Experiment: McCas_formate_complete hypophosphite 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06585 and MMJJ_RS06590 are separated by 223 nucleotides MMJJ_RS06590 and MMJJ_RS06595 are separated by 179 nucleotides
MMJJ_RS06585: MMJJ_RS06585 - alanine racemase, at 1,216,124 to 1,217,245
_RS06585
MMJJ_RS06590: MMJJ_RS06590 - sodium:alanine symporter family protein, at 1,217,469 to 1,218,830
_RS06590
MMJJ_RS06595: MMJJ_RS06595 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA, at 1,219,010 to 1,220,305
_RS06595
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1216.514 kb on + strand, within MMJJ_RS06585 at 1216.642 kb on - strand, within MMJJ_RS06585 at 1216.649 kb on - strand, within MMJJ_RS06585 at 1216.649 kb on - strand, within MMJJ_RS06585 at 1216.650 kb on + strand, within MMJJ_RS06585 at 1216.650 kb on + strand, within MMJJ_RS06585 at 1216.658 kb on + strand at 1216.669 kb on - strand, within MMJJ_RS06585 at 1216.739 kb on + strand, within MMJJ_RS06585 at 1216.749 kb on - strand, within MMJJ_RS06585 at 1216.777 kb on - strand, within MMJJ_RS06585 at 1216.796 kb on + strand, within MMJJ_RS06585 at 1216.843 kb on - strand, within MMJJ_RS06585 at 1216.872 kb on - strand, within MMJJ_RS06585 at 1216.873 kb on + strand, within MMJJ_RS06585 at 1216.873 kb on + strand, within MMJJ_RS06585 at 1216.873 kb on + strand, within MMJJ_RS06585 at 1216.878 kb on - strand, within MMJJ_RS06585 at 1216.878 kb on - strand, within MMJJ_RS06585 at 1216.879 kb on + strand, within MMJJ_RS06585 at 1216.887 kb on - strand, within MMJJ_RS06585 at 1216.887 kb on - strand, within MMJJ_RS06585 at 1216.888 kb on + strand, within MMJJ_RS06585 at 1216.888 kb on + strand, within MMJJ_RS06585 at 1216.920 kb on + strand, within MMJJ_RS06585 at 1216.920 kb on + strand, within MMJJ_RS06585 at 1216.920 kb on + strand, within MMJJ_RS06585 at 1216.976 kb on - strand, within MMJJ_RS06585 at 1217.083 kb on - strand, within MMJJ_RS06585 at 1217.084 kb on + strand, within MMJJ_RS06585 at 1217.090 kb on + strand, within MMJJ_RS06585 at 1217.100 kb on - strand, within MMJJ_RS06585 at 1217.170 kb on - strand at 1217.188 kb on + strand at 1217.188 kb on + strand at 1217.211 kb on - strand at 1217.211 kb on - strand at 1217.232 kb on - strand at 1217.233 kb on + strand at 1217.233 kb on + strand at 1217.234 kb on - strand at 1217.234 kb on - strand at 1217.234 kb on - strand at 1217.234 kb on - strand at 1217.234 kb on - strand at 1217.234 kb on - strand at 1217.235 kb on + strand at 1217.235 kb on + strand at 1217.238 kb on - strand at 1217.238 kb on - strand at 1217.243 kb on - strand at 1217.243 kb on - strand at 1217.244 kb on + strand at 1217.301 kb on - strand at 1217.306 kb on + strand at 1217.306 kb on + strand at 1217.308 kb on - strand at 1217.308 kb on - strand at 1217.308 kb on - strand at 1217.308 kb on - strand at 1217.309 kb on + strand at 1217.309 kb on + strand at 1217.309 kb on + strand at 1217.314 kb on + strand at 1217.316 kb on + strand at 1217.322 kb on - strand at 1217.322 kb on - strand at 1217.323 kb on + strand at 1217.323 kb on + strand at 1217.323 kb on + strand at 1217.325 kb on + strand at 1217.357 kb on - strand at 1217.377 kb on - strand at 1217.377 kb on - strand at 1217.379 kb on - strand at 1217.379 kb on - strand at 1217.379 kb on - strand at 1217.379 kb on - strand at 1217.379 kb on - strand at 1217.379 kb on - strand at 1217.379 kb on - strand at 1217.380 kb on + strand at 1217.380 kb on + strand at 1217.380 kb on + strand at 1217.380 kb on + strand at 1217.380 kb on + strand at 1217.380 kb on + strand at 1217.395 kb on - strand at 1217.396 kb on + strand at 1217.422 kb on - strand at 1217.423 kb on + strand at 1217.429 kb on - strand at 1217.493 kb on + strand at 1217.498 kb on - strand at 1217.499 kb on + strand at 1217.520 kb on - strand at 1217.520 kb on - strand at 1217.520 kb on - strand at 1217.520 kb on - strand at 1217.520 kb on - strand at 1217.520 kb on - strand at 1217.520 kb on - strand at 1217.521 kb on + strand at 1217.521 kb on + strand at 1217.530 kb on - strand at 1217.558 kb on + strand at 1217.558 kb on + strand at 1217.569 kb on - strand at 1217.605 kb on - strand at 1217.605 kb on - strand at 1217.730 kb on - strand, within MMJJ_RS06590 at 1217.730 kb on - strand, within MMJJ_RS06590 at 1217.731 kb on + strand, within MMJJ_RS06590 at 1217.731 kb on + strand, within MMJJ_RS06590 at 1217.731 kb on + strand at 1217.731 kb on + strand, within MMJJ_RS06590 at 1217.800 kb on + strand, within MMJJ_RS06590 at 1217.800 kb on + strand, within MMJJ_RS06590 at 1217.800 kb on + strand, within MMJJ_RS06590 at 1217.822 kb on - strand, within MMJJ_RS06590 at 1217.823 kb on + strand, within MMJJ_RS06590 at 1217.826 kb on - strand, within MMJJ_RS06590 at 1217.826 kb on - strand, within MMJJ_RS06590 at 1217.827 kb on + strand, within MMJJ_RS06590 at 1217.871 kb on - strand, within MMJJ_RS06590 at 1217.872 kb on + strand, within MMJJ_RS06590 at 1217.872 kb on + strand, within MMJJ_RS06590 at 1217.872 kb on + strand, within MMJJ_RS06590 at 1217.872 kb on + strand, within MMJJ_RS06590 at 1217.967 kb on + strand, within MMJJ_RS06590 at 1218.028 kb on - strand, within MMJJ_RS06590 at 1218.028 kb on - strand, within MMJJ_RS06590 at 1218.032 kb on - strand, within MMJJ_RS06590 at 1218.068 kb on - strand, within MMJJ_RS06590 at 1218.069 kb on + strand, within MMJJ_RS06590 at 1218.083 kb on - strand, within MMJJ_RS06590 at 1218.083 kb on - strand, within MMJJ_RS06590 at 1218.084 kb on + strand, within MMJJ_RS06590 at 1218.101 kb on - strand, within MMJJ_RS06590 at 1218.107 kb on - strand, within MMJJ_RS06590 at 1218.139 kb on - strand, within MMJJ_RS06590 at 1218.140 kb on + strand, within MMJJ_RS06590 at 1218.171 kb on + strand, within MMJJ_RS06590 at 1218.182 kb on - strand, within MMJJ_RS06590 at 1218.203 kb on + strand, within MMJJ_RS06590 at 1218.203 kb on + strand, within MMJJ_RS06590 at 1218.244 kb on - strand, within MMJJ_RS06590 at 1218.244 kb on - strand, within MMJJ_RS06590 at 1218.245 kb on + strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.403 