ_RS00830 at 159.107 kb on + strand, within MMJJ_RS00830 at 159.107 kb on + strand, within MMJJ_RS00830 at 159.127 kb on + strand, within MMJJ_RS00830 at 159.130 kb on + strand, within MMJJ_RS00830 at 159.130 kb on + strand, within MMJJ_RS00830 at 159.238 kb on - strand, within MMJJ_RS00830 at 159.250 kb on + strand, within MMJJ_RS00830 at 159.276 kb on + strand at 159.303 kb on - strand at 159.304 kb on + strand at 159.304 kb on + strand at 159.304 kb on + strand at 159.354 kb on + strand at 159.475 kb on - strand at 159.490 kb on - strand at 159.538 kb on - strand at 159.538 kb on - strand at 159.538 kb on - strand at 159.539 kb on + strand at 159.539 kb on + strand at 159.539 kb on + strand at 159.541 kb on - strand at 159.541 kb on - strand at 159.542 kb on + strand at 159.542 kb on + strand at 159.542 kb on + strand at 159.544 kb on - strand at 159.544 kb on - strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.546 kb on - strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.549 kb on - strand at 159.549 kb on - strand at 159.550 kb on + strand at 159.550 kb on + strand at 159.550 kb on + strand at 159.551 kb on - strand at 159.551 kb on - strand at 159.551 kb on - strand at 159.559 kb on - strand at 159.559 kb on - strand at 159.560 kb on + strand at 159.560 kb on + strand at 159.560 kb on + strand at 159.561 kb on - strand at 159.576 kb on - strand at 159.581 kb on - strand at 159.581 kb on - strand at 159.581 kb on - strand at 159.581 kb on - strand at 159.582 kb on + strand at 159.586 kb on + strand at 159.646 kb on + strand, within MMJJ_RS00835 at 159.647 kb on - strand, within MMJJ_RS00835 at 159.647 kb on - strand, within MMJJ_RS00835 at 159.647 kb on - strand, within MMJJ_RS00835 at 159.648 kb on + strand, within MMJJ_RS00835 at 159.648 kb on + strand, within MMJJ_RS00835 at 159.648 kb on + strand, within MMJJ_RS00835 at 159.705 kb on + strand, within MMJJ_RS00835 at 159.728 kb on - strand, within MMJJ_RS00835 at 159.730 kb on - strand, within MMJJ_RS00835 at 159.731 kb on + strand, within MMJJ_RS00835 at 159.731 kb on + strand, within MMJJ_RS00835 at 159.733 kb on - strand, within MMJJ_RS00835 at 159.734 kb on + strand, within MMJJ_RS00835 at 159.734 kb on + strand, within MMJJ_RS00835 at 159.734 kb on + strand, within MMJJ_RS00835 at 159.741 kb on - strand, within MMJJ_RS00835 at 159.742 kb on + strand, within MMJJ_RS00835 at 159.742 kb on + strand, within MMJJ_RS00835 at 159.764 kb on + strand, within MMJJ_RS00835 at 159.764 kb on + strand, within MMJJ_RS00835 at 159.822 kb on - strand, within MMJJ_RS00835 at 159.887 kb on + strand, within MMJJ_RS00835 at 159.923 kb on - strand at 159.940 kb on + strand at 159.944 kb on - strand at 159.945 kb on + strand at 159.958 kb on + strand at 159.989 kb on + strand, within MMJJ_RS00840 at 160.049 kb on + strand, within MMJJ_RS00840 at 160.062 kb on + strand, within MMJJ_RS00840 at 160.109 kb on + strand, within MMJJ_RS00840 at 160.113 kb on - strand, within MMJJ_RS00840 at 160.128 kb on + strand, within MMJJ_RS00840 at 160.158 kb on + strand, within MMJJ_RS00840 at 160.288 kb on - strand at 160.288 kb on - strand at 160.288 