Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06815 and MMJJ_RS06820 are separated by 19 nucleotides MMJJ_RS06820 and MMJJ_RS06825 are separated by 15 nucleotides MMJJ_RS06825 and MMJJ_RS06830 are separated by 20 nucleotides MMJJ_RS06830 and MMJJ_RS06835 overlap by 8 nucleotides
MMJJ_RS06815: MMJJ_RS06815 - HTH domain-containing protein, at 1,259,049 to 1,259,444
_RS06815
MMJJ_RS06820: MMJJ_RS06820 - CBS domain-containing protein, at 1,259,464 to 1,260,105
_RS06820
MMJJ_RS06825: MMJJ_RS06825 - 4Fe-4S binding protein, at 1,260,121 to 1,260,879
_RS06825
MMJJ_RS06830: MMJJ_RS06830 - 4Fe-4S binding protein, at 1,260,900 to 1,261,385
_RS06830
MMJJ_RS06835: MMJJ_RS06835 - 4Fe-4S binding protein, at 1,261,378 to 1,262,559
_RS06835
Position (kb)
1260
1261 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1259.283 kb on + strand, within MMJJ_RS06815 at 1259.426 kb on - strand at 1259.491 kb on - strand at 1259.492 kb on + strand at 1259.583 kb on - strand, within MMJJ_RS06820 at 1259.584 kb on + strand, within MMJJ_RS06820 at 1259.593 kb on - strand, within MMJJ_RS06820 at 1259.594 kb on + strand, within MMJJ_RS06820 at 1259.712 kb on - strand, within MMJJ_RS06820 at 1259.741 kb on + strand, within MMJJ_RS06820 at 1259.764 kb on + strand, within MMJJ_RS06820 at 1259.822 kb on + strand, within MMJJ_RS06820 at 1260.052 kb on + strand at 1260.064 kb on - strand at 1260.108 kb on - strand at 1260.188 kb on + strand at 1260.188 kb on + strand at 1260.199 kb on + strand, within MMJJ_RS06825 at 1260.233 kb on + strand, within MMJJ_RS06825 at 1260.251 kb on + strand, within MMJJ_RS06825 at 1260.268 kb on + strand, within MMJJ_RS06825 at 1260.288 kb on - strand, within MMJJ_RS06825 at 1260.288 kb on - strand, within MMJJ_RS06825 at 1260.292 kb on - strand, within MMJJ_RS06825 at 1260.298 kb on - strand, within MMJJ_RS06825 at 1260.299 kb on + strand, within MMJJ_RS06825 at 1260.317 kb on + strand, within MMJJ_RS06825 at 1260.361 kb on - strand, within MMJJ_RS06825 at 1260.362 kb on + strand, within MMJJ_RS06825 at 1260.362 kb on + strand, within MMJJ_RS06825 at 1260.378 kb on - strand, within MMJJ_RS06825 at 1260.379 kb on + strand, within MMJJ_RS06825 at 1260.398 kb on + strand, within MMJJ_RS06825 at 1260.403 kb on - strand, within MMJJ_RS06825 at 1260.403 kb on - strand, within MMJJ_RS06825 at 1260.404 kb on + strand, within MMJJ_RS06825 at 1260.404 kb on + strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.475 kb on - strand, within MMJJ_RS06825 at 1260.505 kb on - strand, within MMJJ_RS06825 at 1260.505 kb on - strand, within MMJJ_RS06825 at 1260.515 kb on + strand, within MMJJ_RS06825 at 1260.529 kb on - strand, within MMJJ_RS06825 at 1260.530 kb on + strand, within MMJJ_RS06825 at 1260.556 kb on - strand, within MMJJ_RS06825 at 1260.557 kb on + strand, within MMJJ_RS06825 at 1260.590 kb on + strand, within MMJJ_RS06825 at 1260.732 kb on - strand, within MMJJ_RS06825 at 1260.733 