kb on - strand, within MMJJ_RS06590 at 1218.404 kb on + strand, within MMJJ_RS06590 at 1218.404 kb on + strand, within MMJJ_RS06590 at 1218.408 kb on + strand, within MMJJ_RS06590 at 1218.408 kb on + strand, within MMJJ_RS06590 at 1218.508 kb on - strand, within MMJJ_RS06590 at 1218.547 kb on - strand, within MMJJ_RS06590 at 1218.568 kb on - strand, within MMJJ_RS06590 at 1218.585 kb on - strand, within MMJJ_RS06590 at 1218.629 kb on - strand, within MMJJ_RS06590 at 1218.637 kb on + strand, within MMJJ_RS06590 at 1218.655 kb on - strand, within MMJJ_RS06590 at 1218.655 kb on - strand, within MMJJ_RS06590 at 1218.694 kb on - strand at 1218.695 kb on + strand at 1218.710 kb on - strand at 1218.711 kb on + strand at 1218.769 kb on - strand at 1218.819 kb on + strand at 1218.852 kb on + strand at 1218.879 kb on + strand at 1218.884 kb on + strand at 1218.924 kb on - strand at 1218.925 kb on + strand at 1218.926 kb on - strand at 1218.926 kb on - strand at 1218.927 kb on + strand at 1218.932 kb on - strand at 1218.933 kb on + strand at 1218.940 kb on - strand at 1218.940 kb on - strand at 1218.943 kb on - strand at 1218.943 kb on - strand at 1218.944 kb on + strand at 1219.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete hypophosphite 62.5 mM remove 1,216,514 + MMJJ_RS06585 0.35 +0.7 1,216,642 - MMJJ_RS06585 0.46 -3.5 1,216,649 - MMJJ_RS06585 0.47 +1.3 1,216,649 - MMJJ_RS06585 0.47 -0.9 1,216,650 + MMJJ_RS06585 0.47 -1.0 1,216,650 + MMJJ_RS06585 0.47 -1.1 1,216,658 + +0.6 1,216,669 - MMJJ_RS06585 0.49 +2.1 1,216,739 + MMJJ_RS06585 0.55 +0.4 1,216,749 - MMJJ_RS06585 0.56 +1.9 1,216,777 - MMJJ_RS06585 0.58 +0.9 1,216,796 + MMJJ_RS06585 0.60 +0.8 1,216,843 - MMJJ_RS06585 0.64 +0.8 1,216,872 - MMJJ_RS06585 0.67 +4.1 1,216,873 + MMJJ_RS06585 0.67 +0.9 1,216,873 + MMJJ_RS06585 0.67 +0.5 1,216,873 + MMJJ_RS06585 0.67 +0.8 1,216,878 - MMJJ_RS06585 0.67 +1.1 1,216,878 - MMJJ_RS06585 0.67 -0.2 1,216,879 + MMJJ_RS06585 0.67 +0.8 1,216,887 - MMJJ_RS06585 0.68 -1.4 1,216,887 - MMJJ_RS06585 0.68 -0.3 1,216,888 + MMJJ_RS06585 0.68 -2.9 1,216,888 + MMJJ_RS06585 0.68 +0.4 1,216,920 + MMJJ_RS06585 0.71 +0.7 1,216,920 + MMJJ_RS06585 0.71 +0.2 1,216,920 + MMJJ_RS06585 0.71 -0.7 1,216,976 - MMJJ_RS06585 0.76 -1.1 1,217,083 - MMJJ_RS06585 0.85 +1.6 1,217,084 + MMJJ_RS06585 0.86 +0.9 1,217,090 + MMJJ_RS06585 0.86 -1.0 1,217,100 - MMJJ_RS06585 0.87 +0.1 1,217,170 - +0.1 1,217,188 + -0.0 1,217,188 + -0.9 1,217,211 - +2.5 1,217,211 - +1.8 1,217,232 - -2.3 1,217,233 + +1.0 1,217,233 + +2.3 1,217,234 - +1.5 1,217,234 - -3.7 1,217,234 - +0.3 1,217,234 - -1.8 1,217,234 - +1.4 1,217,234 - +0.1 1,217,235 + +2.0 1,217,235 + +1.6 1,217,238 - -0.9 1,217,238 - +0.2 1,217,243 - -0.5 1,217,243 - -0.3 1,217,244 + -1.8 1,217,301 - +2.0 1,217,306 + -0.2 1,217,306 + +0.2 1,217,308 - -2.2 1,217,308 - +2.8 1,217,308 - +0.7 1,217,308 - -0.8 1,217,309 + +0.7 1,217,309 + -0.2 1,217,309 + -1.3 1,217,314 + +0.3 1,217,316 + -1.2 1,217,322 - +0.7 1,217,322 - +2.0 1,217,323 + -0.9 1,217,323 + +0.1 1,217,323 + +0.5 1,217,325 + +2.5 1,217,357 - +0.8 1,217,377 - -2.6 1,217,377 - -0.1 1,217,379 - -1.2 1,217,379 - +0.9 1,217,379 - -1.2 1,217,379 - -0.8 1,217,379 - -0.1 1,217,379 - -0.7 1,217,379 - -3.9 1,217,380 + -1.0 1,217,380 + -0.1 1,217,380 + -0.3 1,217,380 + -2.0 1,217,380 + +0.1 1,217,380 + +0.2 1,217,395 - -2.3 1,217,396 + +0.5 1,217,422 - -1.8 1,217,423 + -0.4 1,217,429 - -2.3 1,217,493 + +1.5 1,217,498 - -1.1 1,217,499 + +0.8 1,217,520 - -4.3 1,217,520 - +0.6 1,217,520 - -2.0 1,217,520 - +0.2 1,217,520 - -0.8 1,217,520 - -0.1 1,217,520 - -1.4 1,217,521 + -2.5 1,217,521 + +0.1 1,217,530 - -1.6 1,217,558 + -1.7 1,217,558 + -4.0 1,217,569 - -0.3 1,217,605 - -2.9 1,217,605 - -1.7 1,217,730 - MMJJ_RS06590 0.19 -1.4 1,217,730 - MMJJ_RS06590 0.19 -0.5 1,217,731 + MMJJ_RS06590 0.19 -0.4 1,217,731 + MMJJ_RS06590 0.19 -1.4 1,217,731 + +2.2 1,217,731 + MMJJ_RS06590 0.19 -0.4 1,217,800 + MMJJ_RS06590 0.24 +0.7 1,217,800 + MMJJ_RS06590 0.24 -3.2 1,217,800 + MMJJ_RS06590 0.24 -0.3 1,217,822 - MMJJ_RS06590 0.26 -1.7 1,217,823 + MMJJ_RS06590 0.26 -1.8 1,217,826 - MMJJ_RS06590 0.26 -1.8 1,217,826 - MMJJ_RS06590 0.26 -4.5 1,217,827 + MMJJ_RS06590 0.26 +0.2 1,217,871 - MMJJ_RS06590 0.30 -1.3 1,217,872 + MMJJ_RS06590 0.30 +0.7 1,217,872 + MMJJ_RS06590 0.30 -0.8 1,217,872 + MMJJ_RS06590 0.30 -1.5 1,217,872 + MMJJ_RS06590 0.30 -1.7 1,217,967 + MMJJ_RS06590 0.37 -1.2 1,218,028 - MMJJ_RS06590 0.41 -1.8 1,218,028 - MMJJ_RS06590 0.41 -0.2 1,218,032 - MMJJ_RS06590 0.41 -1.8 1,218,068 - MMJJ_RS06590 0.44 -0.5 1,218,069 + MMJJ_RS06590 0.44 -2.6 1,218,083 - MMJJ_RS06590 0.45 -1.6 1,218,083 - MMJJ_RS06590 0.45 -3.4 1,218,084 + MMJJ_RS06590 0.45 +0.7 1,218,101 - MMJJ_RS06590 0.46 -2.8 1,218,107 - MMJJ_RS06590 0.47 -0.5 1,218,139 - MMJJ_RS06590 0.49 -3.7 1,218,140 + MMJJ_RS06590 0.49 +1.2 1,218,171 + MMJJ_RS06590 0.52 -2.0 1,218,182 - MMJJ_RS06590 0.52 -2.7 1,218,203 + MMJJ_RS06590 0.54 -0.4 1,218,203 + MMJJ_RS06590 0.54 -2.5 1,218,244 - MMJJ_RS06590 0.57 -1.3 1,218,244 - MMJJ_RS06590 0.57 -0.2 1,218,245 + MMJJ_RS06590 0.57 -2.3 1,218,403 - MMJJ_RS06590 0.69 -1.8 1,218,403 - MMJJ_RS06590 0.69 -2.4 1,218,403 - MMJJ_RS06590 0.69 -1.6 1,218,403 - MMJJ_RS06590 0.69 -3.7 1,218,404 + MMJJ_RS06590 0.69 -4.1 1,218,404 + MMJJ_RS06590 0.69 -0.6 1,218,408 + MMJJ_RS06590 0.69 -3.4 1,218,408 + MMJJ_RS06590 0.69 -1.0 1,218,508 - MMJJ_RS06590 0.76 +0.1 1,218,547 - MMJJ_RS06590 0.79 -4.2 1,218,568 - MMJJ_RS06590 0.81 -0.7 1,218,585 - MMJJ_RS06590 0.82 -1.3 1,218,629 - MMJJ_RS06590 0.85 -1.9 1,218,637 + MMJJ_RS06590 0.86 -3.6 1,218,655 - MMJJ_RS06590 0.87 -3.3 1,218,655 - MMJJ_RS06590 0.87 -4.5 1,218,694 - -2.1 1,218,695 + -2.5 1,218,710 - +0.4 1,218,711 + -1.3 1,218,769 - -2.9 1,218,819 + -0.1 1,218,852 + +0.8 1,218,879 + +0.8 1,218,884 + -2.6 1,218,924 - -0.5 1,218,925 + -0.1 1,218,926 - +0.5 1,218,926 - +0.5 1,218,927 + -0.7 1,218,932 - -1.9 1,218,933 + +1.1 1,218,940 - -0.1 1,218,940 - +0.6 1,218,943 - +0.8 1,218,943 - -3.0 1,218,944 + +0.7 1,219,008 + +0.2
Or see this region's nucleotide sequence