Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03190 and MMJJ_RS03195 are separated by 241 nucleotides MMJJ_RS03195 and MMJJ_RS03200 are separated by 15 nucleotides MMJJ_RS03200 and MMJJ_RS03205 are separated by 58 nucleotides
MMJJ_RS03190: MMJJ_RS03190 - phosphopyruvate hydratase, at 604,641 to 605,933
_RS03190
MMJJ_RS03195: MMJJ_RS03195 - hypothetical protein, at 606,175 to 606,453
_RS03195
MMJJ_RS03200: MMJJ_RS03200 - uroporphyrinogen-III synthase, at 606,469 to 607,203
_RS03200
MMJJ_RS03205: MMJJ_RS03205 - metallophosphoesterase, at 607,262 to 607,777
_RS03205
Position (kb) 606
607 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 605.304 kb on - strand at 605.310 kb on - strand at 605.311 kb on + strand, within MMJJ_RS03190 at 605.340 kb on - strand, within MMJJ_RS03190 at 605.360 kb on + strand, within MMJJ_RS03190 at 605.498 kb on + strand, within MMJJ_RS03190 at 605.518 kb on - strand, within MMJJ_RS03190 at 605.581 kb on + strand, within MMJJ_RS03190 at 605.679 kb on - strand, within MMJJ_RS03190 at 605.971 kb on - strand at 605.998 kb on + strand at 606.027 kb on - strand at 606.043 kb on + strand at 606.099 kb on + strand at 606.106 kb on + strand at 606.118 kb on - strand at 606.122 kb on - strand at 606.122 kb on - strand at 606.123 kb on + strand at 606.123 kb on + strand at 606.124 kb on - strand at 606.142 kb on - strand at 606.155 kb on + strand at 606.155 kb on + strand at 606.172 kb on + strand at 606.195 kb on + strand at 606.195 kb on + strand at 606.198 kb on - strand at 606.216 kb on - strand, within MMJJ_RS03195 at 606.276 kb on + strand, within MMJJ_RS03195 at 606.346 kb on - strand, within MMJJ_RS03195 at 606.347 kb on + strand, within MMJJ_RS03195 at 606.414 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.422 kb on + strand, within MMJJ_RS03195 at 606.618 kb on + strand, within MMJJ_RS03200 at 606.775 kb on - strand, within MMJJ_RS03200 at 606.775 kb on - strand at 606.859 kb on + strand, within MMJJ_RS03200 at 607.078 kb on - strand, within MMJJ_RS03200 at 607.080 kb on - strand, within MMJJ_RS03200 at 607.182 kb on + strand at 607.193 kb on - strand at 607.214 kb on - strand at 607.214 kb on - strand at 607.215 kb on + strand at 607.215 kb on + strand at 607.215 kb on + strand at 607.217 kb on + strand at 607.220 kb on - strand at 607.225 kb on - strand at 607.225 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.232 kb on - strand at 607.233 kb on + strand at 607.236 kb on - strand at 607.236 kb on - strand at 607.236 kb on - strand at 607.236 kb on - strand at 607.237 kb on + strand at 607.237 kb on + strand at 607.245 kb on - strand at 607.248 kb on - strand at 607.262 kb on + strand at 607.273 kb on - strand at 607.295 kb on - strand at 607.295 kb on - strand at 607.296 kb on + strand at 607.296 kb on + strand at 607.299 kb on - strand at 607.300 kb on + strand at 607.307 kb on + strand at 607.308 kb on - strand at 607.309 kb on + strand at 607.348 kb on - strand, within MMJJ_RS03205 at 607.408 kb on + strand, within MMJJ_RS03205
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 605,304 - -0.5 605,310 - +0.5 605,311 + MMJJ_RS03190 0.52 -2.6 605,340 - MMJJ_RS03190 0.54 -0.7 605,360 + MMJJ_RS03190 0.56 +1.1 605,498 + MMJJ_RS03190 0.66 -0.5 605,518 - MMJJ_RS03190 0.68 -2.8 605,581 + MMJJ_RS03190 0.73 -0.5 605,679 - MMJJ_RS03190 0.80 -0.7 605,971 - +0.1 605,998 + -0.7 606,027 - +1.8 606,043 + -2.4 606,099 + -1.0 606,106 + -0.6 606,118 - -1.8 606,122 - -0.8 606,122 - -0.1 606,123 + -0.5 606,123 + -0.2 606,124 - -1.8 606,142 - -3.8 606,155 + +0.7 606,155 + -1.5 606,172 + -0.3 606,195 + -2.2 606,195 + -1.7 606,198 - -0.5 606,216 - MMJJ_RS03195 0.15 +0.4 606,276 + MMJJ_RS03195 0.36 -3.2 606,346 - MMJJ_RS03195 0.61 -2.9 606,347 + MMJJ_RS03195 0.62 -0.1 606,414 + MMJJ_RS03195 0.86 -0.6 606,422 + MMJJ_RS03195 0.89 +1.2 606,422 + MMJJ_RS03195 0.89 -0.3 606,422 + MMJJ_RS03195 0.89 -0.8 606,618 + MMJJ_RS03200 0.20 -0.9 606,775 - MMJJ_RS03200 0.42 -3.0 606,775 - +2.1 606,859 + MMJJ_RS03200 0.53 +1.1 607,078 - MMJJ_RS03200 0.83 +0.5 607,080 - MMJJ_RS03200 0.83 -0.1 607,182 + -0.5 607,193 - +0.7 607,214 - -2.2 607,214 - -2.2 607,215 + -0.6 607,215 + +0.4 607,215 + -3.0 607,217 + -0.1 607,220 - -2.8 607,225 - -3.0 607,225 - -3.0 607,232 - -1.4 607,232 - -3.0 607,232 - -4.1 607,232 - -3.0 607,232 - -1.8 607,233 + -0.4 607,236 - -1.7 607,236 - -1.9 607,236 - -2.3 607,236 - -0.3 607,237 + -0.7 607,237 + -1.7 607,245 - -0.5 607,248 - -1.1 607,262 + -3.7 607,273 - -1.6 607,295 - -2.5 607,295 - +1.0 607,296 + -2.2 607,296 + -3.6 607,299 - -2.9 607,300 + -2.8 607,307 + -1.8 607,308 - -4.6 607,309 + -3.1 607,348 - MMJJ_RS03205 0.17 -1.5 607,408 + MMJJ_RS03205 0.28 -2.8
Or see this region's nucleotide sequence