Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06415 and MMJJ_RS06420 are separated by 21 nucleotides MMJJ_RS06420 and MMJJ_RS06425 are separated by 30 nucleotides MMJJ_RS06425 and MMJJ_RS06430 are separated by 268 nucleotides MMJJ_RS06430 and MMJJ_RS06435 are separated by 60 nucleotides
MMJJ_RS06415: MMJJ_RS06415 - 50S ribosomal protein L23, at 1,186,795 to 1,187,055
_RS06415
MMJJ_RS06420: MMJJ_RS06420 - 50S ribosomal protein L4, at 1,187,077 to 1,187,835
_RS06420
MMJJ_RS06425: MMJJ_RS06425 - 50S ribosomal protein L3, at 1,187,866 to 1,188,870
_RS06425
MMJJ_RS06430: MMJJ_RS06430 - haloacid dehalogenase, at 1,189,139 to 1,189,729
_RS06430
MMJJ_RS06435: MMJJ_RS06435 - tRNA-Ile, at 1,189,790 to 1,189,864
_RS06435
Position (kb)
1187
1188
1189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1187.500 kb on - strand at 1189.011 kb on + strand at 1189.041 kb on - strand at 1189.050 kb on + strand at 1189.065 kb on - strand at 1189.074 kb on + strand at 1189.099 kb on - strand at 1189.104 kb on + strand at 1189.115 kb on - strand at 1189.115 kb on - strand at 1189.116 kb on + strand at 1189.154 kb on - strand at 1189.158 kb on - strand at 1189.240 kb on + strand, within MMJJ_RS06430 at 1189.370 kb on - strand, within MMJJ_RS06430 at 1189.371 kb on + strand, within MMJJ_RS06430 at 1189.371 kb on + strand, within MMJJ_RS06430 at 1189.371 kb on + strand, within MMJJ_RS06430 at 1189.375 kb on + strand, within MMJJ_RS06430 at 1189.375 kb on + strand, within MMJJ_RS06430 at 1189.388 kb on - strand, within MMJJ_RS06430 at 1189.388 kb on - strand, within MMJJ_RS06430 at 1189.391 kb on - strand, within MMJJ_RS06430 at 1189.446 kb on - strand, within MMJJ_RS06430 at 1189.447 kb on + strand, within MMJJ_RS06430 at 1189.458 kb on + strand, within MMJJ_RS06430 at 1189.460 kb on + strand, within MMJJ_RS06430 at 1189.493 kb on + strand, within MMJJ_RS06430 at 1189.493 kb on + strand, within MMJJ_RS06430 at 1189.501 kb on + strand, within MMJJ_RS06430 at 1189.557 kb on + strand, within MMJJ_RS06430 at 1189.558 kb on - strand, within MMJJ_RS06430 at 1189.558 kb on - strand, within MMJJ_RS06430 at 1189.558 kb on - strand, within MMJJ_RS06430 at 1189.559 kb on + strand, within MMJJ_RS06430 at 1189.559 kb on + strand, within MMJJ_RS06430 at 1189.599 kb on + strand, within MMJJ_RS06430 at 1189.618 kb on - strand, within MMJJ_RS06430 at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.671 kb on - strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.672 kb on + strand at 1189.675 kb on - strand at 1189.675 kb on - strand at 1189.700 kb on + strand at 1189.700 kb on + strand at 1189.700 kb on + strand at 1189.700 kb on + strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.704 kb on - strand at 1189.705 kb on + strand at 1189.705 kb on + strand at 1189.727 kb on - strand at 1189.728 kb on + strand at 1189.728 kb on + strand at 1189.748 kb on + strand at 1189.753 kb on - strand at 1189.780 kb on - strand at 1189.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 1,187,500 - +0.5 1,189,011 + +0.9 1,189,041 - -1.7 1,189,050 + +0.5 1,189,065 - +0.2 1,189,074 + -0.7 1,189,099 - -1.7 1,189,104 + +0.1 1,189,115 - +1.6 1,189,115 - -1.1 1,189,116 + +1.4 1,189,154 - -0.1 1,189,158 - +0.9 1,189,240 + MMJJ_RS06430 0.17 -0.7 1,189,370 - MMJJ_RS06430 0.39 +0.0 1,189,371 + MMJJ_RS06430 0.39 +1.7 1,189,371 + MMJJ_RS06430 0.39 +2.4 1,189,371 + MMJJ_RS06430 0.39 +1.1 1,189,375 + MMJJ_RS06430 0.40 +3.3 1,189,375 + MMJJ_RS06430 0.40 -2.3 1,189,388 - MMJJ_RS06430 0.42 +1.3 1,189,388 - MMJJ_RS06430 0.42 +1.3 1,189,391 - MMJJ_RS06430 0.43 +2.2 1,189,446 - MMJJ_RS06430 0.52 -3.2 1,189,447 + MMJJ_RS06430 0.52 -0.6 1,189,458 + MMJJ_RS06430 0.54 -0.7 1,189,460 + MMJJ_RS06430 0.54 +0.4 1,189,493 + MMJJ_RS06430 0.60 -0.1 1,189,493 + MMJJ_RS06430 0.60 -1.4 1,189,501 + MMJJ_RS06430 0.61 +0.1 1,189,557 + MMJJ_RS06430 0.71 +0.5 1,189,558 - MMJJ_RS06430 0.71 -0.5 1,189,558 - MMJJ_RS06430 0.71 +0.5 1,189,558 - MMJJ_RS06430 0.71 +0.9 1,189,559 + MMJJ_RS06430 0.71 +2.4 1,189,559 + MMJJ_RS06430 0.71 +2.5 1,189,599 + MMJJ_RS06430 0.78 -0.3 1,189,618 - MMJJ_RS06430 0.81 -1.2 1,189,671 - +1.3 1,189,671 - +0.9 1,189,671 - +1.3 1,189,671 - +1.7 1,189,671 - -0.0 1,189,672 + +3.8 1,189,672 + +2.4 1,189,672 + +0.2 1,189,672 + -0.6 1,189,672 + -0.1 1,189,672 + +2.5 1,189,672 + +1.0 1,189,672 + +1.1 1,189,672 + -2.4 1,189,672 + +1.2 1,189,672 + +1.3 1,189,675 - -0.2 1,189,675 - +1.1 1,189,700 + +1.8 1,189,700 + -0.3 1,189,700 + -0.3 1,189,700 + -1.8 1,189,704 - +1.4 1,189,704 - -0.9 1,189,704 - -0.3 1,189,704 - +2.7 1,189,704 - -0.6 1,189,705 + -0.7 1,189,705 + -2.0 1,189,727 - -1.2 1,189,728 + +1.6 1,189,728 + +0.3 1,189,748 + +3.3 1,189,753 - +1.6 1,189,780 - +1.3 1,189,781 + +2.6
Or see this region's nucleotide sequence