Experiment: McCas_formate_complete hypophosphite 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08600 and MMJJ_RS08605 are separated by 59 nucleotides MMJJ_RS08605 and MMJJ_RS08610 are separated by 4 nucleotides MMJJ_RS08610 and MMJJ_RS08615 overlap by 14 nucleotides
MMJJ_RS08600: MMJJ_RS08600 - transketolase family protein, at 1,583,638 to 1,584,576
_RS08600
MMJJ_RS08605: MMJJ_RS08605 - hypothetical protein, at 1,584,636 to 1,585,391
_RS08605
MMJJ_RS08610: MMJJ_RS08610 - oligosaccharide repeat unit polymerase family protein, at 1,585,396 to 1,586,469
_RS08610
MMJJ_RS08615: MMJJ_RS08615 - oligosaccharide repeat unit polymerase family protein, at 1,586,456 to 1,587,631
_RS08615
Position (kb)
1585
1586
1587 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1584.599 kb on - strand at 1584.604 kb on + strand at 1584.604 kb on + strand at 1584.976 kb on + strand, within MMJJ_RS08605 at 1585.005 kb on + strand, within MMJJ_RS08605 at 1585.009 kb on + strand at 1585.726 kb on + strand, within MMJJ_RS08610 at 1586.417 kb on - strand at 1586.417 kb on - strand at 1586.533 kb on + strand at 1586.628 kb on - strand, within MMJJ_RS08615 at 1586.629 kb on + strand, within MMJJ_RS08615 at 1586.691 kb on - strand, within MMJJ_RS08615 at 1586.775 kb on - strand, within MMJJ_RS08615 at 1586.805 kb on - strand, within MMJJ_RS08615 at 1586.806 kb on + strand, within MMJJ_RS08615 at 1586.838 kb on + strand, within MMJJ_RS08615 at 1586.871 kb on - strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.881 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.905 kb on + strand, within MMJJ_RS08615 at 1586.967 kb on - strand, within MMJJ_RS08615 at 1587.020 kb on - strand, within MMJJ_RS08615 at 1587.031 kb on + strand, within MMJJ_RS08615 at 1587.081 kb on - strand, within MMJJ_RS08615 at 1587.082 kb on + strand, within MMJJ_RS08615 at 1587.103 kb on - strand, within MMJJ_RS08615 at 1587.153 kb on - strand, within MMJJ_RS08615 at 1587.154 kb on + strand, within MMJJ_RS08615 at 1587.157 kb on - strand at 1587.273 kb on - strand, within MMJJ_RS08615 at 1587.274 kb on + strand, within MMJJ_RS08615 at 1587.283 kb on - strand, within MMJJ_RS08615 at 1587.286 kb on + strand, within MMJJ_RS08615 at 1587.323 kb on + strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.354 kb on - strand, within MMJJ_RS08615 at 1587.373 kb on - strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.374 kb on + strand, within MMJJ_RS08615 at 1587.376 kb on - strand, within MMJJ_RS08615 at 1587.377 kb on + strand, within MMJJ_RS08615 at 1587.380 kb on + strand, within MMJJ_RS08615 at 1587.405 kb on + strand, within MMJJ_RS08615 at 1587.429 kb on - strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.445 kb on + strand, within MMJJ_RS08615 at 1587.460 kb on - strand, within MMJJ_RS08615 at 1587.465 kb on - strand, within MMJJ_RS08615 at 1587.466 kb on + strand, within MMJJ_RS08615
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete hypophosphite 62.5 mM remove 1,584,599 - -0.5 1,584,604 + -3.1 1,584,604 + -2.7 1,584,976 + MMJJ_RS08605 0.45 -1.5 1,585,005 + MMJJ_RS08605 0.49 +1.0 1,585,009 + -0.6 1,585,726 + MMJJ_RS08610 0.31 -1.2 1,586,417 - -0.8 1,586,417 - -0.5 1,586,533 + -0.5 1,586,628 - MMJJ_RS08615 0.15 +1.0 1,586,629 + MMJJ_RS08615 0.15 -3.3 1,586,691 - MMJJ_RS08615 0.20 -0.8 1,586,775 - MMJJ_RS08615 0.27 +0.4 1,586,805 - MMJJ_RS08615 0.30 +0.8 1,586,806 + MMJJ_RS08615 0.30 -0.4 1,586,838 + MMJJ_RS08615 0.32 -0.1 1,586,871 - MMJJ_RS08615 0.35 +2.2 1,586,881 + MMJJ_RS08615 0.36 -1.8 1,586,881 + MMJJ_RS08615 0.36 -2.8 1,586,905 + MMJJ_RS08615 0.38 -1.3 1,586,905 + MMJJ_RS08615 0.38 +2.3 1,586,967 - MMJJ_RS08615 0.43 -2.6 1,587,020 - MMJJ_RS08615 0.48 -1.0 1,587,031 + MMJJ_RS08615 0.49 -0.1 1,587,081 - MMJJ_RS08615 0.53 -1.2 1,587,082 + MMJJ_RS08615 0.53 -1.9 1,587,103 - MMJJ_RS08615 0.55 +0.4 1,587,153 - MMJJ_RS08615 0.59 -0.4 1,587,154 + MMJJ_RS08615 0.59 -2.7 1,587,157 - -0.6 1,587,273 - MMJJ_RS08615 0.69 -2.7 1,587,274 + MMJJ_RS08615 0.70 -1.3 1,587,283 - MMJJ_RS08615 0.70 -3.8 1,587,286 + MMJJ_RS08615 0.71 -2.0 1,587,323 + MMJJ_RS08615 0.74 -2.1 1,587,354 - MMJJ_RS08615 0.76 +0.2 1,587,354 - MMJJ_RS08615 0.76 -1.2 1,587,373 - MMJJ_RS08615 0.78 +2.0 1,587,374 + MMJJ_RS08615 0.78 -3.5 1,587,374 + MMJJ_RS08615 0.78 -3.0 1,587,374 + MMJJ_RS08615 0.78 +0.4 1,587,376 - MMJJ_RS08615 0.78 -0.1 1,587,377 + MMJJ_RS08615 0.78 -0.0 1,587,380 + MMJJ_RS08615 0.79 -2.1 1,587,405 + MMJJ_RS08615 0.81 +0.4 1,587,429 - MMJJ_RS08615 0.83 +1.6 1,587,445 + MMJJ_RS08615 0.84 -0.1 1,587,445 + MMJJ_RS08615 0.84 +0.4 1,587,460 - MMJJ_RS08615 0.85 -1.2 1,587,465 - MMJJ_RS08615 0.86 -3.0 1,587,466 + MMJJ_RS08615 0.86 -1.6
Or see this region's nucleotide sequence