kb on - strand at 160.289 kb on + strand at 160.290 kb on - strand at 160.290 kb on - strand at 160.290 kb on - strand at 160.291 kb on + strand at 160.291 kb on + strand at 160.300 kb on + strand at 160.320 kb on - strand at 160.321 kb on + strand at 160.321 kb on + strand at 160.321 kb on + strand at 160.322 kb on - strand at 160.322 kb on - strand at 160.322 kb on - strand at 160.322 kb on - strand at 160.322 kb on - strand at 160.323 kb on + strand at 160.323 kb on + strand at 160.323 kb on + strand at 160.323 kb on + strand at 160.328 kb on + strand at 160.407 kb on - strand at 160.411 kb on - strand at 160.411 kb on - strand at 160.411 kb on - strand at 160.411 kb on - strand at 160.411 kb on - strand at 160.411 kb on - strand at 160.411 kb on - strand at 160.412 kb on + strand at 160.412 kb on + strand at 160.412 kb on + strand at 160.412 kb on + strand at 160.412 kb on + strand at 160.414 kb on - strand at 160.414 kb on - strand at 160.414 kb on - strand at 160.414 kb on - strand at 160.414 kb on - strand at 160.414 kb on - strand at 160.414 kb on - strand at 160.415 kb on + strand at 160.415 kb on + strand at 160.415 kb on + strand at 160.415 kb on + strand at 160.415 kb on + strand at 160.415 kb on + strand at 160.415 kb on + strand at 160.417 kb on + strand at 160.434 kb on - strand at 160.438 kb on - strand at 160.445 kb on - strand at 160.445 kb on - strand at 160.445 kb on - strand at 160.445 kb on - strand at 160.445 kb on - strand at 160.446 kb on + strand at 160.446 kb on + strand at 160.446 kb on + strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.447 kb on - strand at 160.448 kb on + strand at 160.448 kb on + strand at 160.448 kb on + strand at 160.448 kb on + strand at 160.448 kb on + strand at 160.448 kb on + strand at 160.449 kb on - strand at 160.449 kb on - strand at 160.449 kb on - strand at 160.543 kb on + strand at 160.548 kb on - strand at 160.557 kb on - strand at 160.558 kb on + strand, within MMJJ_RS00845 at 160.563 kb on + strand, within MMJJ_RS00845 at 160.567 kb on - strand, within MMJJ_RS00845 at 160.571 kb on - strand, within MMJJ_RS00845 at 160.592 kb on - strand, within MMJJ_RS00845 at 160.599 kb on - strand, within MMJJ_RS00845 at 160.608 kb on - strand, within MMJJ_RS00845 at 160.608 kb on - strand, within MMJJ_RS00845 at 160.609 kb on + strand, within MMJJ_RS00845 at 160.609 kb on + strand, within MMJJ_RS00845 at 160.615 kb on + strand, within MMJJ_RS00845 at 160.640 kb on - strand, within MMJJ_RS00845 at 160.646 kb on + strand, within MMJJ_RS00845 at 160.646 kb on + strand, within MMJJ_RS00845 at 160.669 kb on + strand, within MMJJ_RS00845 at 160.680 kb on + strand, within MMJJ_RS00845 at 160.680 kb on + strand, within MMJJ_RS00845 at 160.680 kb on + strand, within MMJJ_RS00845 at 160.683 kb on + strand, within MMJJ_RS00845 at 160.704 kb on - strand, within MMJJ_RS00845 at 160.711 kb on - strand, within MMJJ_RS00845 at 160.711 kb on - strand, within MMJJ_RS00845 at 160.711 kb on - strand, within MMJJ_RS00845 at 160.715 kb on + strand, within MMJJ_RS00845 at 160.724 kb on - strand, within MMJJ_RS00845 at 160.728 kb on - strand, within