Experiment: McCas_formate_complete hypophosphite 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08520 and MMJJ_RS08525 are separated by 41 nucleotides MMJJ_RS08525 and MMJJ_RS08530 are separated by 42 nucleotides MMJJ_RS08530 and MMJJ_RS08535 are separated by 50 nucleotides MMJJ_RS08535 and MMJJ_RS08540 are separated by 52 nucleotides
MMJJ_RS08520: MMJJ_RS08520 - peptidylprolyl isomerase, at 1,567,533 to 1,568,021
_RS08520
MMJJ_RS08525: MMJJ_RS08525 - peptidylprolyl isomerase, at 1,568,063 to 1,568,665
_RS08525
MMJJ_RS08530: MMJJ_RS08530 - RNA-binding transcriptional accessory protein, at 1,568,708 to 1,570,849
_RS08530
MMJJ_RS08535: MMJJ_RS08535 - NOL1/NOP2/sun family putative RNA methylase, at 1,570,900 to 1,571,697
_RS08535
MMJJ_RS08540: MMJJ_RS08540 - archaeosortase family protein ArtE, at 1,571,750 to 1,572,223
_RS08540
Position (kb)
1568
1569
1570
1571 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1567.808 kb on + strand, within MMJJ_RS08520 at 1567.835 kb on + strand, within MMJJ_RS08520 at 1567.966 kb on + strand, within MMJJ_RS08520 at 1568.010 kb on + strand at 1568.037 kb on - strand at 1568.038 kb on + strand at 1568.089 kb on + strand at 1568.132 kb on + strand, within MMJJ_RS08525 at 1568.172 kb on - strand, within MMJJ_RS08525 at 1568.173 kb on + strand, within MMJJ_RS08525 at 1568.178 kb on + strand, within MMJJ_RS08525 at 1568.310 kb on - strand at 1568.311 kb on + strand, within MMJJ_RS08525 at 1568.448 kb on - strand, within MMJJ_RS08525 at 1568.449 kb on + strand, within MMJJ_RS08525 at 1568.496 kb on + strand, within MMJJ_RS08525 at 1568.542 kb on - strand, within MMJJ_RS08525 at 1568.600 kb on + strand, within MMJJ_RS08525 at 1568.634 kb on - strand at 1568.635 kb on + strand at 1568.650 kb on - strand at 1568.680 kb on + strand at 1568.680 kb on + strand at 1568.710 kb on + strand at 1568.765 kb on - strand at 1568.766 kb on + strand at 1568.771 kb on + strand at 1568.798 kb on - strand at 1568.816 kb on - strand at 1568.816 kb on - strand at 1568.816 kb on - strand at 1568.817 kb on + strand at 1568.879 kb on + strand at 1568.899 kb on - strand at 1568.900 kb on + strand at 1568.919 kb on + strand at 1568.936 kb on - strand, within MMJJ_RS08530 at 1569.026 kb on - strand, within MMJJ_RS08530 at 1569.036 kb on + strand, within MMJJ_RS08530 at 1569.148 kb on - strand, within MMJJ_RS08530 at 1569.171 kb on - strand, within MMJJ_RS08530 at 1569.171 kb on - strand, within MMJJ_RS08530 at 1569.172 kb on + strand, within MMJJ_RS08530 at 1569.272 kb on - strand, within MMJJ_RS08530 at 1569.273 kb on + strand, within MMJJ_RS08530 at 1569.279 kb on + strand, within MMJJ_RS08530 at 1569.279 kb on + strand, within MMJJ_RS08530 at 1569.322 kb on + strand, within MMJJ_RS08530 at 1569.326 kb on - strand, within MMJJ_RS08530 at 1569.327 kb on + strand, within MMJJ_RS08530 at 1569.327 kb on + strand, within MMJJ_RS08530 at 1569.327 kb on + strand, within MMJJ_RS08530 at 