kb on + strand, within MMJJ_RS06825 at 1260.733 kb on + strand, within MMJJ_RS06825 at 1260.751 kb on - strand, within MMJJ_RS06825 at 1260.751 kb on - strand, within MMJJ_RS06825 at 1260.837 kb on - strand at 1260.874 kb on - strand at 1260.898 kb on - strand at 1260.898 kb on - strand at 1260.902 kb on + strand at 1260.931 kb on + strand at 1260.980 kb on - strand, within MMJJ_RS06830 at 1260.981 kb on + strand, within MMJJ_RS06830 at 1261.025 kb on - strand, within MMJJ_RS06830 at 1261.029 kb on - strand, within MMJJ_RS06830 at 1261.061 kb on + strand, within MMJJ_RS06830 at 1261.061 kb on + strand, within MMJJ_RS06830 at 1261.124 kb on - strand, within MMJJ_RS06830 at 1261.130 kb on - strand, within MMJJ_RS06830 at 1261.191 kb on + strand, within MMJJ_RS06830 at 1261.191 kb on + strand, within MMJJ_RS06830 at 1261.259 kb on - strand, within MMJJ_RS06830 at 1261.260 kb on + strand, within MMJJ_RS06830 at 1261.260 kb on + strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.309 kb on - strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.310 kb on + strand, within MMJJ_RS06830 at 1261.352 kb on + strand at 1261.440 kb on - strand at 1261.454 kb on + strand at 1261.454 kb on + strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.459 kb on - strand at 1261.460 kb on + strand at 1261.460 kb on + strand at 1261.460 kb on + strand at 1261.520 kb on + strand, within MMJJ_RS06835 at 1261.535 kb on - strand, within MMJJ_RS06835 at 1261.561 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.601 kb on + strand, within MMJJ_RS06835 at 1261.612 kb on - strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.613 kb on + strand, within MMJJ_RS06835 at 1261.620 kb on + strand, within MMJJ_RS06835 at 1261.635 kb on + strand, within MMJJ_RS06835 at 1261.661 kb on + strand, within MMJJ_RS06835 at 1261.680 kb on - strand, within MMJJ_RS06835 at 1261.681 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.692 kb on + strand, within MMJJ_RS06835 at 1261.734 kb on + strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.822 kb on - strand, within MMJJ_RS06835 at 1261.823 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.840 kb on + strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835 at 1261.855 kb on - strand, within MMJJ_RS06835
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 1,259,283 + MMJJ_RS06815 0.59 -1.4 1,259,426 - +0.1 1,259,491 - -2.7 1,259,492 + -2.2 1,259,583 - MMJJ_RS06820 0.19 +0.4 1,259,584 + MMJJ_RS06820 0.19 +0.1 1,259,593 - MMJJ_RS06820 0.20 +1.1 1,259,594 + MMJJ_RS06820 0.20 -0.6 1,259,712 - MMJJ_RS06820 0.39 +0.5 1,259,741 + MMJJ_RS06820 0.43 -0.2 1,259,764 + MMJJ_RS06820 0.47 -4.0 1,259,822 + MMJJ_RS06820 0.56 +1.0 1,260,052 + +0.2 1,260,064 - +0.1 1,260,108 - +0.7 1,260,188 + -1.7 1,260,188 + -2.3 1,260,199 + MMJJ_RS06825 0.10 -2.3 1,260,233 + MMJJ_RS06825 0.15 -1.0 1,260,251 + MMJJ_RS06825 0.17 -0.7 1,260,268 + MMJJ_RS06825 0.19 -1.3 