MMJJ_RS00845 at 160.729 kb on + strand, within MMJJ_RS00845 at 160.729 kb on + strand, within MMJJ_RS00845 at 160.745 kb on - strand, within MMJJ_RS00845 at 160.778 kb on - strand at 160.845 kb on + strand at 160.849 kb on - strand at 160.850 kb on + strand at 160.850 kb on + strand at 160.850 kb on + strand at 160.850 kb on + strand at 160.877 kb on + strand at 160.879 kb on - strand at 160.880 kb on + strand at 160.880 kb on + strand at 160.880 kb on + strand at 160.880 kb on + strand at 160.881 kb on - strand at 160.881 kb on - strand at 160.881 kb on - strand at 160.882 kb on + strand at 160.882 kb on + strand at 160.882 kb on + strand at 160.901 kb on - strand at 160.901 kb on - strand at 160.904 kb on - strand at 160.908 kb on - strand at 160.918 kb on + strand at 160.928 kb on - strand at 160.929 kb on + strand at 160.929 kb on + strand at 160.929 kb on + strand at 160.929 kb on + strand at 160.930 kb on - strand at 160.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Irgasan 0.0125 mM remove 158,646 + -3.5 158,680 + -3.3 158,732 - -1.0 158,735 + +2.1 158,735 + -2.8 158,827 + MMJJ_RS00830 0.24 -1.0 158,945 - MMJJ_RS00830 0.42 +0.3 158,956 - MMJJ_RS00830 0.43 +0.3 158,995 + MMJJ_RS00830 0.49 -2.0 159,048 + MMJJ_RS00830 0.57 -4.4 159,076 + MMJJ_RS00830 0.62 +1.4 159,076 + MMJJ_RS00830 0.62 -0.5 159,106 - MMJJ_RS00830 0.66 -2.4 159,106 - MMJJ_RS00830 0.66 +0.9 159,106 - MMJJ_RS00830 0.66 -0.4 159,106 - MMJJ_RS00830 0.66 +1.3 159,107 + MMJJ_RS00830 0.66 +2.4 159,107 + MMJJ_RS00830 0.66 -3.0 159,107 + MMJJ_RS00830 0.66 -2.1 159,127 + MMJJ_RS00830 0.69 -1.1 159,130 + MMJJ_RS00830 0.70 +2.1 159,130 + MMJJ_RS00830 0.70 -2.4 159,238 - MMJJ_RS00830 0.86 +0.4 159,250 + MMJJ_RS00830 0.88 -2.0 159,276 + -3.1 159,303 - +3.2 159,304 + -0.3 159,304 + -3.1 159,304 + +1.3 159,354 + -3.0 159,475 - -3.4 159,490 - +2.5 159,538 - -4.5 159,538 - -1.0 159,538 - -1.4 159,539 + +0.0 159,539 + +0.9 159,539 + +1.1 159,541 - +2.6 159,541 - -1.9 159,542 + -2.0 159,542 + -0.8 159,542 + -1.2 159,544 - -0.1 159,544 - -0.1 159,545 + -2.5 159,545 + +1.4 159,545 + +2.5 159,545 + -3.5 159,545 + -2.2 159,545 + +1.8 159,546 - -0.9 159,547 + -2.2 159,547 + +1.1 159,547 + -1.6 159,547 + -2.9 159,549 - -0.5 159,549 - -0.3 159,550 + -1.3 159,550 + +1.4 159,550 + -2.6 159,551 - -1.5 159,551 - +0.3 159,551 - -0.6 159,559 - +0.4 159,559 - +0.6 159,560 + -0.5 159,560 + +2.0 159,560 + +3.5 159,561 - -1.1 159,576 - +1.2 159,581 - -1.5 159,581 - -3.4 159,581 - +0.5 159,581 - +2.6 159,582 + +0.9 159,586 + -1.9 159,646 + MMJJ_RS00835 0.14 +0.5 159,647 - MMJJ_RS00835 0.14 +0.9 159,647 - MMJJ_RS00835 0.14 +0.5 159,647 - MMJJ_RS00835 0.14 -1.0 159,648 + MMJJ_RS00835 0.15 +0.6 159,648 + MMJJ_RS00835 0.15 -0.7 159,648 + MMJJ_RS00835 0.15 +0.5 159,705 + MMJJ_RS00835 0.31 +0.1 159,728 - MMJJ_RS00835 0.38 -3.5 159,730 - MMJJ_RS00835 0.39 -3.2 159,731 + MMJJ_RS00835 0.39 +0.3 159,731 + MMJJ_RS00835 0.39 +0.7 159,733 - MMJJ_RS00835 0.39 -3.0 159,734 + MMJJ_RS00835 0.40 -3.6 159,734 + MMJJ_RS00835 0.40 -1.6 159,734 + MMJJ_RS00835 0.40 +0.6 159,741 - MMJJ_RS00835 0.42 -3.2 159,742 + MMJJ_RS00835 0.42 +0.7 159,742 + MMJJ_RS00835 0.42 -1.3 