1569.366 kb on - strand, within MMJJ_RS08530 at 1569.366 kb on - strand, within MMJJ_RS08530 at 1569.366 kb on - strand, within MMJJ_RS08530 at 1569.396 kb on + strand, within MMJJ_RS08530 at 1569.558 kb on + strand, within MMJJ_RS08530 at 1569.569 kb on + strand, within MMJJ_RS08530 at 1569.569 kb on + strand, within MMJJ_RS08530 at 1569.622 kb on + strand, within MMJJ_RS08530 at 1569.644 kb on - strand, within MMJJ_RS08530 at 1569.650 kb on - strand, within MMJJ_RS08530 at 1569.651 kb on + strand, within MMJJ_RS08530 at 1569.651 kb on + strand, within MMJJ_RS08530 at 1569.651 kb on + strand, within MMJJ_RS08530 at 1569.655 kb on + strand, within MMJJ_RS08530 at 1569.657 kb on - strand, within MMJJ_RS08530 at 1569.657 kb on - strand, within MMJJ_RS08530 at 1569.668 kb on - strand, within MMJJ_RS08530 at 1569.737 kb on - strand, within MMJJ_RS08530 at 1569.759 kb on + strand, within MMJJ_RS08530 at 1569.762 kb on + strand, within MMJJ_RS08530 at 1569.803 kb on + strand, within MMJJ_RS08530 at 1569.819 kb on - strand, within MMJJ_RS08530 at 1569.821 kb on - strand, within MMJJ_RS08530 at 1569.884 kb on + strand, within MMJJ_RS08530 at 1569.935 kb on - strand, within MMJJ_RS08530 at 1569.963 kb on + strand, within MMJJ_RS08530 at 1570.045 kb on + strand, within MMJJ_RS08530 at 1570.237 kb on + strand, within MMJJ_RS08530 at 1570.361 kb on + strand, within MMJJ_RS08530 at 1570.392 kb on + strand, within MMJJ_RS08530 at 1570.392 kb on + strand, within MMJJ_RS08530 at 1570.473 kb on + strand, within MMJJ_RS08530 at 1570.611 kb on + strand, within MMJJ_RS08530 at 1570.637 kb on - strand at 1570.661 kb on - strand at 1570.661 kb on - strand at 1570.675 kb on + strand at 1570.675 kb on + strand at 1570.750 kb on - strand at 1570.750 kb on - strand at 1570.845 kb on - strand at 1570.849 kb on - strand at 1570.887 kb on + strand at 1570.908 kb on - strand at 1571.056 kb on + strand, within MMJJ_RS08535 at 1571.101 kb on + strand, within MMJJ_RS08535 at 1571.106 kb on + strand, within MMJJ_RS08535 at 1571.182 kb on - strand, within MMJJ_RS08535 at 1571.184 kb on - strand, within MMJJ_RS08535 at 1571.184 kb on - strand, within MMJJ_RS08535 at 1571.266 kb on - strand, within MMJJ_RS08535 at 1571.589 kb on + strand, within MMJJ_RS08535 at 1571.714 kb on - strand at 1571.718 kb on + strand at 1571.720 kb on - strand at 1571.722 kb on - strand at 1571.726 kb on + strand at 1571.726 kb on + strand at 1571.729 kb on - strand at 1571.730 kb on + strand at 1571.730 kb on + strand at 1571.732 kb on - strand at 1571.754 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete hypophosphite 62.5 mM remove 1,567,808 + MMJJ_RS08520 0.56 +0.5 1,567,835 + MMJJ_RS08520 0.62 +1.6 1,567,966 + MMJJ_RS08520 0.89 +0.5 1,568,010 + -2.8 1,568,037 - -2.8 1,568,038 + -0.3 1,568,089 + -1.0 1,568,132 + MMJJ_RS08525 0.11 -3.1 1,568,172 - MMJJ_RS08525 0.18 -0.2 1,568,173 + MMJJ_RS08525 0.18 -1.0 1,568,178 + MMJJ_RS08525 0.19 -1.5 