Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS06425

Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS06415 and MMJJ_RS06420 are separated by 21 nucleotidesMMJJ_RS06420 and MMJJ_RS06425 are separated by 30 nucleotidesMMJJ_RS06425 and MMJJ_RS06430 are separated by 268 nucleotidesMMJJ_RS06430 and MMJJ_RS06435 are separated by 60 nucleotides MMJJ_RS06415: MMJJ_RS06415 - 50S ribosomal protein L23, at 1,186,795 to 1,187,055 _RS06415 MMJJ_RS06420: MMJJ_RS06420 - 50S ribosomal protein L4, at 1,187,077 to 1,187,835 _RS06420 MMJJ_RS06425: MMJJ_RS06425 - 50S ribosomal protein L3, at 1,187,866 to 1,188,870 _RS06425 MMJJ_RS06430: MMJJ_RS06430 - haloacid dehalogenase, at 1,189,139 to 1,189,729 _RS06430 MMJJ_RS06435: MMJJ_RS06435 - tRNA-Ile, at 1,189,790 to 1,189,864 _RS06435 Position (kb) 1187 1188 1189Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1187.500 kb on - strandat 1189.011 kb on + strandat 1189.041 kb on - strandat 1189.050 kb on + strandat 1189.065 kb on - strandat 1189.074 kb on + strandat 1189.099 kb on - strandat 1189.104 kb on + strandat 1189.115 kb on - strandat 1189.115 kb on - strandat 1189.116 kb on + strandat 1189.154 kb on - strandat 1189.158 kb on - strandat 1189.240 kb on + strand, within MMJJ_RS06430at 1189.370 kb on - strand, within MMJJ_RS06430at 1189.371 kb on + strand, within MMJJ_RS06430at 1189.371 kb on + strand, within MMJJ_RS06430at 1189.371 kb on + strand, within MMJJ_RS06430at 1189.375 kb on + strand, within MMJJ_RS06430at 1189.375 kb on + strand, within MMJJ_RS06430at 1189.388 kb on - strand, within MMJJ_RS06430at 1189.388 kb on - strand, within MMJJ_RS06430at 1189.391 kb on - strand, within MMJJ_RS06430at 1189.446 kb on - strand, within MMJJ_RS06430at 1189.447 kb on + strand, within MMJJ_RS06430at 1189.458 kb on + strand, within MMJJ_RS06430at 1189.460 kb on + strand, within MMJJ_RS06430at 1189.493 kb on + strand, within MMJJ_RS06430at 1189.493 kb on + strand, within MMJJ_RS06430at 1189.501 kb on + strand, within MMJJ_RS06430at 1189.557 kb on + strand, within MMJJ_RS06430at 1189.558 kb on - strand, within MMJJ_RS06430at 1189.558 kb on - strand, within MMJJ_RS06430at 1189.558 kb on - strand, within MMJJ_RS06430at 1189.559 kb on + strand, within MMJJ_RS06430at 1189.559 kb on + strand, within MMJJ_RS06430at 1189.599 kb on + strand, within MMJJ_RS06430at 1189.618 kb on - strand, within MMJJ_RS06430at 1189.671 kb on - strandat 1189.671 kb on - strandat 1189.671 kb on - strandat 1189.671 kb on - strandat 1189.671 kb on - strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.672 kb on + strandat 1189.675 kb on - strandat 1189.675 kb on - strandat 1189.700 kb on + strandat 1189.700 kb on + strandat 1189.700 kb on + strandat 1189.700 kb on + strandat 1189.704 kb on - strandat 1189.704 kb on - strandat 1189.704 kb on - strandat 1189.704 kb on - strandat 1189.704 kb on - strandat 1189.705 kb on + strandat 1189.705 kb on + strandat 1189.727 kb on - strandat 1189.728 kb on + strandat 1189.728 kb on + strandat 1189.748 kb on + strandat 1189.753 kb on - strandat 1189.780 kb on - strandat 1189.781 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM
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1,187,500 - +0.5
1,189,011 + +0.9
1,189,041 - -1.7
1,189,050 + +0.5
1,189,065 - +0.2
1,189,074 + -0.7
1,189,099 - -1.7
1,189,104 + +0.1
1,189,115 - +1.6
1,189,115 - -1.1
1,189,116 + +1.4
1,189,154 - -0.1
1,189,158 - +0.9
1,189,240 + MMJJ_RS06430 0.17 -0.7
1,189,370 - MMJJ_RS06430 0.39 +0.0
1,189,371 + MMJJ_RS06430 0.39 +1.7
1,189,371 + MMJJ_RS06430 0.39 +2.4
1,189,371 + MMJJ_RS06430 0.39 +1.1
1,189,375 + MMJJ_RS06430 0.40 +3.3
1,189,375 + MMJJ_RS06430 0.40 -2.3
1,189,388 - MMJJ_RS06430 0.42 +1.3
1,189,388 - MMJJ_RS06430 0.42 +1.3
1,189,391 - MMJJ_RS06430 0.43 +2.2
1,189,446 - MMJJ_RS06430 0.52 -3.2
1,189,447 + MMJJ_RS06430 0.52 -0.6
1,189,458 + MMJJ_RS06430 0.54 -0.7
1,189,460 + MMJJ_RS06430 0.54 +0.4
1,189,493 + MMJJ_RS06430 0.60 -0.1
1,189,493 + MMJJ_RS06430 0.60 -1.4
1,189,501 + MMJJ_RS06430 0.61 +0.1
1,189,557 + MMJJ_RS06430 0.71 +0.5
1,189,558 - MMJJ_RS06430 0.71 -0.5
1,189,558 - MMJJ_RS06430 0.71 +0.5
1,189,558 - MMJJ_RS06430 0.71 +0.9
1,189,559 + MMJJ_RS06430 0.71 +2.4
1,189,559 + MMJJ_RS06430 0.71 +2.5
1,189,599 + MMJJ_RS06430 0.78 -0.3
1,189,618 - MMJJ_RS06430 0.81 -1.2
1,189,671 - +1.3
1,189,671 - +0.9
1,189,671 - +1.3
1,189,671 - +1.7
1,189,671 - -0.0
1,189,672 + +3.8
1,189,672 + +2.4
1,189,672 + +0.2
1,189,672 + -0.6
1,189,672 + -0.1
1,189,672 + +2.5
1,189,672 + +1.0
1,189,672 + +1.1
1,189,672 + -2.4
1,189,672 + +1.2
1,189,672 + +1.3
1,189,675 - -0.2
1,189,675 - +1.1
1,189,700 + +1.8
1,189,700 + -0.3
1,189,700 + -0.3
1,189,700 + -1.8
1,189,704 - +1.4
1,189,704 - -0.9
1,189,704 - -0.3
1,189,704 - +2.7
1,189,704 - -0.6
1,189,705 + -0.7
1,189,705 + -2.0
1,189,727 - -1.2
1,189,728 + +1.6
1,189,728 + +0.3
1,189,748 + +3.3
1,189,753 - +1.6
1,189,780 - +1.3
1,189,781 + +2.6

Or see this region's nucleotide sequence