159,764 + MMJJ_RS00835 0.48 -1.8 159,764 + MMJJ_RS00835 0.48 -0.1 159,822 - MMJJ_RS00835 0.65 -0.1 159,887 + MMJJ_RS00835 0.84 +0.0 159,923 - +1.7 159,940 + +0.3 159,944 - +3.2 159,945 + +1.1 159,958 + -1.0 159,989 + MMJJ_RS00840 0.11 +1.6 160,049 + MMJJ_RS00840 0.32 +1.1 160,062 + MMJJ_RS00840 0.36 -1.4 160,109 + MMJJ_RS00840 0.52 -2.0 160,113 - MMJJ_RS00840 0.53 -0.2 160,128 + MMJJ_RS00840 0.59 -0.8 160,158 + MMJJ_RS00840 0.69 -0.2 160,288 - -1.0 160,288 - +1.3 160,288 - +2.3 160,289 + -2.2 160,290 - -0.6 160,290 - -0.9 160,290 - -2.1 160,291 + -1.6 160,291 + -1.5 160,300 + +0.7 160,320 - +0.5 160,321 + +3.3 160,321 + -2.4 160,321 + -0.7 160,322 - -5.0 160,322 - -0.3 160,322 - +5.0 160,322 - -2.6 160,322 - -1.1 160,323 + -3.5 160,323 + -2.5 160,323 + -3.1 160,323 + +3.7 160,328 + +1.4 160,407 - -0.1 160,411 - +0.8 160,411 - +0.8 160,411 - -1.9 160,411 - +0.4 160,411 - +1.3 160,411 - +2.0 160,411 - +5.1 160,412 + +1.3 160,412 + -0.6 160,412 + +1.0 160,412 + +1.8 160,412 + -2.4 160,414 - +0.9 160,414 - -0.5 160,414 - -3.4 160,414 - +4.0 160,414 - -3.0 160,414 - +3.0 160,414 - -1.7 160,415 + -1.1 160,415 + +1.2 160,415 + -0.3 160,415 + -1.3 160,415 + +2.0 160,415 + -2.9 160,415 + -2.8 160,417 + -3.5 160,434 - +2.4 160,438 - +2.1 160,445 - +1.2 160,445 - +1.4 160,445 - +0.7 160,445 - +1.7 160,445 - -1.3 160,446 + -1.7 160,446 + +0.0 160,446 + -1.9 160,447 - -0.5 160,447 - +1.6 160,447 - -3.0 160,447 - +1.5 160,447 - +0.5 160,447 - +1.6 160,447 - -3.0 160,447 - -5.0 160,447 - +2.1 160,448 + -2.6 160,448 + +1.7 160,448 + +1.5 160,448 + -1.5 160,448 + +2.5 160,448 + +0.0 160,449 - +1.5 160,449 - -2.8 160,449 - +4.9 160,543 + -0.1 160,548 - +0.8 160,557 - -1.1 160,558 + MMJJ_RS00845 0.10 -2.5 160,563 + MMJJ_RS00845 0.12 +0.3 160,567 - MMJJ_RS00845 0.13 +3.3 160,571 - MMJJ_RS00845 0.15 +0.2 160,592 - MMJJ_RS00845 0.23 +5.2 160,599 - MMJJ_RS00845 0.25 +1.7 160,608 - MMJJ_RS00845 0.29 -0.4 160,608 - MMJJ_RS00845 0.29 +2.3 160,609 + MMJJ_RS00845 0.29 -0.1 160,609 + MMJJ_RS00845 0.29 +0.2 160,615 + MMJJ_RS00845 0.31 +2.0 160,640 - MMJJ_RS00845 0.41 -1.8 160,646 + MMJJ_RS00845 0.43 -1.1 160,646 + MMJJ_RS00845 0.43 +2.7 160,669 + MMJJ_RS00845 0.52 -0.4 160,680 + MMJJ_RS00845 0.56 +1.7 160,680 + MMJJ_RS00845 0.56 +2.3 160,680 + MMJJ_RS00845 0.56 -3.3 160,683 + MMJJ_RS00845 0.57 +0.4 160,704 - MMJJ_RS00845 0.65 -0.1 160,711 - MMJJ_RS00845 0.67 +2.0 160,711 - MMJJ_RS00845 0.67 -3.7 160,711 - MMJJ_RS00845 0.67 +0.9 160,715 + MMJJ_RS00845 0.69 -0.0 160,724 - MMJJ_RS00845 0.72 +2.6 160,728 - MMJJ_RS00845 0.74 +0.1 160,729 + MMJJ_RS00845 0.74 +1.4 160,729 + MMJJ_RS00845 0.74 -1.8 160,745 - MMJJ_RS00845 0.80 -1.8 160,778 - -4.3 160,845 + +3.5 160,849 - -0.8 160,850 + -4.9 160,850 + -2.0 160,850 + -1.8 160,850 + -1.5 160,877 + -0.3 160,879 - +0.2 160,880 + -2.0 160,880 + -1.2 160,880 + +0.2 160,880 + +2.3 160,881 - +1.7 160,881 - +1.1 160,881 - -0.4 160,882 + +0.6 160,882 + -1.5 160,882 + +1.0 160,901 - -3.1 160,901 - -2.6 160,904 - -3.1 160,908 - +2.2 160,918 + +0.1 160,928 - +2.4 160,929 + -0.6 160,929 + -0.1 160,929 + +1.1 160,929 + -0.5 160,930 - -2.3 160,937 - +3.2
Or see this region's nucleotide sequence