1,568,310 - +1.0 1,568,311 + MMJJ_RS08525 0.41 +0.4 1,568,448 - MMJJ_RS08525 0.64 -1.6 1,568,449 + MMJJ_RS08525 0.64 +1.3 1,568,496 + MMJJ_RS08525 0.72 +2.3 1,568,542 - MMJJ_RS08525 0.79 +0.0 1,568,600 + MMJJ_RS08525 0.89 -0.3 1,568,634 - -2.5 1,568,635 + -3.4 1,568,650 - +3.1 1,568,680 + -0.0 1,568,680 + -1.2 1,568,710 + +1.0 1,568,765 - -0.3 1,568,766 + -3.0 1,568,771 + +1.0 1,568,798 - +2.4 1,568,816 - +1.2 1,568,816 - -0.7 1,568,816 - -0.3 1,568,817 + -0.5 1,568,879 + -1.8 1,568,899 - -0.4 1,568,900 + -0.8 1,568,919 + +0.8 1,568,936 - MMJJ_RS08530 0.11 +4.0 1,569,026 - MMJJ_RS08530 0.15 -0.4 1,569,036 + MMJJ_RS08530 0.15 -4.5 1,569,148 - MMJJ_RS08530 0.21 +0.2 1,569,171 - MMJJ_RS08530 0.22 +1.9 1,569,171 - MMJJ_RS08530 0.22 -0.5 1,569,172 + MMJJ_RS08530 0.22 +1.2 1,569,272 - MMJJ_RS08530 0.26 -0.9 1,569,273 + MMJJ_RS08530 0.26 -1.2 1,569,279 + MMJJ_RS08530 0.27 +0.3 1,569,279 + MMJJ_RS08530 0.27 -0.9 1,569,322 + MMJJ_RS08530 0.29 +0.6 1,569,326 - MMJJ_RS08530 0.29 -1.8 1,569,327 + MMJJ_RS08530 0.29 -3.0 1,569,327 + MMJJ_RS08530 0.29 -0.2 1,569,327 + MMJJ_RS08530 0.29 -0.5 1,569,366 - MMJJ_RS08530 0.31 -1.5 1,569,366 - MMJJ_RS08530 0.31 -1.3 1,569,366 - MMJJ_RS08530 0.31 -0.1 1,569,396 + MMJJ_RS08530 0.32 +0.5 1,569,558 + MMJJ_RS08530 0.40 +0.2 1,569,569 + MMJJ_RS08530 0.40 +0.7 1,569,569 + MMJJ_RS08530 0.40 +0.5 1,569,622 + MMJJ_RS08530 0.43 -0.0 1,569,644 - MMJJ_RS08530 0.44 -0.5 1,569,650 - MMJJ_RS08530 0.44 -2.3 1,569,651 + MMJJ_RS08530 0.44 -1.0 1,569,651 + MMJJ_RS08530 0.44 +0.9 1,569,651 + MMJJ_RS08530 0.44 +0.9 1,569,655 + MMJJ_RS08530 0.44 -4.9 1,569,657 - MMJJ_RS08530 0.44 -1.0 1,569,657 - MMJJ_RS08530 0.44 +0.9 1,569,668 - MMJJ_RS08530 0.45 -0.3 1,569,737 - MMJJ_RS08530 0.48 +0.3 1,569,759 + MMJJ_RS08530 0.49 -2.0 1,569,762 + MMJJ_RS08530 0.49 +1.4 1,569,803 + MMJJ_RS08530 0.51 -2.9 1,569,819 - MMJJ_RS08530 0.52 +0.8 1,569,821 - MMJJ_RS08530 0.52 +1.3 1,569,884 + MMJJ_RS08530 0.55 -2.1 1,569,935 - MMJJ_RS08530 0.57 -0.6 1,569,963 + MMJJ_RS08530 0.59 -2.9 1,570,045 + MMJJ_RS08530 0.62 -2.5 1,570,237 + MMJJ_RS08530 0.71 +0.8 1,570,361 + MMJJ_RS08530 0.77 -3.8 1,570,392 + MMJJ_RS08530 0.79 +0.4 1,570,392 + MMJJ_RS08530 0.79 -3.0 1,570,473 + MMJJ_RS08530 0.82 -2.6 1,570,611 + MMJJ_RS08530 0.89 +0.2 1,570,637 - +0.9 1,570,661 - -0.0 1,570,661 - -1.1 1,570,675 + +2.1 1,570,675 + -1.1 1,570,750 - -0.3 1,570,750 - -2.2 1,570,845 - -3.0 1,570,849 - +1.5 1,570,887 + -3.1 1,570,908 - -0.7 1,571,056 + MMJJ_RS08535 0.20 +1.0 1,571,101 + MMJJ_RS08535 0.25 -1.9 1,571,106 + MMJJ_RS08535 0.26 -1.7 1,571,182 - MMJJ_RS08535 0.35 -2.8 1,571,184 - MMJJ_RS08535 0.36 -1.5 1,571,184 - MMJJ_RS08535 0.36 -2.8 1,571,266 - MMJJ_RS08535 0.46 -2.2 1,571,589 + MMJJ_RS08535 0.86 -3.5 1,571,714 - -0.8 1,571,718 + -0.6 1,571,720 - -1.8 1,571,722 - -1.1 1,571,726 + -1.0 1,571,726 + -1.3 1,571,729 - +2.0 1,571,730 + -1.6 1,571,730 + -2.0 1,571,732 - +1.6 1,571,754 + +1.3
Or see this region's nucleotide sequence