1,260,288 - MMJJ_RS06825 0.22 -3.8 1,260,288 - MMJJ_RS06825 0.22 -2.2 1,260,292 - MMJJ_RS06825 0.23 -1.6 1,260,298 - MMJJ_RS06825 0.23 -2.1 1,260,299 + MMJJ_RS06825 0.23 -1.1 1,260,317 + MMJJ_RS06825 0.26 -1.8 1,260,361 - MMJJ_RS06825 0.32 +0.2 1,260,362 + MMJJ_RS06825 0.32 -2.1 1,260,362 + MMJJ_RS06825 0.32 -1.4 1,260,378 - MMJJ_RS06825 0.34 +0.2 1,260,379 + MMJJ_RS06825 0.34 -0.9 1,260,398 + MMJJ_RS06825 0.36 -0.5 1,260,403 - MMJJ_RS06825 0.37 -1.0 1,260,403 - MMJJ_RS06825 0.37 -2.4 1,260,404 + MMJJ_RS06825 0.37 -0.7 1,260,404 + MMJJ_RS06825 0.37 -0.7 1,260,475 - MMJJ_RS06825 0.47 -0.2 1,260,475 - MMJJ_RS06825 0.47 -1.3 1,260,475 - MMJJ_RS06825 0.47 +0.3 1,260,505 - MMJJ_RS06825 0.51 -0.0 1,260,505 - MMJJ_RS06825 0.51 +0.3 1,260,515 + MMJJ_RS06825 0.52 -0.6 1,260,529 - MMJJ_RS06825 0.54 -2.1 1,260,530 + MMJJ_RS06825 0.54 -1.6 1,260,556 - MMJJ_RS06825 0.57 -0.8 1,260,557 + MMJJ_RS06825 0.57 -1.9 1,260,590 + MMJJ_RS06825 0.62 -1.6 1,260,732 - MMJJ_RS06825 0.81 -0.6 1,260,733 + MMJJ_RS06825 0.81 +2.5 1,260,733 + MMJJ_RS06825 0.81 -0.3 1,260,751 - MMJJ_RS06825 0.83 -3.5 1,260,751 - MMJJ_RS06825 0.83 -2.8 1,260,837 - +0.3 1,260,874 - -2.1 1,260,898 - -0.6 1,260,898 - +1.1 1,260,902 + +0.2 1,260,931 + +0.4 1,260,980 - MMJJ_RS06830 0.16 -1.0 1,260,981 + MMJJ_RS06830 0.17 -1.9 1,261,025 - MMJJ_RS06830 0.26 -1.0 1,261,029 - MMJJ_RS06830 0.27 +1.0 1,261,061 + MMJJ_RS06830 0.33 +0.2 1,261,061 + MMJJ_RS06830 0.33 +0.1 1,261,124 - MMJJ_RS06830 0.46 -3.7 1,261,130 - MMJJ_RS06830 0.47 -2.4 1,261,191 + MMJJ_RS06830 0.60 -1.5 1,261,191 + MMJJ_RS06830 0.60 +0.3 1,261,259 - MMJJ_RS06830 0.74 -1.5 1,261,260 + MMJJ_RS06830 0.74 +0.2 1,261,260 + MMJJ_RS06830 0.74 -0.4 1,261,309 - MMJJ_RS06830 0.84 -0.1 1,261,309 - MMJJ_RS06830 0.84 -1.4 1,261,309 - MMJJ_RS06830 0.84 +0.3 1,261,310 + MMJJ_RS06830 0.84 -1.3 1,261,310 + MMJJ_RS06830 0.84 +1.0 1,261,310 + MMJJ_RS06830 0.84 +0.9 1,261,352 + -2.4 1,261,440 - -1.1 1,261,454 + -1.1 1,261,454 + -1.6 1,261,459 - +0.4 1,261,459 - -0.8 1,261,459 - +0.7 1,261,459 - -0.7 1,261,459 - -1.5 1,261,460 + -2.7 1,261,460 + -2.1 1,261,460 + -1.2 1,261,520 + MMJJ_RS06835 0.12 +0.7 1,261,535 - MMJJ_RS06835 0.13 -1.7 1,261,561 + MMJJ_RS06835 0.15 -2.8 1,261,601 + MMJJ_RS06835 0.19 -1.3 1,261,601 + MMJJ_RS06835 0.19 -2.6 1,261,612 - MMJJ_RS06835 0.20 -1.5 1,261,613 + MMJJ_RS06835 0.20 +0.5 1,261,613 + MMJJ_RS06835 0.20 -2.5 1,261,620 + MMJJ_RS06835 0.20 -3.3 1,261,635 + MMJJ_RS06835 0.22 -0.9 1,261,661 + MMJJ_RS06835 0.24 -2.6 1,261,680 - MMJJ_RS06835 0.26 +1.4 1,261,681 + MMJJ_RS06835 0.26 -2.3 1,261,692 + MMJJ_RS06835 0.27 -2.7 1,261,692 + MMJJ_RS06835 0.27 -1.8 1,261,734 + MMJJ_RS06835 0.30 -0.5 1,261,822 - MMJJ_RS06835 0.38 -1.5 1,261,822 - MMJJ_RS06835 0.38 -3.1 1,261,823 + MMJJ_RS06835 0.38 -3.6 1,261,840 + MMJJ_RS06835 0.39 +0.5 1,261,840 + MMJJ_RS06835 0.39 -3.7 1,261,855 - MMJJ_RS06835 0.40 -2.3 1,261,855 - MMJJ_RS06835 0.40 -1.2
Or see this region's nucleotide sequence